Compound containing basic group and use thereof

ABSTRACT

The present invention relates to a compound represented by formula (I-0): 
                         
wherein symbols in formula have the same meanings as described in the present specification, a salt thereof, an N-oxide thereof or a solvate thereof or a prodrug thereof, and medical use thereof. The compound of the present invention has an antagonistic activity against CXCR4 and is therefore useful as a preventive and/or therapeutic agent for CXCR4-mediated diseases, for example, inflammatory and immune diseases (for example, rheumatoid arthritis, arthritis, retinopathy, pulmonary fibrosis, transplanted organ rejection, etc.), allergic diseases, infections (for example, human immunodeficiency virus infection, acquired immunodeficiency syndrome, etc.), psychoneurotic diseases, cerebral diseases, cardiovascular disease, metabolic diseases, and cancerous disease (for example, cancer, cancer metastasis, etc.), or an agent for regeneration therapy.

TECHNICAL FIELD

The present invention relates to compounds having a basic group which isuseful as medicaments, and use thereof.

More specifically, the present invention relates to (1) compoundsrepresented by formula (I):

wherein all symbols have the same meanings as described hereinafter, andsalts thereof, N-oxides thereof or solvates thereof or prodrugs thereof,(2) use thereof, and (3) a method for producing the same.

BACKGROUND ART

Chemokine is known as a basic protein which has chemotaxis and anactivating activity against endogenous leucocytes and also has strongheparin-binding abilities. It is now considered that chemokine isassociated with not only control of infiltration of specific leucocytesupon inflammatory and immune responses, but also development, homing oflymphocytes under physiological conditions and migration of hemocyteprecursor cells and somatic cells.

Differentiation, proliferation and cell death of blood cells arecontrolled by various cytokines. Inflammation occurs at a local regionin a living body. Differentiation and maturation of lymphocytes, and thelike are carried out at a specific site. More particularly, requiredvarious cells migrate and accumulate in the specific site and a sequenceof inflammatory and immune responses arise. Thus, in addition todifferentiation, proliferation and death of cells, cell migration isalso an essential phenomenon to an immune system.

In the living body, migration of blood cells start with siftinghemopoiesis that started at AGM (Aorta Gonad Mesonephros) region viafetal liver to permanent hematopoiesis at bone marrow in a developmentcourse. Moreover, precursors of T cells and thymus dendritic cellsmigrate from fetal liver into bone marrow and then into the thymusgland. They differentiate under thymus environment. The T cells aresubjected to clonal selection migrates into secondary lymphoid tissues,where they contribute to immune responses in periphery. Skin Langerhanscells that caught antigen, thereby undergone activation anddifferentiation migrate to T cell region in a topical lymph node, wherethey activate naive T cells therein as dendritic cells. The memory Tcells again perform its homing into the lymph node via lymphatic andblood vessels. In addition, B cells, T cells in intestinal epithelia,γδT cells, NKT cells, and dendritic cells migrate from bone marrow notvia thymus, differentiate and contribute to immune responses.

Chemokine is closely associated with such a migration of the variouscells. For example, SDF-1 (Stromal cell derived factor-1) and itsreceptor, CXCR4 also act on various immune- and inflammatory reactions.For example, they have been reported to be associated with accumulationand activation of CD4+T cells in a synovial membrane from a humanpatient suffering from rheumatoid arthritis (J. Immunol., 165, 6590-6598(2000)). In addition, in a CIA model mouse, CXCR4 inhibitor inhibitedaccumulation of leucocytes in a joint and dramatically reduced arthritisscore (J. Immunol., 167, 4648-4692 (2001)). In a mouse OVA-inducedairway hypersensitive model, an anti-CXCR4 antibody reduced the numberof eosinophiles accumulating in pulmonary interstitial tissues andprevented airway hypersensitivity (J. Immunol., 165, 499-508 (2000)).

There has been reported that SDF-1 and its receptor, CXCR4 play animportant role in maintaining hemopoietic stem cells in bone marrow J.Exp. Med., 185, 111-120 (1997), Blood, 97, 3354-3360 (2001)).Accordingly, control of SDF-1 and CXCR4 is expected to modulaterecruitment of hemopoietic stem cells to peripheral blood and are usefulfor peripheral blood stem cell transplantation and reproductiontransplantation treatment.

SDF-1 and CXCR4 are associated with infiltration of various cancer cellssuch as breast cancer, prostate cancer, and ovarian cancer (Nature, 410,50-56 (2001), Cancer Res., 62, 1832-1837 (2002), Cancer Res., 62,5930-5938 (2002)). In a model of transferring a human breast cancer cellstrain into a SCID mouse, an anti-CXCR4 antibody prevented metastasis ofbreast cancer cells to lung (Nature, 410, 50-56 (2001)). In humanovarian epithelial tumor, highly expression of SDF-1 promotesaccumulation of plasmacytoid dendritic cells and inhibits the act ofbone marrow dendritic cells associated with tumor immune and suppressestumor immune (Nat. Med., 12, 1339 (2001)). Moreover, SDF-1 is associatedwith proliferation and migration of non-Hodgkin's lymphoma cells, and ina model of transferring a human non-Hodgkin's lymphoma cells into aNOD/SCID mouse, an anti-CXCR4 antibody inhibited proliferation of thetumor cells and improved mouse mortality (Cancer Res., 62, 3106-3112(2002)).

SDF-1 and CXCR4 play an important role for formation of hippocampusdentate gyrus granulocyte, that is essential for memory and learning andare associated with development of a disease associated with adultplasticity and pathology of hippocampus, for example Alzheimer'sdisease, stroke and epilepsy (Development, 129, 4249-4260 (2002), Trendsin Neuroscience, 25, 548-549 (2002)).

SDF-1 and CXCR4 are essential for a function of self-reactive B cellsassociated with development of diabetes. In NOD mouse, an anti-SDF-1antibody reduced blood glucose level and the number of mature IgM+Bcells in a periphery tissue (Immunology, 107, 222-232 (2002)). In ahuman arteriosclerotic plaque, SDF-1 was highly expressed and activatedblood platelets (Circ. Res., 86, 131-138 (2000)).

SDF-1 and CXCR4 are associated with residence of hemopoietic stem cellsand hemopoietic precursor cells in bone marrow. CXCR4 antagonist, AMD3100 in combination with G-CSF increased the numbers of hemopoietic stemcells and hemopoietic precursor cells in periphery blood (JournalExperimental Medicine, 2001, 1307-1318 (2005)).

In addition, the results of SDF-1/CXCR4 knock-out mice showed that SDF-1is essential for functions of central nervous system, heart and vesselsof gastrointestinal tract in addition to lymphocytes (Nature, 382,635-639 (1996), Nature, 393, 591-594 (1998), Nature, 393, 595-599(1998)). Accordingly, it may be associated with a disease of thesetissues.

Thus, chemokine receptors are expressed at various specific cells and ata specific time. They are largely associated with the control ofinflammatory- and immune-responses through a mechanism by which theireffector cells accumulate in a site where chemokine is produced.

Acquired immunodeficiency syndrome (also called AIDS) that caused byinfection of human immunodeficiency virus (hereinafter abbreviated toHIV) is one of diseases for which therapies are the most eagerly desiredlately. Once HIV infection has been established in a main target cell,CD4+ cell, HIV repetitively proliferates in a patient's body and in theevent deathly destroys T cells responsible for immunological functionsby necrosis. In this process, immunological functions are graduallydeteriorated, various immunocompromised states become to develop such asfever, diarrhea and swelling of a lymph node, and various opportunisticinfections such as carinii pneumonia are easily complicated. It is wellknown that such a state is the onset of AIDS and induces malignanttumors such as Kaposi's sarcoma and becomes severe.

Currently, there are tried various preventive and therapeutic treatmentsfor AIDS as follows: for example, (1) inhibition of HIV proliferation byadministration of reverse transcriptase inhibitors and proteaseinhibitors, and (2) prevention or alleviation of opportunisticinfections by administration of an immunostimulant, etc.

HIV mainly infects helper T cells which play a key role in the immunesystem. Since 1985, it has been known that in this process HIV utilizesa membrane protein CD4 that is expressed on the membrane of T cells(Cell, 52, 631 (1985)). CD4 molecule consists of 433 amino acid residuesand is expressed in macrophages, some B cells, vascular endothelialcells, Langerhans cells in skin tissues, dendritic cells located inlymphatic tissues, glia cells of central nervous system and the like inaddition to mature helper T cells. However, as it becomes obvious thatHIV infection cannot be established with only CD4 molecule, the possiblepresence of some factor that is responsible for infection of cell withHIV, other than CD4 molecule, has been suggested.

In 1996, a cell membrane protein called Fusin has been identified as afactor responsible for HIV infection other than a CD4 molecule (Science,272, 872 (1996)). This Fusin molecule has been demonstrated to be areceptor for SDF-1, namely, CXCR4. In addition, it has been shown thatSDF-1 specifically inhibits infection of T cell-directed (X4) HIV invitro (Nature, 382, 829 (1996), Nature, 382, 833 (1996)). This may beconsidered that SDF-1 binds to CXCR4 prior to HIV, thereby taking away ascaffold for infecting a cell from HIV resulting in inhibition of HIVinfection.

Also, at the same period, there has been found that another chemokinereceptor CCR5, that is a receptor for RANTES, MIP-1α, and MIP-1β, isutilized at infection of macrophage-directed (R5) HIV (Science, 272,1955 (1996)).

Accordingly, those which can compete with HIV for CXCR4 and CCR5 orthose which bind to a HIV virus and prevent for said virus from bindingto CXCR4 and CCR5 may be a HIV infection inhibitor. In addition, thereis a case where a low molecular weight compound discovered as a HIVinfection inhibitor was showed to be indeed an antagonist of CXCR4(Nature Medicine, 4, 72 (1998)).

As described above, compounds having an antagonistic activity againstCXCR4 is effective, such as, for prevention and/or treatment ofinflammatory and immune diseases, allergic diseases, infections,particularly HIV infection, and diseases associated with the infection,psychoneurotic diseases, cerebral diseases, cardiovascular diseases,metabolic diseases, cancerous diseases and the like. Also, the compoundsare useful for cell medical treatment and regeneration therapy.

Heretofore, some compounds having an antagonistic activity against CXCR4have been reported. For example, it is disclosed that a compoundrepresented by formula (X):

wherein A^(X) represents

A^(1X) and A^(2X) each independently represents a hydrogen atom, anoptionally substituted monocyclic or polycyclic heteroaromatic ring, oran optionally substituted monocyclic or polycyclic aromatic ring;

G^(1X) represents a single bond or —CR^(2X)R^(3X)—;

R^(1X), R^(2X) and R^(3X) represent an optionally substituted alkylgroup having 1 to 6 carbon atom(s);

W^(X) represents an optionally substituted alkylene group having 1 to 7carbon atom(s), an optionally substituted monocyclic or polycyclicheteroaromatic ring, or an optionally substituted monocyclic orpolycyclic aromatic ring;

x^(X) represents -z^(1X)-CO-z^(2X)-;

z^(1X) and z^(2X) each independently represents a single bond orNR^(13X);

y^(X) represents —CO—;

D^(1X) and D^(2X) each independently represents a hydrogen atom or-G^(2X)-R^(4X); G^(2X) represents an optionally substituted alkylenegroup having 1 to 15 carbon atom(s);

R^(4X) represents a hydrogen atom, an optionally substituted monocyclicor polycyclic heteroaromatic ring, or an optionally substitutedmonocyclic or polycyclic aromatic ring;

n2X represents 0 to 4;

n1X represents 0 to 3; and

BX represents —NR^(6X)R^(7X), and only required portions were extractedwith respect to definition of each group), or a pharmaceuticallyacceptable salt has an antagonistic activity against CXCR4 (seeWO03/029218 pamphlet).

Also, it is disclosed that a compound represented by formula (Y):

wherein n1Y, n2Y and n3Y represent 0 to 3;

R^(1Y), R^(2Y), R^(3Y), R^(4Y), R^(5Y) and R^(6Y) each independentlyrepresents a hydrogen atom, or an optionally substituted alkyl grouphaving 1 to 15 carbon atom(s);

A^(1Y) and A^(2Y) each independently represents an optionallysubstituted monocyclic or polycyclic heteroaromatic ring;

W^(Y) represents an optionally substituted alkylene group having 1 to 15carbon atom(s); X^(Y) represents O, CH₂, or NR^(11Y);

D^(Y) represents -Q^(Y)-Y^(Y)—B^(Y);

Q^(Y) represents a single bond or —CO— when X^(Y) is NR^(11Y);

Y^(Y) represents —(CR^(18Y)R^(19Y))_(m3Y)—;

R^(18Y) and R^(19Y) each independently represents a hydrogen atom, or anoptionally substituted alkyl group having 1 to 15 carbon atom(s);

m3Y represents 0 to 6; B^(Y) represents —NR^(25Y)R²⁶Y; and

R^(25Y) and R^(26Y) represent a hydrogen atom or an optionallysubstituted alkyl group having 1 to 15 carbon atom(s) when X^(Y) is notCH₂, and only required portions were extracted with respect todefinition of each group), or a pharmaceutically acceptable salt or aprodrug thereof has an antagonistic activity against CXCR4 (seeWO2004/024697 pamphlet).

DISCLOSURE OF THE INVENTION

It is earnestly desired to develop a CXCR4 antagonist which is useful asa preventive and/or therapeutic agent for inflammatory and immunediseases (for example, rheumatoid arthritis, arthritis, retinopathy,pulmonary fibrosis, transplanted organ rejection, etc.), allergicdiseases, infections (for example, human immunodeficiency virusinfection, acquired immunodeficiency syndrome, etc.), and cancerousdiseases (for example, cancer, cancer metastasis, etc.), or an agent forregeneration therapy, and also causes less side effect and is safe.

The present inventors have intensively studied and found that a compoundrepresented by formula (I-0) described hereinafter surprisingly has astrong antagonistic activity against CXCR4, and thus the presentinvention has been completed.

Namely, the present invention relates to:

[1] A compound represented by formula (I-0):

wherein A¹ and A² each independently represents a group having a basicgroup;

B¹ and B² each independently represents a bond or a spacer having 1 to 4atom(s) in its main chain;

E represents a spacer having 1 to 10 atom(s) in its main chain; Lrepresents a bond or a spacer having 1 to 4 atom(s) in its main chain;

J⁰ represents (1) an aliphatic hydrocarbon group which is substitutedwith a group having a basic group, and also may have a substituent(s),(2) a cyclic group which is substituted with a group having a basicgroup, and also may have a substituent(s), (3) a spiro-bound cyclicgroup which may be substituted with a group having a basic group, andalso may have a substituent(s), or (4) a bridged cyclic group which maybe substituted with a group having a basic group, and also may have asubstituent(s);

represents a single bond or a double bond;

when

represents a single bond, D represents a nitrogen atom or a carbon atomwhich may have a substituent(s), G represents a carbon atom which mayhave a substituent(s), a carbonyl group, an oxygen atom, a nitrogen atomwhich may have a substituent, or a sulfur atom which may be oxidized,

when

represents a double bond, D represents a carbon atom; and G represents acarbon atom which may have a substituent(s), or a nitrogen atom, andwherein when J⁰ represents the above group (1) or (2) and also Drepresents a nitrogen atom, G represents a carbonyl group, an oxygenatom, a nitrogen atom which may have a substituent, or a sulfur atomwhich may be oxidized, or B¹ represents a spacer having 1 to 4 atom(s)in its main chain, which has at least one selected from —O—, —S—, —CO—,—SO—, —SO₂—, and a divalent nitrogen atom which may have a substituent)or a salt thereof, an N-oxide thereof or a solvate thereof, or a prodrugthereof;

[2] The compound according to the above-described [1], which isrepresented by formula (I):

wherein J has the same meaning as in (1) or (2) in J⁰ described in [1],and other symbols have the same meanings as described in [1], and

wherein when D represents a nitrogen atom, G represents a carbonylgroup, an oxygen atom, a nitrogen atom which may have a substituent, ora sulfur atom which may be oxidized, or B¹ represents a spacer having 1to 4 atom(s) in its main chain, which has at least one selected from—O—, —S—, —CO—, —SO—, —SO₂—, and a divalent nitrogen atom which may havea substituent;

[3] The compound according to the above-described [1], wherein J⁰ is (3)or (4) described in the above [1]:

[4] The compound according to the above-described [3], wherein J⁰ is aspiro-bound heterocyclic ring or bridged heterocyclic ring which may besubstituted with a group having a basic group, and also may have atleast one nitrogen atom which may have a substituent, and also may havean oxygen atom(s) and/or a sulfur atom(s) which may be oxidized;[5] The compound according to the above-described [3], wherein J⁰ is aspiro-bound carbocyclic ring or bridged carbocyclic ring which may besubstituted with a group having a basic group, and also may have asubstituent(s);[6] The compound according to the above-described [4], wherein thespiro-bound heterocyclic ring or bridged heterocyclic ring which mayhave at least one nitrogen atom and also may have an oxygen atom(s)and/or a sulfur atom(s) which may be oxidized is

[7] The compound according to the above-described [4], wherein the ringconstituting the spiro-bound heterocyclic ring is a 7- to 15-memberedbicyclic spiro-bound heterocyclic ring comprising a monocycle having atleast one nitrogen atom and carbon atom(s), and/or a monocycle having atleast one nitrogen atom, one oxygen atom and carbon atoms;[8] The compound according to the above-described [7], wherein the 7- to15-membered bicyclic spiro-bound heterocyclic ring is

[9] The compound according to the above-described [5], wherein thespiro-bound carbocyclic ring or bridged carbocyclic ring is

[10] The compound according to the above-described [1], wherein A¹ andA² each independently represents a nitrogen-containing heterocyclic ringwhich may have a substituent(s);[11] The compound according to the above-described [10], wherein thenitrogen-containing heterocyclic ring is an imidazole ring or abenzimidazole ring;[12] The compound according to the above-described [1], wherein thespacer having 1 to 4 atom(s) in its main chain represented by B¹ and B²each independently represents —CO—, —SO₂— or —CH₂—;[13] The compound according to the above-described [1], wherein D is anitrogen atom;[14] The compound according to the above-described [1], wherein G is—CO—, —SO₂—or —CH₂—;[15] The compound according to the above-described [1], wherein E is adivalent 3- to 8-membered monocyclic cyclic group which may have asubstituent(s);[16] The compound according to the above-described [15], wherein the 3-to 8-membered monocyclic cyclic group is a benzene ring;[17] The compound according to the above-described [1], wherein L is—CH₂—, —CONH— or —CH₂—NH— in which J^(o) is bound to the right side ofeach group;[18] The compound according to the above-described [2], wherein thebasic group is a mono- or di-substituted amino group;[19] The compound according to the above-described [18], wherein thesubstituent of the di-substituted amino group is a C1-8 alkyl groupand/or a cyclic group;[20] The compound according to the above-described [19], wherein thedi-substituted amino group is a dipropylamino group, or anN-cyclohexyl-N-propylamino group;[21] The compound according to the above-described [2], wherein thealiphatic hydrocarbon group is a butyl group, or the cyclic group is acyclohexyl group;[22] The compound according to the above-described [2], which isrepresented by formula (I-3):

wherein ring A^(1A) and ring A^(2A) each independently represents anitrogen-containing heterocyclic ring which may have a substituent(s);

B^(1A), B^(2A) and G^(A) each independently represents —CO—, —SO₂— or—CH₂—;

ring E^(A) represents a divalent 3- to 8-membered monocyclic cyclicgroup which may have a substituent(s), or a divalent 9- or 10-memberedpolycyclic cyclic group which may have a substituent(s);

L has the same meaning as described in [1]; and

J has the same meaning as described in [2], and

wherein any of B^(1A) and G^(A) represents —CO— or —SO₂—;

[23] The compound according to the above-described [22], wherein L is abond, —CH₂—, —CONH—, —S—CH₂—, or —CH₂—CH₂— in which J is bound to theright side of each group;

[24] The compound according to the above-described [1], which isrepresented by formula (I-4):

wherein ring A^(1B) and ring A^(2B) each independently representsimidazole or benzimidazole which may have a substituent(s),

ring E^(A) has the same meaning as described in [22], and

other symbols have the same meanings as described in [1]:

[25] The compound according to the above-described [1], wherein-L-J⁰ is

in the group,

(a) when L^(A) is -(a C1-3 aliphatic hydrocarbon group which may have asubstituent(s))-(nitrogen atom which may have a substituent)-, ring J¹represents (i) a C3-10 monocyclic or bicyclic carbocyclic ring, or (ii)a 3- to 10-membered monocyclic or bicyclic heterocyclic ring comprisinga carbon atom(s), an oxygen atom(s) and/or a sulfur atom(s) which may beoxidized,

(b) when L^(A) is a divalent C1-4 aliphatic hydrocarbon group which mayhave a substituent(s), ring J¹ represents a 3- to 10-membered monocyclicor bicyclic heterocyclic ring which may have at least one nitrogen atomand also may have an oxygen atom(s) and/or a sulfur atom(s) which may beoxidized,

ring J² represents (i) a C3-10 monocyclic or bicyclic carbocyclic ringsubstituted with a group having a basic group; (ii) a 3- to 10-memberedmonocyclic or bicyclic heterocyclic ring which may be substituted with agroup having a basic group and which comprises a carbon atom(s), anoxygen atom(s) and/or a sulfur atom(s) which may be oxidized; or (iii) a3- to 10-membered monocyclic or bicyclic heterocyclic ring which may besubstituted with a group having a basic group, and also may have atleast one nitrogen atom, and also may have an oxygen atom(s) or a sulfuratom(s) which may be oxidized, and

ring J¹ and ring J² may have 1 to 8 substituent(s) at the substitutableposition and, when two or more substituents are present, pluralsubstituents may be the same or different,

wherein the nitrogen atom which may have a substituent in L^(A) is boundto ring J¹;

[26] The compound according to the above-described [25], wherein

is:

in the group, L^(A) has the same meaning as described in [25], whereinL^(A) may be bound to a nitrogen atom of —NH— and the nitrogen atom of—NH— may have a substituent;

[27] The compound according to the above-described [25], wherein

is:

in the group, L^(A) has the same meaning as described in [25], whereinL^(A) may be bound to a nitrogen atom of —NH— and the nitrogen atom of—NH— may have a substituent;

[28] The compound according to the above-described [24], which isrepresented by formula (I-4-1a):

or formula (I-4-1b):

wherein L^(A1) represents a divalent C1-4 aliphatic hydrocarbon groupwhich may have a substituent(s);

R¹ represents a hydrogen atom or a substituent; and

other symbols have the same meanings as described in [24];

[29] The compound according to the above-described [24], which isrepresented by formula (I-4-2):

wherein all symbols have the same meanings as described in [24] and[28];

[30] The compound according to the above-described [24], which isrepresented by formula (I-4-3a):

or formula (I-4-3b):

wherein all symbols have the same meanings as described in [24] and[28];

[31] The compound described in [24], which is represented by formula(I-4-4):

wherein all symbols have the same meanings as described in [24] and[28];

[32] The compound according to the above-described [24], which isrepresented by formula (I-4-5):

wherein all symbols have the same meanings as described in [24] and[28];

[33] The compound according to the above-described any one of [28] to[32], wherein the substituent represented by R¹ is an aliphatichydrocarbon group, a cyclic group, or an aliphatic hydrocarbon groupsubstituted with a cyclic group;

[34] The compound according to the above-described [24], wherein-L-J⁰ is

in the group, ring J³ represents (i) a bridged polycyclic carbocyclicring substituted with a group having a basic group; (ii) a bridgedpolycyclic heterocyclic ring substituted with a group having a basicgroup, which is composed of a carbon atom, an oxygen atom and/or asulfur atom which may be oxidized; or (iii) a bridged polycyclicheterocyclic ring which may be substituted with a group having a basicgroup, and may have at least one nitrogen atom and also may have anoxygen atom and/or a sulfur atom which may be oxidized;

ring J³ may have 1 to 8 substituent(s) on the substitutable positionand, when two or more substituents are present, plural substituents arethe same or different; and

L^(A) has the same meaning as described in [25];

[35] The compound according to the above-described [34], wherein thebridged polycyclic carbocyclic ring is

[36] The compound according to the above-described [34], wherein thebridged polycyclic heterocyclic ring which has at least one nitrogenatom and also may have an oxygen atom and/or a sulfur atom which may beoxidized is

[37] The compound according to the above-described [24], wherein-L-J⁰ is

in the group, ring J⁴ represents (i) a C3-15 monocyclic or condensedcarbocyclic ring substituted with a group having a basic group; (ii) a3- to 15-membered monocyclic or condensed heterocyclic ring substitutedwith a group having a basic group, which is composed of a carbon atom,an oxygen atom and/or a sulfur atom which may be oxidized; or (iii) a 3-to 15-membered monocyclic or condensed heterocyclic ring which may besubstituted with a group having a basic group, and also may have atleast one nitrogen atom and also may have an oxygen atom and/or a sulfuratom which may be oxidized,

ring J⁴ may have 1 to 8 substituent(s) on the substitutable positionand, when two or more substituents are present, plural substituents arethe same or different; and

L^(A) has the same meaning as described in [25]);

[38] The compound according to the above-described [37], wherein theC3-15 monocyclic or condensed carbocyclic ring is

[39] The compound according to the above-described [38], wherein theC3-15 monocyclic or condensed carbocyclic ring is a cyclohexyl group;[40] The compound according to the above-described [24], which isrepresented by formula (I-4-6):

wherein L^(A2) represents a C1-3 aliphatic hydrocarbon group which mayhave a substituent(s);

R², R³ and R⁴ each independently represent a hydrogen atom or asubstituent; and

R² and R³ may be combined with the nitrogen atom to which they areattached to form a ring which may have a substituent(s); and

other symbols have the same meanings as described in [24];

[41] The compound according to the above-described [40],

wherein R² and R³ each independently represents a hydrogen atom, analiphatic hydrocarbon group, a C3-15 monocyclic or condensed carbocyclicring, or an aliphatic hydrocarbon group substituted with a C3-15monocyclic or condensed carbocyclic ring, or may be combined with thenitrogen atom to which R² and R³ are attached to form a monocyclic orcondensed heterocyclic ring; and

wherein an atom(s) other than a nitrogen atom, which constitutes aheterocyclic ring, is/are a carbon atom;

[42] The compound described in [40], wherein R⁴ is a hydrogen atom, analiphatic hydrocarbon group, or an aliphatic hydrocarbon groupsubstituted with a hydroxyl group;

[43] The compound according to the above-described [37], wherein the 3-to 15-membered monocyclic or condensed heterocyclic ring which has atleast one nitrogen atom and also may have an oxygen atom and/or a sulfuratom which may be oxidized is

[44] The compound according to the above-described [1], which isrepresented by formula (I-8):

wherein ring A^(1A), ring A^(2A) and ring E^(A) have the same meaningsas described in [22];

L^(A) has the same meaning as described in [25]; and

J⁰ has the same meaning as described in (3) or (4) in J⁰ described in[1];

[45] The compound according to the above-described [44], wherein-L^(A)-J⁰- is:

in the group, L^(A1) and R¹ have the same meanings as described in [28];

[46] The compound according to the above-described [45], wherein thesubstituent represented by R¹ is an aliphatic hydrocarbon group, acyclic group, or an aliphatic hydrocarbon group substituted with acyclic group;

[47] The compound according to the above-described [1], which is

-   4-({[4-(dipropylamino)butyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;-   4-({[3-(dibutylamino)propyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;-   4-({[5-(dipropylamino)pentyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;-   4-({4-[(dipropylamino)methyl]-1-piperidinyl}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;-   4-({[4-(dipropylamino)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;-   4-({[3-(dipropylamino)propyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;-   4-[(2-benzyl-2,9-diazaspiro[5.5]undec-9-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;-   4-[(2-cyclohexyl-2,9-diazaspiro[5.5]undec-9-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;-   2-[4-(dipropylamino)butyl]-N,N-bis(1H-imidazol-2-ylmethyl)-1,2,3,4-tetrahydro-6-isoquinolinecarboxamide;-   N-[4-({[4-(dipropylamino)butyl]amino}methyl)benzyl]-N-(1H-imidazol-2-ylmethyl)-1H-imidazole-2-carboxamide;-   4-({[4-(dipropylamino)butyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzenesulfonamide;-   4-[({trans-4-[cyclohexyl(propyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;-   4-[(2-cyclohexyl-2,8-diazaspiro[4.5]dec-8-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;    or-   4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;    [48] The compound according to the above-described [1], which is-   4-[(2-cyclohexyl-2,8-diazaspiro[4.5]dec-8-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;-   4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;-   4-[(2-cyclohexyl-2,8-diazaspiro[4.5]dec-8-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;-   4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;-   N,N-bis(1H-imidazol-2-ylmethyl)-4-{[8-(2-thienylmethyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}benzamide;-   N-(1H-benzoimidazol-2-ylmethyl)-4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N-(1H-imidazol-2-ylmethyl)benzamide;-   4-[(8-cycloheptyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;-   N,N-bis(1H-imidazol-2-ylmethyl)-4-({8-[(3-methyl-2-thienyl)methyl]-2,8-diazaspiro[4.5]dec-2-yl}methyl)benzamide;    or-   4-{[8-(1-ethylpropyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;    [49] The compound according to the above-described [1], which is-   4-[({trans-4-[cyclohexyl(propyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;-   4-[({trans-4-[benzyl(propyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;-   4-({[4-(1-azepanyl)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;-   N-(1H-benzoimidazol-2-ylmethyl)-4-[({trans-4-[cyclohexyl(propyl)amino]cyclohexyl}amino)methyl]-N-(1H-imidazol-2-ylmethyl)benzamide;-   4-[({trans-4-[(1-ethylpropyl)(propyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;-   4-{[{trans-4-[cyclohexyl(propyl)amino]cyclohexyl}(methyl)amino]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;-   4-{[{trans-4-[cyclohexyl(propyl)amino]cyclohexyl}(2-hydroxyethyl)amino]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;    or-   4-[({trans-4-[(1-ethylpropyl)(2-hydroxyethyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;    [50] A pharmaceutical composition comprising a compound represented    by formula (I-0) described in [1], a salt thereof, an N-oxide    thereof or a solvate thereof, or a prodrug thereof;    [51] The pharmaceutical composition according to the above-described    [50], which is a CXCR4 antagonist;    [52] The pharmaceutical composition described in [51], which is an    agent for preventing and/or treating CXCR4-mediated diseases, or an    agent for regeneration therapy;    [53] The pharmaceutical composition according to the above-described    [52], wherein the CXCR4-mediated disease is human immunodeficiency    virus infection, acquired immunodeficiency syndrome, cancer, cancer    metastasis, rheumatoid arthritis, arthritis, retinopathy, pulmonary    fibrosis or transplanted organ rejection, or the agent for    regeneration therapy is an agent for transplantation medical    treatment;    [54] The pharmaceutical composition according to the above-described    [52], wherein the CXCR4-mediated disease is human immunodeficiency    virus infection;    [55] A pharmaceutical composition comprising a compound represented    by formula (I-0) depicted in [1], a salt thereof, an N-oxide thereof    or a solvate thereof, or a prodrug thereof, and one or more kinds    selected from reverse transcriptase inhibitor, protease inhibitor,    CCR2 antagonist, CCR3 antagonist, CCR4 antagonist, CCR5 antagonist,    CXCR4 antagonist, HIV integrase inhibitor, fusion inhibitor, CD4    antagonist, antibody against surface antigen of HIV, short    interfering RNA, and vaccine of HIV;    [56] A method for antagonizing CXCR4 in a mammal, which comprises    administering an effective dosage of a compound represented by    formula (I-0) depicted in [1], a salt thereof, an N-oxide thereof or    a solvate thereof, or a prodrug thereof to the mammal:    [57] A method of preventing and/or treating CXCR4-mediated diseases    in a mammal, which comprises administering an effective dosage of a    compound represented by formula (I-0) depicted in [1], a salt    thereof, an N-oxide thereof or a solvate thereof, or a prodrug    thereof to the mammal;    [58] Use of a compound represented by formula (I-0) described in    [1], a salt thereof, an N-oxide thereof or a solvate thereof or a    prodrug thereof for production of a CXCR4 antagonist;    [59] Use of a compound represented by formula (I-0) depicted in [1],    a salt thereof, an N-oxide thereof or a solvate thereof or a prodrug    thereof for production of an agent for preventing and/or treating    CXCR4-mediated diseases;    [60] A compound represented by formula (1):

wherein A¹ and A² each independently represents a group having a basicgroup;

B¹ and B² each independently represents a bond or a spacer having 1 to 4atom(s) in its main chain;

E represents a spacer having 1 to 10 atom(s) in its main chain;

L represents a bond or a spacer having 1 to 2 atom(s) in its main chain;

J represents an aliphatic hydrocarbon group which is substituted with agroup having a basic group, and also may be substituted by asubstituent(s), or a cyclic group which is substituted with a grouphaving a basic group, and also may be substituted by a substituent(s);

represents a single bond or a double bond;

when

represents a single bond, D represents a nitrogen atom or a carbon atomwhich may have a substituent(s), G represents a carbon atom which mayhave a substituent(s), a carbonyl group, an oxygen atom, or a sulfuratom which may be oxidized,

when

represents a double bond, D represents a carbon atom, and G represents acarbon atom which may have a substituent(s), or a nitrogen atom;

wherein when D represents a nitrogen atom, G represents a carbonylgroup, an oxygen atom, or a sulfur atom which may be oxidized, or B¹represents a spacer having a main chain of 1 to 4 atom(s), which has atleast one selected from —O—, —S—, —CO—, —SO—, —SO₂—, a divalent nitrogenatom which may have a substituent and a divalent 3- and 8-memberedmonocyclic cyclic group which may have a substituent(s)) or a saltthereof, an N-oxide thereof or a solvate thereof, or a prodrug thereof;or

[61] A method for producing a compound represented by formula (I-0), asalt thereof, an N-oxide thereof or a solvate thereof, or a prodrugthereof.

In the present specification, “cyclic group” includes, for example, acarbocyclic ring or a heterocyclic ring. Examples of the “carbocyclicring” include C3-15 monocyclic or polycyclic carbocyclic ring. The“C3-15 monocyclic or polycyclic carbocyclic ring” includes a C3-15monocyclic or polycyclic unsaturated carbocyclic ring, or partially orcompletely saturated one thereof, a spiro-bound polycyclic carbocyclicring and a bridged polycyclic carbocyclic ring. Examples of the “C3-15monocyclic or polycyclic unsaturated carbocyclic ring, or partially orcompletely saturated one thereof” include cyclopropane, cyclobutane,cyclopentane, cyclohexane, cycloheptane, cyclooctane, cyclononane,cyclodecane, cycloundecane, cyclododecane, cyclotridecane,cyclotetradecane, cyclopentadecane, cyclopentene, cyclohexene,cycloheptene, cyclooctene, cyclopentadiene, cyclohexadiene,cycloheptadiene, cyclooctadiene, benzene, pentalene, perhydropentalene,azulene, perhydroazulene, indene, perhydroindene, indane, naphthalene,dihydronaphthalene, tetrahydronaphthalene, perhydronaphthalene,heptalene, perhydroheptalene, biphenylene, as-indacene, s-indacene,acenaphthylene, acenaphthene, fluorene, phenalene, phenanthrene,anthracene, and 1,2,3,5,6,7-hexahydro-s-indacene rings; examples of the“spiro-bound polycyclic carbocyclic ring” include spiro[4.4]nonane,spiro[4.5]decane, spiro[5.5]undecane, spiro[3.4]octane, andspiro[3.5]nonane rings; and examples of the “bridged polycycliccarbocyclic ring” include bicyclo[2.2.1]heptane,bicyclo[2.2.1]hept-2-ene, bicyclo[3.1.1]heptane,bicyclo[3.1.1]hept-2-ene, bicyclo[3.2.1]octane, bicyclo[2.2.2]octane,bicyclo[2.2.2]oct-2-ene, adamantane, noradamantane,bicyclo[2.1.1]hexane, bicyclo[3.3.1]nonane, bicyclo[3.2.1]octane,bicyclo[3.3.2]decane ring. Among these, examples of the “C3-15monocyclic or polycyclic aromatic carbocyclic ring” include benzene,azulene, naphthalene, phenanthrene, and anthracene rings.

Examples of the heterocyclic ring includes “3- to 15-membered monocyclicor polycyclic heterocyclic ring having, as a hetero atom, 1 to 4nitrogen atom(s), 1 to 2 oxygen atom(s) and/or 1 to 2 sulfur atom(s)”.The “3- to 15-membered monocyclic or polycyclic heterocyclic ringhaving, as a hetero atom, 1 to 4 nitrogen atom(s), 1 to 2 oxygen atom(s)and/or 1 to 2 sulfur atom(s)” includes, 3- to 15-membered monocyclic orpolycyclic unsaturated heterocyclic ring having, as a hetero atom, 1 to4 nitrogen atom(s), 1 to 2 oxygen atom(s) and/or 1 to 2 sulfur atom(s),or partially or completely saturated one thereof, a spiro-boundpolycyclic heterocyclic ring and a bridged polycyclic heterocyclic ring.Examples of the “3- to 15-membered monocyclic or polycyclic unsaturatedheterocyclic ring having, as a hetero atom, 1 to 4 nitrogen atom(s), 1to 2 oxygen atom(s) and/or 1 to 2 sulfur atom(s), or partially orcompletely saturated one thereof” include pyrrole, imidazole, triazole,tetrazole, pyrazole, pyridine, pyrazine, pyrimidine, pyridazine,azepine, diazepine, furan, pyran, oxepine, thiophene, thiopyran,thiepine, oxazole, isoxazole, thiazole, isothiazole, furazan,oxadiazole, oxazine, oxadiazine, oxazepine, oxadiazepine, thiadiazole,thiazine, thiadiazine, thiazepine, thiadiazepine, indole, isoindole,indolizine, benzofuran, isobenzofuran, benzothiophene,isobenzothiophene, dithianaphthalene, indazole, quinoline, isoquinoline,quinolizine, purine, phthalazine, pteridine, naphthyridine, quinoxaline,quinazoline, cinnoline, benzoxazole, benzothiazole, benzimidazole,chromene, benzoxepine, benzoxazepine, benzoxadiazepine, benzothiepine,benzothiazepine, benzothiadiazepine, benzoazepine, benzodiazepine,benzofurazan, benzothiadiazole, benzotriazole, carbazole, β-carboline,acridine, phenazine, dibenzofuran, xanthene, dibenzothiophene,phenothiazine, phenoxazine, phenoxathiin, thianthrene, phenanthridine,phenanthroline, perimidine, aziridine, azetidine, pyrroline,pyrrolidine, imidazoline, imidazolidine, triazoline, triazolidine,tetrazoline, tetrazolidine, pyrazoline, pyrazolidine, dihydropyridine,tetrahydropyridine, piperidine, dihydropyrazine, tetrahydropyrazine,piperazine, dihydropyrimidine, tetrahydropyrimidine, perhydropyrimidine,dihydropyridazine, tetrahydropyridazine, perhydropyridazine,dihydroazepine, tetrahydroazepine, perhydroazepine, dihydrodiazepine,tetrahydrodiazepine, perhydrodiazepine, oxirane, oxetane, dihydrofuran,tetrahydrofuran, dihydropyran, tetrahydropyran, dihydrooxepine,tetrahydrooxepine, perhydrooxepine, thiirane, thietane,dihydrothiophene, tetrahydrothiophene, dihydrothiopyran,tetrahydrothiopyran, dihydrothiepine, tetrahydrothiepine,perhydrothiepine, dihydrooxazole, tetrahydrooxazole (oxazolidine),dihydroisoxazole, tetrahydroisoxazole (isoxazolidine), dihydrothiazole,tetrahydrothiazole (thiazolidine), dihydroisothiazole,tetrahydroisothiazole (isothiazolidine), dihydrofurazan,tetrahydrofurazan, dihydrooxadiazole, tetrahydrooxadiazole(oxadiazolidine), dihydrooxazine, tetrahydrooxazine, dihydrooxadiazine,tetrahydrooxadiazine, dihydrooxazepine, tetrahydrooxazepine,perhydrooxazepine, dihydrooxadiazepine, tetrahydrooxadiazepine,perhydrooxadiazepine, dihydrothiadiazole, tetrahydrothiadiazole(thiadiazolidine), dihydrothiazine, tetrahydrothiazine,dihydrothiadiazine, tetrahydrothiadiazine, dihydrothiazepine,tetrahydrothiazepine, perhydrothiazepine, dihydrothiadiazepine,tetrahydrothiadiazepine, perhydrothiadiazepine, morpholine,thiomorpholine, oxathiane, indoline, isoindoline, dihydrobenzofuran,perhydrobenzofuran, dihydroisobenzofuran, perhydroisobenzofuran,dihydrobenzothiophene, perhydrobenzothiophene, dihydroisobenzothiophene,perhydroisobenzothiophene, dihydroindazole, perhydroindazole,dihydroquinoline, tetrahydroquinoline, perhydroquinoline,dihydroisoquinoline, tetrahydroisoquinoline, perhydroisoquinoline,dihydrophthalazine, tetrahydrophthalazine, perhydrophthalazine,dihydronaphthyridine, tetrahydronaphthyridine, perhydronaphthyridine,dihydroquinoxaline, tetrahydroquinoxaline, perhydroquinoxaline,dihydroquinazoline, tetrahydroquinazoline, perhydroquinazoline,dihydrocinnoline, tetrahydrocinnoline, perhydrocinnoline, benzoxathiane,dihydrobenzoxazine, dihydrobenzothiazine, pyrazinomorpholine,dihydrobenzoxazole, perhydrobenzoxazole, dihydrobenzothiazole,perhydrobenzothiazole, dihydrobenzimidazole, perhydrobenzimidazole,dihydrobenzazepine, tetrahydro benzoazepin, dihydrobenzodiazepine,tetrahydrobenzodiazepine, benzodioxepane, dihydrobenzoxazepine,tetrahydrobenzoxazepine, dihydrocarbazole, tetrahydrocarbazole,perhydrocarbazole, dihydroacridine, tetrahydroacridine,perhydroacridine, dihydrodibenzofuran, dihydrodibenzothiophene,tetrahydrodibenzofuran, tetrahydrodibenzothiophene,perhydrodibenzofuran, perhydrodibenzothiophene, dioxolane, dioxane,dithiolane, dithiane, dioxaindane, benzodioxane, chroman,benzodithiolane, benzodithiane, 6,7-dihydro-5H-cyclopenta[b]pyrazine,5H-cyclopenta[b]pyrazine, imidazo[2,1-b][1,3]thiazole,pyrido[2,3-b]pyrazine, pyrido[3,4-b]pyrazine,[1,3]thiazolo[4,5-b]pyrazine, thieno[2,3-b]pyrazine,3,4-dihydro-2H-pyrazino[2,3-b][1,4]oxazine,6,7-dihydro-5H-cyclopenta[b]pyrazine, imidazo[1,2-a]pyrazine,6,7-dihydro-5H-cyclopenta[b]pyridine, furo[3,2-b]pyridine,pyrido[2,3-d]pyrimidine, 2,3-dihydro-1H-pyrrolo[2,3-b]pyridine,5,6,7,8-tetrahydro-1,6-naphthylidine,6,7,8,9-tetrahydro-5H-pyrido[2,3-d]azepine,3,4-dihydro-2H-pyrano[3,2-c]pyridine, 2,3-dihydrofuro[3,2-c]pyridine,hexahydro-1H-pyrrolidine, octahydrocyclopenta[c]pyrrole,octahydrocyclopenta[b]pyrrole, octahydropyrrolo[3,2-b]pyrrole,octahydropyrrolo[3,4-c]pyrrole, hexahydro-2H-furo[3,2-b]pyrrole,hexahydro-2H-thieno[3,2-b]pyrrole, decahydroquinoline,decahydro-2,6-naphthylidine, octahydro-2H-quinolidine,octahydro-1H-pyrido[1,2-c]pyrimidine, octahydro-2H-1,4-benzooxazine,decahydro-1,5-naphthylidine, octahydro-1H-pyrrolo[3,4-b]pyridine, andoctahydro-1H-pyrrolo[3,4-c]pyridine rings; examples of the “spiro-boundpolycyclic heterocyclic ring” include azaspiro[4.4]nonane,oxazaspiro[4.4]nonane, dioxaspiro[4.4]nonane, azaspiro[4.5]decane,thiaspiro[4.5]decane, dithiaspiro[4.5]decane, dioxaspiro[4.5]decane,oxazaspiro[4.5]decane, azaspiro[5.5]undecane, oxaspiro[5.5]undecane,dioxaspiro[5.5]undecane, 2,7-diazaspiro[3.5]nonane,2,8-diazaspiro[4.5]decane, 2,7-diazaspiro[4.5]decane,2,9-diazaspiro[5.5]undecane, 2,8-diazaspiro[5.5]undecane,3,9-diazaspiro[5.5]undecane, 2,7-diazaspiro[4.4]nonane,1,2-dihydrospiro[indole-3,4′-piperidine],2,3-dihydro-1H-spiro[isoquinoline-4,4′-piperidine],1′,4′-dihydro-2′H-spiro[piperidine-4,3′-quinoline],2′,3′-dihydro-1′H-spiro[piperidine-4,4′-quinoline],8-azaspiro[4.5]decane, 7-azaspiro[4.5]decane, 3-azaspiro[5.5]undecane,2-azaspiro[5.5]undecane, 1-oxa-4,8-diazaspiro[5.5]undecane,1-oxa-4,9-diazaspiro[5.5]undecane,3,4-dihydrospiro[chromene-2,4′-piperidine], 2-azaspiro[4.4]nonane,7-azaspiro[3.5]nonane, 2,3-dihydrospiro[indene-1,4′-piperidine],3,4-dihydro-2H-spiro[naphthalene-1,4′-piperidine],3,4-dihydro-1H-spiro[naphthalene-2,4′-piperidine],2-azaspiro[4.5]decane, 2-azaspiro[3.5]nonane,1′,2′-dihydrospiro[cyclohexane-1,3′-indole],2′,3′-dihydro-1′H-spiro[cyclohexane-1,4′-isoquinoline],1′,4′-dihydro-2′H-spiro[cyclohexane-1,3′-quinoline],1,6-diazaspiro[3.4]octane, 1,5-diazaspiro[3.4]octane,1,7-diazaspiro[3.5]nonane, 1,6-diazaspiro[3.5]nonane,1,5-diazaspiro[3.5]nonane, 1,7-diazaspiro[4.4]nonane,1,6-diazaspiro[4.4]nonane, 1,8-diazaspiro[4.5]decane,1,7-diazaspiro[4.5]decane, 2,6-diazaspiro[3.4]octane,1,6-diazaspiro[4.5]decane, 2,6-diazaspiro[3.5]nonane,1,9-diazaspiro[5.5]undecane, 1,8-diazaspiro[5.5]undecane,6-azaspiro[3.5]nonane, 6-azaspiro[3.4]octane, 2-azaspiro[3.4]octane,1,7-diazaspiro[5.5]undecane, 1,4,9-triazaspiro[5.5]undecane,1,3,8-triazaspiro[4.5]decane, 1-thia-4,9-diazaspiro[5.5]undecane, and1-thia-4,8-diazaspiro[5.5]undecane rings; and examples of the “bridgedpolycyclic heterocyclic ring” include azabicyclo[2.2.1]heptane,oxabicyclo[2.2.1]heptane, azabicyclo[3.1.1]heptane,azabicyclo[3.2.1]octane, oxabicyclo[3.2.1]octane,azabicyclo[2.2.2]octane, diazabicyclo[2.2.2]octane,1-azatricyclo[3.3.1.1^(3,7)]decane, 3-azabicyclo[3.3.1]nonane, and3,7-diazabicyclo[3.3.1]nonane rings. Among these, examples of the “3- to15-membered monocyclic or polycyclic aromatic heterocyclic ring having,as a hetero atom, 1 to 4 nitrogen atom(s), 1 to 2 oxygen atom(s) and/or1 to 2 sulfur atom(s)” include pyrrole, imidazole, triazole, tetrazole,pyrazole, pyridine, pyrazine, pyrimidine, pyridazine, furan, thiophene,oxazole, isoxazole, thiazole, isothiazole, furazan, oxadiazole,thiadiazole, indole, isoindole, benzofuran, isobenzofuran,benzothiophene, isobenzothiophene, indazole, quinoline, isoquinoline,purine, phthalazine, pteridine, naphthylidine, quinoxaline, quinazoline,cinnoline, benzoxazole, benzothiazole, benzimidazole, benzofurazan,benzothiadiazole, benzotriazole, carbazole, β-carboline, acridine,phenazine, dibenzofuran, dibenzothiophene, phenanthridine,phenanthroline, perimidine rings.

In the present specification, “aliphatic hydrocarbon group” includes,for example, “linear or branched aliphatic hydrocarbon group”. Examplesof the “linear or branched aliphatic hydrocarbon group” include“aliphatic hydrocarbon group having 1 to 8 carbon atom(s)”, and examplesof “aliphatic hydrocarbon group having 1 to 8 carbon atom(s)” includeC1-8 alkyl group, C2-8 alkenyl group, and C2-8 alkynyl group.

Examples of the C1-8 alkyl group include methyl, ethyl, propyl,isopropyl, butyl, sec-butyl, tert-butyl, pentyl, hexyl, heptyl, andoctyl groups, and isomer groups thereof.

Examples of the C2-8 alkenyl group include vinyl, propenyl, butenyl,pentenyl, hexenyl, heptenyl, octenyl, butadienyl, pentadienyl,hexadienyl, heptadienyl, octadienyl, hexatrienyl, heptatrienyl, andoctatrienyl groups, and isomer groups thereof.

Examples of the C2-8 alkynyl group include ethynyl, propynyl, butynyl,pentynyl, hexynyl, heptynyl, octynyl, butadiynyl, pentadiynyl,hexadiynyl, heptadiynyl, octadiynyl, hexatriynyl, heptatriynyl, andoctatriynyl groups, and isomer groups thereof.

In the present specification, “group having a basic group” representedby A¹ and A² is not specifically limited as long as it has a basicgroup. Examples thereof include (1) basic group, (2) aliphatichydrocarbon group which is substituted with a basic group, and also mayhave a substituent(s), and (3) cyclic group which is substituted with abasic group, and also may have a substituent(s).

The “aliphatic hydrocarbon group” in the “aliphatic hydrocarbon groupwhich is substituted with a basic group, and also may have asubstituent(s)” has the same meaning as in the aliphatic hydrocarbongroup.

The “cyclic group” in the “cyclic group which is substituted with abasic group, and also may have a substituent(s)” has the same meaning asin the cyclic group.

The “substituent” in the “aliphatic hydrocarbon group which issubstituted with a basic group, and also may have a substituent(s)” orthe “cyclic group which is substituted with a basic group, and also mayhave a substituent(s)” is not specifically limited as long as it is asubstituent. Examples thereof include the following substituents definedas T.

Examples of T include:

(1) aliphatic hydrocarbon group,

(2) C1-8 alkylidene group (for example, methylidene, ethylidene,propylidene, butylidene, pentylidene, hexylidene, heptylidene, oroctylidene group, and isomer thereof, etc.),

(3) cyclic group,

(4) aliphatic hydrocarbon group substituted with a cyclic group (forexample, cyclopropylmethyl, cyclopentylmethyl, cyclohexylmethyl,phenylmethyl, naphthylmethyl, pyridinylmethyl, cyclopropylethyl,cyclopentylethyl, cyclohexylethyl, phenylethyl, naphthylethyl,pyridinylethyl, cyclopropylpropyl, cyclopentylpropyl, cyclohexylpropyl,phenylmethyl, phenylpropyl, naphthylpropyl, pyridinylpropyl, etc.),(5) hydroxyl group,(6) —O-aliphatic hydrocarbon group (for example, methoxy, ethoxy,propoxy, isopropoxy, butoxy, isobutoxy, tert-butoxy, pentoxy, hexyloxy,heptyloxy, octyloxy, propenyloxy, butenyloxy, pentenyloxy, hexenyloxy,propynyloxy, butynyloxy, pentynyloxy, hexynyloxy, etc.),(7) —O-cyclic group (for example, cyclopropyloxy, cyclopentyloxy,cyclohexyloxy, phenoxy, naphthyloxy, pyridinyloxy, etc.),(8) —O-aliphatic hydrocarbon group-cyclic group (for example,cyclopentylmethoxy, cyclohexylmethoxy, phenylmethoxy, etc.),(9) mercapto group,(10) —S-aliphatic hydrocarbon group (for example, methylthio, ethylthio,propylthio, isopropylthio, butylthio, isobutylthio, tert-butylthio,pentylthio, hexylthio, heptylthio, octylthio, propenylthio, butenylthio,pentenylthio, hexenylthio, propynylthio, butynylthio, pentynylthio,hexynylthio, etc.),(11) —S-cyclic group (for example, cyclopropylthio, cyclopentylthio,cyclohexylthio, phenylthio, naphthylthio, pyridinylthio, etc.),(12) —S-aliphatic hydrocarbon group-cyclic group (for example,cyclopentylmethylthio, cyclohexylmethylthio, phenylmethylthio, etc.),(13) —S(O)-aliphatic hydrocarbon group (for example, methylsulfinyl,ethylsulfinyl, propylsulfinyl, isopropylsulfinyl, butylsulfinyl,isobutylsulfinyl, tert-butylsulfinyl, pentylsulfinyl, hexylsulfinyl,heptylsulfinyl, octylsulfinyl, propenylsulfinyl, butenylsulfinyl,pentenylsulfinyl, hexenylsulfinyl, propynylsulfinyl, butynylsulfinyl,pentynylsulfinyl, hexynylsulfinyl, etc.),(14) —S(O)-cyclic group (for example, cyclopropylsulfinyl,cyclopentylsulfinyl, cyclohexylsulfinyl, phenylsulfinyl,naphthylsulfinyl, pyridinylsulfinyl, etc.),(15) —S(O)-aliphatic hydrocarbon group-cyclic group (for example,cyclopentylmethylsulfinyl, cyclohexylmethylsulfinyl,phenylmethylsulfinyl, etc.),(16) —SO₂-aliphatic hydrocarbon group (for example, methylsulfonyl,ethylsulfonyl, propylsulfonyl, isopropylsulfonyl, butylsulfonyl,isobutylsulfonyl, tert-butylsulfonyl, pentylsulfonyl, hexylsulfonyl,heptylsulfonyl, octylsulfonyl, propenylsulfonyl, butenylsulfonyl,pentenylsulfonyl, hexenylsulfonyl, propynylsulfonyl, butynylsulfonyl,pentynylsulfonyl, hexynylsulfonyl, etc.),(17) —SO₂-cyclic group (for example, cyclopropylsulfonyl,cyclopentylsulfonyl, cyclohexylsulfonyl, phenylsulfonyl,naphthylsulfonyl, pyridinylsulfonyl, etc.),(18) —SO₂-aliphatic hydrocarbon group-cyclic group (for example,cyclopentylmethylsulfonyl, cyclohexylmethylsulfonyl,phenylmethylsulfonyl, etc.),(19) —O—CO-aliphatic hydrocarbon group (for example, methanoyloxy,ethanoyloxy, propanoyloxy, isopropanoyloxy, butanoyloxy, isobutanoyloxy,tert-butanoyloxy, pentanoyloxy, hexanoyloxy, heptanoyloxy, octanoyloxy,propenoyloxy, butenoyloxy, pentenoyloxy, hexenoyloxy, propynoyloxy,butynoyloxy, pentynoyloxy, hexynoyloxy, etc.),(20) —O—CO-cyclic group (for example, cyclopropylcarbonyloxy,cyclopentylcarbonyloxy, cyclohexylcarbonyloxy, phenylcarbonyloxy,naphthylcarbonyloxy, pyridinylcarbonyloxy, etc.),(21) —O—CO-aliphatic hydrocarbon group-cyclic group (for example,cyclopentylmethanoyloxy, cyclohexylmethanoyloxy, phenylmethanoyloxy,etc.),(22) —CO-aliphatic hydrocarbon group (for example, methanoyl, ethanoyl,propanoyl, isopropanoyl, butanoyl, isobutanoyl, tert-butanoyl,pentanoyl, hexanoyl, heptanoyl, octanoyl, propenoyl, butenoyl,pentenoyl, hexenoyl, propynoyl, butynoyl, pentynoyl, hexynoyl, etc.),(23) —CO-cyclic group (for example, cyclopropylcarbonyl,cyclopentylcarbonyl, cyclohexylcarbonyl, phenylcarbonyl,naphthylcarbonyl, pyridinylcarbonyl, etc.),(24) —CO-aliphatic hydrocarbon group-cyclic group (for example,cyclopentylmethanoyl, cyclohexylmethanoyl, phenylmethanoyl, etc.),(25) oxo group,(26) thioxo group,(27) sulfino group,(28) sulfo group,(29) amino group,(30) mono- or di-substituted amino group (“substituent” in “mono- ordi-substituted amino group” herein includes, for example, (1) aliphatichydrocarbon group, (2) cyclic group, and (3) aliphatic hydrocarbon groupsubstituted with a cyclic group, and examples thereof includemethylamino, ethylamino, propylamino, isopropylamino, butylamino,isobutylamino, tert-butylamino, pentylamino, hexylamino, heptylamino,octylamino, dimethylamino, diethylamino, dipropylamino, dibutylamino,dipentylamino, dihexylamino, diheptylamino, dioctylamino,N-methyl-N-ethylamino, cyclopropylamino, cyclopentylamino,cyclohexylamino, phenylamino, diphenylamino, dibenzylamino,N-phenyl-N-methylamino, N-phenyl-N-ethylamino, N-benzyl-N-methylamino,N-benzyl-N-ethylamino, N-cyclohexyl-N-propylamino, etc.),(31) sulfamoyl group,(32) mono- or di-substituted sulfamoyl group (“substituent” in “mono- ordi-substituted sulfamoyl group” include, for example, (1) aliphatichydrocarbon group, (2) cyclic group, and (3) aliphatic hydrocarbon groupsubstituted with a cyclic group, and examples thereof includeN-methylsulfamoyl, N-ethylsulfamoyl, N-propylsulfamoyl,N-isopropylsulfamoyl, N-butylsulfamoyl, N-isobutylsulfamoyl,N-(tert-butyl)sulfamoyl, N-pentylsulfamoyl, N-hexylsulfamoyl,N-heptylsulfamoyl, N-octylsulfamoyl, N,N-dimethylsulfamoyl,N,N-diethylsulfamoyl, N,N-dipropylsulfamoyl, N,N-dibutylsulfamoyl,N,N-dipentylsulfamoyl, N,N-dihexylsulfamoyl, N-methyl-N-ethylsulfamoyl,N-cyclopropylsulfamoyl, N-cyclopentylsulfamoyl, N-cyclohexylsulfamoyl,N-phenylsulfamoyl, N,N-diphenylsulfamoyl, N,N-dibenzylsulfamoyl,N-phenyl-N-methylsulfamoyl, N-phenyl-N-ethylsulfamoyl,N-benzyl-N-methylsulfamoyl, N-benzyl-N-ethylsulfamoyl,N-cyclohexyl-N-propylamino, etc.),(33) carboxy group,(34) —COO-aliphatic hydrocarbon group (for example, methoxycarbonyl,ethoxycarbonyl, propoxycarbonyl, isopropoxycarbonyl, butoxycarbonyl,isobutoxycarbonyl, tert-butoxycarbonyl, pentoxycarbonyl,hexyloxycarbonyl, heptyloxycarbonyl, octyloxycarbonyl,propenyloxycarbonyl, butenyloxycarbonyl, pentenyloxycarbonyl,hexenyloxycarbonyl, propynyloxycarbonyl, butynyloxycarbonyl,pentynyloxycarbonyl, hexynyloxycarbonyl, etc.),(35) —COO-cyclic group (for example, cyclopropyloxycarbonyl,cyclopentyloxycarbonyl, cyclohexyloxycarbonyl, phenoxycarbonyl,naphthyloxycarbonyl, pyridinyloxycarbonyl, etc.),(36) —COO-aliphatic hydrocarbon group-cyclic group (for example,cyclopentylmethoxycarbonyl, cyclohexylmethoxycarbonyl,phenylmethoxycarbonyl, etc.),(37) carbamoyl group,(38) mono- or di-substituted carbamoyl group (“substituent” in “mono- ordi-substituted carbamoyl group” herein includes, for example, (1)aliphatic hydrocarbon group, (2) cyclic group, and (3) aliphatichydrocarbon group substituted with a cyclic group, and examples thereofinclude N-methylcarbamoyl, N-ethylcarbamoyl, N-propylcarbamoyl,N-isopropylcarbamoyl, N-butylcarbamoyl, N-isobutylcarbamoyl,N-(tert-butyl)carbamoyl, N-pentylcarbamoyl, N-hexylcarbamoyl,N-heptylcarbamoyl, N-octylcarbamoyl, N-cyclopropylcarbamoyl,N-cyclopentylcarbamoyl, N-cyclohexylcarbamoyl, N-phenylcarbamoyl,N,N-dimethylcarbamoyl, N,N-diethylcarbamoyl, N,N-dipropylcarbamoyl,N,N-dibutylcarbamoyl, N,N-dipentylcarbamoyl, N,N-dihexylcarbamoyl,N-methyl-N-ethylcarbamoyl, N,N-diphenylcarbamoyl, N,N-dibenzylcarbamoyl,N-phenyl-N-methylcarbamoyl, N-phenyl-N-ethylcarbamoyl,N-benzyl-N-methylcarbamoyl, N-benzyl-N-ethylcarbamoyl, etc.),(39) —NH—CO-aliphatic hydrocarbon group (for example, methanoylamino,ethanoylamino, propanoylamino, isopropanoylamino, butanoylamino,isobutanoylamino, tert-butanoylamino, pentanoylamino, hexanoylamino,heptanoylamino, octanoylamino, propenoylamino, butenoylamino,pentenoylamino, hexenoylamino, propynoylamino, butynoylamino,pentynoylamino, hexynoylamino, etc.),(40) —NH—CO-cyclic group (for example, cyclopropylcarbonylamino,cyclopentylcarbonylamino, cyclohexylcarbonylamino, phenylcarbonylamino,naphthylcarbonylamino, pyridinylcarbonylamino, etc.),(41) —NH—CO-aliphatic hydrocarbon group-cyclic group (for example,cyclopentylmethanoylamino, cyclohexylmethanoylamino,phenylmethanoylamino, etc.),(42) —NH—SO₂-aliphatic hydrocarbon group (for example,methylsulfonylamino, ethylsulfonylamino, propylsulfonylamino,isopropylsulfonylamino, butylsulfonylamino, isobutylsulfonylamino,tert-butylsulfonylamino, pentylsulfonylamino, hexylsulfonylamino,heptylsulfonylamino, octylsulfonylamino, propenylsulfonylamino,butenylsulfonylamino, pentenylsulfonylamino, hexenylsulfonylamino,propynylsulfonylamino, butynylsulfonylamino, pentynylsulfonylamino,hexynylsulfonyl, etc.),(43) —NH—SO₂-cyclic group (for example, cyclopropylsulfonylamino,cyclopentylsulfonylamino, cyclohexylsulfonylamino, phenylsulfonylamino,naphthylsulfonylamino, pyridinylsulfonyl, etc.),(44) —NH—SO₂-aliphatic hydrocarbon group-cyclic group (for example,cyclopentylmethylsulfonylamino, cyclohexylmethylsulfonylamino,phenylmethylsulfonyl, etc.),(45) cyano group,(46) hydrazino group,(47) nitro group,(48) nitroso group,(49) imino group,(50) mono-substituted imino group (“substituent” in the mono-substitutedimino group includes, for example, (1) aliphatic hydrocarbon group, (2)cyclic group, (3) aliphatic hydrocarbon group substituted with a cyclicgroup, (4) hydroxyl group, (5) —O-aliphatic hydrocarbon group, (6)—O-cyclic group, and (7) —O-aliphatic hydrocarbon group-cyclic group,and examples thereof include methylimino, ethylimino, propylimino,isopropylimino, butylimino, isobutylimino, (tert-butyl)imino,pentylimino, hexylimino, heptylimino, octylimino, cyclopropylimino,cyclopentylimino, cyclohexylimino, phenylimino, benzylimino,hydroxyimino, ethoxyimino, propoxyimino, isopropoxyimino, butoxyimino,cyclopentoxyimino, cyclohexyloxyimino, phenoxyimino, benzyloxyimino,etc.),(51) halogen atom (for example, fluorine atom, chlorine atom, bromineatom, iodine atom, etc.),(52) methyl group substituted with 1 to 3 halogen atom(s) (for example,fluoromethyl, difluoromethyl, trifluoromethyl, trichloromethyl, etc.),and(53) methoxy group substituted with 1 to 3 halogen atom(s) (for example,fluoromethoxy, difluoromethoxy, trifluoromethoxy, trichloromethoxy,etc.). These optional substituents may be substituted on the optionalsubstitutable position in optional substitutable number. The number ofsubstituents is preferably from 1 to 8, and more preferably from 1 to 5.The “aliphatic hydrocarbon group” and the “cyclic group” in T have thesame meanings as described above.

The “basic group” in the “group having a basic group” is notspecifically limited as long as it has a basic nitrogen atom. Examplesthereof include (a) amino group, (b) amidino group, (c) guanidino group,(d) hydrazino group, (e) mono- or di-substituted amino group, (f) mono-,di- or tri-substituted amidino group, (g) mono-, di-, tri- ortetra-substituted guanidino group, (h) mono-, di- or tri-substitutedhydrazino group, and (i) nitrogen-containing heterocyclic ring which mayhave a substituent(s). Examples of the “substituent” in the “mono- ordi-substituted amino group” herein include (1) aliphatic hydrocarbongroup (which has the same meaning as described above), (2) cyclic group(which has the same meaning as described above), (3) aliphatichydrocarbon group substituted with a cyclic group (aliphatic hydrocarbonand cyclic group have the same meanings as described above), (4) cyclicgroup substituted with a substituent(s) (substituent has the samemeaning as in T, and cyclic group has the same meaning as describedabove), (5) aliphatic hydrocarbon group substituted with asubstituent(s) (substituent has the same meaning as in T, and aliphatichydrocarbon has the same meaning as described above), (6) aliphatichydrocarbon group substituted with a cyclic group substituted with asubstituent(s) (substituent has the same meaning as in T, and aliphatichydrocarbon and cyclic groups have the same meanings as describedabove), and (7) substituent T described above. These optionalsubstituents may be substituted on the optional substitutable positionin optional substitutable number. The number of substituents ispreferably from 1 to 8, and more preferably from 1 to 5. Examplesthereof include methylamino, ethylamino, propylamino, isopropylamino,butylamino, isobutylamino, tert-butylamino, pentylamino, hexylamino,heptylamino, octylamino, dimethylamino, diethylamino, dipropylamino,dibutylamino, dipentylamino, dihexylamino, diheptylamino, dioctylamino,N-methyl-N-ethylamino, cyclopropylamino, cyclopentylamino,cyclohexylamino, phenylamino, diphenylamino, dibenzylamino,N-phenyl-N-methylamino, N-phenyl-N-ethylamino, N-benzyl-N-methylamino,N-benzyl-N-ethylamino, N-benzyl-N-cyclohexylamino,N-cyclohexyl-N-propylamino, N-cyclohexyl-N-(3-hydroxypropyl)amino,N-(4-hydroxycyclohexyl)-N-propylamino,N-(4-hydroxycyclohexyl)-N-(3-hydroxypropyl)amino,N-(4-hydroxycyclohexyl)methyl-N-propylamino, N-cyclohexyl-N-acetylamino,N-(3-methoxypropyl)-N-propylamino, N-(2-carboxyethyl)-N-propylamino,N-(2-ethylpropyl)-N-propylamino, N-cyclohexyl-N-(methylsulfonyl)amino,N-(tetrahydropyran-4-yl)-N-propylamino, andN-(indan-2-yl)-N-propylamino.

Examples of the “substituent” in the “mono-, di- or tri-substitutedamidino group” include (1) aliphatic hydrocarbon group (which has thesame meaning as described above), (2) cyclic group (which has the samemeaning as described above), (3) aliphatic hydrocarbon group substitutedwith a cyclic group (aliphatic hydrocarbon and cyclic groups have thesame meanings as described above). Examples of the “mono-, di- ortri-substituted amidino group” include methylamidino, ethylamidino,propylamidino, isopropylamidino, butylamidino, isobutylamidino,tert-butylamidino, pentylamidino, hexylamidino, heptylamidino,octylamidino, N,N-dimethylamidino, N,N′-dimethylamidino,N,N,N′-trimethylamidino, N,N-diethylamidino, N,N′-diethylamidino,N,N,N′-triethylamidino, N,N-dipropylamidino, N,N′-dipropylamidino,N,N,N′-tripropylamidino, N,N-dibutylamidino, N,N′-dibutylamidino,N,N,N′-tributylamidino, N,N-dipentylamidino, N,N′-dipentylamidino,N,N,N′-tripentylamidino, N,N-dihexylamidino, N,N′-dihexylamidino,N,N,N′-trihexylamidino, N,N-diheptylamidino, N,N′-diheptylamidino,N,N,N′-triheptylamidino, N,N-dioctylamidino, N,N′-dioctylamidino,N,N,N′-trioctylamidino, N-methyl-N-ethylamidino,N-methyl-N′-ethylamidino, cyclopropylamidino, cyclopentylamidino,cyclohexylamidino, phenylamidino, N,N-diphenylamidino,N,N′-diphenylamidino, N,N,N′-triphenylamidino, N,N-dibenzylamidino,N,N′-dibenzylamidino, N,N,N′-tribenzylamidino,N-phenyl-N′-methylamidino, N-phenyl-N′-ethylamidino,N-benzyl-N-methylamidino, and N-benzyl-N-ethylamidino.

Examples of the “substituent” in the “mono-, di-, tri- ortetra-substituted guanidino group” include (1) aliphatic hydrocarbongroup (which has the same meaning as described above), (2) cyclic group(which has the same meaning as described above), and (3) aliphatichydrocarbon group substituted with a cyclic group (aliphatic hydrocarbonand cyclic have the same meanings as described above). Examples of the“mono-, di-, tri- or tetra-substituted guanidino group” include, forexample, methylguanidino, ethylguanidino, propylguanidino,isopropylguanidino, butylguanidino, isobutylguanidino,tert-butylguanidino, pentylguanidino, hexylguanidino, heptylguanidino,octylguanidino, N,N-dimethylguanidino, N,N′-dimethylguanidino,N,N,N′-trimethylguanidino, N,N,N′,N″-tetramethylguanidino,N,N-diethylguanidino, N,N′-diethylguanidino, N,N,N′-triethylguanidino,N,N,N′,N″-tetraethylguanidino, N,N-dipropylguanidino,N,N′-dipropylguanidino, N,N,N′-tripropylguanidino,N,N,N′,N″-tetrapropylguanidino, N,N-dibutylguanidino,N,N′-dibutylguanidino, N,N,N′-tributylguanidino,N,N,N′,N″-tetrabutylguanidino, N,N-dipentylguanidino,N,N′-dipentylguanidino, N,N,N′-tripentylguanidino,N,N,N′,N″-tetrapentylguanidino, N,N-dihexylguanidino,N,N′-dihexylguanidino, N,N,N′-trihexylguanidino,N,N,N′,N″-tetrahexylguanidino, N,N-diheptylguanidino,N,N′-diheptylguanidino, N,N,N′-triheptylguanidino,N,N,N′,N″-tetraheptylguanidino, N,N-dioctylguanidino,N,N′-dioctylguanidino, N,N,N′-trioctylguanidino,N,N,N′,N″-tetraoctylguanidino, N-methyl-N-ethylguanidino,N-methyl-N′-ethylguanidino, cyclopropylguanidino, cyclopentylguanidino,cyclohexylguanidino, phenylguanidino, N,N-diphenylguanidino,N,N′-diphenylguanidino, N,N,N′-triphenylguanidino,N,N,N′,N″-tetraphenylguanidino, N,N-dibenzylguanidino,N,N′-dibenzylguanidino, N,N,N′-tribenzylguanidino,N,N,N′,N″-tetrabenzylguanidino, N-phenyl-N′-methylguanidino,N-phenyl-N′-ethylguanidino, N-benzyl-N-methylguanidino,N-benzyl-N-ethylguanidino and the like.

Examples of the “substituent” in the “mono-, di- or tri-substitutedhydrazino group” include (1) aliphatic hydrocarbon group (which has thesame meaning as described above), (2) cyclic group (which has the samemeaning as described above), and (3) aliphatic hydrocarbon groupsubstituted with a cyclic group (aliphatic hydrocarbon and cyclic groupshave the same meanings as described above). Examples of the “mono-, di-or tri-substituted hydrazino group” include, for example,methylhydrazino, ethylhydrazino, propylhydrazino, isopropylhydrazino,butylhydrazino, isobutylhydrazino, tert-butylhydrazino, pentylhydrazino,hexylhydrazino, heptylhydrazino, octylhydrazino, N,N-dimethylhydrazino,N,N′-dimethylhydrazino, N,N,N′-trimethylhydrazino, N,N-diethylhydrazino,N,N′-diethylhydrazino, N,N,N′-triethylhydrazino, N,N-dipropylhydrazino,N,N′-dipropylhydrazino, N,N,N′-tripropylhydrazino, N,N-dibutylhydrazino,N,N′-dibutylhydrazino, N,N,N′-tributylhydrazino, N,N-dipentylhydrazino,N,N′-dipentylhydrazino, N,N,N′-tripentylhydrazino, N,N-dihexylhydrazino,N,N′-dihexylhydrazino, N,N,N′-trihexylhydrazino, N,N-diheptylhydrazino,N,N′-diheptylhydrazino, N,N,N′-triheptylhydrazino, N,N-dioctylhydrazino,N,N′-dioctylhydrazino, N,N,N′-trioctylhydrazino,N-methyl-N-ethylhydrazino, N-methyl-N′-ethylhydrazino,cyclopropylhydrazino, cyclopentylhydrazino, cyclohexylhydrazino,phenylhydrazino, N,N-diphenylhydrazino, N,N′-diphenylhydrazino,N,N,N′-triphenylhydrazino, N,N-dibenzylhydrazino,N,N′-dibenzylhydrazino, N,N,N′-tribenzylhydrazino,N-phenyl-N′-methylhydrazino, N-phenyl-N′-ethylhydrazino,N-benzyl-N-methylhydrazino, N-benzyl-N-ethylhydrazino and the like.

Examples of the “nitrogen-containing heterocyclic ring” in the“nitrogen-containing heterocyclic ring which may have a substituent(s)”include, for example, pyrrole, imidazole, triazole, pyrazole, pyridine,pyrazine, pyrimidine, pyridazine, azepine, diazepine, oxazole, thiazole,isoxazole, isothiazole, indole, isoindole, quinoline, isoquinoline,benzoxazole, benzothiazole, benzimidazole, aziridine, azetidine,pyrrolidine, piperidine, piperazine, morpholine, thiomorpholine,perhydroazepine, perhydrodiazepine, indoline, isoindoline, quinazoline,tetrahydroquinoline, perhydroquinoline, tetrahydroisoquinoline,perhydroisoquinoline, tetrahydronaphthyridine, quinoxaline,tetrahydroquinoxaline, dihydrobenzimidazole, perhydrobenzimidazole,carbazole, tetrahydrocarbazole, azabicyclo[3.2.1]octane, quinuclidine,2,8-diazaspiro[4.5]decane, 1,4,9-triazaspiro[5.5]undecane,3,9-diazaspiro[5.5]undecane, 2,9-diazaspiro[5.5]undecane,1,6-diazaspiro[3.4]octane, 1,5-diazaspiro[3.4]octane,1,7-diazaspiro[3.5]nonane, 1,6-diazaspiro[3.5]nonane,1,5-diazaspiro[3.5]nonane, 1,7-diazaspiro[4.4]nonane,1,6-diazaspiro[4.4]nonane, 1,8-diazaspiro[4.5]decane,1,7-diazaspiro[4.5]decane, 2,6-diazaspiro[3.4]octane,1,6-diazaspiro[4.5]decane, 2,6-diazaspiro[3.5]nonane,1,9-diazaspiro[5.5]undecane, 1,8-diazaspiro[5.5]undecane,6-azaspiro[3.5]nonane, 6-azaspiro[3.4]octane, 2-azaspiro[3.4]octane,1,7-diazaspiro[5.5]undecane, 1,4,9-triazaspiro[5.5]undecane,1,3,8-triazaspiro[4.5]decane, 1-thia-4,9-diazaspiro[5.5]undecane,1-thia-4,8-diazaspiro[5.5]undecane ring and the like.

The “substituent” in the “nitrogen-containing heterocyclic ring whichmay have a substituent(s)” has the same meaning as in above described T.These optional substituent(s) may be substituted on the optionalsubstitutable position in optional substitutable number. The number ofsubstituents is preferably from 1 to 8, and more preferably from 1 to 5.

In the present specification, “nitrogen-containing heterocyclic ringwhich may have a substituent(s)” represented by ring A^(1A) and ringA^(2A) has the same meaning as the “nitrogen-containing heterocyclicring which may have a substituent(s)” in A¹ and A².

In the present specification, examples of the “substituent” of the“imidazole or benzimidazole which may have a substituent” represented byring A^(1B) and ring A^(2B) include those exemplified as for T in A¹ andA², and these optional substituents may be substituted on thesubstitutable position and the number of substituents is from 1 to 5,and preferably from 1 to 2.

In the present specification, “spacer having a main chain of 1 to 4atom(s)” represented by B¹ and B², and “spacer having a main chain of 1to 4 atom(s)” represented by L mean the space wherein 1 to 4 atom(s) ofthe main chain are arranged in a line. “Number of atoms of main chain”is counted so that the number of atoms of the main chain is minimized.For example, it is counted that the number of atoms of1,2-cyclopentylene is 2 and the number of atoms of 1,3-cyclopentylene is3. Examples of the “spacer having a main chain of 1 to 4 atom(s)”include divalent group composed of 1 to 4 groups selected optionallyfrom —O—, —S—, —CO—, —SO—, —SO₂—, divalent nitrogen atom which may havea substituent, divalent aliphatic hydrocarbon group having 1 to 4 carbonatom(s) which may have a substituent(s), and divalent 3- to 8-memberedmonocyclic cyclic group which may have a substituent(s), wherein 1 to 4atom(s) of the main chain are arranged in a line.

The “divalent nitrogen atom which may have a substituent” represents, inaddition to —NH—, those wherein hydrogen atom in the “—NH—” group areoptionally substituted with (1) aliphatic hydrocarbon group, (2) cyclicgroup, (3) aliphatic hydrocarbon group substituted with a cyclic group,(4) hydroxyl group, (5) —O-aliphatic hydrocarbon group, (6) —O-cyclicgroup, (7) —O-aliphatic hydrocarbon group-cyclic group, (8)—SO₂-aliphatic hydrocarbon group, (9) —SO₂-cyclic group, (10)—SO₂-aliphatic hydrocarbon group-cyclic group, (11) —CO-aliphatichydrocarbon, (12) —CO-cyclic group, (13) —CO-aliphatic hydrocarbongroup-cyclic group, (14) carboxy group, (15) —COO-aliphatic hydrocarbon,(16) —COO-cyclic group, or (17) —COO-aliphatic hydrocarbon group-cyclicgroup. The substituents (1) to (17) are have same meanings as describedabove.

Examples of the “divalent aliphatic hydrocarbon group having 1 to 4carbon atom(s)” in the “divalent aliphatic hydrocarbon group having 1 to4 carbon atom(s) which may have a substituent(s)” include C1-4 alkylenegroup (for example, —CH₂—, —(CH₂)₂—, —(CH₂)₃—, —(CH₂)₄—, etc.), C2-4alkenylene group (for example, —CH═CH—, —CH₂—CH═CH—, —CH═CH—CH₂—,—(CH₂)₂—CH═CH—, —CH═CH—(CH₂)₂—, —CH₂—CH═CH—CH₂—, etc.), and C2-4alkynylene group (for example, —C≡C—, —CH₂—C≡C—, —C≡C—CH₂—,—(CH₂)₂—C≡C—, —C≡C—(CH₂)₂—, —CH₂—C≡C—CH₂—, etc.). Examples of the“substituent” in the “divalent aliphatic hydrocarbon group having 1 to 4carbon atom(s) which may have a substituent(s)” include thoseexemplified as for T in A¹ and A², and these optional substituents maybe substituted on the substitutable position and the number ofsubstituents is from 1 to 5, and preferably from 1 to 2.

Examples of the “divalent 3- to 8-membered monocyclic cyclic group” inthe “divalent 3- to 8-membered monocyclic cyclic group which may have asubstituent(s)” include divalent group which can be obtained byeliminating optional two hydrogen atoms from the “C3-8 monocyclic cyclicgroup”. Examples of the “C3-8 monocyclic cyclic group” herein include“C3-8 monocyclic carbocyclic ring” and “3- to 8-membered monocyclicheterocyclic ring”. The “C3-8 monocyclic carbocyclic ring” includes C3-8monocyclic unsaturated carbocyclic ring, and partially or completelysaturated one thereof. Examples of the “C3-8 monocyclic unsaturatedcarbocyclic ring, and partially or completely saturated one thereof”include cyclopropane, cyclobutane, cyclopentane, cyclohexane,cycloheptane, cyclooctane, cyclopentene, cyclohexene, cycloheptene,cyclooctene, cyclopentadiene, cyclohexadiene, cycloheptadiene,cyclooctadiene, and benzene rings. Among these, the “C3-8 monocyclicaromatic carbocyclic ring” includes, for example, benzene ring.

Examples of the “3- to 8-membered monocyclic heterocyclic ring” include“3- to 8-membered monocyclic heterocyclic ring having, as a hetero atom,1 to 4 nitrogen atom(s), 1 to 2 oxygen atom(s) and/or 1 to 2 sulfuratom(s)”. The 3- to 8-membered monocyclic heterocyclic ring having, as ahetero atom, 1 to 4 nitrogen atom(s), 1 to 2 oxygen atom(s) and/or 1 to2 sulfur atom(s)” herein includes 3- to 8-membered monocyclicunsaturated heterocyclic ring having, as a hetero atom, 1 to 4 nitrogenatom(s), 1 to 2 oxygen atom(s) and/or 1 to 2 sulfur atom(s), andpartially or completely saturated one thereof. Examples of the “3- to8-membered monocyclic unsaturated heterocyclic ring having, as a heteroatom, 1 to 4 nitrogen atom(s), 1 to 2 oxygen atom(s) and/or 1 to 2sulfur atom(s), and partially or completely saturated one thereof”include pyrrole, imidazole, triazole, tetrazole, pyrazole, pyridine,pyrazine, pyrimidine, pyridazine, azepine, diazepine, furan, pyran,oxepine, thiophene, thiopyran, thiepine, oxazole, isoxazole, thiazole,isothiazole, furazan, oxadiazole, oxazine, oxadiazine, oxazepine,oxadiazepine, thiadiazole, thiazine, thiadiazine, thiazepine,thiadiazepine, aziridine, azetidine, pyrroline, pyrrolidine,imidazoline, imidazolidine, triazoline, triazolysine, tetrazoline,tetrazolidine, pyrazoline, pyrazolidine, dihydropyridine,tetrahydropyridine, piperidine, dihydropyrazine, tetrahydropyrazine,piperazine, dihydropyrimidine, tetrahydropyrimidine, perhydropyrimidine,dihydropyridazine, tetrahydropyridazine, perhydropyridazine,dihydroazepine, tetrahydroazepine, perhydroazepine, dihydrodiazepine,tetrahydrodiazepine, perhydrodiazepine, oxirane, oxetane, dihydropyran,tetrahydropyran, dihydropyran, tetrahydropyran, dihydrooxepine,tetrahydrooxepine, perhydrooxepine, thiirane, thietane,dihydrothiophene, tetrahydrothiophene, dihydrothiopyran,tetrahydrothiopyran, dihydrothiepine, tetrahydrothiepine,perhydrothiepine, dihydrooxazole, tetrahydrooxazole (oxazolidine),dihydroisoxazole, tetrahydroisoxazole (isoxazolidine), dihydrothiazole,tetrahydrothiazole (thiazolidine), dihydroisothiazole,tetrahydroisothiazole (isothiazolidine), dihydrofurazan,tetrahydrofurazan, dihydrooxadiazole, tetrahydrooxadiazole(oxadiazolidine), dihydrooxazine, tetrahydrooxazine, dihydrooxadiazine,tetrahydrooxadiazine, dihydrooxazepine, tetrahydrooxazepine,perhydrooxazepine, dihydrooxadiazepine, tetrahydrooxadiazepine,perhydrooxadiazepine, dihydrothiadiazole, tetrahydrothiadiazole(thiadiazolidine), dihydrothiazine, tetrahydrothiazine,dihydrothiadiazine, tetrahydrothiadiazine, dihydrothiazepine,tetrahydrothiazepine, perhydrothiazepine, dihydrothiadiazepine,tetrahydrothiadiazepine, perhydrothiadiazepine, morpholine,thiomorpholine, oxathiane, dioxolane, dioxane, dithiolane, dithianerings and the like. Among these, examples of the “3- to 8-memberedmonocyclic aromatic heterocyclic ring having, as a hetero atom, 1 to 4nitrogen atom(s), 1 to 2 oxygen atom(s) and/or 1 to 2 sulfur atom(s)”include pyrrole, imidazole, triazole, tetrazole, pyrazole, pyridine,pyrazine, pyrimidine, pyridazine, furan, thiophene, oxazole, isoxazole,thiazole, isothiazole, furazan, oxadiazole, thiadiazole rings and thelike. Examples of the “substituent” in the “divalent 3- to 8-memberedmonocyclic cyclic group which may have a substituent(s)” include thoseexemplified as for T in A¹ and A², and these optional substituents maybe substituted on the substitutable position and the number ofsubstituents is from 1 to 8, and preferably from 1 to 5.

In the present specification, “spacer having a main chain of 1 to 10atom(s)” represented by E means the space wherein 1 to 10 atom(s) of themain chain are arranged in a line. “Number of atoms of main chain” iscounted so that the number of atoms of the main chain is minimized. Forexample, it is counted that the number of atoms of

is 4 and the number of atoms of

is 6. Examples of the “spacer having a main chain of 1 to 10 atom(s)”include divalent group composed of 1 to 10 groups selected optionallyfrom —O—, —S—, —CO—, —SO—, —SO₂—, divalent nitrogen atom which may havea substituent, divalent aliphatic hydrocarbon group having 1 to 10carbon atom(s) which may have a substituent(s), and divalent 3- to15-membered monocyclic cyclic group which may have a substituent(s),wherein 1 to 10 atom(s) of the main chain are arranged in a line. The“divalent nitrogen atom which may have a substituent” has the samemeaning as described above. Examples of the “divalent aliphatichydrocarbon group having 1 to 10 carbon atom(s)” in the “divalentaliphatic hydrocarbon group having 1 to 10 carbon atom(s) which may havea substituent(s)” include C1-10 alkylene group (methylene, ethylene,trimethylene, tetramethylene, pentamethylene, hexamethylene,heptamethylene, octamethylene, nonamethylene, decamethylene group, andisomers thereof), C2-10 alkenylene group (ethenylene, propenylene,butenylene, pentenylene, hexenylene, heptenylene, octenylene,nonenylene, decenylene group, and isomers thereof), and C2-10 alkynylenegroup (ethenylene, propynylene, butynylene, pentynylene, hexynylene,heptynylene, octynylene, nonynylene, decynylene group, and isomersthereof). Examples of the “substituent” in the “divalent aliphatichydrocarbon group having 1 to 10 carbon atom(s) which may have asubstituent(s)” include those exemplified as for T in A¹ and A², andthese optional substituents may be substituted on the substitutableposition and the number of substituents is from 1 to 5, and preferablyfrom 1 to 2. Examples of the “divalent 3- to 15-membered cyclic group”in the “divalent 3- to 15-membered cyclic group which may have asubstituent(s)” include divalent group obtained by eliminating optionaltwo hydrogen atoms from the “3- to 15-membered cyclic group”. Examplesof the “3- to 15-membered cyclic group” include C3-15 monocyclic orpolycyclic carbocyclic ring defined above, or 3- to 15-memberedmonocyclic or polycyclic heterocyclic ring having, as a hetero atom, 1to 4 nitrogen atom(s), 1 to 2 oxygen atom(s) and/or 1 to 2 sulfuratom(s). Examples of the “substituent” in the “divalent 3- to15-membered cyclic group which may have a substituent(s)” include thoseexemplified as for T in A¹ and A², and these optional substituents maybe substituted on the substitutable position and the number ofsubstituents is from 1 to 5, and preferably from 1 to 2.

In the present specification, the “divalent 3- to 8-membered monocycliccyclic group which may have a substituent(s)” of the “divalent 3- to8-membered monocyclic cyclic group which may have a substituent(s), ordivalent 9- or 10-membered polycyclic cyclic group which may have asubstituent(s)” represented by ring E^(A) has the same meaning as in the“divalent 3- to 8-membered monocyclic cyclic group which may have asubstituent(s)” in B¹ and B². As the “divalent 9- or 10-memberedpolycyclic cyclic group which may have a substituent(s)” of the“divalent 3- to 8-membered monocyclic cyclic group which may have asubstituent(s), or divalent 9- or 10-membered polycyclic cyclic groupwhich may have a substituent(s)” represented by ring E^(A), a 9- or10-membered cyclic group is selected from among the “divalent 3- to15-membered cyclic group which may have a substituent(s)” in E.

In the present specification, “-(nitrogen atom which may have asubstituent)-” in “-(aliphatic hydrocarbon group having 1 to 3 carbonatom(s) which may have a substituent(s))-(nitrogen atom which may have asubstituent)-” represented by L^(A) and L^(A1) has the same meaning asin the “divalent nitrogen atom which may have a substituent”. Examplesof the “aliphatic hydrocarbon group having 1 to 3 carbon atom(s)” in the“aliphatic hydrocarbon group having 1 to 3 carbon atom(s) which may havea substituent(s)” include C1 to 3 alkylene group (for example,methylene, ethylene, trimethylene, etc.), C2-3 alkenylene group (forexample, ethenylene, propenylene, etc.), and C2-3 alkynylene group (forexample, ethynylene, propynylene, etc.). Examples of the “substituent”in the “aliphatic hydrocarbon group having 1 to 3 carbon atom(s) whichmay have a substituent(s)” include those exemplified as for T in A¹ andA², and these optional substituents may be substituted on thesubstitutable position and the number of substituents is from 1 to 3.

In the present specification, the “divalent aliphatic hydrocarbon grouphaving 1 to 4 carbon atom(s) which may have a substituent(s)”represented by L^(A) and L^(A2) has the same meaning as described above.

In the present specification, examples of the “aliphatic hydrocarbongroup which is substituted with a group having a basic group, and alsomay have a substituent(s)” represented by J⁰ include (1) aliphatichydrocarbon group which is substituted with a basic group, and also mayhave a substituent(s), (2) aliphatic hydrocarbon group which issubstituted with a cyclic group substituted with a basic group, and alsomay have a substituent(s), and (3) aliphatic hydrocarbon group which issubstituted with an aliphatic hydrocarbon group substituted with a basicgroup, and also may have a substituent(s). Examples of the “cyclic groupwhich is substituted with a group having a basic group, and also mayhave a substituent(s)” represented by J⁰ include (1) cyclic group whichis substituted with a basic group, and also may have a substituent(s),(2) cyclic group which is substituted with a cyclic group substitutedwith a basic group, and also may have a substituent(s), and (3) cyclicgroup which is substituted with an aliphatic hydrocarbon groupsubstituted with a basic group, and also may have a substituent(s).Examples of the “spiro-bound cyclic group which may be substituted witha group having a basic group, and also may have a substituent(s)”represented by J⁰ include (1) spiro-bound cyclic group which may besubstituted with a basic group, and also may have a substituent(s), (2)spiro-bound cyclic group which may be substituted with a cyclic groupsubstituted with a basic group, and also may have a substituent(s), and(3) spiro-bound cyclic group which may be substituted with an aliphatichydrocarbon group substituted with a basic group, and also may have asubstituent(s). Examples of the “bridged cyclic group which may besubstituted with a group having a basic group, and also may have asubstituent(s)” represented by J⁰ include (1) bridged cyclic group whichmay be substituted with a basic group, and also may have asubstituent(s), (2) bridged cyclic group which may be substituted with acyclic group substituted with a basic group, and also may have asubstituent(s), and (3) bridged cyclic group which may be substitutedwith an aliphatic hydrocarbon group substituted with a basic group, andalso may have a substituent(s). The “spiro-bound cyclic group” in the“spiro-bound cyclic group which may be substituted with a group having abasic group, and also may have a substituent(s)” has the same meaning asin “spiro-bound polycyclic carbocyclic ring” of “carbocyclic ring” inthe “cyclic group” or “spiro-bound polycyclic heterocyclic ring” of“heterocyclic ring” in the “cyclic group”. The “bridged cyclic group” ofthe “bridged cyclic group which may be substituted with a group having abasic group, and also may have a substituent(s)” has the same meaning asin “bridged polycyclic carbocyclic ring” of “carbocyclic ring” in the“cyclic group” or “bridged polycyclic heterocyclic ring” of“heterocyclic ring”. The “aliphatic hydrocarbon group” and “cyclicgroup” herein have the same meaning as described above. The “basicgroup” herein has the same meaning as in the “basic group” in A¹ and A².The “substituent” herein is not specifically limited. Examples thereofinclude those exemplified as for T in A¹ and A², and these optionalsubstituents may be substituted on the substitutable position and thenumber of substituents is from 1 to 5.

In the present specification, the “spiro-bound heterocyclic ring orbridged heterocyclic ring which has at least one nitrogen atom, and alsomay have an oxygen atom and/or a sulfur atom which may be oxidized” inthe “spiro-bound heterocyclic ring or bridged heterocyclic ring whichmay be substituted with a group having a basic group and also may have asubstituent(s), and also may have at least one nitrogen atom and alsomay have an oxygen atom and/or a sulfur atom which may be oxidized”represented by J⁰ includes spiro-bound heterocyclic ring which has atleast one nitrogen atom, and may also have an oxygen atom and/or asulfur atom which may be oxidized, and bridged heterocyclic ring whichhas at least one nitrogen atom, and also may have an oxygen atom and/ora sulfur atom which may be oxidized. Examples of the “spiro-boundheterocyclic ring which has at least one nitrogen atom, and also mayhave an oxygen atom and/or a sulfur atom” include, for example,azaspiro[4.4]nonane, oxazaspiro[4.4]nonane, azaspiro[4.5]decane,oxazaspiro[4.5]decane, azaspiro[5.5]undecane, 2,7-diazaspiro[3.5]nonane,2,8-diazaspiro[4.5]decane,2,7-diazaspiro[4.5]decane2,9-diazaspiro[5.5]undecane,2,8-diazaspiro[5.5]undecane, 3,9-diazaspiro[5.5]undecane,2,7-diazaspiro[4.4]nonane, 1,2-dihydrospiro[indole-3,4′-piperidine],2,3-dihydro-1H-spiro[isoquinoline-4,4′-piperidine],1′,4′-dihydro-2′H-spiro[piperidine-4,3′-quinoline],2′,3′-dihydro-1′H-spiro[piperidine-4,4′-quinoline],8-azaspiro[4.5]decane, 8-azaspiro[4.5]decane, 7-azaspiro[4.5]decane,3-azaspiro[5.5]undecane, 2-azaspiro[5.5]undecane,1-oxa-4,8-diazaspiro[5.5]undecane, 1-oxa-4,9-diazaspiro[5.5]undecane,3,4-dihydrospiro[chromene-2,4′-piperidine], 2-azaspiro[4.4]nonane,7-azaspiro[3.5]nonane, 2,3-dihydrospiro[indene-1,4′-piperidine],3,4-dihydro-2H-spiro[naphthalene-1,4′-piperidine],3,4-dihydro-1H-spiro[naphthalene-2,4′-piperidine],2-azaspiro[4.5]decane, 2-azaspiro[3.5]nonane,1′,2′-dihydrospiro[cyclohexane-1,3′-indole],2′,3′-dihydro-1′H-spiro[cyclohexane-1,4′-isoquinoline],1′,4′-dihydro-2′H-spiro[cyclohexane-1,3′-quinoline],1,6-diazaspiro[3.4]octane, 1,5-diazaspiro[3.4]octane,1,7-diazaspiro[3.5]nonane, 1,6-diazaspiro[3.5]nonane,1,5-diazaspiro[3.5]nonane, 1,7-diazaspiro[4.4]nonane,1,6-diazaspiro[4.4]nonane, 1,8-diazaspiro[4.5]decane,1,7-diazaspiro[4.5]decane, 2,6-diazaspiro[3.4]octane,1,6-diazaspiro[4.5]decane, 2,6-diazaspiro[3.5]nonane,1,9-diazaspiro[5.5]undecane, 1,8-diazaspiro[5.5]undecane,6-azaspiro[3.5]nonane, 6-azaspiro[3.4]octane, 2-azaspiro[3.4]octane,1,7-diazaspiro[5.5]undecane, 1,4,9-triazaspiro[5.5]undecane,1,3,8-triazaspiro[4.5]decane, 1-thia-4,9-diazaspiro[5.5]undecane,1-thia-4,8-diazaspiro[5.5]undecane and the like. Examples of the“bridged heterocyclic ring which has at least one nitrogen atom, andalso may have an oxygen atom and/or a sulfur atom include, for example,azabicyclo[2.2.1]heptane, azabicyclo[3.1.1]heptane,azabicyclo[3.2.1]octane, azabicyclo[2.2.2]octane,diazabicyclo[2.2.2]octane, 1-azatricyclo[3.3.1.1^(3,7)]decane,3-azabicyclo[3.3.1]nonane, 3,7-diazabicyclo[3.3.1]nonane and the like.

In the present specification, the “7- to 15-membered bicyclicspiro-bound heterocyclic ring wherein a ring comprising the spiro-boundheterocyclic ring is a monocycle composed of at least one nitrogen atomand carbon atoms, and/or a monocycle composed of at least one nitrogenatom, one oxygen atom and carbon atoms” represented by J⁰ includes (1)7- to 15-membered bicyclic spiro-bound heterocyclic ring wherein a ringcomprising the spiro-bound heterocyclic ring is two (a) 4- to 8-memberedmonocycles composed of at least one nitrogen atom and carbon atoms, (2)7- to 15-membered bicyclic spiro-bound heterocyclic ring wherein a ringcomprising the spiro-bound heterocyclic ring is (a) 4- to 8-memberedmonocycle composed of at least one nitrogen atom and carbon atoms, and(b) 4- to 8-membered monocycle composed of at least one nitrogen atom,one oxygen atom and carbon atoms, and (3) 7- to 15-membered bicyclicspiro-bound heterocyclic ring wherein a ring comprising the spiro-boundheterocyclic ring is two (b) monocycles composed of at least onenitrogen atom, one oxygen atom and carbon atoms.

“(1) 7- to 15-membered bicyclic spiro-bound heterocyclic ring wherein aring comprising the spiro-bound heterocyclic ring is two (a) 4- to8-membered monocycles composed of at least one nitrogen atom and carbonatoms” means that two rings selected optionally from “(a) 4- to8-membered monocycle composed of at least one nitrogen atom and carbonatoms” share one carbon atom. The shared carbon atom may be any carbonatom as long as it is a carbon atom constituting the monocycle.

“(2) 7- to 15-membered bicyclic spiro-bound heterocyclic ring wherein aring comprising the spiro-bound heterocyclic ring is (a) 4- to8-membered monocycle composed of at least one nitrogen atom and carbonatoms, and (b) 4- to 8-membered monocycle composed of at least onenitrogen atom, one oxygen atom and carbon atoms” means that one ringselected optionally from “(a) 4- to 8-membered monocycle composed of atleast one nitrogen atom and carbon atoms” and one ring selectedoptionally from “(b) 4- to 8-membered monocycle composed of at least onenitrogen atom, one oxygen atom and carbon atoms” share one carbon atom.The shared carbon atom may be any carbon atom as long as it is a carbonatom constituting the monocycle.

“(3) 7- to 15-membered bicyclic spiro-bound heterocyclic ring wherein aring comprising the spiro-bound heterocyclic ring is two (b) monocyclescomposed of at least one nitrogen atom, one oxygen atom and carbonatoms” means that two rings selected optionally from “(b) monocyclecomposed of at least one nitrogen atom, one oxygen atom and carbonatoms” shares one carbon atom. The shared carbon atom may be any carbonatom as long as it is a carbon atom constituting the monocycle.

Examples of the “4- to 8-membered monocycle composed of at least onenitrogen atom and carbon atoms” include, for example, azetidine,pyrrolidine, piperidine, piperazine, azepane, 1,4-diazepane, azocane,1,4-diazocane, 1,5-diazocane, morpholine and the like.

Examples of the “4- to 8-membered monocycle composed of at least onenitrogen atom, one oxygen atom and carbon atoms” include, for example,1,4-oxazepane, 1,4-oxazocane, 1,5-oxazocane and the like.

Examples of “(1) 7- to 15-membered bicyclic spiro-bound heterocyclicring wherein a ring comprising the spiro-bound heterocyclic ring is two(a) 4- to 8-membered monocycles composed of at least one nitrogen atomand carbon atoms” include, for example, 2,7-diazaspiro[3.5]nonane,2,8-diazaspiro[4.5]decane, 2,7-diazaspiro[4.5]decane,2,9-diazaspiro[5.5]undecane, 2,8-diazaspiro[5.5]undecane,2,6-diazaspiro[3.5]nonane, 3,9-diazaspiro[5.5]undecane,2,7-diazaspiro[4.4]nonane, 1,3,8-triazaspiro[4.5]decane,1,4,9-triazaspiro[5.5]undecane and the like.

Examples of “(2) 7- to 15-membered bicyclic spiro-bound heterocyclicring wherein a ring comprising the spiro-bound heterocyclic ring is (a)4- to 8-membered monocycle composed of at least one nitrogen atom andcarbon atoms, and (b) 4- to 8-membered monocycle composed of at leastone nitrogen atom, one oxygen atom and carbon atoms” include1-oxa-4,9-diazaspiro[5.5]undecane, and1-oxa-4,8-diazaspiro[5.5]undecane.

Examples of “(3) 7- to 15-membered bicyclic spiro-bound heterocyclicring wherein a ring comprising the spiro-bound heterocyclic ring is two(b) monocycles composed of at least one nitrogen atom, one oxygen atomand carbon atoms” include 2,9-dioxa-5,12-diazaspiro[6.6]tridecane.

In the present specification, examples of the “spiro-bound carbocyclicring or bridged carbocyclic ring” in the “spiro-bound carbocyclic ringor bridged carbocyclic ring which is substituted with a group having abasic group, and also may have a substituent(s)” represented by J⁰include “spiro-bound polycyclic carbocyclic ring” or “bridged polycycliccarbocyclic ring” in the “cyclic group”.

The “substituent” in the J⁰ group is not specifically limited. Examplesthereof include those exemplified as for T in A¹ and A², and theseoptional substituents may be substituted on the substitutable positionand the number of substituents is from 1 to 5. Furthermore, the“aliphatic hydrocarbon group”, “cyclic group” and “basic group” in theJ⁰ group have the same meaning as in the “aliphatic hydrocarbon group”,the “cyclic group” and the “basic group” that are described above.

In the present specification, ring J¹ and ring J² represent a ringconstituting a spiro ring.

In the present specification, the “C3-10 monocyclic or bicycliccarbocyclic ring” represented by ring J¹ and ring J^(1a) includes aC3-10 monocyclic or bicyclic unsaturated carbocyclic ring, and partiallyor completely saturated one thereof. Examples thereof include, forexample, benzene, azulene, naphthalene, cyclopropane, cyclobutane,cyclopentane, cyclohexane, cycloheptane, cyclooctane, cyclononane,cyclodecane, cyclohexene, cycloheptene, cyclooctene, cyclopentadiene,cyclohexadiene, cycloheptadiene, cyclooctadiene, pentalene,perhydropentalene, perhydroazulene, indene, perhydroindene, indane,dihydronaphthalene, tetrahydronaphthalene, perhydronaphthalene,heptalene, perhydroheptalene and the like.

In the present specification, the “3- to 10-membered monocyclic orbicyclic heterocyclic ring composed of carbon atom(s), oxygen atom(s)and/or sulfur atom(s) which may be oxidized” represented by ring J¹ andring J^(1a) includes a 3- to 10-membered monocyclic or bicyclicunsaturated heterocyclic ring composed of carbon atom, oxygen atomand/or sulfur atom which may be oxidized, and partially or completelysaturated one thereof. Examples thereof include, for example, furan,pyran, oxepine, thiophene, thiopyran, thiepine, benzofuran,isobenzofuran, benzothiophene, isobenzothiophene, dithianaphthalene,chromene, benzoxepine, benzothiepine, oxirane, oxetane, dihydropyran,tetrahydropyran, dihydropyran, tetrahydropyran, dihydrooxepine,tetrahydrooxepine, perhydrooxepine, thiirane, thietane,dihydrothiophene, tetrahydrothiophene, dihydrothiopyran,tetrahydrothiopyran, dihydrothiepine, tetrahydrothiepine,perhydrothiepine, oxathiane, dihydrobenzofuran, perhydrobenzofuran,dihydroisobenzofuran, perhydroisobenzofuran, dihydrobenzothiophene,perhydrobenzothiophene, dihydroisobenzothiophene,perhydroisobenzothiophene, benzoxathiane, benzodioxepane, dioxolane,dioxane, dithiolane, dithiane, dioxaindane, benzodioxane, chroman,benzodithiolane, benzodithiane and the like.

In the present specification, the “3- to 10-membered monocyclic orbicyclic heterocyclic ring which has at least one nitrogen atom, andalso may have oxygen atom(s) or sulfur atom(s) which may be oxidized”represented by ring J¹ and ring J^(1b) includes a 3- to 10-memberedmonocyclic or bicyclic unsaturated heterocyclic ring which has at leastone nitrogen atom and also may have oxygen atom or sulfur atom which maybe oxidized, and partially or completely saturated one thereof. Examplesthereof include, for example, aziridine, azetidine, pyrroline,pyrrolidine, imidazoline, imidazolidine, triazoline, triazolidine,tetrazoline, tetrazolidine, pyrazoline, pyrazolidine, dihydropyridine,tetrahydropyridine, piperidine, dihydropyrazine, tetrahydropyrazine,piperazine, dihydropyrimidine, tetrahydropyrimidine, perhydropyrimidine,dihydropyridazine, tetrahydropyridazine, perhydropyridazine,dihydroazepine, tetrahydroazepine, perhydroazepine, dihydrodiazepine,tetrahydrodiazepine, perhydrodiazepine, dihydrooxazole,tetrahydrooxazole (oxazolidine), dihydroisoxazole, tetrahydroisoxazole(isoxazolidine), dihydrothiazole, tetrahydrothiazole (thiazolidine),dihydroisothiazole, tetrahydroisothiazole (isothiazolidine),dihydrofurazan, tetrahydrofurazan, dihydrooxadiazole,tetrahydrooxadiazole (oxadiazolidine), dihydrooxazine,tetrahydrooxazine, dihydrooxadiazine, tetrahydrooxadiazine,dihydrooxazepine, tetrahydrooxazepine, perhydrooxazepine,dihydrooxadiazepine, tetrahydrooxadiazepine, perhydrooxadiazepine,dihydrothiadiazole, tetrahydrothiadiazole (thiadiazolidine),dihydrothiazine, tetrahydrothiazine, dihydrothiadiazine,tetrahydrothiadiazine, dihydrothiazepine, tetrahydrothiazepine,perhydrothiazepine, dihydrothiadiazepine, tetrahydrothiadiazepine,perhydrothiadiazepine, morpholine, thiomorpholine, indoline,isoindoline, dihydroindazole, perhydroindazole, dihydroquinoline,tetrahydroquinoline, perhydroquinoline, dihydroisoquinoline,tetrahydroisoquinoline, perhydroisoquinoline, dihydrophthalazine,tetrahydrophthalazine, perhydrophthalazine, dihydronaphthyridine,tetrahydronaphthyridine, perhydronaphthyridine, dihydroquinoxaline,tetrahydroquinoxaline, perhydroquinoxaline, dihydroquinazoline,tetrahydroquinazoline, perhydroquinazoline, dihydrocinnoline,tetrahydrocinnoline, perhydrocinnoline, dihydrobenzoxazine,dihydrobenzothiazine, pyrazinomorpholine, dihydrobenzoxazole,perhydrobenzoxazole, dihydrobenzothiazole, perhydrobenzothiazole,dihydrobenzimidazole, perhydrobenzimidazole, dihydrobenzazepine,tetrahydrobenzazepine, dihydrobenzodiazepine, tetrahydrobenzodiazepine,benzodioxepane, dihydrobenzoxazepine, tetrahydrobenzoxazepine, pyrrole,imidazole, triazole, tetrazole, pyrazole, pyridine, pyrazine,pyrimidine, pyridazine, azepine, diazepine, oxazole, isoxazole,thiazole, isothiazole, furazan, oxadiazole, oxazine, oxadiazine,oxazepine, oxadiazepine, thiadiazole, thiazine, thiadiazine, thiazepine,thiadiazepine, indole, isoindole, indolizine, indazole, quinoline,isoquinoline, quinolizine, purine, phthalazine, pteridine,naphthyridine, quinoxaline, quinazoline, cinnoline, benzoxazole,benzothiazole, benzimidazole, benzoxazepine, benzoxadiazepine,benzothiepine, benzothiazepine, benzothiadiazepine, benzoazepine,benzodiazepine, benzofurazan, benzothiadiazole, benzotriazole and thelike.

The “C3-10 monocyclic or bicyclic carbocyclic ring”, “3- to 10-memberedmonocyclic or bicyclic heterocyclic ring composed of carbon atom(s),oxygen atom(s) and/or sulfur atom(s) which may be oxidized”, or “3- to10-membered monocyclic or bicyclic heterocyclic ring which has at leastone nitrogen atom and also may have oxygen atom(s) or sulfur atom(s)which may be oxidized” in the “C3-10 monocyclic or bicyclic carbocyclicring substituted with a group having a basic group”, “3- to 10-memberedmonocyclic or bicyclic heterocyclic ring composed of carbon atom(s),oxygen atom(s) and/or sulfur atom(s) which may be oxidized, which issubstituted with a group having a basic group”, or “3- to 10-memberedmonocyclic or bicyclic heterocyclic ring which has at least one nitrogenatom and also may have oxygen atom(s) or sulfur atom(s) which may beoxidized, and also may be substituted with a group having a basic group”represented by ring J² has the same meaning as described above. The“C3-10 monocyclic or bicyclic carbocyclic ring” or “3- to 10-memberedmonocyclic or bicyclic heterocyclic ring composed of carbon atom(s),oxygen atom(s) and/or sulfur atom(s) which may be oxidized” in the“C3-10 monocyclic or bicyclic carbocyclic ring substituted with a grouphaving a basic group” or “3- to 10-membered monocyclic or bicyclicheterocyclic ring composed of carbon atom(s), oxygen atom(s) and/orsulfur atom(s) which may be oxidized, which is substituted with a grouphaving a basic group” represented by ring J^(2a) has the same meaning asdescribed above. The “3- to 10-membered monocyclic or bicyclicheterocyclic ring which has at least one nitrogen atom and also may haveoxygen atom(s) or sulfur atom(s) which may be oxidized” in the “3- to10-membered monocyclic or bicyclic heterocyclic ring which has at leastone nitrogen atom and also may have oxygen atom or sulfur atom which maybe oxidized, which may be substituted with a group having a basic group”represented by ring J^(2b) has the same meaning as described above. The“group having a basic group” herein has the same meaning as in the“group having a basic group” in the “spiro-bound cyclic group which maybe substituted with a group having a basic group, and also may have asubstituent(s)”.

In the present specification, the “bridged polycyclic carbocyclic ring”in the “bridged polycyclic carbocyclic ring substituted with a grouphaving a basic group” represented by ring J³ has the same meaning as inthe above-described “bridged polycyclic carbocyclic ring” in the “cyclicgroup”.

In the present specification, examples of the “bridged polycyclicheterocyclic ring composed of carbon atom(s), oxygen atom(s) and/orsulfur atom(s) which may be oxidized” of the “bridged polycyclicheterocyclic ring composed of carbon atom(s), oxygen atom(s) and/orsulfur atom(s) which may be oxidized, which is substituted with a grouphaving a basic group” represented by ring J³ include, for example,oxabicyclo[2.2.1]heptane oxabicyclo[3.2.1]octane and the like.

In the present specification, examples of the “bridged polycyclicheterocyclic ring which has at least one nitrogen atom and also may havean oxygen atom(s) and/or a sulfur atom(s) which may be oxidized” of the“bridged polycyclic heterocyclic ring which may have at least onenitrogen atom and also may have an oxygen atom(s) and/or a sulfuratom(s) which may be oxidized, which may be substituted with a grouphaving a basic group” represented by ring J³ include, for example,azabicyclo[2.2.1]heptane, oxabicyclo[2.2.1]heptane,azabicyclo[3.1.1]heptane, azabicyclo[3.2.1]octane,oxabicyclo[3.2.1]octane, azabicyclo[2.2.2]octane,diazabicyclo[2.2.2]octane, 1-azatricyclo[3.3.1.1^(3,7)]decane,3-azabicyclo[3.3.1]nonane, 3,7-diazabicyclo[3.3.1]nonane and the like.

The “group having a basic group” in ring J³ has the same meaning as inthe “group having a basic group” in the “bridged cyclic group which maybe substituted with a group having a basic group, and also may have asubstituent(s)”.

In the present specification, the “C3-15 monocyclic or condensedcarbocyclic ring” in the “C3-15 monocyclic or condensed carbocyclic ringsubstituted with a group having a basic group” represented by ring J⁴has the same meaning as in the “C3-15 monocyclic or polycyclicunsaturated carbocyclic ring, and partially or completely saturated onethereof” in the “cyclic group”.

In the present specification, the “3- to 15-membered monocyclic orcondensed heterocyclic ring” of the “3- to 15-membered monocyclic orcondensed heterocyclic ring composed of carbon atom(s), oxygen atom(s)and/or sulfur atom(s) which may be oxidized, which is substituted with agroup having a basic group” represented by ring J⁴ includes a 3- to15-membered monocyclic or condensed unsaturated heterocyclic ringcomposed of carbon atom(s), oxygen atom(s) and/or sulfur atom(s) whichmay be oxidized, and partially or completely saturated one thereof.Examples thereof include, for example, furan, pyran, oxepine, thiophene,thiopyran, thiepine, benzofuran, isobenzofuran, benzothiophene,isobenzothiophene, dithianaphthalene, chromene, benzoxepine,benzothiepine, dibenzofuran, xanthene, dibenzothiophene, phenoxathiin,thianthrene, oxirane, oxetane, dihydrofuran, tetrahydrofuran,dihydropyran, tetrahydropyran, dihydrooxepine, tetrahydrooxepine,perhydrooxepine, thiirane, thietane, dihydrothiophene,tetrahydrothiophene, dihydrothiopyran, tetrahydrothiopyran,dihydrothiepine, tetrahydrothiepine, perhydrothiepine, oxathiane,dihydrobenzofuran, perhydrobenzofuran, dihydroisobenzofuran,perhydroisobenzofuran, dihydrobenzothiophene, perhydrobenzothiophene,dihydroisobenzothiophene, perhydroisobenzothiophene, benzoxathiane,benzodioxepane, dihydrodibenzofuran, dihydrodibenzothiophene,tetrahydrodibenzofuran, tetrahydrodibenzothiophene,perhydrodibenzofuran, perhydrodibenzothiophene, dioxolane, dioxane,dithiolane, dithiane, dioxaindane, benzodioxane, chroman,benzodithiolane, benzodithiane and the like.

In the present specification, the “3- to 15-membered monocyclic orcondensed heterocyclic ring which has at least one nitrogen atom andalso may have an oxygen atom(s) and/or a sulfur atom(s) which may beoxidized” of the “3- to 15-membered monocyclic or condensed heterocyclicring which has at least one nitrogen atom and also may have an oxygenatom(s) and/or a sulfur atom(s) which may be oxidized, which may besubstituted with a group having a basic group” represented by ring J⁴includes a monocyclic or condensed 3- to 15-membered unsaturatedheterocyclic ring which has at least one nitrogen atom and also may havean oxygen atom(s) and/or a sulfur atom(s) which may be oxidized, andpartially or completely saturated one thereof. Examples thereof include,for example, pyrrole, imidazole, triazole, tetrazole, pyrazole,pyridine, pyrazine, pyrimidine, pyridazine, azepin, diazepin, oxazole,isoxazole, thiazole, isothiazole, furazan, oxadiazole, oxazine,oxadiazine, oxazepine, oxadiazepine, thiadiazole, thiazine, thiadiazine,thiazepine, thiadiazepin, indole, isoindole, indolizine, indazole,quinoline, isoquinoline, quinolizine, purine, phthalazine, pteridine,naphthyridine, quinoxaline, quinazoline, cinnoline, benzoxazole,benzothiazole, benzimidazole, benzoxazepine, benzoxadiazepine,benzothiazepin, benzothiadiazepine, benzoxazepine, benzodiazepine,benzofurazan, benzothiadiazole, benzotriazole, carbazole, β-carboline,acridine, phenazine, phenothiazine, phenoxazine, phenanthridine,phenanthroline, perimidine, aziridine, azetidine, pyrroline,pyrrolidine, imidazoline, imidazolidine, triazoline, triazolidine,tetrazoline, tetrazolidine, pyrazoline, pyrazolidine, dihydropyridine,tetrahydropyridine, piperidine, dihydropyrazine, tetrahydropyrazine,piperazine, dihydropyrimidine, tetrahydropyrimidine, perhydropyrimidine,dihydropyridazine, tetrahydropyridazine, perhydropyridazine,dihydroazepine, tetrahydroazepine, perhydroazepine, dihydrodiazepine,tetrahydrodiazepine, perhydrodiazepine, dihydrooxazole,tetrahydrooxazole (oxazolidine), dihydroisoxazole, tetrahydroisoxazole(isoxazolidine), dihydrothiazole, tetrahydrothiazole (thiazolidine),dihydroisothiazole, tetrahydroisothiazole (isothiazolidine),dihydrofurazan, tetrahydrofurazan, dihydrooxadiazole,tetrahydrooxadiazole (oxadiazolidine), dihydrooxazine,tetrahydrooxazine, dihydrooxadiazine, tetrahydrooxadiazine,dihydrooxazepine, tetrahydrooxazepine, perhydrooxazepine,dihydrooxadiazepine, tetrahydrooxadiazepine, perhydrooxadiazepine,dihydrothiadiazole, tetrahydrothiadiazole (thiadiazolidine),dihydrothiazine, tetrahydrothiazine, dihydrothiadiazine,tetrahydrothiadiazine, dihydrothiazepine, tetrahydrothiazepine,perhydrothiazepine, dihydrothiadiazepine, tetrahydrothiadiazepine,perhydrothiadiazepine, morpholine, thiomorpholine, indoline,isoindoline, dihydroindazole, perhydroindazole, dihydroquinoline,tetrahydroquinoline, perhydroquinoline, dihydroisoquinoline,tetrahydroisoquinoline, perhydroisoquinoline, dihydrophthalazine,tetrahydrophthalazine, perhydrophthalazine, dihydronaphthyridine,tetrahydronaphthyridine, perhydronaphthyridine, dihydroquinoxaline,tetrahydroquinoxaline, perhydroquinoxaline, dihydroquinazoline,tetrahydroquinazoline, perhydroquinazoline, dihydrocinnoline,tetrahydrocinnoline, perhydrocinnoline, dihydrobenzoxazine,dihydrobenzothiazine, pyrazinomorpholine, dihydrobenzoxazole,perhydrobenzoxazole, dihydrobenzothiazole, perhydrobenzothiazole,dihydrobenzimidazole, perhydrobenzimidazole, dihydrobenzazepine,tetrahydrobenzazepine, dihydrobenzodiazepine, tetrahydrobenzodiazepine,dihydrobenzoxazepine, tetrahydrobenzoxazepine, dihydrocarbazole,tetrahydrocarbazole, perhydrocarbazole, dihydroacridine,tetrahydroacridine, perhydroacridine, hexahydro-1H-pyrrolidine,octahydrocyclopenta[c]pyrrole, octahydrocyclopenta[b]pyrrole,octahydropyrrolo[3,2-b]pyrrole, octahydropyrrolo[3,4-c]pyrrole,hexahydro-2H-furo[3,2-b]pyrrole, hexahydro-2H-thieno[3,2-b]pyrrole,decahydroquinoline, decahydro-2,6-naphthylidine,octahydro-2H-quinolidine, octahydro-1H-pyrido[1,2-c]pyrimidine,octahydro-2H-1,4-benzooxazine, decahydro-1,5-naphthylidine,octahydro-1H-pyrrolo[3,4-b]pyridine, octahydro-1H-pyrrolo[3,4-c]pyridineand the like.

The “group having a basic group” in ring J⁴ has the same meaning as inthe “group having a basic group” in the above-described “cyclic groupwhich is substituted with a group having a basic group, and also mayhave a substituent(s)”.

in the group, L^(A1) represents -(aliphatic hydrocarbon group having 1to 3 carbon atom(s) which may have a substituent(s))-(nitrogen atomwhich may have a substituent(s))-;

ring J^(1a) and ring J^(2a) each independently represents (i) a C3-10monocyclic or bicyclic carbocyclic ring, or (ii) a 3- to 10-memberedmonocyclic or bicyclic heterocyclic ring composed of a carbon atom(s),an oxygen atom(s) and/or a sulfur atom(s) which may be oxidized;

R represents a group having a basic group;

ring J^(1a) and ring J^(2a) may have the number of substituents capableof substituting on the substitutable position and,

when two or more substituents are present, plural substituents may bethe same or different,

wherein (a nitrogen atom which may have a substituent) in L^(A) isbonded to ring J¹),

in the group, L^(A2) represents a divalent aliphatic hydrocarbon grouphaving 1 to 4 carbon atom(s) which may have a substituent(s);

ring J^(1b) represents a 3- to 10-membered monocyclic or bicyclicheterocyclic ring which has at least one nitrogen atom and may also havean oxygen atom(s) and/or a sulfur atom(s) which may be oxidized;

ring J^(1b) may have the number of substituents capable of substitutingon the substitutable position and,

when two or more substituents are present, plural substituents may bethe same or different;

other symbols have the same meanings as described above),

in the group, ring J^(2b) represents a 3- to 10-membered monocyclic orbicyclic heterocyclic ring which may have at least one nitrogen atom andalso may have an oxygen atom(s) or a sulfur atom(s) which may beoxidized, which may be substituted with a group having a basic group;

ring J^(2b) may have the number of substituents capable of substitutingon the substitutable position and, when two or more substituents arepresent, plural substituents may be the same or different;

other symbols have the same meanings as described above), or

in the group, all symbols have the same meanings as described above.

includes, for example,

includes, for example,

wherein L^(A2) may be a substituent of a nitrogen atom of —NH—,

includes, for example,

wherein a nitrogen atom of —NH— may have a substituent, and

includes, for example,

wherein L^(A2) may be a substituent of a nitrogen atom of —NH—, and thenitrogen atom of —NH— may have a substituent.

In the present specification, the “group having a basic group” of R hasthe same meaning as in the “group having a basic group” of the“spiro-bound cyclic group which may be substituted with a group having abasic group, and also may have a substituent(s)”.

In the present specification, the “substituent” of the “number ofsubstituents capable of substituting on the substitutable position” ofring J¹, ring J², ring J³, ring J⁴, ring J^(1a), ring J^(1b), ringJ^(2a) and ring J^(2b) is not specifically limited. Examples thereofinclude those exemplified as for T in A¹ and A², and these optionalsubstituents may be substituted on the substitutable position and thenumber of substituents is preferably from 1 to 8, and more preferablyfrom 1 to 5.

In the present specification, the “substituent” in the “carbon atomwhich may have a substituent(s)” represented by D and G is notspecifically limited. Examples thereof include those exemplified as forT in A¹ and A², and these optional substituents may be substituted onthe substitutable position and the number of substituents is from 1 to2.

In the present specification, the “divalent nitrogen atom which may havea substituent” represented by G has the same meaning as in the “divalentnitrogen atom which may have a substituent”.

In the present specification, the “sulfur atom which may be oxidized”means —S—, —SO—, or —SO₂—.

In the present specification, the “substituent” represented by R¹ and R⁴is not specifically limited. Examples thereof include (1) aliphatichydrocarbon group which may have a substituent(s), (2) cyclic groupwhich may have a substituent(s), (3) aliphatic hydrocarbon groupsubstituted with a cyclic group which may have a substituent(s), and (4)substituent T described above. The “aliphatic hydrocarbon group” and“cyclic group” in the “aliphatic hydrocarbon group which may have asubstituent(s)”, “cyclic group which may have a substituent(s)” and“aliphatic hydrocarbon group substituted with a cyclic group which mayhave a substituent(s)” have the same meanings as described above, andexamples of the “substituent” include those exemplified as for T in A¹and A².

In the present specification, the “substituent” represented by R² and R³has the same meaning as in the “substituent” in (e) a mono- ordi-substituted amino group among the “basic group” of the “group havinga basic group” represented by A¹ and A².

The ring, which is formed by combining R² and R³ with the nitrogen atomto which they are attached, has the same meaning as in the “3- to10-membered monocyclic or condensed heterocyclic ring which has at leastone nitrogen atom and also may have an oxygen atom(s) and/or a sulfuratom(s) which may be oxidized” represented by ring J¹.

Examples of the “R² and R³ which may be combined with the nitrogen atomto which R² and R³ are attached to form a monocyclic or condensedheterocyclic ring, provided that an atom other than a nitrogen atom tobe attached, which constitutes a heterocyclic ring, is a carbon atom”include pyrrole, indole, isoindole, benzoxazepine, carbazole, acridine,aziridine, azetidine, pyrroline, pyrrolidine, dihydropyridine,tetrahydropyridine, piperidine, dihydroazepine, tetrahydroazepine,perhydroazepine, indoline, isoindoline, dihydroquinoline,tetrahydroquinoline, perhydroquinoline, dihydroisoquinoline,tetrahydroisoquinoline, perhydroisoquinoline, dihydrobenzoxazepine,tetrahydrobenzoxazepine, dihydrocarbazole, tetrahydrocarbazole,perhydrocarbazole, dihydroacridine, tetrahydroacridine and the like.

In the present invention, all isomers are included unless otherwisespecified. For example, alkyl group, alkenyl group, alkynyl group,alkylene group, alkenylene group, alkynylene group, alkylidene group andthe like include those which are linear and branched. Furthermore, allof isomers (E-, Z-, cis-, and trans-isomers) on the double bond, ringand condensed ring, isomers (R-isomer, S-isomer, α,β configuration,enantiomer, and diastereomer) due to the presence of asymmetric carbon,optically active substances with optical rotation (D-, L-, d-, andl-compounds), polar compounds (high polar compound and low polarcompound) generated by chromatographic separation, equilibriumcompounds, rotational isomers, mixtures in an optional mixing ratio andracemic mixtures are included in the present invention.

In the present invention, as is apparent to those skilled in the art,the symbol

represents that it is bonded to the other side of the page (namely, αconfiguration), the symbol

represents that it is bonded to this side of the page (namely, βconfiguration), and the symbol

represents that it is a mixture of the α configuration and the βconfiguration.

[Salts]

Salts of the compound represented by formula (I-0) include all ofnontoxic salts and pharmaceutically acceptable salts. Thepharmaceutically acceptable salt is preferably a water soluble saltwhich shows less toxicity. Examples of the suitable salt of the compoundrepresented by formula (I-0) include salts of alkali metal (potassium,sodium, lithium, etc.), salts of alkali earth metal (calcium, magnesium,etc.), ammonium salts (tetramethylammonium salt, tetrabutylammoniumsalt, etc.), salts of organic amine (triethylamine, methylamine,dimethylamine, cyclopentylamine, benzylamine, phenethylamine,piperidine, monoethanolamine, diethanolamine,tris(hydroxymethyl)methylamine, lysine, arginine, N-methyl-D-glucamine,etc.), acid addition salts [inorganic acid salts (hydrochloride,hydrobromide, hydroiodide, sulfate, phosphate, nitrate, etc.), andorganic acid salts (acetate, trifluoroacetate, lactate, tartrate,oxalate, fumarate, maleate, benzoate, citrate, methanesulfonate,ethanesulfonate, benzenesulfonate, toluenesulfonate, isethionate,glucuronate, gluconate, etc.)] and the like.

Furthermore, salts include quaternary ammonium salts. The quaternaryammonium salt is obtained by quaternizing a nitrogen atom of thecompound represented by formula (I-0) with a R⁰ group (R⁰ grouprepresents a C1-8 alkyl group, or a C1-8 alkyl group substituted with aphenyl group).

Also, salts include N-oxide. The compound of the present invention canbe converted into N-oxide by an optional method. N-oxide is obtained byoxidizing a nitrogen atom of the compound represented by formula (I-0).

Examples of suitable solvate of the compound represented by formula(I-0) include solvates such as water, alcoholic solvent (for example,methanol, ethanol, etc.) and the like. The solvate is preferablynontoxic and water soluble. The solvate of the compound of the presentinvention also includes solvates of alkali (earth) metal salts, ammoniumsalts, salts of organic amine, and acid addition salts of the compoundof the present invention.

The compound of the present invention can be converted into the abovesalts and solvates by a known method.

[Prodrugs]

A prodrug of the compound represented by formula (I-0) means a compoundwhich is converted into the compound represented by formula (I-0) in theliving body by the reaction with an enzyme, gastric acid or the like.Examples of the prodrug of the compound represented by formula (I-0)include compound wherein an amino group is acylated, alkylated, orphosphorylated (for example, compound wherein an amino group of thecompound represented by formula (I-0) is eicosanoylated, alanylated,pentylaminocarbonylated,(5-methyl-2-oxo-1,3-dioxolen-4-yl)methoxycarbonylated,tetrahydrofuranylated, pyrrolidylmethylated, pivaloyloxymethylated,acetoxymethylated, tert-butylated, etc.) when the compound representedby formula (I-0) has an amino group; compound wherein a hydroxyl groupis acylated, alkylated, phosphorylated, boricated or the like (forexample, compound wherein a hydroxyl group of the compound representedby formula (I-0) is acetylated, palmitoylated, propanoylated,pivaloylated, succinylated, fumarylated, alanylated,dimethylaminomethylcarbonylated, etc.) when the compound represented byformula (I-0) has a hydroxyl group; and compound wherein a carboxy groupis esterificated, amidated or the like (for example, compound wherein acarboxy group of the compound represented by formula (I-0) isethylesterificated, phenylesterificated, carboxymethylesterificated,dimethylaminomethylesterificated, pivaloyloxymethylesterificated,ethoxycarbonyloxyethylesterificated, phthalidylesterificated,(5-methyl-2-oxo-1,3-dioxolen-4-yl)methylesterificated,cyclohexyloxycarbonylethylesterificated, methylamidated, etc.) when thecompound represented by formula (I-0) has a carboxy group. Thesecompounds can be prepared by a per se known method. The prodrug of thecompound represented by formula (I-0) may be either of a hydrate and anon-hydrate. Also, the prodrug of the compound represented by formula(I-0) may be converted into the compound represented by formula (I-0)under physiological conditions described in “Development of Drug”published in 1990 by Hirokawa Shoten, Vol. 7, “Molecular Design”, pp.163-198. Furthermore, the compound represented by formula (I-0) may belabelled with isotope (for example, ³H, ¹⁴C, ³⁵S, ¹²⁵I, etc.) and thelike. The compound represented by formula (I-0) of the presentinvention, a salt thereof, a solvate thereof, or a prodrug thereof(hereinafter abbreviated to a compound of the present invention,sometimes) is a compound which is excellent in solubility and oralabsorption and maintain its pharmacological activity for a long periodof time, and is also less likely to be inhibited by a drug metabolizingenzyme and has low toxicity. These properties are most importantphysical, chemical and pharmacological properties required whenpreparations are developed, and the inventive compound satisfies theseconditions and is expected to be useful for developing extremelyexcellent (see The Merck Manual of Diagnosis and Therapy (17th Ed.),Merck & Co.).

The fact that the compound of the present invention is useful as a drugcan be evaluated by methods described in various tests and biologicalexamples described hereinafter, and methods which can be carried out byappropriately improving the above methods. The fact that the compound ofthe present invention is kinetically excellent in length of half-life inblood, stability in alimentary canal, oral absorption andbioavailability can be easily evaluated by a known method, for example,a method described in “Drug Bioavailability (Science of Evaluation andImprovement)”, Gendai Iryo-sha, published on Jul. 6, 1998.

In the formula (I-0) of the present invention, any of each definition byA¹, A², B¹, B², D, G, E, L, and J⁰ is preferred. In the following,preferable groups will be listed. The symbols used herein have the samemeaning as described above.

A¹ and A² each is preferably a basic group, and more preferably anitrogen-containing heterocyclic ring which may have a substituent(s),for example. The “nitrogen-containing heterocyclic ring group” herein ispreferably pyrrole, imidazole, triazole, pyrazole, pyridine, pyrazine,pyrimidine, benzimidazole, azabenzimidazole, or tetrahydroquinolinering, more preferably imidazole or benzimidazole ring, and particularlypreferably imidazol-2-yl or benzoimidazol-2-yl. The “substituent” hereinis preferably none or an aliphatic hydrocarbon group, more preferablynone, a C1-8 alkyl group or the like, and particularly preferably noneor a methyl group, and most preferably none. A¹ and A² may be the sameor different.

B¹ and B² each is preferably a spacer having a main chain of 1 atom, andmore preferably —CO—, SO₂— or a methylene group (—CH₂—) which may have asubstituent(s). The “substituent” herein is preferably none or a methylgroup, and more preferably none. B¹ and B² may be the same or different.

D is preferably a nitrogen atom or a carbon atom which may have asubstituent, and more preferably a nitrogen atom.

G is preferably a carbon atom which may have a substituent(s), acarbonyl group, or a sulfur atom which may be oxidized. The“substituent” herein is preferably none or a methyl group, and morepreferably none. G is more preferably a carbonyl group (—CO—), asulfonyl group (—SO₂—), or a methylene group which may have asubstituent(s). The “substituent” in the “methylene group which may havea substituent(s)” is not specifically limited and examples thereofinclude those exemplified as for the “substituent” in A¹ and A², andthese optional substituents may be substituted on the substitutableposition and the number of substituents is from 1 to 2. The “methylenegroup which may have a substituent(s)” does not represent a carbonylgroup (—CO—).

E is preferably a divalent aliphatic hydrocarbon group having 1 to 4carbon atom(s) which may have a substituent(s), a divalent 3- to8-membered monocyclic cyclic group which may have a substituent(s), or adivalent 9- or 10-membered polycyclic cyclic group may have asubstituent(s), and more preferably a divalent 3- to 8-memberedmonocyclic cyclic group which may have a substituent(s), or a divalent9- or 10-membered polycyclic cyclic group which may have asubstituent(s). The “divalent 3- to 8-membered monocyclic cyclic groupwhich may have a substituent(s)” has the same meaning as described inthe above described B¹. The “3- to 8-membered monocyclic cyclic group”herein is preferably a C5-7 monocyclic carbocyclic ring (ring having 5to 7 carbon atoms is selected from among the above described C3-8monocyclic carbocyclic ring) or a 5- to 7-membered monocyclicheterocyclic ring (5- to 7-membered heterocyclic ring is selected fromamong the above described 3- to 8-membered monocyclic heterocyclicring), more preferably, a cyclopentane, cyclohexane, cyclohexene,cyclohexadiene, benzene, pyridine, pyrazine, pyrimidine, pyridazine,piperidine or piperazine ring, and particularly preferably a benzenering.

Examples of the “divalent 9- or 10-membered polycyclic cyclic group” inthe “divalent 9- or 10-membered polycyclic cyclic group which may have asubstituent(s)” include divalent group obtained by eliminating optionaltwo hydrogen atoms from the “9- or 10-membered polycyclic cyclic group”.Examples of the “9- or 10-membered polycyclic cyclic group” hereininclude “9- or 10-membered polycyclic carbocyclic ring” and “9- or10-membered polycyclic heterocyclic ring”. The “9- or 10-memberedpolycyclic carbocyclic ring” includes a 9- or 10-membered polycyclicunsaturated carbocyclic ring, and partially or completely saturated onethereof. Examples of the “9- or 10-membered polycyclic unsaturatedcarbocyclic ring, and partially or completely saturated one thereof”include azulene, naphthalene, perhydroazulene, indene, perhydroindene,indane, dihydronaphthalene, tetrahydronaphthalene, andperhydronaphthalene rings.

Examples of the “9- or 10-membered polycyclic heterocyclic ring” include“9- or 10-membered polycyclic heterocyclic ring having, as a heteroatom, 1 to 4 nitrogen atom(s), 1 to 2 oxygen atom(s) and/or 1 to 2sulfur atom(s)”. The “9- or 10-membered polycyclic heterocyclic ringhaving, as a hetero atom, 1 to 4 nitrogen atom(s), 1 to 2 oxygen atom(s)and/or 1 to 2 sulfur atom(s)” includes a 9- or 10-membered polycyclicunsaturated heterocyclic ring having, as a hetero atom, 1 to 4 nitrogenatom(s), 1 to 2 oxygen atom(s) and/or 1 to 2 sulfur atom(s), andpartially or completely saturated one thereof. Examples of the “9- or10-membered polycyclic unsaturated heterocyclic ring having, as a heteroatom, 1 to 4 nitrogen atom(s), 1 to 2 oxygen atom(s) and/or 1 to 2sulfur atom(s), and partially or completely saturated one thereof”include indole, isoindole, indolizine, benzofuran, isobenzofuran,benzothiophene, isobenzothiophene, dithianaphthalene, indazole,quinoline, isoquinoline, quinolizine, purine, phthalazine, pteridine,naphthyridine, quinoxaline, quinazoline, cinnoline, benzoxazole,benzothiazole, benzimidazole, chromene, benzofurazan, benzothiadiazole,benzotriazole, indoline, isoindoline, dihydrobenzofuran,perhydrobenzofuran, dihydroisobenzofuran, perhydroisobenzofuran,dihydrobenzothiophene, perhydrobenzothiophene, dihydroisobenzothiophene,perhydroisobenzothiophene, dihydroindazole, perhydroindazole,dihydroquinoline, tetrahydroquinoline, perhydroquinoline,dihydroisoquinoline, tetrahydroisoquinoline, perhydroisoquinoline,dihydrophthalazine, tetrahydrophthalazine, perhydrophthalazine,dihydronaphthyridine, tetrahydronaphthyridine, perhydronaphthyridine,dihydroquinoxaline, tetrahydroquinoxaline, perhydroquinoxaline,dihydroquinazoline, tetrahydroquinazoline, perhydroquinazoline,dihydrocinnoline, tetrahydrocinnoline, perhydrocinnoline, benzoxathiane,dihydrobenzoxazine, dihydrobenzothiazine, pyrazinomorpholine,dihydrobenzoxazole, perhydrobenzoxazole, dihydrobenzothiazole,perhydrobenzothiazole, dihydrobenzimidazole, and perhydrobenzimidazolerings.

The “9- or 10-membered polycyclic cyclic group” herein is preferably a9- or 10-membered polycyclic heterocyclic ring group, and morepreferably a tetrahydroisoquinoline ring.

The “substituent” of the “divalent 3- to 8-membered monocyclic cyclicgroup which may have a substituent(s)” or the “divalent 9- or10-membered polycyclic cyclic group which may have a substituent(s)” ispreferably none, a halogen atom or a methyl group, and more preferablynone.

L is preferably a spacer having a main chain of 1 to 2 atom(s). The“spacer having a main chain of 1 to 2 atom(s)” is preferably a divalentgroup composed of 1 to 2 groups selected optionally from —O—, —S—, —CO—,—SO—, —SO₂—, divalent nitrogen atom which may have a substituent,divalent aliphatic hydrocarbon group having one carbon atom which mayhave a substituent(s), wherein 1 to 2 atom(s) of the main chain arearranged in a line, more preferably —CH₂—, —O—CH₂—, —S—CH₂—, —NH—CH₂—,—CH₂—CH₂—, —CH═CH—, —C≡C—, —CH₂—O—, —CH₂—S—, —CH₂—NH—, —CONH—, —SO₂—NH—,—NHCO— or —NHSO₂—, and particularly preferably —CH₂—, —CONH—, —CH₂—NH—,—O—CH₂—, —S—CH₂—, —CH₂—CH₂-(J^(o) is bonded to the right side). A bondis also preferable.

J⁰ is preferably an aliphatic hydrocarbon group which is substitutedwith a basic group, and also may have a substituent(s); a cyclic groupwhich is substituted with an aliphatic hydrocarbon group substitutedwith a basic group, and also may have a substituent(s); or an aliphatichydrocarbon group which is substituted with a cyclic group substitutedwith a basic group, and also may have a substituent(s). The “basicgroup” herein is preferably a mono- or di-substituted amino group, or anitrogen-containing heterocyclic ring which may have a substituent(s).The “mono- or di-substituted amino group” herein is preferably adi-substituted amino group, more preferably dimethylamino, diethylamino,dipropylamino, dibutylamino, or N-cyclohexyl-N-propylamino, andparticularly preferably dipropylamino or N-cyclohexyl-N-propylamino. The“nitrogen-containing heterocyclic ring which may have a substituent(s)”herein is preferably non-substituted nitrogen-containing heterocyclicring, or a nitrogen-containing heterocyclic ring substituted with a C1-8alkyl group or an oxo group, and the “nitrogen-containing heterocyclicring” is preferably a pyrrolidine, piperidine, morpholine,thiomorpholine, perhydrodiazepine, tetrahydroisoquinoline,2,8-diazaspiro[4.5]decane, 1,4,9-triazaspiro[5.5]undecane,3,9-diazaspiro[5.5]undecane, or 2,9-diazaspiro[5.5]undecane ring.

The “aliphatic hydrocarbon group” in the “aliphatic hydrocarbon groupwhich is substituted with a basic group, and also may have asubstituent(s)” or “aliphatic hydrocarbon group which is substitutedwith a cyclic group substituted with a basic group, and also may have asubstituent(s)” herein is preferably a C1-8 alkyl group or a C2-8alkenyl group, and more preferably methyl, ethyl, propyl, butyl, pentyl,or hexyl. The “cyclic group” in the “cyclic group which is substitutedwith an aliphatic hydrocarbon group substituted with a basic group, andalso may have a substituent(s)” is preferably a C5-7 monocycliccarbocyclic ring (ring having 5 to 7 carbon atoms is selected from amongthe above described C3-15 monocyclic or polycyclic carbocyclic ring) ora 5- to 7-membered monocyclic heterocyclic ring (5- to 7-memberedheterocyclic ring is selected from among the above described 3- to15-membered monocyclic or polycyclic heterocyclic ring), and morepreferably a cyclopentane, cyclohexane, cyclohexene, adamantyl,cyclohexadiene, benzene, pyridine, pyrazine, tetrahydropyran,pyrimidine, pyridazine, piperidine, or piperazine ring. The“substituent” herein is preferably none, a halogen atom, a methyl group,a hydroxyl group, an amino group or an oxo group, and more preferablynone.

Furthermore, J⁰ is preferably a “cyclic group which is substituted witha group having a basic group, and also may have a substituent(s)”, a“spirocyclic group which may be substituted with a group having a basicgroup, and also may have a substituent(s)”, or a “bridged cyclic groupwhich may be substituted with a group having a basic group, and also mayhave a substituent(s)”. The “cyclic group” or “spiro-bound cyclicgroup”, or “bridged cyclic group” is preferably (1) a spiro-bound cyclicgroup, (2) a bridged polycyclic carbocyclic ring, (3) a bridgedpolycyclic heterocyclic ring composed of a carbon atom(s), an oxygenatom(s) and/or a sulfur atom(s) which may be oxidized, (4) a bridgedpolycyclic heterocyclic ring which has at least one nitrogen atom, andalso may have an oxygen atom(s) and/or a sulfur atoms(s) which may beoxidized, (5) a C3-15 monocyclic or condensed carbocyclic ring, (6) a 3-to 15-membered monocyclic or condensed heterocyclic ring composed of acarbon atom(s), an oxygen atom(s) and/or a sulfur atom(s) which may beoxidized, or (7) a 3- to 15-membered monocyclic or condensedheterocyclic ring which has at least one nitrogen atom, and also has acarbon atom, oxygen atom and/or a sulfur atom which may be oxidized.

The “spiro-bound cyclic group” is preferably 2,7-diazaspiro[3.5]nonane,2,8-diazaspiro[4.5]decane, 2,7-diazaspiro[4.5]decane,2,9-diazaspiro[5.5]undecane, 2,8-diazaspiro[5.5]undecane,3,9-diazaspiro[5.5]undecane, 2,7-diazaspiro[4.4]nonane,1,2-dihydrospiro[indole-3,4′-piperidine],2,3-dihydro-1H-spiro[isoquinoline-4,4′-piperidine],1′,4′-dihydro-2′H-spiro[piperidine-4,3′-quinoline],2′,3′-dihydro-1′H-spiro[piperidine-4,4′-quinoline],8-azaspiro[4.5]decane, 8-azaspiro[4.5]decane, 7-azaspiro[4.5]decane,3-azaspiro[5.5]undecane, 2-azaspiro[5.5]undecane,1-oxa-4,8-diazaspiro[5.5]undecane, 1-oxa-4,9-diazaspiro[5.5]undecane,3,4-dihydrospiro[chromene-2,4′-piperidine], 2-azaspiro[4.4]nonane,7-azaspiro[3.5]nonane, 2,3-dihydrospiro[indene-1,4′-piperidine],3,4-dihydro-2H-spiro[naphthalene-1,4′-piperidine],3,4-dihydro-1H-spiro[naphthalene-2,4′-piperidine],2-azaspiro[4.5]decane, 2-azaspiro[3.5]nonane,1′,2′-dihydrospiro[cyclohexane-1,3′-indole],2′,3′-dihydro-1′H-spiro[cyclohexane-1,4′-isoquinoline],1′,4′-dihydro-2′H-spiro[cyclohexane-1,3′-quinoline],1,6-diazaspiro[3.4]octane, 1,5-diazaspiro[3.4]octane,1,7-diazaspiro[3.5]nonane, 1,6-diazaspiro[3.5]nonane,1,5-diazaspiro[3.5]nonane, 1,7-diazaspiro[4.4]nonane,1,6-diazaspiro[4.4]nonane, 1,8-diazaspiro[4.5]decane,1,7-diazaspiro[4.5]decane, 2,6-diazaspiro[3.4]octane,1,6-diazaspiro[4.5]decane, 2,6-diazaspiro[3.5]nonane,1,9-diazaspiro[5.5]undecane, 1,8-diazaspiro[5.5]undecane,6-azaspiro[3.5]nonane, 6-azaspiro[3.4]octane, 2-azaspiro[3.4]octane,1,7-diazaspiro[5.5]undecane, 1,4,9-triazaspiro[5.5]undecane,1,3,8-triazaspiro[4.5]decane, 1-thia-4,9-diazaspiro[5.5]undecane,1-thia-4,8-diazaspiro[5.5]undecane, spiro[4.4]nonane, spiro[4.5]decane,spiro[5.5]undecane, spiro[3.4]octane, or spiro[3.5]nonane. The“spiro-bound cyclic group” is more preferably 2,7-diazaspiro[3.5]nonane,2,8-diazaspiro[4.5]decane, 2,7-diazaspiro[4.5]decane,2,9-diazaspiro[5.5]undecane, 2,8-diazaspiro[5.5]undecane,3,9-diazaspiro[5.5]undecane, 2,7-diazaspiro[4.4]nonane,1,2-dihydrospiro[indole-3,4′-piperidine],2,3-dihydro-1H-spiro[isoquinoline-4,4′-piperidine],1′,4′-dihydro-2′H-spiro[piperidine-4,3′-quinoline],2′,3′-dihydro-1′H-spiro[piperidine-4,4′-quinoline],8-azaspiro[4.5]decane, 1-oxa-4,8-diazaspiro[5.5]undecane,1-oxa-4,9-diazaspiro[5.5]undecane,3,4-dihydrospiro[chromene-2,4′-piperidine], 1,6-diazaspiro[3.4]octane,1,5-diazaspiro[3.4]octane, 1,7-diazaspiro[3.5]nonane,1,6-diazaspiro[3.5]nonane, 1,5-diazaspiro[3.5]nonane,1,7-diazaspiro[4.4]nonane, 1,6-diazaspiro[4.4]nonane,1,8-diazaspiro[4.5]decane, 1,7-diazaspiro[4.5]decane,2,6-diazaspiro[3.4]octane, 1,6-diazaspiro[4.5]decane,2,6-diazaspiro[3.5]nonane, 1,9-diazaspiro[5.5]undecane,1,8-diazaspiro[5.5]undecane, 1,7-diazaspiro[5.5]undecane,1,4,9-triazaspiro[5.5]undecane, 1,3,8-triazaspiro[4.5]decane,1-thia-4,9-diazaspiro[5.5]undecane, or1-thia-4,8-diazaspiro[5.5]undecane. The “spiro-bound cyclic group” isparticularly preferably 2,7-diazaspiro[4.5]decane,2,8-diazaspiro[4.5]decane, 2,8-diazaspiro[5.5]undecane,2,9-diazaspiro[5.5]undecane, 2,7-diazaspiro[3.5]nonane, or1-oxa-4,9-diazaspiro[5.5]undecane.

The “bridged polycyclic carbocyclic ring” is preferablybicyclo[2.1.1]hexane, bicyclo[2.2.1]heptane, bicyclo[2.2.2]octane,bicyclo[3.2.1]octane, adamantane, bicyclo[3.3.1]nonane,bicyclo[3.2.1]octane, or bicyclo[3.3.2]decane.

The “bridged polycyclic heterocyclic ring which has at least onenitrogen atom, and also may have an oxygen atom(s) and/or a sulfuratom(s) which may be oxidized” is preferably1-azatricyclo[3.3.1.1^(3,7)]decane, 3-azabicyclo[3.3.1]nonane, or3,7-diazabicyclo[3.3.1]nonane.

The “7- to 15-membered bicyclic spiro-bound heterocyclic ring wherein aring comprising the spiro-bound heterocyclic ring is a monocyclecomposed of at least one nitrogen atom and carbon atoms, and/or amonocycle composed of at least one nitrogen atom, one oxygen atom andcarbon atoms” is preferably a 9- to 11-membered bicyclic spiro-boundheterocyclic ring wherein a ring comprising the spiro-bound heterocyclicring is a monocycle composed of at least one nitrogen atom and carbonatoms, and/or a monocycle composed of at least one nitrogen atom, oneoxygen atom and carbon atoms.

The “9 to 11-membered bicyclic spiro-bound heterocyclic ring wherein aring comprising the spiro-bound heterocyclic ring is a monocyclecomposed of at least one nitrogen atom and carbon atom, and/or amonocycle composed of at least one nitrogen atom, one oxygen atom andcarbon atoms” is preferably 2,7-diazaspiro[4.5]decane,2,8-diazaspiro[4.5]decane, 2,8-diazaspiro[5.5]undecane,2,9-diazaspiro[5.5]undecane, 2,7-diazaspiro[3.5]nonane, or1-oxa-4,9-diazaspiro[5.5]undecane.

The “C3-15 monocyclic or condensed carbocyclic ring” is preferablycyclopropane, cyclobutane, cyclopentane, cyclohexane, cycloheptane,cyclooctane, cyclopentene, cyclohexene, cycloheptene, cyclooctene,cyclopentadiene, cyclohexadiene, cycloheptadiene, cyclooctadiene,benzene, pentalene, perhydropentalene, azulene, perhydroazulene, indene,perhydroindene, indane, naphthalene, dihydronaphthalene,tetrahydronaphthalene, perhydronaphthalene, or1,2,3,5,6,7-hexahydro-s-indacene. More preferably, it is cyclopentane,cyclohexane, or cyclooctane. Most preferably, it is cyclohexane.

The “3- to 15-membered monocyclic or condensed heterocyclic ringcomposed of a carbon atom(s), an oxygen atom(s) and/or a sulfur atom(s)which may be oxidized” is preferably the partially or completelysaturated 3- to 15-membered monocyclic or condensed heterocyclic ringcomposed of a carbon atom(s), an oxygen atom(s) and/or a sulfur atom(s)which may be oxidized.

The “3- to 15-membered monocyclic or condensed heterocyclic ring whichhas at least one nitrogen atom, and also has a carbon atom(s), an oxygenatom(s) and/or a sulfur atom(s) which may be oxidized” is preferably thepartially or completely saturated 3- to 15-membered monocyclic orcondensed heterocyclic ring which has at least one nitrogen atom andalso has a carbon atom(s), an oxygen atom(s) and/or a sulfur atom(s)which may be oxidized.

The “substituent” in the phrase “which may be substituted with thesubstituent” of ring J¹, ring J², ring J³, and ring J⁴ is preferably analiphatic hydrocarbon group, a cyclic group, or an aliphatic hydrocarbongroup substituted with a cyclic group, and more preferably an aliphatichydrocarbon group having 1 to 8 carbon atom(s), or a C3-10 monocyclic orbicyclic carbocyclic ring.

The “group having a basic group” in the phrase “which is substitutedwith a group having a basic group”, or “which may be substituted with abasic group” of ring J², ring J³, and ring J⁴ is preferably a mono- ordi-substituted amino group, or a nitrogen-containing heterocyclic ringwhich may have a substituent(s). The “monomer or di-substituted aminogroup” herein is preferably a di-substituted amino group, morepreferably dimethylamino, diethylamino, dipropylamino, dibutylamino, orN-cyclohexyl-N-propylamino, and particularly preferably dipropylamino orN-cyclohexyl-N-propylamino.

R is preferably a mono- or di-substituted amino group or anitrogen-containing heterocyclic ring which may have a substituent(s).The “mono- or di-substituted amino group” herein is preferably adi-substituted amino group, more preferably dimethylamino, diethylamino,dipropylamino, dibutylamino, or N-cyclohexyl-N-propylamino, andparticularly preferably dipropylamino or N-cyclohexyl-N-propylamino.

In the present invention, a compound of the formula (I-0), which has acombination of preferable groups listed above, is preferable.

Among the compound represented by formula (I-0), a preferable compoundincludes, for example, a compound represented by formula (I-1):

wherein all symbols have the same meanings as described above, formula(I-2):

wherein all symbols have the same meanings as described above, formula(I-3):

wherein a ring A^(1A) and a ring A^(2A) each independently represents anitrogen-containing heterocyclic ring which may have a substituent(s);

B^(1A), B^(2A) and G^(A) each independently represents —CO—, —SO₂— or—CH₂—;

a ring E^(A) represents divalent 3- to 8-membered monocyclic cyclicgroup which may have a substituent(s), or a divalent 9- or 10-memberedpolycyclic cyclic group which may have a substituent(s); and

other symbols have the same meanings as described above, provided thatany of B^(1A) or G^(A) represents —CO— or —SO₂—,

Formula (I-5-1):

wherein all symbols have the same meanings as described above,

Formula (I-5-2):

wherein all symbols have the same meanings as described above,

Formula (I-5-3):

wherein all symbols have the same meanings as described above,

Formula (I-5-4):

wherein all symbols have the same meanings as described above,

Formula (I-6):

wherein all symbols have the same meanings as described above,

Formula (I-7):

wherein all symbols have the same meanings as described above,

Formula (I-8-1):

wherein all symbols have the same meanings as described above,

Formula (I-8-2):

wherein all symbols have the same meanings as described above,

Formula (I-8-3):

wherein all symbols have the same meanings as described above,

Formula (I-8-4):

wherein all symbols have the same meanings as described above,

Formula (I-8-5):

wherein all symbols have the same meanings as described above,

Formula (I-8-6):

wherein all symbols have the same meanings as described above,

Formula (I-8-7):

wherein all symbols have the same meanings as described above,

Formula (I-9-1):

wherein all symbols have the same meanings as described above,

Formula (I-9-2):

wherein all symbols have the same meanings as described above,

Formula (I-9-3):

wherein all symbols have the same meanings as described above,

Formula (I-9-4):

wherein all symbols have the same meanings as described above,

Formula (I-10):

wherein all symbols have the same meanings as described above, or

Formula (I-11)

wherein all symbols have the same meanings as described above, a saltthereof, an N-oxide thereof or a solvate thereof or a prodrug thereof.

Among the compound represented by formula (I-0), more preferablecompound is a compound represented by formula (I-4-1a):

wherein all symbols have the same meanings as described above,

Formula (I-4-1b):

wherein all symbols have the same meanings as described above,

Formula (I-4-2):

wherein all symbols have the same meanings as described above,

Formula (I-4-3a):

wherein all symbols have the same meanings as described above,

Formula (I-4-3b):

wherein all symbols have the same meanings as described above,

Formula (I-4-4):

wherein all symbols have the same meanings as described above,

Formula (I-4-5):

wherein all symbols have the same meanings as described above,

Formula (I-4-6):

wherein all symbols have the same meanings as described above,

Formula (I-8-1):

wherein all symbols have the same meanings as described above, or

Formula (I-8-2):

wherein all symbols have the same meanings as described above, a saltthereof, an N-oxide thereof or a solvate thereof or a prodrug thereof.

The substituent of ring J^(2b) is preferably R¹.

R¹ is preferably a hydrogen atom, an aliphatic hydrocarbon group, acyclic group, or an aliphatic hydrocarbon group substituted with acyclic group. The aliphatic hydrocarbon group represented by R¹ ispreferably a C1-8 alkyl group. The cyclic group represented by R¹ ispreferably a C3-15 monocyclic or polycyclic unsaturated carbocyclicring, or partially or completely saturated one thereof. It is morepreferably a C3-8 monocyclic saturated carbocyclic ring, andparticularly cyclopropane, cyclobutane, cyclopentane, cyclohexane, orcycloheptane. The “cyclic group” such as aliphatic hydrocarbon groupsubstituted with a cyclic group is preferably thiophene.

R² or R³ is preferably a hydrogen atom, an aliphatic hydrocarbon group,a cyclic group, or an aliphatic hydrocarbon group substituted with acyclic group. The aliphatic hydrocarbon group represented by R² or R³ ispreferably a C1-8 alkyl group. The “cyclic group” of the cyclic group oraliphatic hydrocarbon group substituted with a cyclic group representedby R² or R³ is preferably a C3-15 monocyclic or polycyclic unsaturatedcarbocyclic ring, or partially or completely saturated one thereof. The“cyclic group” of the cyclic group, or aliphatic hydrocarbon groupsubstituted with a cyclic group represented by R² or R³ is preferably aC3-8 monocyclic saturated carbocyclic ring, and particularly preferablycyclopropane, cyclobutane, cyclopentane, cyclohexane, or cycloheptane.

The “heterocyclic ring” of the heterocyclic ring which may have asubstituent(s), which is formed by combining R² and R³ with the nitrogenatom to which they are attached, is preferably a 3- to 15-memberedmonocyclic or condensed heterocyclic ring which has at least onenitrogen atom and also may have an oxygen atom(s) and/or a sulfuratom(s) which may be oxidized. More preferably, those composed of onenitrogen atom and carbon atoms are selected from among 3- to 15-memberedmonocyclic or condensed heterocyclic rings which have at least onenitrogen atom and also may have an oxygen atom(s) and/or a sulfuratom(s) which may be oxidized. More preferably, it is pyrrolidine,piperidine, perhydroazepine, tetrahydroisoquinoline,tetrahydroquinoline, or perhydroisoquinoline.

R⁴ is preferably a hydrogen atom, an aliphatic hydrocarbon group, or analiphatic hydrocarbon group substituted with a substituent(s). Thesubstituent of the aliphatic hydrocarbon group substituted with asubstituent(s) represented by R⁴ is preferably a hydroxyl group. Thealiphatic hydrocarbon group or “aliphatic hydrocarbon group” of thealiphatic hydrocarbon group substituted with a substituent(s) ispreferably a C3-8 aliphatic hydrocarbon group.

Specific examples of the compound of the present invention includecompound shown in the following (1) to (64), compounds described inExamples, salts thereof, N-oxides thereof or solvates thereof orprodrugs thereof:

(1)N-[4-({[4-(diisopropylamino)butyl]amino}methyl)benzyl]-N-(1H-imidazol-2-ylmethyl)-1H-imidazole-2-carboxyamide;(2)4-({[4-(diisopropylamino)butyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzenesulfonamide; (3)N-[4-({[4-(diisopropylamino)butyl]amino}methyl)benzyl]-N-(1H-imidazol-2-ylmethyl)-1H-imidazole-2-sulfonamide;(4)N¹-[4-(diisopropylamino)butyl]-N⁴,N⁴-bis(1H-imidazol-2-ylmethyl)terephthalamide;(5)N⁵-[4-(diisopropylamino)butyl]-N²,N²-bis(1H-imidazol-2-ylmethyl)pyridine-2,5-dicarboxyamide;(6)N²-[4-(diisopropylamino)butyl]-N⁵,N⁵-bis(1H-imidazol-2-ylmethyl)pyridine-2,5-dicarboxyamide;(7)N²-[4-(diisopropylamino)butyl]-N⁵,N⁵-bis(1H-imidazol-2-ylmethyl)pyrazine-2,5-dicarboxyamide;(8)N⁵-[4-(diisopropylamino)butyl]-N²,N²-bis(1H-imidazol-2-ylmethyl)pyrimidine-2,5-dicarboxyamide;(9)N²-[4-(diisopropylamino)butyl]-N⁵,N⁵-bis(1H-imidazol-2-ylmethyl)pyrimidine-2,5-dicarboxyamide;(10)N²-[4-(diisopropylamino)butyl]-N⁵,N⁵-bis(1H-imidazol-2-ylmethyl)-1H-pyrrole-2,5-dicarboxyamide;(11)N²-[4-(diisopropylamino)butyl]-N⁵,N⁵-bis(1H-imidazol-2-ylmethyl)-1H-imidazole-2,5-dicarboxyamide;(12)N⁵-[4-(diisopropylamino)butyl]-N²,N²-bis(1H-imidazol-2-ylmethyl)-1H-imidazole-2,5-dicarboxyamide;(13)N⁵-[4-(diisopropylamino)butyl]-N²,N²-bis(1H-imidazol-2-ylmethyl)-1,3-oxazole-2,5-dicarboxyamide;(14)N²-[4-(diisopropylamino)butyl]-N⁵,N⁵-bis(1H-imidazol-2-ylmethyl)-1,3-oxazole-2,5-dicarboxyamide;(15)N²-[4-(diisopropylamino)butyl]-N⁵,N⁵-bis(1H-imidazol-2-ylmethyl)-1,3-thiazole-2,5-dicarboxyamide;(16)N⁵-[4-(diisopropylamino)butyl]-N²,N²-bis(1H-imidazol-2-ylmethyl)-1,3-thiazole-2,5-dicarboxyamide;(17)N²-[4-(diisopropylamino)butyl]-N⁵,N⁵-bis(1H-imidazol-2-ylmethyl)thiophene-2,5-dicarboxyamide;(18)N²-[4-(diisopropylamino)butyl]-N⁵,N⁵-bis(1H-imidazol-2-ylmethyl)furan-2,5-dicarboxyamide;(19)N²-[4-(diisopropylamino)butyl]-N⁵,N⁵-bis(1H-imidazol-2-ylmethyl)-1,3,4-oxadiazole-2,5-dicarboxyamide;(20)N³-[4-(diisopropylamino)butyl]-N⁵,N⁵-bis(1H-imidazol-2-ylmethyl)-1,2,4-oxadiazole-3,5-dicarboxyamide;(21)N³-[4-(diisopropylamino)butyl]-N⁵,N⁵-bis(1H-imidazol-2-ylmethyl)-1,2,4-thiadiazole-3,5-dicarboxyamide;(22)N⁵-[4-(diisopropylamino)butyl]-N³,N³-bis(1H-imidazol-2-ylmethyl)-1,2,4-oxadiazole-3,5-dicarboxyamide;(23)N⁵-[4-(diisopropylamino)butyl]-N³,N³-bis(1H-imidazol-2-ylmethyl)-1,2,4-thiadiazole-3,5-dicarboxyamide;(24)N²-[4-(diisopropylamino)butyl]-N⁵,N⁵-bis(1H-imidazol-2-ylmethyl)-1,3,4-thiadiazole-2,5-dicarboxyamide;(25)4-({[4-(diisopropylamino)butyl]amino}sulfonyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(26)4-{[5-(diisopropylamino)pentyl]sulfanyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(27)4-{[5-(diisopropylamino)pentyl]oxy}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide; (28)4-{[5-(diisopropylamino)pentyl]amino}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(29)4-[(1E)-6-(diisopropylamino)hexa-1-enyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(30)4-{[5-(diisopropylamino)pentanoyl]amino}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(31)4-({[4-(diisopropylamino)butyl]sulfonyl}amino)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(32)4-({[4-(diisopropylamino)butyl]sulfanyl}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(33)4-{[4-(diisopropylamino)butoxy]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(34)4-{[5-(diisopropylamino)pentyl]sulfonyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(35)4-({[4-(diisopropylamino)butyl]sulfonyl}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(36)4-[6-(diisopropylamino)-2-oxohexyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide; (37)4-[6-(diisopropylamino)hexa-1-ynyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(38)N-[4-(diisopropylamino)butyl]-4-[3-(1H-imidazol-2-yl)-2-(1H-imidazol-2-ylmethyl)propanoyl]benzamide;(39)N-[4-(diisopropylamino)butyl]-4-[3-(1H-imidazol-2-yl)-2-(1H-imidazol-2-ylmethyl)propyl]benzamide;(40)N-[4-(diisopropylamino)butyl]-4-{[2-(1H-imidazol-2-yl)-1-(1H-imidazol-2-ylmethyl)ethyl]sulfonyl}benzamide;(41)N-[4-(diisopropylamino)butyl]-4-[2-(1H-imidazol-2-yl)-1-(1H-imidazol-2-ylmethyl)ethoxy]benzamide;(42)N-[4-(diisopropylamino)butyl]-4-{[2-(1H-imidazol-2-yl)-1-(1H-imidazol-2-ylmethyl)ethyl]sulfanyl}benzamide;(43)N-[4-(diisopropylamino)butyl]-4-{[2-(1H-imidazol-2-yl)-1-(1H-imidazol-2-ylmethyl)ethyl]amino}benzamide;(44)N-[4-(diisopropylamino)butyl]-4-[3-(1H-imidazol-2-yl)-2-(1H-imidazol-2-ylmethyl)propa-1-enyl]benzamide;(45)N¹-[4-(diisopropylamino)butyl]-N⁴,N⁴-bis(1H-imidazol-2-ylmethyl)cyclohexane-1,4-dicarboxyamide;(46)N¹-[4-(diisopropylamino)butyl]-N³,N³-bis(1H-imidazol-2-ylmethyl)isophthalamide;(47)N-[4-({[4-(diisopropylamino)butyl]amino}methyl)benzyl]-N-(1H-imidazol-2-ylmethyl)-1H-imidazole-2-carboxyamide;(48)N¹,N¹-bis(1H-benzoimidazol-2-ylmethyl)-N⁴-[4-(diisopropylamino)butyl]terephthalamide;(49)N¹-[4-(diisopropylamino)butyl]-N⁴-(1H-imidazol-2-ylmethyl)-N⁴-[(1-methyl-1H-imidazol-2-yl)methyl]terephthalamide;(50)N¹-[4-(diisopropylamino)butyl]-N⁴,N⁴-bis[(1-methyl-1H-imidazol-2-yl)methyl]terephthalamide;(51)N¹-[4-(diisopropylamino)butyl]-N⁴-(1H-imidazol-2-ylmethyl)-N⁴-(pyridine-2-ylmethyl)terephthalamide;(52)N¹-[4-(diisopropylamino)butyl]-N⁴,N⁴-bis(pyridine-2-ylmethyl)terephthalamide;(53)N¹-[4-(diisopropylamino)butyl]-N⁴-(1H-imidazol-2-ylmethyl)-N⁴-(5,6,7,8-tetrahydroquinoline-8-yl)terephthalamide;(54)N¹-(1H-benzoimidazol-2-ylmethyl)-N⁴-[4-(diisopropylamino)butyl]-N¹-(5,6,7,8-tetrahydroquinoline-8-yl)terephthalamide;(55)N¹-{3-[(diisopropylamino)methyl]phenyl}-N⁴,N⁴-bis(1H-imidazol-2-ylmethyl)terephthalamide;(56)N¹-[3-(diisopropylamino)benzyl]-N⁴,N⁴-bis(1H-imidazol-2-ylmethyl)terephthalamide;(57)N¹-{3-[(diisopropylamino)methyl]cyclohexyl}-N⁴,N⁴-bis(1H-imidazol-2-ylmethyl)terephthalamide;(58)N¹-{[3-(diisopropylamino)cyclohexyl]methyl}-N⁴,N⁴-bis(1H-imidazol-2-ylmethyl)terephthalamide;(59)N¹-[4-(dimethylamino)butyl]-N⁴,N⁴-bis(1H-imidazol-2-ylmethyl)terephthalamide;(60)N¹-[4-(diethylamino)butyl]-N⁴,N⁴-bis(1H-imidazol-2-ylmethyl)terephthalamide;(61)N¹,N¹-bis(1H-imidazol-2-ylmethyl)-N⁴-(4-pyrrolidine-1-ylbutyl)terephthalamide;(62)N¹,N¹-bis(1H-imidazol-2-ylmethyl)-N⁴-(4-piperidine-1-ylbutyl)terephthalamide;(63)4-{[[4-(diisopropylamino)butyl](methyl)amino]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(64)N-[4-({[4-(diisopropylamino)butyl]amino}carbonyl)benzyl]-N-(1H-imidazol-2-ylmethyl)pyridine-2-carboxyamide,salts thereof, N-oxides thereof or solvates thereof or prodrugs thereof.

Examples of more preferable compound include compounds described inExamples, salts thereof, N-oxides thereof or solvates thereof orprodrugs thereof.

Examples of particularly preferable compound include (1)4-[({trans-4-[cyclohexyl(propyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(2)4-[(2-cyclohexyl-2,8-diazaspiro[4.5]dec-8-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(3)4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(4)4-[(2-cyclohexyl-2,8-diazaspiro[4.5]dec-8-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(5)4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(6)4-[(7-cyclohexyl-2,7-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(7)4-[(7-cyclohexyl-2,7-diazaspiro[4.4]nona-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(8)4-[(9-cyclohexyl-3,9-diazaspiro[5.5]undec-3-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(9)4-{[8-(cyclohexylmethyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(10)4-{[8-(cyclopropylmethyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(11)4-{[8-(cyclopentylmethyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(12)N,N-bis(1H-imidazol-2-ylmethyl)-4-{[8-(2-thienylmethyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}benzamide;(13)N,N-bis(1H-imidazol-2-ylmethyl)-4-{[8-(3-thienylmethyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}benzamide;(14)4-{[8-(3-fluorobenzyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(15)4-{[8-(4-fluorobenzyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(16)N-(1H-benzoimidazol-2-ylmethyl)-4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N-(1H-imidazol-2-ylmethyl)benzamide;(17)N-(1H-benzoimidazol-2-ylmethyl)-N-{4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]benzyl}-1H-imidazole-2-carboxamide;(18)4-[(8-cyclopentyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(19)N,N-bis(1H-imidazol-2-ylmethyl)-4-{[8-(2-naphthylmethyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}benzamide;(20)4-[(8-cycloheptyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(21)4-{[8-(2,3-dihydro-1H-inden-2-yl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(22)4-[(8-cyclobutyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(23)N,N-bis(1H-imidazol-2-ylmethyl)-4-[(8-isobutyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]benzamide;(24)4-[(2-cyclohexyl-2,8-diazaspiro[4.5]dec-8-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)-2-methylbenzamide;(25)N,N-bis(1H-imidazol-2-ylmethyl)-4-{[8-(3-methyl-2-butene-1-yl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}benzamide;(26)4-{[8-(2-ethylbutyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(27)N,N-bis(1H-imidazol-2-ylmethyl)-4-({8-[(3-methyl-2-thienyl)methyl]-2,8-diazaspiro[4.5]dec-2-yl}methyl)benzamide;(28)4-{[8-(1-ethylpropyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(29)N,N-bis(1H-imidazol-2-ylmethyl)-4-({8-[(5-methyl-2-thienyl)methyl]-2,8-diazaspiro[4.5]dec-2-yl}methyl)benzamide;(30)4-({8-[(5-chloro-2-thienyl)methyl]-2,8-diazaspiro[4.5]dec-2-yl}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(31)4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)-2-methoxybenzamide;(32)1-{4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]phenyl}-N,N-bis(1H-imidazol-2-ylmethyl)methaneamine;(33)3-chloro-4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(34)4-[(8-cyclooctyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(35)1-{4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)carbonyl]phenyl}-N,N-bis(1H-imidazol-2-ylmethyl)methaneamine;(36)4-[(7-cycloheptyl-2,7-diazaspiro[3.5]nona-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(37)(2E)-3-{4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]phenyl}-N,N-bis(1H-imidazol-2-ylmethyl)acrylamide;(38)3-{4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]phenyl}-N,N-bis(1H-imidazol-2-ylmethyl)propaneamide;(39)4-{[8-(cyclobutylmethyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(40)4-[({trans-4-[benzyl(propyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(41)4-[({trans-4-[cyclohexyl(ethyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(42)4-({[4-(3,4-dihydro-2(1H)-isoquinolinyl)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(43)N,N-bis(1H-imidazol-2-ylmethyl)-4-({[4-(1-piperidinyl)cyclohexyl]amino}methyl)benzamide;(44)4-({[4-(1-azepanyl)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(45)4-({[trans-4-(cyclohexylamino)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(46)N-(1H-benzoimidazol-2-ylmethyl)-4-[({trans-4-[cyclohexyl(propyl)amino]cyclohexyl}amino)methyl]-N-(1H-imidazol-2-ylmethyl)benzamide;(47)4-[({trans-4-[(1-ethylpropyl)(propyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(48)4-{[{trans-4-[cyclohexyl(propyl)amino]cyclohexyl}(methyl)amino]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(49)4-{[{trans-4-[cyclohexyl(propyl)amino]cyclohexyl}(ethyl)amino]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(50)4-{[{trans-4-[cyclohexyl(propyl)amino]cyclohexyl}(propyl)amino]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(51)4-{[{trans-4-[cyclohexyl(propyl)amino]cyclohexyl}(2-hydroxyethyl)amino]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(52)4-[({trans-4-[(1-ethylpropyl)(2-hydroxyethyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(53)4-({[trans-4-(dipropylamino)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)-2-methoxybenzamide;(54)3-chloro-4-({[trans-4-(dipropylamino)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(55)4-({[4-(1-azocanyl)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(56)4-({[4-(1-azocanyl)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(57)4-({[(1-cycloheptyl-4-piperidinyl)methyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(58)4-({[4-(dipropylamino)butyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(59)4-({[3-(dibutylamino)propyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(60)4-({[5-(dipropylamino)pentyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(61)4-({4-[(dipropylamino)methyl]-1-piperidinyl}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(62)4-({[4-(dipropylamino)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(63)4-({[3-(dipropylamino)propyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(64)4-[(2-benzyl-2,9-diazaspiro[5.5]undec-9-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(65)4-[(2-cyclohexyl-2,9-diazaspiro[5.5]undec-9-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;(66)₂-[4-(dipropylamino)butyl]-N,N-bis(1H-imidazol-2-ylmethyl)-1,2,3,4-tetrahydro-6-isoquinolinecarboxamide;(67)N-[4-({[4-(dipropylamino)butyl]amino}methyl)benzyl]-N-(1H-imidazol-2-ylmethyl)-1H-imidazole-2-carboxamide;(68)4-({[4-(dipropylamino)butyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzenesulfonamide, salts thereof, N-oxides thereof or solvates thereof orprodrugs thereof.

Examples of the most preferable compound include4-({[4-(dipropylamino)butyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(compound 10),4-({[3-(dibutylamino)propyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(compound 11),4-({[5-(dipropylamino)pentyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(compound 11-2),4-({4-[(dipropylamino)methyl]-1-piperidinyl}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(compound 11-3),4-({[4-(dipropylamino)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(compound 11-4),4-({[3-(dipropylamino)propyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(compound 11-10),4-[(2-benzyl-2,9-diazaspiro[5.5]undec-9-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(compound 11-17),4-[(2-cyclohexyl-2,9-diazaspiro[5.5]undec-9-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(compound 11-18),2-[4-(dipropylamino)butyl]-N,N-bis(1H-imidazol-2-ylmethyl)-1,2,3,4-tetrahydro-6-isoquinolinecarboxamide(compound 15),N-[4-({[4-(dipropylamino)butyl]amino}methyl)benzyl]-N-(1H-imidazol-2-ylmethyl)-1H-imidazole-2-carboxamide(compound 19),4-({[4-(dipropylamino)butyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzenesulfonamide (compound 38),4-[({trans-4-[cyclohexyl(propyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(compound 43-4),4-[(2-cyclohexyl-2,8-diazaspiro[4.5]dec-8-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(compound 49-1),4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(compound 49-2),4-[(2-cyclohexyl-2,8-diazaspiro[4.5]dec-8-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(compound 50-92),4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(compound 50-95),N,N-bis(1H-imidazol-2-ylmethyl)-4-{[8-(2-thienylmethyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}benzamide(compound 50-29),N-(1H-benzoimidazol-2-ylmethyl)-4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N-(1H-imidazol-2-ylmethyl)benzamide(compound 50-38),4-[(8-cycloheptyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(compound 50-49),N,N-bis(1H-imidazol-2-ylmethyl)-4-({8-[(3-methyl-2-thienyl)methyl]-2,8-diazaspiro[4.5]dec-2-yl}methyl)benzamide(compound 50-78),4-{[8-(1-ethylpropyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(compound 50-81),4-[({trans-4-[benzyl(propyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(compound 50-1),4-({[4-(1-azepanyl)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(compound 50-19, compound 50-20),N-(1H-benzoimidazol-2-ylmethyl)-4-[({trans-4-[cyclohexyl(propyl)amino]cyclohexyl}amino)methyl]-N-(1H-imidazol-2-ylmethyl)benzamide(compound 50-39),4-[({trans-4-[(1-ethylpropyl)(propyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(compound 50-41),4-{[{trans-4-[cyclohexyl(propyl)amino]cyclohexyl}(methyl)amino]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(compound 50-67),4-{[{trans-4-[cyclohexyl(propyl)amino]cyclohexyl}(2-hydroxyethyl)amino]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(compound 50-103),4-[({trans-4-[(1-ethylpropyl)(2-hydroxyethyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(compound 50-105), salts thereof, N-oxides thereof or solvates thereofor prodrugs thereof.

[Method for Producing Compound of the Present Invention]

The compound of the present invention represented by formula (I-0) canbe prepared by appropriately improving a known method, for example,methods shown below, methods described in Examples, and a methoddescribed in Comprehensive Organic Transformations: A Guide toFunctional Group Preparations, Second edition (written by Richard C.Larock, John Wiley & Sons Inc, 1999) and using improved methods incombination. In the following production methods, starting compounds maybe used in the form of a salt. As the salt, those described as a salt ofthe above described formula (I-0) are used.

Among the compound of the present invention represented by formula(I-0), a compound wherein

represents a single bond, D represents a nitrogen atom, and G representsa carbonyl group, namely, a compound represented by formula (I-A):

wherein all symbols have the same meanings as described above, can beprepared by subjecting a compound represented by formula (2):

wherein all symbols have the same meanings as described above, and acompound represented by formula (3):

wherein W represents a hydroxyl group or a chlorine atom; and

other symbols have the same meanings as described above, to theamidation reaction and if necessary to the deprotection reaction of aprotective group and/or the cleavage reaction from a resin.

This amidation reaction is known and examples thereof include:

(1) a method using an acyl halide,

(2) a method using a mixed acid anhydride, and

(3) a method using a condensing agent.

These methods are described in detail below.

(1) The method using an acyl halide is carried out, for example, byreacting carboxylic acid with an acid halogenating agent (oxalylchloride, thionyl chloride, etc.) in an organic solvent (chloroform,dichloromethane, diethylether, tetrahydrofuran, etc.) or in the absenceof the solvent at −20° C. to reflux temperature. Then the obtained acylhalide derivative may be with amine in the presence of a base (pyridine,triethylamine, N,N-dimethylaniline, 4-dimethylaminopyridine,diisopropylethylamine, etc.) in an organic solvent (chloroform,dichloromethane, diethylether, tetrahydrofuran, etc.) at 0 to 40° C.Alternatively, the obtained acyl halide can be reacted with amine in anorganic solvent (dioxane, tetrahydrofuran, etc.) at 0 to 40° C. using anaqueous alkali solution (sodium bicarbonate water or sodium hydroxidesolution, etc.).

(2) The method using a mixed acid anhydride is carried out, for example,by reacting carboxylic acid with an acyl halide (pivaloyl chloride,tosyl chloride, mesyl chloride, etc.) or an acid derivative (ethylchloroformate, butyl chloroformate, etc.) in the presence of a base(pyridine, triethylamine, N,N-dimethylaniline, 4-dimethylaminopyridine,diisopropylethylamine, etc.) in an organic solvent (chloroform,dichloromethane, diethylether, tetrahydrofuran, etc.) or in the absenceof the solvent at 0 to 40° C., and reacting the resulting mixed acidanhydride with amine in an organic solvent (chloroform, dichloromethane,diethylether, tetrahydrofuran, etc.) at 0 to 40° C.

(3) The method using a condensing agent is carried out, for example, byreacting carboxylic acid with amine in an organic solvent (chloroform,dichloromethane, dimethyl formamide, diethylether, tetrahydrofuran,etc.) or in the absence of the solvent at 0 to 40° C. in the presence orabsence of a base (pyridine, triethylamine, diisopropylethylamine,N,N-dimethylaniline, N-methylmorpholine, 4-dimethylaminopyridine, etc.),using a condensing agent (1,3-dicyclohexylcarbodiimide (DCC),1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide (EDC),1,1′-carbonyldiimidazole (CDI),O-(7-azabenzotriazol-1-yl)N,N,N′,N′-tetramethyluroniumhexafluorophosphate (HATU), 2-chloro-1-methylpyridiniumiodine,1-propylphosphonic acid cyclic anhydride (1-propanephosphonic acidcyclic anhydride, PPA), etc.) and using, or not using,1-hydroxybenztriazole (HOBt) or 1-hydroxy-7-azabenzotriazole (HOAt).

The reactions described in (1), (2) and (3) are preferably carried outunder an inert gas (argon, nitrogen, etc.) atmosphere on anhydrouscondition.

The deprotection reaction of a protective group can be carried out by aknown method, for example, a method described in Protective Groups inOrganic Synthesis (written by T. W. Greene, John Wiley & Sons Inc,1999). The protective group is not specifically limited as long as it isa group which is described in the above documents or other group thatcan be deprotected easily and selectively.

If the compound has a moiety to bind to a resin in the molecule and theresin is a polystyrene resin, the compound of the present invention canbe cleaved from the resin by the following method. The reaction forcleavage from the resin is known and can be carried out, for example, byreacting in an organic solvent (dichloromethane, 1,2-dichloroethane,toluene, etc.) at 0 to 100° C. using an acid (acetic acid,trifluoroacetic acid, hydrochloric acid, etc.).

If necessary, the procedure of converting into the objective salt may becarried out by a known method after this reaction.

Among the compound of the present invention represented by formula(I-0), a compound wherein

represents a single bond, D represents a nitrogen atom, and G representsa —SO₂— group, namely, a compound represented by formula (I-B):

wherein all symbols have the same meanings as described above, can beprepared by subjecting a compound represented by formula (2) and acompound represented by formula (4):

wherein X represents a halogen atom; and

other symbols have the same meanings as described above, to thesulfonamidation reaction, and if necessary to the deprotection reactionof a protective group and/or the cleavage reaction from a resin.

The sulfonamidation reaction is known and can be carried out by thefollowing method. For example, a sulfonyl halide can be synthesized byreacting a sulfonic acid with an acyl halide (oxalyl chloride, thionylchloride, phosphorous pentachloride, phosphorus trichloride orphosphorus oxychloride, or a mixture thereof, etc.) in an organicsolvent (chloroform, dichloromethane, dichloroethane, diethylether,tetrahydrofuran, methyl t-butyl ether, etc.) or in the absence of thesolvent at −20° C. to reflux temperature in the presence or absence ofdimethyl formamide, or reacting a thiol with a chlorine gas in anaqueous acid solution (for example, hydrochloric acid, sulfuric acid,nitric acid, acetic acid, etc.) at 0° C. to reflux temperature. Thesulfonyl halide thus synthesized can be reacted with amine in thepresence of a base (diisopropylethylamine, pyridine, triethylamine,N,N-dimethylaniline, 4-dimethylaminopyridine, etc.) in an organicsolvent (chloroform, dichloromethane, dichloroethane, diethylether,tetrahydrofuran, etc.) at 0 to 40° C. The deprotection reaction of aprotective group or the cleavage reaction from the resin can be carriedout by the same method as described above.

Among the compound of the present invention represented by formula(I-0), a compound wherein L represents an amide bond, namely, a compoundrepresented by formula (I-C-1):

wherein R^(C1) represents a hydrogen atom or a substituent in the“divalent nitrogen atom which may have a substituent” defined in L; and

other symbols have the same meanings as described above,

or formula (I-C-2):

wherein all symbols have the same meanings as described above, can beprepared by subjecting a compound represented by formula (5):

wherein all symbols have the same meanings as described above, and acompound represented by formula (6):

wherein all symbols have the same meanings as described above, to theamidation reaction, and if necessary to the deprotection reaction of aprotective group and/or the cleavage reaction from a resin, orsubjecting a compound represented by formula (7):

wherein all symbols have the same meanings as described above, and acompound represented by formula (8):

wherein all symbols have the same meanings as described above, to theamidation reaction, and if necessary to the deprotection reaction of aprotective group and/or the cleavage reaction from a resin.

The amidation reaction, the deprotection reaction of a protective group,or the cleavage reaction from the resin can be carried out by the samemethod as described above.

Among the compound of the present invention represented by formula(I-0), a compound wherein L represents a sulfonamide bond, namely, acompound represented by formula (I-D-1):

wherein all symbols have the same meanings as described above, orformula (I-D-2):

wherein all symbols have the same meanings as described above, can beprepared by subjecting a compound represented by formula (5) and acompound represented by formula (9):

wherein all symbols have the same meanings as described above, to thesulfonamidation reaction, and if necessary to the deprotection reactionof a protective group and/or the cleavage reaction from the resin, orsubjecting a compound represented by formula (7) and a compoundrepresented by formula (10):

wherein all symbols have the same meanings as described above, to thesulfonamidation reaction, and if necessary to the deprotection reactionof a protective group and/or the cleavage reaction from the resin.

The sulfonamidation reaction, the deprotection reaction of a protectivegroup, or the cleavage reaction from the resin can be carried out by thesame method as described above.

Among the compound of the present invention represented by formula(I-0), a compound wherein L represents —CH(—R^(E1))—NH— or—NH—CH(—R^(E1))— (in the group, R^(E1) represents a hydrogen atom or asubstituent in the [divalent aliphatic hydrocarbon group having 1 to 4carbon atom(s) which may have a substituent(s)] defined in L) which mayhave a substituent(s), namely, a compound represented by formula(I-E-1):

wherein all symbols have the same meanings as described above, orformula (I-E-2):

wherein all symbols have the same meanings as described above, can beprepared by subjecting a compound represented by formula (11):

wherein all symbols have the same meanings as described above, and acompound represented by formula (12):

wherein all symbols have the same meanings as described above, to thereductive amination reaction, and if necessary to the deprotectionreaction of a protective group and/or the cleavage reaction from theresin, or subjecting a compound represented by formula (13):H₂N-J⁰  (13)

wherein all symbols have the same meanings as described above, and acompound represented by formula (14):

wherein all symbols have the same meanings as described above, to thereductive amination reaction, and if necessary to the deprotectionreaction of a protective group and/or the cleavage reaction from theresin.

This reductive amination reaction is known and is carried out, forexample, in an organic solvent (dichloroethane, dichloromethane,dimethyl formamide, acetic acid, a mixture thereof, etc.) at 0 to 40° C.in the presence of a reducing agent (sodium triacetoxyborohydride,sodium cyanoborohydride, sodium borohydride, etc.). The deprotectionreaction of a protective group or the cleavage reaction from the resincan be carried out by the same method as described above.

Among the compound of the present invention represented by formula(I-0), a compound wherein a basic group in a J⁰ group is amono-substituted amino group, namely, a compound represented by formula(I-F):

wherein J^(F) represents a divalent aliphatic hydrocarbon group whichmay have a substituent(s), a divalent cyclic group which may have asubstituent, an aliphatic hydrocarbon group substituted with a divalentcyclic group which may have a substituent(s), a cyclic group substitutedwith a divalent aliphatic hydrocarbon group which may have asubstituent(s), divalent spiro-bound cyclic group which may have asubstituent(s), or a divalent bridged cyclic group which may have asubstituent(s);

R^(F1) represents a substituent in the “mono-substituted amino group”defined in J⁰; and

other symbols have the same meanings as described above, can be preparedby subjecting the compound prepared by the above method, namely, acompound represented by formula (I-F-1):

wherein all symbols have the same meanings as described above, and acompound represented by formula (15):R^(F1)—CHO  (15)

wherein all symbols have the same meanings as described above, to thereductive amination reaction, and if necessary to the deprotectionreaction of a protective group and/or the cleavage reaction from aresin.

The reductive amination reaction, the deprotection reaction of aprotective group, or the cleavage reaction from the resin can be carriedout by the same method as described above.

Among the compound of the present invention represented by formula(I-0), a compound wherein a basic group in a J⁰ group is adi-substituted amino group, namely, a compound represented by formula(I-G):

wherein R^(F2) represents a substituent in the “di-substituted aminogroup” defined in J⁰; and

other symbols have the same meanings as described above, can be preparedby subjecting the compound prepared by the above method, namely, acompound represented by formula (I-F) and a compound represented byformula (16):R^(F2)—CHO  (16)

wherein all symbols have the same meanings as described above, to thereductive amination reaction, and if necessary to the deprotectionreaction of a protective group and/or the cleavage reaction from aresin.

The reductive amination reaction, the deprotection reaction of aprotective group, or the cleavage reaction from the resin can beprepared by the same method as described above.

A compound represented by formula (I-G) wherein R^(F1) and R^(F2)represent the same substituent can be prepared by subjecting a compoundrepresented by formula (I-F-1) and 2 or more equivalents of a compoundrepresented by formula (15) or formula (16) to the reductive aminationreaction, and if necessary to the deprotection reaction of a protectivegroup and/or the cleavage reaction from a resin.

The reductive amination reaction, the deprotection reaction of aprotective group, or the cleavage reaction from the resin can beprepared by the same method as described above.

Among the compound of the present invention represented by formula(I-0), a compound wherein A¹ and A² represent an imidazol-2-yl group,namely, a compound represented by formula (I-H):

wherein all symbols have the same meanings as described above, can beprepared by subjecting a compound represented by formula (17):

wherein all symbols have the same meanings as described above, to thecyclization reaction, using [2,2-bis(methyloxy)ethyl]amine or[2,2-bis(ethyloxy)ethyl]amine, and if necessary to the deprotectionreaction of a protective group and/or the cleavage reaction from aresin.

This cyclization reaction is known and is carried out, for example, byimproving a method described in Synthesis, 2001, (10), 1546-1550. Forexample, it is carried out by reacting a nitrile compound in an organicsolvent (methanol, ethanol, etc.) at 0 to 40° C. in the presence of abase (sodium methoxide, sodium ethoxide, etc.), and reacting thesolution at 40 to 150° C. in the presence of an acetal and a dehydratingagent (acetic acid, etc.). The deprotection reaction of a protectivegroup or the cleavage reaction from the resin can be carried out by thesame method as described above.

Among the compound of the present invention represented by formula(I-0), a compound wherein

represents a double bond, D represents a carbon atom, and G represents anitrogen atom, namely, a compound represented by formula (I-J):

wherein all symbols have the same meanings as described above, can beprepared by subjecting a compound represented by formula (18):

wherein all symbols have the same meanings as described above, and acompound represented by formula (19):H₂N-E-L-J⁰  (19)

wherein all symbols have the same meanings as described above, to theimination reaction, and if necessary to the deprotection reaction of aprotective group and/or the cleavage reaction from a resin.

The imination reaction is known and is carried out, for example, in anorganic solvent (methanol, ethanol, dichloromethane, chloroform,dichloroethane, benzene, toluene, etc.) at 20° C. to reflux temperaturein the presence or absence of a dehydrating agent (anhydrous magnesiumsulfate, Molecular Sieve (trade name), etc.) and an acid (hydrochloricacid, acetic acid, etc.).

Among the compound of the present invention represented by formula(I-0), a compound wherein D is a nitrogen atom and a divalent groupadjacent to D in B¹ is —CO—, namely, a compound represented by formula(I-K):

wherein B^(1K) represents a bond or a spacer having 1 to 3 atom(s) inits main chain; and

other symbols have the same meanings as described above, can be preparedby subjecting a compound represented by formula (20):A²-B²—NH-G-E-L-J⁰  (20)

wherein all symbols have the same meanings as described above, and acompound represented by formula (21):

wherein all symbols have the same meanings as described above, to theamidation reaction, and if necessary to the deprotection reaction of aprotective group and/or the cleavage reaction from a resin.

The amidation reaction, the deprotection reaction of a protective group,or the cleavage reaction from the resin can be carried out by the samemethod as described above.

Among the compound of the present invention represented by formula(I-0), a compound wherein D is a nitrogen atom and a divalent groupadjacent to D in B¹ is —SO₂—, namely, a compound represented by formula(I-L):

wherein B^(1L) represents a bond or a spacer having 1 to 3 atom(s) inits main chain; and

other symbols have the same meanings as described above, can be preparedby subjecting a compound represented by formula (20) and a compoundrepresented by formula (22):

wherein all symbols have the same meanings as described above, to thesulfonamidation reaction, and if necessary to the deprotection reactionof a protective group and/or the cleavage reaction from a resin.

The sulfonamidation reaction, the deprotection reaction of a protectivegroup, or the cleavage reaction from the resin can be carried out by thesame method as described above.

Among the compound represented by formula (I-0), a compound

-   -   wherein        represents a single bond;    -   D represents a nitrogen atom;    -   G represents one carbon atom which may have a substituent(s),        and a divalent group adjacent to D in B¹; and    -   B² is —CH₂—, namely, a compound represented by formula (I-M):

wherein B^(K2) represents a bond or a spacer having 1 to 3 atom(s) inits main chain;

R^(M1) represents a hydrogen atom or a substituent in the “carbon atomwhich may have a substituent(s)” defined in G; and

other symbols have the same meanings as described above, can be preparedby subjecting a compound represented by formula (23):

wherein all symbols have the same meanings as described above, and acompound represented by formula (24):

wherein all symbols have the same meanings as described above, to thereductive amination reaction, and if necessary to the deprotectionreaction of a protective group and/or the cleavage reaction from theresin.

The reductive amination reaction, the deprotection reaction of aprotective group, or the method for cleavage from the resin can becarried out by the same reaction as described above.

A compound represented by formula (I-M) can be prepared by subjecting acompound represented by formula (25):

wherein all symbols have the same meanings as described above, and acompound represented by formula (26):A¹-B^(1K)—CHO  (26)

wherein all symbols have the same meanings as described above, to thereductive amination reaction, subjecting the obtained compound and acompound represented by formula (27):A²-B^(2K)—CHO  (27)

wherein all symbols have the same meanings as described above, to thereductive amination reaction, and if necessary to the deprotectionreaction of a protective group and/or the cleavage reaction from theresin.

The reductive amination reaction, the deprotection reaction of aprotective group, or the cleavage reaction from the resin can be carriedout by the same method as described above.

A compound represented by formula (I-M) wherein a A¹-B^(1K)—CH₂ groupand a A²-B^(2K)—CH₂ group represent the same substituent can be preparedby subjecting a compound represented by formula (25) and 2 or moreequivalents of a compound represented by formula (26) or formula (27) tothe reductive amination reaction, and if necessary to the deprotectionreaction of a protective group and/or the cleavage reaction from theresin.

The reductive amination reaction, the deprotection reaction of aprotective group, or the cleavage reaction from the resin can be carriedout by the same method as described above.

The compounds represented by formulas (2) to (27) used as other startingmaterials or reagents can be easily prepared by using per se knownmethods or known methods, for example, methods described inComprehensive Organic Transformations: A Guide to Functional GroupPreparations, Second edition (written by Richard C. Larock, John Wiley &Sons Inc, 1999) in combination.

In the respective reactions in the present specification, as is apparentto those skilled in the art, the reaction with heating can be carriedout using a water bath, an oil bath, a sand bath, or microwave.

In the respective reactions in the present specification, a solid phasesupported reagent obtained by supporting on a polymer (for example,polystyrene, polyacrylamide, polypropylene, polyethylene glycol, etc.)may be used.

In the respective reactions in the present specification, the reactionproduct can be purified by conventional purification means, for example,distillation under normal pressure or reduced pressure, high performanceliquid chromatography using a silica gel or magnesium silicate, thinlayer chromatography, ion-exchange resin, scavenger resin orchromatography or washing, or recrystallization. The purification may becarried out for every reaction, or may be carried out after thecompletion of some reactions.

In the reaction using a polystyrene resin in the present specification,the reaction product can be purified by conventional purificationmethods, for example, washing plural times with a solvent (N,N-dimethylformamide, dichloromethane, methanol, tetrahydrofuran, toluene, aceticacid/toluene, etc.).

[Toxicity]

The compound of the present invention has very low toxicity and isconsidered to be safe enough for pharmaceutical use.

[Application to Pharmaceuticals]

The compound of the present invention has CXCR4 antagonistic activity inan animal including human, particularly human, and is thereforeeffective, for example, for a preventive and/or therapeutic agent forinflammatory and immune diseases, allergic diseases, infections,particularly HIV infection, and diseases associated with the infection,psychoneurotic diseases, cerebral diseases, cardiovascular diseases,metabolic diseases, and cancerous diseases. Also, the compound is usefulas an agent for regeneration therapy for the purpose of in vitro or invivo amplification of stem cells for gene therapy as well as peripheralblood stem cells mobilization and tissue repair. The compound isparticularly useful as an agent for transplantation medical treatmentused in organ transplantation including bone marrow transplantation,peripheral blood stem cell transplantation and tissue repair among inthe regeneration therapy. Furthermore, the compound is useful as anantiangiogenic agent which is effective for prevention and/or treatmentof diseases associated with neoangiogenesis, such as retinopathy(diabetic retinopathy, aged macular degeneration, glaucoma, etc.) andcancer proliferation.

Examples of the inflammatory and immune disease include rheumatoidarthritis, arthritis, retinopathy, gout, replacement organ rejection,graft-versus-host disease (GVHD), nephritis, psoriasis, rhinitis,conjunctivitis, multiple sclerosis, ulcerative colitis, Crohn's disease,shock associated with bacterial infection, pulmonary fibrosis, systemicinflammatory response syndrome (SIRS), acute lung injury, and diabetes.

Examples of the allergic disease include asthma, atopic dermatitis,rhinitis, and conjunctivitis

Examples of the disease associated with infection, particularly HIVinfection, include acquired immunodeficiency syndrome (AIDS),candidiasis, Pneumocystis carinii pneumonia, Cytomegalovirus retinitis,Kaposi's sarcoma, malignant lymphoma, AIDS encephalopathy, and bacterialsepsis.

Examples of the psychoneurotic disease and cerebral disease includedementia including Alzheimer's disease, Parkinson's disease, stroke,cerebral infarction, cerebral hemorrhage, epilepsia, schizophrenia, andperipheral nerve disorder.

Examples of the cardiovascular disease include arteriosclerosis,ischemia reperfusion, hypertension, myocardial infarction, stenocardia,and heart failure.

Examples of the metabolic diseases include diabetes, osteoporosis,enlarged prostate, and frequent micturition.

Examples of the cancerous disease include malignant tumor such as breastcancer or malignant lymphoma, cancer metastasis, and myelosuppression orthrombocytopenia after radiation therapy/chemotherapy.

The compound of the present invention may be administered as aconcomitant drug by using in combination with other drugs for thepurpose of:

1) complementation and/or enhancement of the preventive and/ortherapeutic effects of the compound,

2) improvement of pharmacokinetics and absorption of the compound andreduction of the dosage, and/or

3) reduction of side effects of the compound.

Also, the compound of the present invention may be administered as aconcomitant drug by using in combination with other drugs the purpose of(1) complementation and/or enhancement of preventive and/or therapeuticeffects, (2) improvement of pharmacokinetics and absorption of thecompound and reduction of the dosage, and/or (3) reduction of sideeffects.

The concomitant drug of the compound of the present invention and otherdrugs may be administered in the form of a compounding agent(s)comprising both these components, or may be in the form of separately.In case of separately administering a preparation, simultaneousadministration and administration with time-lag are included. In case ofadministration with time-lag, other drugs may be administered after thecompound of the present invention is administered, or the compound ofthe present invention may be administered after other drugs may beadministered. The administration method may be the same or different.

The disease, on which the preventive and/or therapeutic effects areexerted by the concomitant drug, is not specifically limited, and may beany disease which complements and/or enhances the preventive and/ortherapeutic effects of the compound of the present invention.

A mass ratio of the compound of the present invention drug to otherdrugs is not specifically limited.

A combination of any two or more kinds other drugs may be administered.

The other drugs, which complements and/or enhances the preventive and/ortherapeutic effects of the compound of the present invention, includesnot only those which have ever been found based on the above describedmechanism, but also those which may be found in future.

Examples of the preventive and/or therapeutic agents for HIV infectionand acquired immunodeficiency syndrome, which is used in combination ofthe compound of the present invention, include reverse transcriptaseinhibitors, protease inhibitors, chemokine (for example, CCR2, CCR3,CCR4, CCR5, CXCR4, etc.) antagonists, CD4 antagonists, antibody againstsurface antigen of HIV (for example, HIV-1, HIV-2, etc.) and vaccine ofHIV (for example, HIV-1, HIV-2, etc.).

Examples of the reverse transcriptase inhibitors include (1) nucleosidereverse transcriptase inhibitors such as zidovudine (trade name:Retrovir), didanosine (trade name: Videx), zalcitabine (trade name:Hivid), stavudine (trade name: Zerit), lamivudine (trade name: Epivir),abacavir (trade name: Ziagen), didanosine (trade name: videx), adefovir,dipivoxil, emtricitabine (trade name: coviracil), tenofovir (trade name:viread), Combivir, Trizivir, truvada, or epzicom, (2) non-nucleosidereverse transcriptase inhibitors such as nevirapine (trade name:viramune), delavirdine (trade name: Rescriptor), efavirenz (trade name:Sustiva, Stocrin), or capravirine (AG1549).

Examples of the protease inhibitors include indinavir (trade name:Kurikisiban), ritonavir (trade name: norvir), nelfinavir (trade name:Viracept), saquinavir (trade name: Invirase, Fortovase), amprenavir(trade name: agenerase), lopinavir (trade name: Kaletra), atazanavir(trade name: Reyataz), fosamprenavir (trade name: lexiva), tipranavirand the like.

Examples of the chemokine antagonists include endogenous ligands of achemokine receptor, or derivatives and nonpeptidic low molecularcompounds thereof, or an antibody against a chemokine receptor.

Examples of the endogenous ligands of the chemokine receptor includeMIP-1α, MIP-1β, RANTES, SDF-1α, SDF-1β, MCP-1, MCP-2, MCP-4, eotaxin,MDC and the like.

Examples of the derivative of the endogenous ligands include AOP-RANTES,Met-SDF-1α, Met-SDF-1β and the like.

Examples of the antibody of the chemokine receptor include Pro-140 andthe like.

Examples of the CCR2 antagonists include compounds described inWO99/07351, WO99/40913, WO00/46195, WO00/46196, WO00/46197, WO00/46198,WO00/46199, WO00/69432, WO00/69815, and Bioorg. Med. Chem. Lett., 10,1803 (2000), and the like.

Examples of the CCR3 antagonists include compounds described inDE19837386, WO99/55324, WO99/55330, WO00/04003, WO00/27800, WO00/27835,WO00/27843, WO00/29377, WO00/31032, WO00/31033, WO00/34278, WO00/35449,WO00/35451, WO00/35452, WO00/35453, WO00/35454, WO00/35876, WO00/35877,WO00/41685, WO00/51607, WO00/51608, WO00/51609, WO00/51610, WO00/53172,WO00/53600, WO00/58305, WO00/59497, WO00/59498, WO00/59502, WO00/59503,WO00/62814, WO00/73327, and WO01/09088, and the like.

Examples of the CCR4 antagonists include compounds described inWO02/030357 and WO02/030358, and the like.

Examples of the CCR5 antagonists include compounds described inWO99/17773, WO99/32100, WO00/06085, WO00/06146, WO00/10965, WO00/06153,WO00/21916, WO00/37455, EP1013276, WO00/38680, WO00/39125, WO00/40239,WO00/42045, WO00/53175, WO00/42852, WO00/66551, WO00/66558, WO00/66559,WO00/66141, WO00/68203, JP2000-309598, WO00/51607, WO00/51608,WO00/51609, WO00/51610, WO00/56729, WO00/59497, WO00/59498, WO00/59502,WO00/59503, WO00/76933, WO98/25605, WO99/04794, WO99/38514 and Bioorg.Med. Chem. Lett., 10, 1803 (2000), TAK-779, SCH-351125 (SCH-C),SCH-417690 (SCH-D), UK-427857, GW 873140A (ONO-4128), TAK-220, TAK-652,and the like.

Examples of the CXCR4 antagonists include AMD-3100, AMD-070, T-22,KRH-1120, KRH-1636, KRH-2731, CS-3955, and compounds described inWO00/66112, WO2004/024697 and WO2004/052862, and the like.

Examples of the fusion inhibitors include T-20 (pentafuside) T-1249, andthe like.

Examples of the HIV integrase inhibitors include Equisetin, Temacrazine,PL-2500, V-165, NSC-618929, L-870810, L-708906 analog, S-1360, 1838 andthe like.

Examples of the Short Interfering RNA include Short Interfering RNAdirected against HIV-1, HIV-1 constituent protein, genome HIV RNA, andthe like.

The conventional clinical dosage of typical reverse transcriptaseinhibitors and protease inhibitors is, for example, as described below,but is not limited thereto in the present invention.

Zidovudine: 100 mg capsule, three times per day in a dosage of 200 mg;300 mg tablet, twice per day in a dosage of 300 mg;

Didanosine: 25 to 200 mg tablet, twice per day in a dosage of 125 to 200mg;

Zalcitabine: 0.375 mg to 0.75 mg tablet, three times per day in a dosageof 0.75 mg;

Stavudine: 15 to 40 mg capsule, twice per day in a dosage of 30 to 40mg;

Lamivudine: 150 mg tablet, twice per day in a dosage of 150 mg;

Abacavir: 300 mg tablet, twice per day in a dosage of 300 mg;

Nevirapine: 200 mg tablet, once per day for 14 days in a dosage of 200mg, followed by twice per day;

Delavirdine: 100 mg tablet, three times per day in a dosage of 400 mg;

Efavirenz: 50 to 200 mg capsule, once per day in a dosage of 600 mg;

Indinavir: 200 to 400 mg capsule, three times per day in a dosage of 800mg;

Ritonavir: 100 mg capsule, twice per day in a dosage of 600 mg;

Nelfinavir: 250 mg tablet, three times per day in a dosage of 750 mg;

Saquinavir: 200 mg capsule, three times per day in a dosage of 1,200 mg;

Amprenavir: 50 to 150 mg tablet, twice per day in a dosage of 1,200 mg.

Examples of the other drugs for complementation and/or enhancement ofthe preventive and/or therapeutic effects of the compound of the presentinvention against asthma include antihistaminic agents, antiallergicagents (chemical mediator release inhibitors, histamine antagonists,thromboxane synthetase inhibitors, thromboxane antagonists, Th2 cytokineinhibitors), steroids, bronchodilator agents (xanthine derivatives,sympathomimetic agents, parasympathomimetic agents), vaccinotherapeuticagents, gold preparations, Chinese medicines, basic nonsteroidalanti-inflammatory drugs, 5-lipoxygenase inhibitors, 5-lipoxygenaseactivation protein antagonists, leukotriene synthesis inhibitors,prostaglandins, cannabinoid-2 receptor stimulants, antitussive drugs,expectorants, and the like.

Examples of the antihistaminic agents include diphenhydramine,diphenylpyraline hydrochloride, diphenylpyraline chlorotheophyllinate,clemastine fumarate, dimenhydrinate, dl-chlorpheniramine maleate,d-chlorpheniramine maleate, triprolidine hydrochloride, promethazinehydrochloride, alimemazine tartrate, isothipendyl hydrochloride,homochlorcyclizine hydrochloride, hydroxyzine, cyproheptadinehydrochloride, levocabastine hydrochloride, astemizole, bepotastine,desloratadine, TAK-427, ZCR-2060, NIP-530, mometasone furoate,mizolastine, BP-294, andolast, auranofin, acrivastine and the like.

Examples of the chemical mediator release inhibitors include disodiumcromoglycate, tranilast, amlexanox, repirinast, ibudilast, pemirolastpotassium, tazanolast, nedocromil, cromoglicate, israpafant and thelike.

Examples of the histamine antagonists include ketotifen fumarate,azelastine hydrochloride, oxatomide, mequitazine, terfenadine,emedastine fumarate, epinastine hydrochloride, ebastine, cetirizinehydrochloride, olopatadine hydrochloride, loratadine, fexofenadine andthe like.

Examples of the thromboxane synthetase inhibitors include ozagrelhydrochloride imitrodast sodium and the like.

Examples of the thromboxane antagonists include seratrodast, ramatroban,domitroban calcium hydrate, KT-2-962 and the like.

Examples of the Th2 cytokine inhibitors include suplatast tosilate andthe like.

Examples of the steroids include, for example, external medicine such asclobetasol propionate, diflorasone diacetate, fluocinonide, mometasonefuroate, betamethasone dipropionate, betamethasone butyrate propionate,betamethasone valerate, difluprednate, budesonide, diflucortolonevalerate, amcinonide, halcinonide, dexamethasone, dexamethasonepropionate, dexamethasone valerate, dexamethasone acetate,hydrocortisone acetate, hydrocortisone butyrate, hydrocortisone butyratepropionate, deprodone propionate, prednisolone valerate acetate,fluocinolone acetonide, beclometasone dipropionate, triamcinoloneacetonide, flumetasone pivalate, alclometasone propionate, clobetasonebutyrate, prednisolone, beclomethasone propionate, fludroxycortide, andthe like.

Examples of drugs for internal use and injections include cortisoneacetate, hydrocortisone, hydrocortisone sodium phosphate, hydrocortisonesodium succinate, fludrocortisone acetate, prednisolone, prednisoloneacetate, prednisolone sodium succinate, prednisolone butylacetate,prednisolone sodium phosphate, halopredone acetate, methyl prednisolone,methylprednisolone acetate, methylprednisolone sodium succinate,triamcinolone, triamcinolone acetate, triamcinolone acetonide,dexamethasone, dexamethasone acetate, dexamethasone sodium phosphate,dexamethasone palmitate, paramethasone acetate, betamethasone and thelike.

Examples of the inhalations include beclometasone dipropionate,fluticasone propionate, budesonide, flunisolide, triamcinolone, ST-126P,ciclesonide, dexamethasone palmitate, mometasone furoate, prasteronesulfonate, deflazacort, methylprednisolone suleptanate,methylprednisolone sodium succinate and the like.

Examples of the xanthine derivative include aminophylline, theophylline,doxophylline, cipamfylline, diprophylline, proxyphylline, and cholinetheophylline.

Examples of the sympathomimetic agents include epinephrine, ephedrinehydrochloride, dl-methylephedrine hydrochloride, methoxyphenaminehydrochloride, isoproterenol sulfate, isoproterenol hydrochloride,orciprenaline sulfate, chloroprenaline hydrochloride, trimetoquinolhydrochloride, salbutamol sulfate, terbutaline sulfate, hexoprenalinesulfate, tulobuterol hydrochloride, procaterol hydrochloride, fenoterolhydrobromate, formoterol fumarate, clenbuterol hydrochloride, mabuterolhydrochloride, salmeterol xinafoate, R,R-formoterol, tulobuterol,pirbuterol hydrochloride, ritodrine hydrochloride, bambuterol,dopexamine hydrochloride, meluadrine tartrate, AR-C68397,levosalbutamol, KUR-1246, KUL-7211, AR-C89855, S-1319 and the like.

Examples of the parasympathomimetic agents include ipratropium bromide,flutropium bromide, oxitropium bromide, cimetropium bromide, temiverine,tiotropium bromide, revatropate (UK-112166) and the like.

Examples of the vaccinotherapeutic agents include paspat, asthremedin,Broncasma Berna, CS-560 and the like.

Examples of the gold preparations include gold sodium thiomalate and thelike.

Examples of the basic nonsteroidal anti-inflammatory drugs includetiaramide hydrochloride, tinoridine hydrochloride, epirizole, emorfazoneand the like.

Examples of the 5-lipoxygenase inhibitors include zyleuton, docebenone,piriprost, SCH-40120, WY-50295, E-6700, ML-3000, TMK-688, ZD-2138,dalbufelone mesilate, R-68151, E-6080, DuP-654, SC-45662, CV-6504,NE-11740, CMI-977, NC-2000, E-3040, PD-136095, CMI-392, TZI-41078,Orf-20485, IDB-18024, BF-389, A-78773, TA-270, FLM-5011, CGS-23885,A-79175, ETH-615 and the like.

Examples of the 5-lipoxygenase activation protein antagonists includeMK-591, MK-886 and the like.

Examples of the leukotriene synthesis inhibitors include auranofin,proglumetacin maleate, L-674636, A-81834, UPA-780, A-93178, MK-886,REV-5901A, SCH-40120, MK-591, Bay-x-1005, Bay-y-1015, DTI-0026,Amlexanox, E-6700 and the like.

Examples of the prostaglandins (hereinafter abbreviated to as PG)include PG receptor agonists, PG receptor antagonists and the like.

Examples of the PG receptor include PGE receptor (EP1, EP2, EP3, andEP4), PGD receptor (DP, CRTH2), PGF receptor (FP), PGI receptor (IP), TXreceptor (TP) and the like.

Examples of the antitussive drugs include codeine phosphate,dihydrocodeine phosphate, oxymetebanol, dextromethorphan hydrobromate,pentoxyverine citrate, dimemorfan phosphate, oxeladin citrate,cloperastine, benproperine phosphate, clofedanol hydrochloride,fominoben hydrochloride, noscapine, tipepidine hibenzate, eprazinonehydrochloride, plantago herb extract and the like.

Examples of the expectorants include foeniculated ammonia spirit, sodiumhydrogencarbonate, potassium iodide, bromhexine hydrochloride, cherrybark extract, carbocysteine, fudosteine, ambroxol hydrochloride,ambroxol hydrochloride sustained-release tablet, methylcysteinehydrochloride, acetylcysteine, L-ethylcysteine hydrochloride, tyloxapoland the like.

Examples of the other drugs for complementation and/or enhancement ofthe preventive and/or therapeutic effects against atopic dermatitis(urticaria, etc.) of the compound of the present invention includesteroids, non-steroid anti-inflammatory drug (NSAID), immune inhibitor,prostaglandins, antiallergic agent, mediator release inhibitor,antihistaminic agent, forskolin preparation, phosphodiesteraseinhibitor, and cannabinoid-2 receptor stimulant.

Examples of the other drugs for complementation and/or enhancement ofthe preventive and/or therapeutic effects against allergic diseases(allergic bronchopulmonary aspergillosis, allergic eoisinophilicgastroenteritis, etc.) of the compound of the present invention includeantiasthmatic drug, inhaled steroid drug, inhaled β2 stimulant,methylxanthine-based stimulant, antiallergic agent, anti-inflammatoryagent, anticholinergic agent, thromboxane antagonist, leukotrieneantagonist, LTD4 antagonist, PAF antagonist, phosphodiesteraseinhibitor, β2 agonist, steroid drug, mediator release inhibitor,eosinophile leukocytechemotaxis inhibitor, macrolide-based antibiotic,immune inhibitor, hyposensitization (allergen) injection and the like.

Examples of the antiasthmatic drug include theophylline, procaterol,ketotifen, azelastine and the like.

Examples of the inhaled steroid drug include beclomethasone,fluticasone, budesonide and the like.

Examples of the inhaled β2 stimulant include fenoterol, salbutamol,formoterol, salmeterol and the like.

Examples of the methylxanthine-based stimulant include theophylline andthe like.

Examples of the antiallergic agent include ketotifen, terfenadine,azelastine, epinastine, suplatast, disodium cromoglycate and the like.

Examples of the anti-inflammatory agent include dichlofenac sodium,ibuprofen, indomethacin and the like.

Examples of the anticholinergic agent include ipratropium bromide,flutropium bromide, oxitropium bromide, tiotropium bromide and the like.

Examples of the thromboxane antagonist include ozagrel, seratrodast andthe like.

Examples of the leukotriene antagonist include pranlukast, montelukast,z afirlukast, zyleuton and the like.

Examples of the macrolide-based antibiotic include erythromycin,roxithromycin and the like.

Examples of the immune inhibitor include cyclosporine, tacrolimus,FTY720, and the like.

Examples of the other drug for complementation and/or enhancement of thepreventive and/or therapeutic effects against hepatitis of the compoundof the present invention include liver hydro lysate preparation,polyenephosphatidylcholine, glycyrrhizin preparation, protoporphyrinsodium, ursodeoxycholic acid, steroids, anticholinergic agent, gastricantiacid, propagermanium, lipid peroxidase inhibitor, and mitochondrialbenzodiazepine receptor antagonist.

Examples of the other drug for complementation and/or enhancement of thepreventive and/or therapeutic effects against arthritis and rheumatoidarthritis of the compound of the present invention includemetalloproteinase inhibitor, immune inhibitor, non-steroidanti-inflammatory drug (NSAID), steroid drug, prostaglandins,phosphodiesterase inhibitor, cannabinoid-2 receptor stimulant, diseasemodifying anti-rheumatic drug (slow-acting anti-rheumatic drug),anti-inflammatory enzyme preparation, cartilage protective agent, T cellinhibitor, TNFα inhibitor, prostaglandin synthetase inhibitor, IL-6inhibitor, interferon γ agonist, IL-1 inhibitor and the like.

Examples of the other drug for complementation and/or enhancement of thepreventive and/or therapeutic effects against psoriasis of the compoundof the present invention include steroid drug, vitamin D derivative andthe like.

Examples of the other drug for complementation and/or enhancement of thepreventive and/or therapeutic effects against rhinitis of the compoundof the present invention include antihistaminic agent, mediator releaseinhibitor, thromboxane synthetase inhibitor, thromboxane A₂ receptorantagonist, leukotriene receptor antagonist, steroids, a adrenalinreceptor stimulant, xanthine derivative, anticholinergic agent,prostaglandins, nitrogen monoxide synthetase inhibitor, β₂ adrenalinreceptor stimulant, phosphodiesterase inhibitor, cannabinoid-2 receptorstimulant and the like.

Examples of the other drug for complementation and/or enhancement of thepreventive and/or therapeutic effects against conjunctivitis of thecompound of the present invention include leukotriene receptorantagonist, antihistaminic agent, mediator release inhibitor,non-steroid anti-inflammatory drug, prostaglandins, steroid drug,nitrogen monoxide synthetase inhibitor, cannabinoid-2 receptor stimulantand the like.

Examples of the other drug for complementation and/or enhancement of thepreventive and/or therapeutic effects against multiple sclerosis of thecompound of the present invention include immune inhibitor,cannabinoid-2 receptor stimulant and the like.

Examples of the other drug for complementation and/or enhancement of thepreventive and/or therapeutic effects against ulcerative colitis of thecompound of the present invention include mesalazine,salazosulfapyridine, digestive tract ulcer therapeutic substance,anticholinergic agent, steroid drug, 5-lipoxygenase inhibitor,antioxidant, LTB4 antagonist, local anesthetic, immune inhibitor,protection factor enhancer, MMP inhibitor, and mitochondrialbenzodiazepine receptor antagonist.

Examples of the other drug for complementation and/or enhancement of thepreventive and/or therapeutic effects against diabetic complication ofthe compound of the present invention include sulfonyl urea-basedhypoglycemic agent, biguanide-based drug, α-glucosidase inhibitor,ultrashort-acting insulinotropic agent, insulin drug, PPAR agonist,insulin sensitive enhancer having no PPAR antagonism, β3 adrenalinreceptor agonist, aldose reductase inhibitor, dipeptidyl peptidase IVinhibitor and the like.

Examples of the sulfonyl urea-based hypoglycemic agent includeacetohexamide, glibenclamide, gliclazide, glyclopyramide,chlorpropamide, tolazamide, tolbutamide, Glimepiride and the like.

Examples of the biguanide-based drug include buformin hydrochloride,metformin hydrochloride and the like.

Examples of the α-glucosidase inhibitor include acarbose, voglibose andthe like.

Examples of the ultrashort-acting insulinotropic agent includenateglinide, repaglinide and the like.

Examples of the PPAR agonist include pioglitazone, troglitazone,rosiglitazone, JTT-501, and the like.

Examples of the insulin sensitive enhancer having no PPAR antagonisminclude ONO-5816, YM-440 and the like.

Examples of the β3 adrenalin receptor agonist include AJ9677, L750355,and CP331648.

Examples of the aldose reductase inhibitor include epalrestat,fidarestat, zenarestat and the like.

Examples of the other drug for complementation and/or enhancement of thepreventive and/or therapeutic effects against cancer (malignant tumor)and cancer metastasis of the compound of the present invention includeanticancer agent (for example, MMP inhibitor, alkylation agent (forexample, cyclophosphamide, melphalan, thiotepa, mytomycin C, busulfan,procarbazine hydrochloride, etc.), antimetabolite (for example,methotrexate, mercaptpurine, azathiopurine, fluorouracil, tegafur,cytarabine, azaserine, etc.), antibiotic (for example, mytomycin C,bleomycin, Peplomycin, doxorubicin hydrochloride, aclarubicin,daunorubicin, actinomycin D, etc.), mitosis inhibitor, platinum complex(for example, Cisplatin), plant-derived antineoplastic agent (forexample, vincristine sulfate, vinblastine sulfate, etc.), anticanceroushormone (for example, methyltestosterone, testosterone propionate,testosterone enanthate, mepitiostane, fosfestrol, chlormadinone acetate,etc.), immunopotentiator (for example, picibanil, krestin, etc.), andinterferon (for example, IFNα, IFNα-2a, IFNα-2b, IFNβ, IFNγ-1a, etc.).Examples thereof include biologics capable of conducting T cellactivation (for example, anti-CTLA-4 antibody, anti-PD-1 antibody,etc.), antiangiogenic agent (for example, bevacizumab, pegaptanib,SU-6668, vatalanib, ranibizumab, sorafenib, SU-11248, neovastat), etc.),and the like.

Examples of the other drug for complementation and/or enhancement oftreatment including bone marrow transplantation, peripheral blood stemcell transplantation and tissue repair of the compound of the presentinvention include G-CSF, AMD3100 and the like.

Examples of the other drug for complementation and/or enhancement of thepreventive and/or therapeutic effects against immune disease (forexample, autoimmune disease, transplanted organ rejection, etc.) of thecompound of the present invention include immune inhibitor (for example,cyclosporine, tacrolimus, FTY720, etc.).

Examples of the other drug for complementation and/or enhancement of thepreventive and/or therapeutic effects against dementia such as Seniledementia with Alzheimer's type of the compound of the present inventioninclude acetylcholine esterase inhibitor, nicotinic receptor modifier,cerebral ameliorator, monoamineoxidase inhibitor, vitamin E, aldosereductase inhibitor and the like.

Examples of the other drug for complementation and/or enhancement of thepreventive and/or therapeutic effects against epilepsia of the compoundof the present invention include phenyloin, trimethadione, ethosuximide,carbamazepine, phenobarbitone, primidone, acetazolamide, sultiame,sodium valproate, clonazepam, diazepam, nitrazepam and the like.

Examples of the other drug for complementation and/or enhancement of thepreventive and/or therapeutic effects against arteriosclerosis of thecompound of the present invention include HMG-CoA reductase inhibitor,fibrates, probucol preparation, anion-exchange resin, EPA preparation,nicotinic acid preparation, MTP inhibitor, other anti-high cholesterolagent, EDG-2 antagonist and the like.

Examples of the other drug for complementation and/or enhancement of theeffects when the compound of the present invention is used in aregeneration therapy include cytokines and various growth factors, forexample, various CSFs (for example, G-CSF, GM-CSF, etc.), variousinterleukins (for example, IL-3, 6, 7, 11, 12, etc.), EPO, TPO, SCF,FLT3 ligand, MIP-1α and the like.

Examples of the other drug for complementation and/or enhancement of thepreventive and/or therapeutic effects against retinopathy of thecompound of the present invention include antiangiogenic agent (forexample, bevacizumab, pegaptanib, SU-6668, vatalanib, ranibizumab,sorafenib, SU-11248, neovastat, etc.) and the like.

The compound of the present invention is safe and has low toxicity andtherefore can be administered to human and mammal other than human (forexample, rat, mouse, rabbit, sheep, pig, cow, cat, dog, monkey, etc.).

In order to use a pharmaceutical composition comprising the compound ofthe present invention or a concomitant drug of the compound of thepresent invention and other drugs, it is commonly administered,systematically or locally, in an oral or parenteral dosage form.

The dosage of the pharmaceutical preparation varies depending on theage, body weight, symptom, the desired therapeutic effect, the route ofadministration and duration of treatment. For the human adult, thedosage per person is between 0.1 mg and 1000 mg, by oral administration,up to several times per day, between 0.01 mg and 100 mg, by parenteraladministration, or continuous administration 1 hour to 24 hours per dayfrom vein.

As a matter of course, since the dosage varies under various conditionsas is described above, the dosage may be sometimes sufficient which issmaller than the above range, or sometimes the dosage must be more thanthe above range.

In case of administering a pharmaceutical composition comprising thecompound of the present invention, or a concomitant drug of the compoundof the present invention and other drugs, it is used as solidpreparations for internal use and solutions for internal use for oraladministration, and injections, external preparations, suppositories,ophthalmic solutions, nasal drops, inhalants and the like for parenteraladministration.

Examples of the solid preparation for internal use for oraladministration include tablets, pills, capsules, powders, and granules.Capsules include hard capsules and soft capsules.

In such a solid preparation for internal use, one or more activesubstances are used as they are, or used after mixing with excipients(lactose, mannitol, glucose, microcrystalline cellulose, starch, etc.),binders (hydroxypropyl cellulose, polyvinyl pyrrolidone, magnesiumaluminometasilicate, etc.), disintegrants (calcium carboxymethylcellulose, etc.), lubricants (magnesium stearate, etc.), stabilizers andsolubilizing agents (glutamic acid, aspartic acid, etc.) and forminginto a preparation according to a conventional method. If necessary, thepreparation may be coated with a coating agent (saccharose, gelatin,hydroxypropyl cellulose, hydroxypropylmethyl cellulosephthalate, etc.)or may be coated with two or more layers. Furthermore, capsules made ofan absorbable substance such as gelatin is included.

The solutions for internal use for oral administration includepharmaceutically acceptable water, suspensions, emulsions, syrups, andelixirs. In such a solution, one or more active substances aredissolved, suspended or emulsified in a diluent used commonly (purifiedwater, ethanol, mixed solution thereof, etc.). Furthermore, thissolution may contain humectants, suspending agents, emulsifiers,sweeteners, flavors, aromatics, preservatives, buffers, and the like.

The dosage form of the external preparation for parenteraladministration includes, for example, ointment, gel, cream, fomentation,patch, liniment, propellant, inhalant, spray, aerosol, ophthalmicsolution, and nasal drop. These products contain one or more activesubstances and are prepared according to the formulation which is knownor commonly used.

An ointment is prepared in accordance with a well known formulation or acommonly employed formulation. For example, it is prepared bytriturating or dissolving one or more active substances in a base. Anointment base is selected from well known ones or those commonlyemployed. For example, those selected from higher fatty acids or higherfatty acid esters (adipic acid, myristic acid, palmitic acid, stearicacid, oleic acid, adipate ester, myristate ester, palmitate ester,stearate ester, oleate ester, etc.), waxes (beeswax, whale wax, ceresin,etc.), surfactants (polyoxyethylene alkyl ether phosphate ester, etc.),higher alcohols (cetanol, stearyl alcohol, cetostearyl alcohol, etc.),silicone oils (dimethylpolysiloxane, etc.), hydrocarbons (hydrophilicpetrolatum, white petrolatum, purified lanolin, liquid paraffin, etc.),glycols (ethylene glycol, diethylene glycol, propylene glycol,polyethylene glycol, macrogol, etc.), vegetable oils (castor oil, oliveoil, sesame oil, turpentine oil, etc.), animal oils (mink oil, egg yolkoil, squalane, squalene, etc.), water, absorption accelerators, agentsfor preventing contact dermatitis are used alone or in combination.Furthermore, it may contain humectants, preservatives, stabilizers,antioxidizing agents, flavors, and the like.

A gel is prepared according to the formulation which is known orcommonly used. For example, it is prepared by dissolving one or moreactive substances in a base. A gel base is selected from a base which isknown or commonly used. For example, those selected from lower alcohols(ethanol, isopropyl alcohol, etc.), gelling agents (carboxymethylcellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, ethylcellulose, etc.), neutralizers (triethanolamine, diisopropanolamine,etc.), surfactants (monostearic acid polyethylene glycol, etc.), gums,water, absorption accelerator, and agent for preventing contactdermatitis are used alone or in combination. Furthermore, it may containpreservatives, antioxidizing agents, and flavoring agent.

A cream is prepared according to the formulation which is known orcommonly used. For example, it is prepared by dissolving or emulsifyingone or more active substances in a base. A cream base is selected from abase which is known or commonly used. For example, those selected fromhigher fatty acid esters, lower alcohols, hydrocarbons, polyhydricalcohols (propylene glycol, 1,3-butylene glycol, etc.), higher alcohols(2-hexyl decanol, cetanol, etc.), emulsifiers (polyoxyethylene alkylethers, fatty acid esters, etc.), water, absorption accelerators, andagents for preventing contact dermatitis are used alone or incombination. Furthermore, it may contain preservatives, antioxidizingagents, and flavoring agents.

A fomentation is prepared according to the formulation which is known orcommonly used. For example, it is prepared by dissolving one or moreactive substances in a base to obtain a kneaded mixture and spreadingthe kneaded mixture over a substrate. A fomentation base is selectedfrom a base which is known or commonly used. For example, those selectedfrom thickeners (polyacrylic acid, polyvinyl pyrrolidone, gum arabic,starch, gelatin, methyl cellulose, etc.), humectants (urea, glycerin,propylene glycol, etc.), fillers (kaolin, zinc oxide, talc, calcium,magnesium, etc.), water, solubilizing agents, tackifiers, and agents forpreventing contact dermatitis are used alone or in combination.Furthermore, it may contain preservatives, antioxidizing agents, andflavoring agent.

A patch is prepared according to the formulation which is known orcommonly used. For example, it is prepared by dissolving one or moreactive substances in a base, and spreading the solution over asubstrate. A patch base is selected from a base which is known orcommonly used. For example, those selected from polymer bases, fats andoils, higher fatty acids, tackifiers, and agents for preventing contactdermatitis are used alone or in combination. Furthermore, it may containpreservatives, antioxidizing agents, and flavoring agent.

A liniment is prepared according to the formulation which is known orcommonly used. For example, it is prepared by dissolving, suspending oremulsifying one or more active substances in one or more kinds selectedfrom water, alcohol (ethanol, polyethylene glycol, etc.), higher fattyacid, glycerin, soap, emulsifier, and suspending agent. Furthermore, itmay contain preservatives, antioxidizing agents, and flavoring agent.

A propellant, an inhalant, and a spray may contain, in addition to adiluent used commonly, a stabilizer such as sodium hydrogensulfite and abuffer capable of imparting isotonicity, for example, an isotonicitysuch as sodium chloride, sodium citrate or citric acid. The method forproducing a spray is described in detail in U.S. Pat. No. 2,868,691 andU.S. Pat. No. 3,095,355.

An injection for parenteral administration includes all injections andalso includes a drop. For example, it includes intramuscular injection,subcutaneous injection, endodermic injection, intraarterial injection,intravenous injection, intraperitoneal injection, intraspinal injection,and intravenous drop.

The injection for parenteral administration includes solutions,suspensions, emulsions, and solid injections used by dissolving orsuspending in a solvent before use. The injection is used afterdissolving, suspending, or emulsifying one or more active substances ina solvent. As the solvent, for example, distilled water for injection,physiological saline, vegetable oil, and alcohols such as propyleneglycol, polyethylene glycol or ethanol are used alone or in combination.Furthermore, the injection may contain stabilizers, solubilizing agents(glutamic acid, aspartic acid, polysolvate 80®, etc.), suspendingagents, emulsifiers, soothing agents, buffers, and preservatives. Theseinjections are prepared by sterilizing in the final process, or preparedby an aseptic treatment. Also, a sterile solid, for example, afreeze-dried product is prepared and can be used after dissolving insterilized distilled water or distilled water for sterile injection, orthe other solvent before use.

An ophthalmic solution for parenteral administration includes ophthalmicsolution, suspension type ophthalmic solution, emulsion type ophthalmicsolution, ophthalmic solution soluble when used, and eye ointment.

These ophthalmic solutions are prepared according to a known method. Forexample, one or more active substances are dissolved, suspended oremulsified in a solvent before use. As the solvent for ophthalmicsolution, for example, sterilized purified water, physiological saline,and other aqueous solvent or non-aqueous agent for injection (forexample, vegetable oil, etc.) are used alone or in combination. Ifnecessary, the ophthalmic solution may contain appropriately selectedisotonizing agents (sodium chloride, concentrated glycerin, etc.),buffering agents (sodium phosphoate, sodium acetate, etc.), surfactants(polysolvate 80 (trade name), polyoxyl 40 stearate, polyoxyethylenehardened castor oil, etc.), stabilizers (sodium citrate, sodium edetate,etc.), and antiseptics (benzalkonium chloride, paraben, etc.) Theseophthalmic solutions are prepared by sterilizing in the final process,or prepared by an aseptic treatment. Also, a sterile solid, for example,a freeze-dried product is prepared and can be used after dissolving insterilized distilled water or distilled water for sterile injection, orthe other solvent before use.

An inhalants for parenteral administration includes aerozol, inhalationpowder, and inhalation solution, and the inhalation solution may be sucha configuration that it is used after dissolving in water or othersuitable medium at the point of use.

These inhalants are prepared according to a known method.

For example, an inhalation solution is prepared by appropriatelyselecting antiseptics (benzalkonium chloride, paraben, etc.), colorants,buffering agents (sodium phosphate, sodium acetate, etc.), isotonizingagents (sodium chloride, concentrated glycerin, etc.), thickeners(carboxyvinyl polymer, etc.), and absorption accelerator, if necessary.

An inhalation powder is prepared by appropriately selecting lubricants(stearic acid and a salt thereof, etc.), binders (starch, dextrin,etc.), excipients (lactose, cellulose, etc.), colorants, antiseptics(benzalkonium chloride, paraben, etc.), and absorption accelerator ifnecessary.

In case of administering the inhalation solution, a spraying apparatus(atomizer, nebulizer) is commonly used. In case of administering theinhalation powder, an inhalation administration apparatus for powder iscommonly used.

The other composition for parenteral administration includessuppositories for intrarectal injection and pessaries for vaginaladministration, which contain one or more active substances and areformulate by a conventional method.

Designation of the compound of the present invention is described below.

The compounds used in the present invention were commonly designatedusing a computer program ACD/Name Batch® (manufactured by AdvancedChemistry Development Inc.) which designates according to the regulationof IUPAC, or commonly designated according to IUPAC Nomenclature. Forexample, a compound wherein A¹ and A² represent an imidazol-2-yl group,B¹ and B² represent a methylene group, D represents a nitrogen atom, Grepresents a carbonyl group, E represents a 1,4-phenylene group, Lrepresents —CH₂—NH—, and J represents —(CH₂)₄—N(CH₂CH₂CH₃)₂, namely, acompound represented by the following formula:

is designated as4-({[4-(dipropylamino)butyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide.

The compound of the present invention has an antagonistic activityagainst CXCR4 and is therefore useful as a preventive and/or therapeuticagent for diseases associated with CXCR4, namely, CXCR4-mediateddiseases.

BEST MODE FOR CARRYING OUT THE INVENTION

The present invention is described in detail based on Examples, but thepresent invention is not limited thereto.

Crystallinity of the solid product was confirmed using a polarizingmicroscope.

The point of separation by chromatography and the solvent in theparentheses shown in TLC indicate a dissolution medium or an eluentused, and the proportion indicates a volume ratio.

NMR is a measured value of ¹HNMR at 300 MHz and the solvent shown in theparentheses of NMR indicates a solvent used in the measurement.

Regarding MS, detection for only positive ions (pos.) was conductedusing ESI (electron spray ion) method unless otherwise specified.

HPLC conditions are as follows.

Apparatus used: Waters LC/MS®

Column: Xterra® MS C₁₈ 5 μm, 3×50 mm I.D.

Flow rate: 1.5 mL/min

Solvent:

Solution A: aqueous 0.1% trifluoroacetic acid solution

Solution B: 0.1% trifluoroacetic acid-acetonitrile solution

For one minute after starting the measurement, the mixing ratio of thesolution A to the solution B was fixed to 95:5. The mixing ratio of thesolution A to the solution B was linearly changed to 5:95 over 3 minutesafter fixation. Then, the mixing ratio of the solution A to the solutionB was fixed to 5:95 for 0.5 minute. The mixing ratio of the solution Ato the solution B was linearly changed to 95:5 for 0.01 minute afterfixation.

Example 1N-(4-methoxybenzyl)-N,N-bis[(1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-imidazol-2-yl)methyl]amine(Compound 1)

To a solution of1-({[2-(trimethylsilyl)ethyloxy]methyl}-1H-imidazole-2-carboaldehyde(CAS Registry Number: 101226-42-0, 1.5 g) and{[4-(methyloxy)phenyl]methyl}amine (0.39 mL) in dichloromethane(containing 1% acetic acid) (10 mL), sodium triacetoxyborohydride (1.91g) was added. The reaction mixture was stirred at room temperature for16 hours. The reaction mixture was added by an aqueous 1 N sodiumhydroxide solution and then extracted with dichloromethane. The extractthus obtained was washed with saturated brine, dried over anhydroussodium sulfate and then concentrated. The obtained residue was purifiedby silica gel column chromatography (ethylacetate:methanol:triethylamine=1:0:0→10:1:0→10:1:0.1) to obtain thetitle compound (1.71 g) having the following physical properties.

TLC: Rf 0.51 (chloroform:methanol: 28% ammonia water=80:10:1)

NMR (CDCl₃): δ 7.20 (d, 2H), 6.97 (m, 4H), 6.83 (d, 2H), 5.11 (s, 4H),3.82 (s, 4H), 3.78 (s, 3H), 3.60 (s, 2H), 3.24 (m, 4H), 0.77 (m, 4H),−0.05 (s, 18H)

Example 2N,N-bis[(1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-imidazol-2-yl)methyl]amine(Compound 2)

To a solution of the compound 1 (665 mg) in ethanol (5 mL), 20%palladium hydroxide carbon (500 mg) was added under an argon atmosphere.The reaction mixture was stirred under a hydrogen gas atmosphere at 65°C. for 3 hours. The reaction mixture was filtered through Celite (tradename) and then concentrated. The obtained residue was purified by silicagel column chromatography (dichloromethane:methanol: 28% ammoniawater=10:1:0→80:10:1) to obtain the title compound (467 mg) having thefollowing physical properties.

TLC: Rf 0.32 (chloroform:methanol: 28% ammonia water=80:10:1)

NMR (CDCl₃): δ 6.97 (m, 4H), 5.35 (s, 4H), 3.47 (m, 4H), 3.18 (m, 4H),0.88 (m, 4H), −0.03 (s, 18H)

Example 3 Methyl4-[({4-[(tert-butoxycarbonyl)amino]butyl}amino)methyl]benzoate (Compound3)

The same procedure as a series of reactions of Example 1 was carriedout, except that methyl 4-formylbenzoate was used in place of1-({[2-(trimethylsilyl)ethyloxy]methyl}-1H-imidazole-2-carboaldehyde and1,1-dimethylethyl (4-aminobutyl)carbamate was used in place of{[4-(methyloxy)phenyl]methyl}amine, to obtain the title compound havingthe following physical properties.

TLC: Rf 0.45 (chloroform:methanol=10:1)

NMR (CDCl₃): δ 7.99 (d, 2H), 7.39 (d, 2H), 4.77 (m, 1H), 3.91 (s, 3H),3.84 (s, 2H), 3.13 (m, 2H), 2.64 (m, 2H), 1.54 (m, 4H), 1.44 (s, 9H)

Example 4 Methyl4-[([(benzyloxy)carbonyl]{4-[(tert-butoxycarbonyl)amino]butyl}amino)methyl]benzoate(Compound 4)

To a solution of the compound 3 (810 mg) and sodium carbonate (383 mg)in water (5 mL) and tetrahydrofuran (2 mL), benzyl chloroformate (413μl) was added at 0° C. The reaction mixture was stirred at 0° C. for onehour. The reaction mixture was diluted with ethyl acetate. The organiclayer was washed with water and saturated brine, dried over anhydroussodium sulfate and then concentrated. The obtained residue was purifiedby silica gel column chromatography (hexane:ethyl acetate=4:1→3:1) toobtain the title compound (1.16 g) having the following physicalproperties.

TLC: Rf 0.27 (hexane:ethyl acetate=3:1)

NMR (CDCl₃): δ 7.97 (m, 2H), 7.27 (m, 7H), 5.17 (m, 2H), 4.53 (s, 2H),4.41 (m, 1H), 3.91 (s, 3H), 3.26 (m, 2H), 3.09 (m, 2H), 1.47 (m, 4H),1.43 (s, 9H)

Example 54-[([(benzyloxy)carbonyl]{4-[(tert-butoxycarbonyl)amino]butyl}amino)methyl]benzoicacid (Compound 5)

To a solution of the compound 4 (1.13 g) in methanol (4 mL), an aqueous2N sodium hydroxide solution (4 mL) was added. The reaction mixture wasstirred at room temperature for 3 hours. The reaction mixture wasconcentrated. The obtained residue was added by 2N hydrochloric acid andthen extracted with ethyl acetate. The extract was washed with saturatedbrine, dried over anhydrous sodium sulfate and then concentrated toobtain the title compound having the following physical properties.

TLC: Rf 0.51 (chloroform:methanol:acetic acid=90:10:5)

NMR (CDCl₃): δ 8.03 (m, 2H), 7.26 (m, 7H), 5.17 (m, 2H), 4.55 (s, 2H),4.39 (m, 1H), 3.26 (m, 2H), 3.05 (m, 2H), 1.49 (m, 4H), 1.44 (s, 9H)

Example 6Phenylmethyl({4-[(bis{[1-({[2-(trimethylsilyl)ethyl]oxy}methyl)-1H-imidazol-2-yl]methyl}amino)carbonyl]phenyl}methyl)[4-({[(1,1-dimethylethyl)oxy]carbonyl}amino)butyl]carbamate(Compound 6)

To a solution of the compound 5 (137 mg) in dichloromethane (3 mL), thecompound 2 (131 mg), 1-hydroxybenzotriazole (69 mg),1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide hydrochloride (86 mg)and diisopropylethylamine (105 μl) were added. The reaction mixture wasstirred at room temperature for 5 hours. The reaction mixture was addedby water and then extracted with dichloromethane. The extract was washedwith saturated brine, dried over anhydrous sodium sulfate and thenconcentrated. The obtained residue was purified by silica gel columnchromatography (hexane:ethylacetate=100:0→0:100→dichloromethane:methanol=10:1) to obtain the titlecompound (225 mg) having the following physical properties.

TLC: Rf 0.38 (chloroform:methanol:acetic acid=90:10:5)

NMR (CDCl₃): δ 7.33 (m, 9H), 7.16 (m, 4H), 5.55 (s, 2H), 5.15 (s, 4H),4.89 (m, 2H), 4.47 (s, 2H), 4.41 (m, 1H), 3.60 (m, 4H), 3.22 (m, 6H),1.58 (m, 4H), 1.44 (s, 9H), −0.92 (m, 4H), 0.05 (s, 18H)

Example 7 Benzyl4-aminobutyl[4-({bis[(1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-imidazol-2-yl)methyl]amino}carbonyl)benzyl]carbamate(Compound 7)

To the compound 6 (225 mg), a solution of 25% trifluoroacetic acid indichloromethane (6 mL) was added. The reaction mixture was stirred atroom temperature for one hour. The reaction mixture was concentrated andthe residue was added by 1N sodium hydroxide and then extracted withdichloromethane. The extract was washed with saturated brine, dried overanhydrous sodium sulfate and then concentrated. The obtained residue waspurified by silica gel column chromatography (dichloromethane:methanol:28% ammonia water=80:10:1) to obtain the title compound (92 mg) havingthe following physical properties.

TLC: Rf 0.31 (chloroform:methanol: 28% ammonia water=80:10:1)

NMR (CDCl₃): δ 7.32 (m, 9H), 7.14 (m, 4H), 5.51 (s, 2H), 5.16 (s, 4H),4.88 (m, 2H), 4.47 (s, 2H), 3.53 (m, 4H), 3.25 (m, 6H), 1.58 (m, 4H),0.92 (m, 4H), −0.04 (s, 18H).

Example 8 Benzyl4-({bis[(1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-imidazol-2-yl)methyl]amino}carbonyl)benzyl[4-(dipropylamino)butyl]carbamate(Compound 8)

The same procedure as a series of reactions of Example 1 was carriedout, except that 1-propanal was used in place of1-({[2-(trimethylsilyl)ethyloxy]methyl}-1H-imidazole-2-carboaldehyde andthe compound 7 was used in place of {[4-(methyloxy)phenyl]methyl}amine,to obtain the title compound having the following physical properties.

TLC: Rf 0.63 (chloroform:methanol: 28% ammonia water=80:10:1)

NMR (CDCl₃): δ 7.63 (d, J=8.1 Hz, 2H), 7.29 (m, 7H), 7.02 (m, 4H), 5.51(m, 2H), 5.17 (s, 4H), 4.84 (m, 2H), 4.66 (s, 2H), 4.47 (s, 2H), 3.53(m, 2H), 3.35 (m, 2H), 3.24 (m, 2H), 2.58 (m, 6H), 1.55 (m, 8H), 0.92(m, 6H), 0.76 (m, 4H), 0.03 (s, 18H).

Example 94-({[4-(dipropylamino)butyl]amino}methyl)-N,N-bis[(1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-imidazol-2-yl)methyl]benzamide(Compound 9)

The same procedure as a series of reactions of Example 2 was carriedout, except that the compound 8 was used in place of the compound 1 and10% palladium carbon was used in place of 20% palladium hydroxidecarbon, to obtain the title compound having the following physicalproperties.

TLC: Rf 0.45 (chloroform:methanol: 28% ammonia water=80:10:1)

Example 104-({[4-(dipropylamino)butyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 10)

To a solution of the compound 9 in methanol (2 mL), concentratedhydrochloric acid (2 mL) was added. The reaction mixture was stirred at80° C. for 5 hours. The reaction mixture was added by an aqueous 5Nsodium hydroxide solution and then extracted with dichloromethane. Theextract was washed with saturated brine, dried over anhydrous sodiumsulfate and then concentrated. The obtained residue was purified bysilica gel column chromatography (dichloromethane:methanol: 28% ammoniawater=80:10:1→80:20:4) to obtain the title compound (21 mg) having thefollowing physical properties.

TLC: Rf 0.67 (chloroform:methanol: 28% ammonia water=80:20:4)

NMR (DMSO-d₆): δ 0.80 (t, J=7.20 Hz, 6H), 1.18-1.49 (m, 8H), 2.18-2.36(m, 6H), 2.39-2.47 (m, 2H), 3.68 (s, 2H), 4.47-4.68 (m, 4H), 6.78-7.18(m, 4H), 7.32 (d, J=7.80 Hz, 2H), 7.42 (d, J=7.80 Hz, 2H), 11.85-12.96(m, 2H)

Example 114-({[3-(dibutylamino)propyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 11)

The same procedure as a series of reactions of Example 1 was carriedout, except that methyl 4-formylbenzoate was used in place of1-({[2-(trimethylsilyl)ethyloxy]methyl}-1H-imidazole-2-carboaldehyde andN,N-dibutyl-1,3-propanediamine was used in place of{[4-(methyloxy)phenyl]methyl}amine, to obtain methyl4-({[3-(dibutylamino)propyl]amino}methyl)benzoate (compound 11-a). Thesame procedure as a series of reactions of Example 4→Example 5→Example6→Example 9→Example 10 was carried out, except that the compound 11-awas used in place of the compound 3 in the process of Example 4, toobtain the title compound having the following physical properties.

Description: oily product

TLC: Rf 0.51 (dichloromethane:methanol: 28% ammonia water=80:20:3)

NMR (CDCl₃): δ 0.89 (t, J=7.2 Hz, 6H), 1.16-1.46 (m, 8H), 1.55-1.72 (m,2H), 2.31-2.50 (m, 6H), 2.64 (t, J=6.9 Hz, 2H), 3.78 (s, 2H), 4.51-4.77(m, 4H), 6.89-7.11 (m, 4H), 7.35 (d, J=8.1 Hz, 2H), 7.55 (d, J=8.1 Hz,2H)

Example 11(1) to Example 11(21)

The same procedure as a series of reactions of Example 11 was carriedout, except that a corresponding amine compound was used in place ofN,N-dibutyl-1,3-propanediamine, to obtain the following compound of thepresent invention.

Example 11(1)4-({[4-(dipropylamino)-4-oxobutyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 11-1)

Description: amorphous

TLC: Rf 0.58 (dichloromethane:methanol: 28% ammonia water=80:20:3);

NMR (CDCl₃): δ 0.77-0.98 (m, 6H), 1.40-1.64 (m, 4H), 1.72-1.91 (m, 2H),2.36 (t, J=7.2 Hz, 2H), 2.68 (t, J=7.2 Hz, 2H), 3.08-3.32 (m, 4H), 3.81(s, 2H), 4.45-4.80 (m, 4H), 6.92-7.10 (m, 4H), 7.33 (d, J=8.1 Hz, 2H),7.48 (d, J=8.1 Hz, 2H).

Example 11(2)4-({[5-(dipropylamino)pentyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 11-2)

Description: amorphous

TLC: Rf 0.52 (dichloromethane:methanol: 28% ammonia water=80:20:3)

NMR (CDCl₃): δ 0.86 (t, J=7.5 Hz, 6H), 1.16-1.64 (m, 10H), 2.26-2.48 (m,6H), 2.60 (t, J=7.2 Hz, 2H), 3.78 (s, 2H), 4.48-4.78 (m, 4H), 6.85-7.10(m, 4H), 7.33 (d, J=8.1 Hz, 2H), 7.52 (d, J=8.1 Hz, 2H)

Example 11(3)4-({4-[(dipropylamino)methyl]-1-piperidinyl}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 11-3)

Description: amorphous

TLC: Rf 0.40 (methanol: 28% ammonia water=99:1);

NMR (CDCl₃): δ 0.85 (t, J=7.5 Hz, 6H), 1.04-1.26 (m, 2H), 1.29-1.50 (m,5H), 1.62-1.80 (m, 2H), 1.83-2.02 (m, 2H), 2.18 (d, J=6.9 Hz, 2H),2.23-2.37 (m, 4H), 2.72-2.90 (m, 2H), 3.48 (s, 2H), 4.55-4.78 (m, 4H),6.87-7.12 (m, 4H), 7.35 (d, J=8.1 Hz, 2H), 7.57 (d, J=8.1 Hz, 2H).

Example 11(4)4-({[4-(dipropylamino)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 11-4)

Description: amorphous

TLC: Rf 0.22 (methanol: 28% ammonia water=99:1)

NMR (CDCl₃): δ 0.77-0.92 (m, 6H), 0.99-2.06 (m, 12H), 2.28-2.56 (m, 6H),3.69-3.89 (m, 2H), 4.52-4.78 (m, 4H), 6.91-7.15 (m, 4H), 7.29-7.45 (m,2H), 7.48-7.64 (m, 2H)

Example 11(5)N,N-bis(1H-imidazol-2-ylmethyl)-4-[(2-propyl-2,9-diazaspiro[5.5]undec-9-yl)methyl]benzamide(Compound 11-5)

Description: amorphous

TLC: Rf 0.32 (methanol: 28% ammonia water=99:1)

NMR (CDCl₃): δ 0.87 (t, J=7.5 Hz, 3H), 1.20-1.34 (m, 2H), 1.36-1.64 (m,8H), 2.01-2.45 (m, 10H), 3.41-3.54 (m, 2H), 4.56-4.77 (m, 4H), 6.89-7.13(m, 4H), 7.35 (d, J=8.1 Hz, 2H), 7.54 (d, J=8.1 Hz, 2H)

Example 11(6)N,N-bis(1H-imidazol-2-ylmethyl)-4-[(2-propyl-2,8-diazaspiro[4.5]dec-8-yl)methyl]benzamide(Compound 11-6)

Description: amorphous

TLC: Rf 0.13 (methanol: 28% ammonia water=99:1);

NMR (CDCl₃): δ 0.89 (t, J=7.5 Hz, 3H), 1.40-1.69 (m, 8H), 2.23-2.44 (m,8H), 2.57 (t, J=6.9 Hz, 2H), 3.46 (s, 2H), 4.55-4.77 (m, 4H), 6.86-7.14(m, 4H), 7.35 (d, J=8.1 Hz, 2H), 7.56 (d, J=8.1 Hz, 2H)

Example 11(7)N,N-bis(1H-imidazol-2-ylmethyl)-4-[(4-propyl-1,4-diazepan-1-yl)methyl]benzamide(Compound 11-7)

Description: amorphous

TLC: Rf 0.22 (methanol: 28% ammonia water=99:1);

NMR (CDCl₃): δ 0.87 (t, J=7.5 Hz, 2H), 1.32-1.60 (m, 3H), 1.68-1.85 (m,2H), 2.34-2.50 (m, 2H), 2.56-2.80 (m, 8H), 3.62 (s, 2H), 4.52-4.80 (m,4H), 6.85-7.13 (m, 4H), 7.37 (d, J=8.1 Hz, 2H), 7.54 (d, J=8.1 Hz, 2H)

Example 11(8)4-{[(3S)-1-butyl-3-isobutyl-2,5-dioxo-1,4,9-triazaspiro[5.5]undec-9-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 11-8)

Description: amorphous

TLC: Rf 0.69 (methanol: 28% ammonia water=99:1)

NMR (CDCl₃): δ 0.83-1.06 (m, 9H), 1.21-2.23 (m, 11H), 2.47-2.96 (m, 4H),3.25-3.50 (m, 2H), 3.56 (s, 2H), 3.86-4.02 (m, 1H), 4.54-4.78 (m, 4H),6.09-6.29 (m, 1H), 6.90-7.12 (m, 4H), 7.37 (d, J=8.1 Hz, 2H), 7.58 (d,J=8.1 Hz, 2H)

Example 11(9)N,N-bis(1H-imidazol-2-ylmethyl)-4-(1-piperidinylmethyl)benzamide(Compound 11-9)

Description: amorphous

TLC: Rf 0.51 (methanol: 28% ammonia water=99:1)

NMR (CDCl₃): δ 1.33-1.48 (m, 2H), 1.48-1.63 (m, 4H), 2.24-2.45 (m, 4H),3.46 (s, 2H), 4.54-4.76 (m, 4H), 6.95-7.11 (m, 4H), 7.36 (d, J=8.1 Hz,2H), 7.58 (d, J=8.1 Hz, 2H)

Example 11(10)4-({[3-(dipropylamino)propyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 11-10)

Description: amorphous

TLC: Rf 0.28 (methanol: 28% ammonia water=99:1);

NMR (CDCl₃): δ 0.84 (t, J=7.5 Hz, 6H), 1.31-1.52 (m, 4H), 1.53-1.74 (m,2H), 2.26-2.39 (m, 4H), 2.45 (t, J=6.86 Hz, 2H), 2.64 (t, J=6.9 Hz, 2H),3.77 (s, 2H), 4.50-4.79 (m, 4H), 6.84-7.13 (m, 4H), 7.32 (d, J=8.1 Hz,2H), 7.51 (d, J=8.1 Hz, 2H)

Example 11(11)4-({[2-(dipropylamino)ethyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 11-11)

Description: amorphous

TLC: Rf 0.42 (methanol: 28% ammonia water=99:1)

NMR (CDCl₃): δ 0.84 (t, J=7.5 Hz, 6H), 1.30-1.53 (m, 4H), 2.25-2.41 (m,4H), 2.48-2.58 (m, 2H), 2.59-2.70 (m, 2H), 3.80 (s, 2H), 4.50-4.79 (m,4H), 6.86-7.14 (m, 4H), 7.34 (d, J=8.1 Hz, 2H), 7.56 (d, J=8.1 Hz, 2H)

Example 11(12)4-{[7-(dipropylamino)-3,4-dihydro-2(1H)-isoquinolinyl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 11-12)

Description: amorphous

TLC: Rf 0.54 (methanol: 28% ammonia water=99:1)

NMR (CDCl₃): δ 0.89 (t, J=7.5 Hz, 6H), 1.42-1.68 (m, 4H), 2.61-2.87 (m,4H), 3.06-3.25 (m, 4H), 3.54 (s, 2H), 3.67 (s, 2H), 4.56-4.77 (m, 4H),6.23 (d, J=2.7 Hz, 1H), 6.48 (dd, J=8.7, 2.4 Hz, 1H), 6.93 (d, J=8.7 Hz,1H), 6.96-7.13 (m, 4H), 7.45 (d, J=8.1 Hz, 2H), 7.59 (d, J=8.1 Hz, 2H)

Example 11(13)N,N-bis(1H-imidazol-2-ylmethyl)-4-[(9-propyl-3,9-diazaspiro[5.5]undec-3-yl)methyl]benzamide(Compound 11-13)

Description: amorphous

TLC: Rf 0.61 (dichloromethane:methanol: 28% ammonia water=80:20:3);

NMR (CDCl₃): δ 0.88 (t, J=7.5 Hz, 3H), 1.33-1.66 (m, 10H), 2.16-2.49 (m,10H), 3.50 (s, 2H), 4.54-4.79 (m, 4H), 6.91-7.12 (m, 4H), 7.35 (d, J=8.1Hz, 2H), 7.55 (d, J=8.1 Hz, 2H)

Example 11(14)N,N-bis(1H-imidazol-2-ylmethyl)-4-[(9-propyl-2,9-diazaspiro[5.5]undec-2-yl)methyl]benzamide(Compound 11-14)

Description: amorphous

TLC: Rf 0.64 (dichloromethane:methanol: 28% ammonia water=80:20:3)

NMR (CDCl₃): δ 0.85 (t, J=7.5 Hz, 3H), 1.21-1.68 (m, 10H), 1.99-2.49 (m,10H), 3.42 (s, 2H), 4.51-4.80 (m, 4H), 6.87-7.15 (m, 4H), 7.35 (d, J=8.1Hz, 2H), 7.55 (d, J=8.1 Hz, 2H)

Example 11(15)N,N-bis(1H-imidazol-2-ylmethyl)-4-({[(1-propyl-4-piperidinyl)methyl]amino}methyl)benzamide(Compound 11-15)

Description: amorphous

TLC: Rf 0.15 (dichloromethane:methanol: 28% ammonia water=80:20:3)

NMR (CDCl₃): δ 0.88 (t, J=7.32 Hz, 3H), 1.14-1.35 (m, 2H), 1.37-1.60 (m,3H), 1.63-1.79 (m, 2H), 1.81-1.98 (m, 2H), 2.19-2.34 (m, 2H), 2.48 (d,J=6.77 Hz, 2H), 2.84-3.02 (m, 2H), 3.79 (s, 2H), 4.51-4.76 (m, 4H),6.91-7.16 (m, 4H), 7.37 (d, J=8.1 Hz, 2H), 7.63 (d, J=8.1 Hz, 2H)

Example 11(16)4-({[4-(dipropylamino)phenyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 11-16)

Description: dark green amorphous

TLC: Rf 0.64 (dichloromethane:methanol: 28% ammonia water=80:20:3)

NMR (CDCl₃): δ 0.70-1.06 (m, 6H), 1.31-1.74 (m, 4H), 2.68-3.50 (m, 4H),4.01-4.57 (m, 2H), 4.57-4.84 (m, 4H), 6.22-6.89 (m, 4H), 6.90-7.14 (m,4H), 7.41 (d, J=8.1 Hz, 2H), 7.60 (d, J=8.1 Hz, 2H)

Example 11(17)4-[(2-benzyl-2,9-diazaspiro[5.5]undec-9-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 11-17)

Description: amorphous

TLC: Rf 0.67 (dichloromethane:methanol: 28% ammonia water=80:20:1)

NMR (CDCl₃): δ 1.21-1.38 (m, 2H), 1.40-1.66 (m, 6H), 1.99-2.44 (m, 8H),3.34-3.47 (m, 4H), 4.53-4.81 (m, 4H), 6.92-7.12 (m, 4H), 7.19-7.30 (m,5H), 7.32 (d, J=8.1 Hz, 2H), 7.52 (d, J=8.1 Hz, 2H)

Example 11(18)4-[(2-cyclohexyl-2,9-diazaspiro[5.5]undec-9-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 11-18)

Description: amorphous

TLC: Rf 0.40 (dichloromethane:methanol: 28% ammonia water=80:20:1)

NMR (CDCl₃): δ 1.08-1.84 (m, 18H), 2.13-2.54 (m, 9H), 3.48 (s, 2H),4.57-4.77 (m, 4H), 6.93-7.13 (m, 4H), 7.36 (d, J=8.1 Hz, 2H), 7.53 (d,J=8.1 Hz, 2H)

Example 11(19)4-{[(3S)-1-butyl-3-isobutyl-5-oxo-1,4,9-triazaspiro[5.5]undec-9-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 11-19)

Description: amorphous

TLC: Rf 0.60 (dichloromethane:methanol: 28% ammonia water=80:20:1)

NMR (CDCl₃): δ 0.81-1.03 (m, 9H), 1.13-1.53 (m, 6H), 1.56-1.73 (m, 1H),1.75-3.06 (m, 12H), 3.56 (s, 2H), 3.59-3.73 (m, 1H), 4.51-4.84 (m, 4H),5.66 (s, 1H), 6.91-7.10 (m, 4H), 7.36 (d, J=8.1 Hz, 2H), 7.49 (d, J=8.1Hz, 2H)

Example 11(20)4-{[(3S)-1-butyl-3-isobutyl-2-oxo-4-propyl-1,4,9-triazaspiro[5.5]undec-9-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 11-20)

Description: amorphous

TLC: Rf 0.69 (dichloromethane:methanol: 28% ammonia water=80:20:1)

NMR (CDCl₃): δ 0.75-1.02 (m, 12H), 1.19-2.22 (m, 17H), 2.45-2.67 (m,1H), 2.67-2.84 (m, 2H), 2.86-2.98 (m, 1H), 2.98-3.16 (m, 1H), 3.16-3.27(m, 1H), 3.28-3.43 (m, 1H), 3.50 (s, 2H), 4.54-4.81 (m, 4H), 6.93-7.12(m, 4H), 7.37 (d, J=8.1 Hz, 2H), 7.58 (d, J=8.1 Hz, 2H)

Example 11(21)4-{[(3S)-1-butyl-3-isobutyl-4-propyl-1,4,9-triazaspiro[5.5]undec-9-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 11-21)

Description: amorphous

TLC: Rf 0.67 (dichloromethane:methanol: 28% ammonia water=80:20:1)

NMR (CDCl₃): δ 0.78-1.00 (m, 12H), 1.10-1.69 (m, 11H), 1.78-2.35 (m,9H), 2.49-2.82 (m, 5H), 2.93-3.09 (m, 1H), 3.37-3.58 (m, 2H), 4.51-4.82(m, 4H), 6.91-7.14 (m, 4H), 7.36 (d, J=8.1 Hz, 2H), 7.55 (d, J=8.1 Hz,2H)

Example 12 Benzyl6-({bis[(1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-imidazol-2-yl)methyl]amino}carbonyl)-3,4-dihydro-2(1H)-isoquinolinecarboxylate(Compound 12)

The same procedure as a series of reactions of Example 6 was carriedout, except that2-[(benzyloxy)carbonyl]-1,2,3,4-tetrahydro-6-isoquinolinecarboxylic acidwas use in place of the compound 2 and the compound 5, to obtain thetitle compound having the following physical properties.

TLC: Rf 0.34 (chloroform:methanol=10:1)

NMR (CDCl₃): δ 7.37 (m, 6H), 7.00 (m, 6H), 5.55 (m, 2H), 5.21 (m, 2H),5.17 (s, 2H), 4.87 (m, 4H), 4.62 (s, 2H), 3.69 (t, J=5.7 Hz, 2H), 3.53(m, 4H), 3.33 (m, 2H), 0.88 (m, 2H), 0.74 (m, 2H), −0.05 (s, 18H)

Example 13N,N-bis[(1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-imidazol-2-yl)methyl]-1,2,3,4-tetrahydro-6-isoquinolinecarboxamide(Compound 13)

The same procedure as a series of reactions of Example 2 was carriedout, except that the compound 12 was used in place of the compound 1 and10% palladium carbon was used in place of 20% palladium hydroxidecarbon, to obtain the title compound having the following physicalproperties.

TLC: Rf 0.50 (chloroform:methanol: 28% ammonia water=80:10:1)

NMR (CDCl₃): δ 7.43 (d, J=6.0 Hz, 1H), 7.36 (s, 1H), 6.99 (m, 5H), 5.50(m, 2H), 5.15 (m, 2H), 4.84 (m, 2H), 4.69 (m, 2H), 4.04 (s, 2H), 3.53(m, 2H), 3.52 (m, 2H), 3.15 (t, J=5.7 Hz, 2H), 2.81 (m, 2H), 0.77 (m,2H), −0.05 (s, 18H)

Example 142-[4-(dipropylamino)butyl]-N,N-bis[(1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-imidazol-2-yl)methyl]-1,2,3,4-tetrahydro-6-isoquinolinecarboxamide(Compound 14)

The same procedure as a series of reactions of Example 1 was carriedout, except that 4-(dipropylamino)butanal was used in place of1-({[2-(trimethylsilyl)ethyloxy]methyl}-1H-imidazole-2-carboaldehyde andthe compound 13 was used in place of {[4-(methyloxy)phenyl]methyl}amine,to obtain the title compound having the following physical properties.

TLC: Rf 0.39 (chloroform:methanol: 28% ammonia water=80:10:1)

NMR (CDCl₃): δ 7.38 (m, 2H), 6.99 (m, 5H), 5.50 (s, 2H), 5.15 (sm, 2H),4.84 (s, 2H), 4.68 (s, 2H), 3.58 (m, 4H), 3.33 (m, 2H), 2.84 (t, J=5.7Hz, 2H), 2.70 (t, J=5.7 Hz, 2H), 2.51 (m, 4H), 1.60 (m, 12H), 0.90 (m,10H), −0.05 (s, 18H)

Example 152-[4-(dipropylamino)butyl]-N,N-bis(1H-imidazol-2-ylmethyl)-1,2,3,4-tetrahydro-6-isoquinolinecarboxamide(Compound 15)

The same procedure as a series of reactions of Example 10 was carriedout, except that the compound 14 was used in place of the compound 9, toobtain the title compound having the following physical properties.

Description: amorphous

TLC: Rf 0.67 (chloroform:methanol: 28% ammonia water=80:20:4)

NMR (DMSO-d₆): δ 0.81 (t, J=7.20 Hz, 6H), 1.27-1.43 (m, 6H), 1.43-1.59(m, 2H), 2.22-2.31 (m, 4H), 2.34 (t, J=6.9 Hz, 2H), 2.41 (t, J=7.20 Hz,2H), 2.59 (t, J=6.3 Hz, 2H), 2.72 (t, J=6.30 Hz, 2H), 3.50 (s, 2H),4.43-4.57 (m, 2H), 4.57-4.71 (m, 2H), 6.75-6.98 (m, 2H), 6.99-7.16 (m,4H), 7.20 (dd, J=7.8, 1.5 Hz, 1H), 12.03-12.57 (m, 2H)

Example 15(1) to Example 15(3)

The same procedure as a series of reactions of Example 12→Example13→Example 14 and, if necessary, Example 15 was carried out, except thatthe compound 2 was used or bis[(1-methyl-1H-imidazol-2-yl)methyl]amineor bis(2-pyridinylmethyl)amine was used in place of it, and2-[(benzyloxy)carbonyl]-1,2,3,4-tetrahydro-6-isoquinolinecarboxylic acidwas used or2-[(benzyloxy)carbonyl]-1,2,3,4-tetrahydro-7-isoquinolinecarboxylic acidwas used in place of it in the process of Example 12, to obtain thefollowing compound of the present invention.

Example 15(1)2-[4-(dipropylamino)butyl]-N,N-bis(1H-imidazol-2-ylmethyl)-1,2,3,4-tetrahydro-7-isoquinolinecarboxamide(Compound 15-1)

Description: amorphous;

TLC: Rf 0.30 (chloroform:methanol: 28% ammonia water=80:10:1);

NMR (DMSO-d₆): δ 0.81 (t, J=7.2 Hz, 6H), 1.27-1.45 (m, 6H), 1.45-1.58(m, 2H), 2.22-2.31 (m, 4H), 2.32-2.37 (m, 2H), 2.37-2.45 (m, 2H), 2.60(t, J=5.4 Hz, 2H), 2.78 (t, J=5.4 Hz, 2H), 3.43 (s, 2H), 4.45-4.70 (m,4H), 6.72-6.99 (m, 2H), 6.99-7.15 (m, 3H), 7.16-7.31 (m, 2H),11.94-12.28 (m, 1H), 12.28-12.63 (m, 1H)

Example 15(2)2-[4-(dipropylamino)butyl]-N,N-bis[(1-methyl-1H-imidazol-2-yl)methyl]-1,2,3,4-tetrahydro-6-isoquinolinecarboxamide(Compound 15-2)

Description: oily product;

TLC: Rf 0.33 (chloroform:methanol: 28% ammonia water=80:10:1);

NMR (DMSO-d₆): δ 0.81 (t, J=7.5 Hz, 6H), 1.25-1.43 (m, 6H), 1.44-1.59(m, 2H), 2.22-2.31 (m, 4H), 2.34 (t, J=6.0 Hz, 2H), 2.38-2.46 (m, 2H),2.59 (t, J=5.7 Hz, 2H), 2.67-2.82 (m, 2H), 3.14-3.43 (m, 3H), 3.50 (s,2H), 3.53-3.75 (m, 3H), 4.30-4.51 (m, 2H), 4.52-4.75 (m, 2H), 6.74-6.91(m, 2H), 6.97-7.13 (m, 3H), 7.15-7.23 (m, 1H), 7.28 (d, J=8.1 Hz, 1H)

Example 15(3)2-[4-(dipropylamino)butyl]-N,N-bis(2-pyridinylmethyl)-1,2,3,4-tetrahydro-6-isoquinolinecarboxamide(Compound 15-3)

Description: oily product;

TLC: Rf 0.48 (chloroform:methanol: 28% ammonia water=80:10:1);

NMR (DMSO-d₆): δ 0.70-0.91 (m, 6H), 1.21-1.42 (m, 6H), 1.43-1.59 (m,2H), 2.17-2.45 (m, 8H), 2.55-2.66 (m, 2H), 2.68-2.80 (m, 2H), 3.43-3.59(m, 2H), 4.60 (s, 2H), 4.67 (s, 2H), 6.99-7.09 (m, 1H), 7.16-7.25 (m,3H), 7.25-7.32 (m, 2H), 7.36 (d, J=8.4 Hz, 1H), 7.65-7.93 (m, 2H),8.42-8.72 (m, 2H)

Example 161-[4-(diethoxymethyl)phenyl]-N-[(1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-imidazol-2-yl)methyl]methaneamine(Compound 16)

The same procedure as a series of reactions of Example 1, except that4-(diethoxymethyl)benzaldehyde was used in place of1-({[2-(trimethylsilyl)ethyloxy]methyl}-1H-imidazole-2-carboaldehyde and1-(1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-imidazol-2-yl)methaneaminewas used in place of {[4-(methyloxy)phenyl]methyl}amine, to obtain thetitle compound having the following physical properties.

TLC: Rf 0.63 (chloroform:methanol: 28% ammonia water=80:10:1)

NMR (CDCl₃): δ 7.43 (d, J=7.8 Hz, 2H), 7.33 (J=7.8 Hz, 2H), 6.97 (m,2H), 5.49 (s, 1H), 5.32 (s, 2H), 3.94 (s, 2H), 3.84 (s, 2H), 3.52 (m,6H), 1.24 (m, 6H), 0.88 (m, 2H), −0.03 (s, 9H)

Example 17N-[4-(diethoxymethyl)benzyl]-N-[(1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-imidazol-2-yl)methyl]-1H-imidazole-2-carboxamide(Compound 17)

To a solution of the compound 16 (1.93 g), 1H-imidazole-2-carboxylicacid (773 mg) and diisopropylethylamine (2.97 g) in dichloromethane (15mL), O-(7-azabenzotriazol-1-yl)N,N,N′,N′-tetramethyluroniumhexafluorophosphate (HATU) (2.62 g) was added under ice cooling. Thereaction mixture was stirred at room temperature for 4 hours. To thereaction mixture, an aqueous 1N sodium hydroxide solution (30 mL) wasadded. The aqueous layer was extracted twice with dichloromethane (50mL). The combined organic layer was washed with saturated brine, driedover anhydrous magnesium sulfate and then concentrated. The residue waspurified by silica gel chromatography (ethyl acetate:methanol=1:0→10:1)to obtain the title compound (977 mg) having the following physicalproperties.

TLC: Rf 0.44 (chloroform:methanol: 28% ammonia water=80:10:1);

NMR (CDCl₃): δ 7.41 (m, 4H), 7.15 (s, 2H), 7.04 (s, 1H), 6.96 (m, 1H),5.82 (s, 1H), 5.77 (s, 1H), 5.46 (s, 1H), 5.37 (s, 1H), 5.23 (s, 1H),4.83 (s, 1H), 4.76 (s, 1H), 3.51 (m, 6H), 1.25 (m, 6H), 0.82 (t, J=8.1Hz, 2H), −0.06 (s, 9H)

Example 18N-(4-formylbenzyl)-N-(1H-imidazol-2-ylmethyl)-1H-imidazole-2-carboxamide(Compound 18)

To the compound 17 (970 mg), a 50% trifluoroacetic acid-dichloromethanesolution (10 mL) was added under ice cooling. The reaction mixture wasstirred at room temperature for 3 hours. The reaction mixture wasconcentrated and an aqueous 2N sodium hydroxide solution (20 mL) wasadded to the residue. The aqueous layer was extracted twice withdichloromethane (50 mL). The combined organic layer was washed withsaturated brine, dried over anhydrous magnesium sulfate and thenconcentrated to obtain the title compound having the following physicalproperties. The obtained compound was used in the following reactionwithout being purified.

TLC: Rf 0.50 (chloroform:methanol: 28% ammonia water=80:10:1);

NMR (CDCl₃): δ 9.98 (s, 1H), 7.81 (d, J=8.1 Hz, 2H), 7.49 (d, J=8.1 Hz,2H), 7.23 (s, 2H), 7.08 (s, 2H), 5.04 (s, 2H), 4.87 (s, 2H)

Example 19N-[4-({[4-(dipropylamino)butyl]amino}methyl)benzyl]-N-(1H-imidazol-2-ylmethyl)-1H-imidazole-2-carboxamide(Compound 19)

The same procedure as a series of reactions of Example 1 was carriedout, except that the compound 18 was used in place of1-({[2-(trimethylsilyl)ethyloxy]methyl}-1H-imidazole-2-carboaldehyde andN,N-dipropyl-1,4-butanediamine was used in place of{[4-(methyloxy)phenyl]methyl}amine, to obtain the title compound havingthe following physical properties.

Description: semisolid;

TLC: Rf 0.64 (chloroform:methanol: 28% ammonia water=80:20:4);

NMR (DMSO-d₆): δ 0.80 (t, J=7.5 Hz, 6H), 1.22-1.51 (m, 8H), 2.16-2.35(m, 6H), 2.44 (t, J=6.3 Hz, 2H), 3.63 (s, 2H), 4.48 (s, 1H), 4.54 (s,1H), 5.25 (s, 1H), 5.53 (s, 1H), 6.72-6.92 (m, 1H), 6.92-7.10 (m, 1H),7.11-7.22 (m, 3H), 7.22-7.36 (m, 3H), 11.70-12.25 (m, 2H)

Example 19(1) to Example 19(19)

The same procedure as a series of reactions of Example 16→Example17→Example 18→Example 19 was carried out, except that1-(1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-imidazol-2-yl)methaneaminewas used or a corresponding amine compound was used in place of it inthe process of Example 16, 1H-imidazole-2-carboxylic acid was used or acorresponding carboxylic acid was used in place of it the process ofExample 17, and N,N-dipropyl-1,4-butanediamine was used or acorresponding amine was used in place of it in the process of Example19, to obtain the following compound of the present invention.

Example 19(1)N-[4-({[4-(dipropylamino)butyl]amino}methyl)benzyl]-N-[(5-methyl-2-pyridinyl)methyl]-1H-imidazole-2-carboxamide(Compound 19-1)

Description: oily product;

TLC: Rf 0.27 (chloroform:methanol: 28% ammonia water=80:10:1);

NMR (DMSO-d₆): δ 0.80 (t, J=7.5 Hz, 6H), 1.25-1.46 (m, 8H), 2.18-2.34(m, 9H), 2.37-2.47 (m, 2H), 3.62 (s, 2H), 4.53 (s, 1H), 4.57 (s, 1H),5.48 (s, 1H), 5.52 (s, 1H), 6.86-7.41 (m, 7H), 7.47-7.61 (m, 1H),8.24-8.40 (m, 1H), 12.50-13.44 (m, 1H)

Example 19(2)N-[4-({[4-(dipropylamino)butyl]amino}methyl)benzyl]-N-(1H-imidazol-2-ylmethyl)-4-methyl-1H-imidazole-2-carboxamide(Compound 19-2)

TLC: Rf 0.47 (dichloromethane:methanol: 28% ammonia water=90:10:2);

NMR (CDCl₃): δ 0.86 (m, 6H), 1.38-1.60 (m, 8H), 2.15-2.50 (m, 9H),2.58-2.68 (m, 2H), 3.78 (m, 2H), 4.45-4 98(m, 4H), 6.92-7.42 (m, 7H)

Example 19(3)N-[4-({[4-(dipropylamino)butyl]amino}methyl)benzyl]-N-(1H-imidazol-2-ylmethyl)-2-pyridinecarboxamide(Compound 19-3)

Description: oily product;

TLC: Rf 0.20 (chloroform:methanol: 28% ammonia water=80:10:1);

NMR (DMSO-d₆): δ 0.75-0.90 (m, 6H), 1.26-1.47 (m, 8H), 2.16-2.37 (m,6H), 2.38-2.47 (m, 2H), 3.63 (s, 1H), 3.66 (s, 1H), 4.47 (s, 1H), 4.52(s, 1H), 4.57 (s, 1H), 4.59 (s, 1H), 6.77-6.91 (m, 1H), 7.05 (s, 1H),7.15 (d, J=7.8 Hz, 1H), 7.19-7.35 (m, 3H), 7.42-7.55 (m, 1H), 7.59-7.76(m, 1H), 7.84-8.02 (m, 1H), 8.50-8.72 (m, 1H), 11.71-12.10 (m, 1H)

Example 19(4)N-[4-({[4-(dipropylamino)butyl]amino}methyl)benzyl]-N-(2-pyridinylmethyl)-1H-imidazole-2-carboxamide(Compound 19-4)

Description: oily product

TLC: Rf 0.31 (chloroform:methanol: 28% ammonia water=80:10:1);

NMR (DMSO-d₆): δ 0.80 (t, J=7.5 Hz, 6H), 1.24-1.48 (m, 8H), 2.17-2.36(m, 6H), 2.37-2.46 (m, 2H), 3.63 (s, 2H), 4.51-4.68 (m, 2H), 5.47-5.64(m, 2H), 6.91-7.14 (m, 1H), 7.24 (t, 7H), 7.73 (t, J=7.5 Hz, 1H),8.38-8.63 (m, 1H)

Example 19(5)N-[4-({[4-(dipropylamino)butyl]amino}methyl)benzyl]-N-(2-pyridinylmethyl)-2-pyridinecarboxamide(Compound 19-5)

Description: oily product;

TLC: Rf 0.29 (chloroform:methanol: 28% ammonia water=80:10:1);

NMR (DMSO-d₆): δ 0.70-0.89 (m, 6H), 1.24-1.48 (m, 8H), 2.16-2.36 (m,6H), 2.36-2.47 (m, 2H), 3.57-3.72 (m, 2H), 4.51-4.73 (m, 4H), 7.11-7.18(m, 1H), 7.19-7.38 (m, 5H), 7.39-7.51 (m, 1H), 7.56-7.67 (m, 1H),7.67-7.97 (m, 2H), 8.41-8.68 (m, 2H)

Example 19(6)N-[4-({[4-(dipropylamino)butyl]amino}methyl)benzyl]-N-(1H-imidazol-2-ylmethyl)-1H-pyrrole-2-carboxamide(Compound 19-6)

Description: oily product;

TLC: Rf 0.27 (chloroform:methanol: 28% ammonia water=80:10:1);

NMR (DMSO-d₆): δ 0.81 (t, J=7.50 Hz, 6H), 1.18-1.51 (m, 8H), 2.15-2.40(m, 8H), 3.72 (s, 2H), 4.31-4.48 (m, 2H), 4.51-4.72 (m, 2H), 5.97-6.14(m, 1H), 6.73-7.47 (m, 9H), 11.58-12.28 (m, 1H)

Example 19(7)N-[4-({[4-(dipropylamino)butyl]amino}methyl)benzyl]-N-(5,6,7,8-tetrahydro-8-quinolinyl)-1H-imidazole-2-carboxamide(Compound 19-7)

Description: oily product;

TLC: Rf 0.43 (chloroform:methanol: 28% ammonia water=80:10:1);

NMR (DMSO-d₆): δ 0.80 (t, J=7.2 Hz, 6H), 1.22-1.51 (m, 8H), 1.61-2.08(m, 2H), 2.17-2.35 (m, 8H), 2.36-2.47 (m, 2H), 2.58-2.96 (m, 2H),3.51-3.71 (m, 3H), 4.73-5.11 (m, 1H), 5.34-5.67 (m, 1H), 6.92-7.36 (m,7H), 7.39-7.59 (m, 1H), 8.19-8.43 (m, 1H), 12.43-13.40 (m, 1H)

Example 19(8)N-[4-({[4-(dipropylamino)butyl]amino}methyl)benzyl]-N-(1H-imidazol-2-ylmethyl)-1H-pyrazole-4-carboxamide(Compound 19-8)

Description: oily product;

TLC: Rf 0.15 (ethyl acetate: methanol: 28% ammonia water=80:20:3);

NMR (DMSO-d₆): δ 7.40-6.70 (m, 8H), 4.60-4.38 (m, 4H), 3.76 (m, 2H),2.57 (m, 2H), 2.40-2.22 (m, 6H), 1.60-1.28 (m, 8H), 0.82 (t, J=7.2 Hz,6H)

Example 19(9)N-[4-({[4-(dipropylamino)butyl]amino}methyl)benzyl]-N-(1H-imidazol-2-ylmethyl)-1H-imidazole-4-carboxamide(Compound 19-9)

Description: oily product;

TLC: Rf 0.12 (ethyl acetate: methanol: 28% ammonia water=80:20:3);

NMR (DMSO-d₆): δ 7.88-7.65 (m, 2H), 7.35-7.18 (m, 4H), 7.18-6.75 (m,2H), 5.42-4.85 (m, 2H), 4.60-4.35 (m, 2H), 3.63 (m, 2H), 2.55-2.38 (m,2H), 2.38-2.00 (m, 6H), 1.48-1.24 (m, 8H), 0.80 (t, J=7.2 Hz, 6H)

Example 19(10)N-(1H-imidazol-2-ylmethyl)-N-[4-({[4-(1-piperidinyl)butyl]amino}methyl)benzyl]-1H-imidazole-2-carboxamide(Compound 19-10)

Description: amorphous;

TLC: Rf 0.55 (chloroform:methanol: 28% ammonia water=80:20:4);

NMR (DMSO-d₆): δ 1.21-1.56 (m, 10H), 2.07-2.20 (m, 2H), 2.19-2.35 (m,4H), 2.36-2.47 (m, 2H), 3.63 (s, 2H), 4.48 (s, 1H), 4.54 (s, 1H), 5.25(s, 1H), 5.53 (s, 1H), 6.72-7.10 (m, 3H), 7.10-7.36 (m, 5H), 11.68-12.15(m, 2H)

Example 19(11)N-(1H-imidazol-2-ylmethyl)-N-[4-({[4-(4-morpholinyl)butyl]amino}methyl)benzyl]-1H-imidazole-2-carboxamide(Compound 19-11)

Description: amorphous;

TLC: Rf 0.58 (chloroform:methanol: 28% ammonia water=80:10:1);

NMR (DMSO-d₆): δ 1.34-1.48 (m, 4H), 2.14-2.25 (m, 2H), 2.24-2.36 (m,4H), 2.38-2.48 (m, 2H), 3.46-3.58 (m, 4H), 3.65 (s, 2H), 4.48 (s, 1H),4.55 (s, 1H), 5.26 (s, 1H), 5.53 (s, 1H), 6.70-6.92 (m, 1H), 6.93-7.12(m, 1H), 7.13-7.24 (m, 3H), 7.24-7.45 (m, 3H), 11.65-12.34 (m, 2H)

Example 19(12)N-(1H-benzoimidazol-2-ylmethyl)-N-[4-({[4-(dipropylamino)butyl]amino}methyl)benzyl]-1H-imidazole-2-carboxamide(Compound 19-12)

Description: amorphous;

TLC: Rf 0.26 (chloroform:methanol: 28% ammonia water=80:10:1);

NMR (DMSO-d₆): δ 0.80 (t, J=7.5 Hz, 6H), 1.24-1.50 (m, 8H), 2.19-2.38(m, 8H), 3.67 (s, 2H), 4.68 (s, 2H), 5.61 (s, 1H), 5.65 (s, 1H),6.99-7.19 (m, 3H), 7.19-7.37 (m, 5H), 7.37-7.50 (m, 1H), 7.50-7.65 (m,1H), 12.10-12.70 (m, 2H)

Example 19(13)N-[4-({[4-(3,4-dihydro-2(1H)-isoquinolinyl)butyl]amino}methyl)benzyl]-N-(1H-imidazol-2-ylmethyl)-1H-imidazole-2-carboxamide(Compound 19-13)

Description: amorphous;

TLC: Rf 0.36 (chloroform:methanol: 28% ammonia water=80:10:1);

NMR (DMSO-d₆): δ 1.34-1.66 (m, 4H), 2.39 (t, J=6.9 Hz, 2H), 2.44-2.56(m, 2H), 2.59 (t, J=6.0 Hz, 2H), 2.76 (t, J=6.0 Hz, 2H), 3.48 (s, 2H),3.64 (s, 2H), 4.48 (s, 1H), 4.54 (s, 1H), 5.25 (s, 1H), 5.53 (s, 1H),6.73-6.91 (m, 1H), 6.92-7.13 (m, 5H), 7.12-7.43 (m, 6H), 11.70-12.33 (m,2H)

Example 19(14)N-[4-({[4-(dipropylamino)butyl]amino}methyl)benzyl]-2-(1H-imidazole-4-yl)-N-(1H-imidazol-2-ylmethyl)acetamide(Compound 19-14)

Description: oily product;

TLC: Rf 0.36 (ethyl acetate: methanol: 28% ammonia water=20:2:1);

NMR (DMSO-d₆): δ 0.81 (t, J=7.5 Hz, 6H), 1.20-1.50 (m, 8H), 2.20-2.40(m, 8H), 3.60-3.95 (m, 4H), 4.38-4.68 (m, 4H), 6.78-7.35 (m, 7H), 7.60(m, 1H)

Example 19(15)N-[4-({[4-(dipropylamino)butyl]amino}methyl)benzyl]-N-(1H-imidazol-2-ylmethyl)-1H-pyrazole-3-carboxamide(Compound 19-15)

Description: oily product;

TLC: Rf 0.35 (dichloromethane:methanol: 28% ammonia water=90:10:3);

NMR (CDCl₃): δ 0.86 (t, J=7.5 Hz, 6H), 1.39-1.60 (m, 8H), 2.30-2.52 (m,6H), 2.58-2.72 (m, 2H), 3.72-3.84 (m, 2H), 4.56-5.05 (m, 4H), 6.70-6.94(m, 1H), 6.95-7.05 (m, 2H), 7.18-7.40 (m, 4H), 7.55-7.68 (m, 1H)

Example 19(16)N-[4-({[4-(dipropylamino)butyl]amino}methyl)benzyl]-N-(1H-imidazol-2-ylmethyl)-4-methyl-1H-imidazole-2-carboxamide(Compound 19-16)

Description: oily product;

TLC: Rf 0.47 (dichloromethane:methanol: 28% ammonia water=90:10:2);

NMR (CDCl₃): δ d 0.86 (m, 6H), 1.38-1.60 (m, 8H), 2.15-2.50 (m, 9H),2.58-2.68 (m, 2H), 3.78 (m, 2H), 4.45-4 98(m, 4H), 6.92-7.42 (m, 7H),8.40-8.50 (m, 1H)

Example 19(17)N-[4-({[4-(dipropylamino)butyl]amino}methyl)benzyl]-N-(1H-imidazol-2-ylmethyl)-5-methyl-2-pyridinecarboxamide(Compound 19-17)

Description: oily product;

TLC: Rf 0.16 (ethyl acetate: methanol: 28% ammonia water=90:10:2);

NMR (CDCl₃): δ d 0.87 (t, J=7.5 Hz, 6H), 1.38-1.60 (m, 8H), 2.30-2.55(m, 6H), 2.43 (s, 3H), 2.64 (t, J=6.0 Hz, 2H), 3.78 (s, 2H), 4.40-4.78(m, 4H), 7.00-7.18 (m, 2H), 7.29 (d, J=8.1 Hz, 2H), 7.39 (d, J=8.1 Hz,2H), 7.59-7.82 (m, 2H), 8.40-8.50 (m, 1H)

Example 19(18)N-[4-({[4-(dipropylamino)butyl]amino}methyl)benzyl]-N-(1H-imidazol-2-ylmethyl)-1H-1,2,4-triazole-5-carboxamide(Compound 19-18)

Description: amorphous;

TLC: Rf 0.33 (dichloromethane:methanol: 28% ammonia water=80:20:3);

NMR (CDCl₃): δ d 0.78-0.95 (m, 6H), 1.35-1.78 (m, 8H), 2.40-2.68 (m,6H), 2.70-2.85 (m, 2H), 3.80-4.00 (m, 2H), 4.52-5.12 (m, 4H), 6.95-7.12(m, 2H), 7.08-7.40 (m, 4H), 8.10-8.21 (m, 1H)

Example 19(19)N-(4-{[{4-[(dipropylamino)methyl]benzyl}(methyl)amino]methyl}benzyl)-N-(1H-imidazol-2-ylmethyl)-1H-imidazole-2-carboxamide

Description: amorphous;

TLC: Rf 0.44 (dichloromethane:methanol: 28% ammonia water=90:10:1);

NMR (CDCl₃): δ 0.84 (t, J=7.2 Hz, 6H), 1.41-1.53 (m, 4H), 2.16 (s, 3H),2.33-2.38 (m, 4H), 3.48 (s, 2H), 3.49 (s, 2H), 3.53 (s, 2H), 4.77 (s,2H), 4.97 (s, 2H), 6.94-7.38 (m, 12H)

Example 20 Methyl2-[4-(dipropylamino)butyl]-1,2,3,4-tetrahydro-6-isoquinolinecarboxylate(Compound 20)

The same procedure as a series of reactions of Example 1 was carriedout, except that 4-(dipropylamino)butanal was used in place of1-({[2-(trimethylsilyl)ethyloxy]methyl}-1H-imidazole-2-carboaldehyde andmethyl 1,2,3,4-tetrahydro-6-isoquinolinecarboxylate hydrochloride wasused in place of {[4-(methyloxy)phenyl]methyl}amine, to obtain the titlecompound having the following physical properties.

TLC: Rf 0.30 (chloroform:methanol: 28% ammonia water=80:10:1);

NMR (CDCl₃): δ 7.77 (m, 2H), 7.08 (d, J=7.8 Hz, 1H), 3.890 (s, 3H), 3.66(s, 2H), 2.94 (t, J=6.3 Hz, 2H), 2.74 (t, J=6.0 Hz, 2H), 2.47 (m, 2H),2.42 (m, 2H), 2.36 (m, 4H), 1.28 (m, 6H), 0.86 (m, 8H)

Example 21{2-[4-(dipropylamino)butyl]-1,2,3,4-tetrahydro-6-isoquinolinyl}methanol(Compound 21)

To a solution of the compound 20 (3.02 g) in tetrahydrofuran (25 mL),lithium aluminum hydride (330 mg) was added at −78° C. The reactionmixture was stirred at −78° C. for 30 minutes. To the reaction mixture,sodium sulfate decahydrate (2.8 g) and diethylether (100 mL) were addedand the temperature of the reaction mixture was raised to roomtemperature. The reaction mixture was filtered through Celite (tradename) and then concentrated. The residue was purified by silica gelcolumn chromatography (ethylacetate:methanol:triethylamine=10:1:0→10:1:0.5) to obtain the titlecompound (2.28 g) having the following physical properties.

TLC: Rf 0.57 (chloroform:methanol: 28% ammonia water=80:10:1);

NMR (CDCl₃): δ 7.10 (m, 2H), 7.00 (d, J=8.4 Hz, 1H), 4.62 (s, 2H), 3.61(s, 2H), 2.89 (t, J=5.7 Hz, 2H), 2.72 (t, J=5.7 Hz, 2H), 2.51 (m, 2H),2.45 (m, 2H), 2.37 (m, 4H), 1.46 (m, 8H), 0.83 (m, 6H)

Example 222-[4-(dipropylamino)butyl]-1,2,3,4-tetrahydro-6-isoquinolinecarboaldehyde(Compound 22)

To a solution of the compound 21 (2.28 g) and triethylamine (4.99 mL) indimethyl sulfoxide (30 mL), a sulfur trioxide-pyridine complex (2.28 g)was slowly added. The reaction mixture was stirred at room temperaturefor one hour. To the reaction solution, water (100 mL) was added. Theaqueous layer was extracted twice with dichloromethane (50 mL). Thecombined organic layer was washed with saturated brine, dried overanhydrous magnesium sulfate and then concentrated. The residue waspurified by silica gel chromatography (ethylacetate:methanol:triethylamine=10:1:0→10:1:0.5) to obtain the titlecompound (2.26 g) having the following physical properties.

TLC: Rf 0.40 (chloroform:methanol: 28% ammonia water=80:10:1);

NMR (CDCl₃): δ 9.94 (s, 1H), 7.62 (m, 2H), 7.18 (d, 8.4 Hz, 1H), 3.69(s, 2H), 2.98 (t, J=5.7 Hz, 2H), 2.75 (t, J=6.3 Hz, 2H), 2.54 (m, 2H),2.42 (m, 2H), 2.34 (m, 4H), 1.46 (m, 8H), 0.87 (m, 6H)

Example 23N-({2-[4-(dipropylamino)butyl]-1,2,3,4-tetrahydro-6-isoquinolinyl}methyl)-N-[(1-methyl-1H-imidazol-2-yl)methyl]-1H-imidazole-2-carboxamide(Compound 23)

The same procedure as a series of reactions of Example 1 was carriedout, except that the compound 22 was used in place of1-({[2-(trimethylsilyl)ethyloxy]methyl}-1H-imidazole-2-carboaldehyde and[(1-methyl-1H-imidazol-2-yl)methyl]amine was used in place of{[4-(methyloxy)phenyl]methyl}amine to obtain({2-[4-(dipropylamino)butyl]-1,2,3,4-tetrahydro-6-isoquinolinyl}methyl)[(1-methyl-1H-imidazol-2-yl)methyl]amine(compound 23-a). The same procedure as a series of reactions of Example17 was carried out, except that the obtained compound 23-a was used inplace of the compound 16, to obtain the title compound having thefollowing physical properties.

Description: oily product;

TLC: Rf 0.28 (chloroform:methanol: 28% ammonia water=80:10:1);

NMR (DMSO-d₆): δ 0.81 (t, J=7.50 Hz, 6H), 1.26-1.44 (m, 6H), 1.44-1.59(m, 2H), 2.21-2.32 (m, 4H), 2.32-2.37 (m, 2H), 2.37-2.46 (m, 2H),2.55-2.66 (m, 2H), 2.67-2.83 (m, 2H), 3.43-3.53 (m, 3H), 3.57 (s, 1H),3.62 (s, 1H), 4.34-4.51 (m, 2H), 4.51-4.66 (m, 1H), 5.46-5.62 (m, 1H),6.75-6.80 (m, 1H), 6.80-6.84 (m, 1H), 6.87-7.01 (m, 2H), 7.01-7.15 (m,2H), 7.28 (s, 1H), 13.04 (s, 1H)

Example 23(1) to Example 23(2)

The same procedure as a series of reactions of Example 17 was carriedout, except that the compound 23-a was used in place of the compound 16and 2-pyridinecarboxylic acid or 3-pyridinecarboxylic acid was used inplace of 1H-imidazole-2-carboxylic acid, to obtain the title compoundhaving the following physical properties.

Example 23(1)N-({2-[4-(dipropylamino)butyl]-1,2,3,4-tetrahydro-6-isoquinolinyl}methyl)-N-[(1-methyl-1H-imidazol-2-yl)methyl]-2-pyridinecarboxamide(Compound 23-1)

Description: oily product;

TLC: Rf 0.60 (chloroform:methanol: 28% ammonia water=80:20:4);

NMR (DMSO-d₆): δ 0.82 (t, J=7.2 Hz, 6H), 1.22-1.44 (m, 6H), 1.44-1.61(m, 2H), 2.19-2.45 (m, 8H), 2.54-2.66 (m, 2H), 2.67-2.82 (m, 2H),3.40-3.56 (m, 2H), 3.64 (s, 2H), 4.50 (s, 2H), 4.54-4.71 (m, 3H),6.70-7.07 (m, 4H), 7.12 (s, 1H), 7.39-7.52 (m, 1H), 7.58 (t, J=7.5 Hz,1H), 7.89 (td, J=7.5, 1.8 Hz, 1H), 8.56 (t, J=4.8 Hz, 1H)

Example 23(2)N-({2-[4-(dipropylamino)butyl]-1,2,3,4-tetrahydro-6-isoquinolinyl}methyl)-N-[(1-methyl-1H-imidazol-2-yl)methyl]nicotinamide(Compound 23-2)

Description: oily product;

TLC: Rf 0.60 (chloroform:methanol: 28% ammonia water=80:20:4);

NMR (DMSO-d₆): δ 0.82 (t, J=7.2 Hz, 6H), 1.25-1.45 (m, 6H), 1.45-1.61(m, 2H), 2.18-2.46 (m, 8H), 2.54-2.66 (m, 2H), 2.67-2.86 (m, 2H), 3.48(s, 2H), 3.64 (s, 2H), 4.27-4.38 (m, 1H), 4.39-4.45 (m, 1H), 4.45-4.51(m, 1H), 4.51-4.59 (m, 1H), 4.59-4.72 (m, 1H), 6.69-7.22 (m, 5H), 7.42(dd, J=7.8, 4.8 Hz, 1H), 7.71-8.06 (m, 1H), 8.52-8.82 (m, 2H)

Example 24 Methyl 4-[(5-hydroxypentyl)oxy]benzoate (Compound 24)

To a solution of methyl p-hydroxybenzoate (3.38 g), 1,5-pentanediol(2.30 g) and triphenylphosphine (5.96 g) in tetrahydrofuran (100 mL),diethylazodicarboxylate (2.2N toluene solution, 10 mL) was slowly addedunder ice cooling. The reaction mixture was stirred under ice coolingfor 16 hours. The reaction mixture was concentrated and the residue waswashed with n-hexane-ethyl acetate solution (n-hexane:ethylacetate=1:1). The reaction mixture was concentrated and then purified bysilica gel chromatography (n-hexane:ethyl acetate=64:36→45:55) to obtainthe title compound (3.58 g) having the following physical properties.

TLC: Rf 0.36 (n-hexane:ethyl acetate=1:1);

NMR (CDCl₃): δ 1.47-1.73 (m, 4H), 1.76-1.92 (m, 2H), 3.69 (t, J=6.3 Hz,2H), 3.88 (s, 3H), 4.02 (t, J=6.3 Hz, 2H), 6.90 (d, J=9.0 Hz, 2H), 7.97(d, J=9.0 Hz, 2H)

Example 25 Methyl 4-({5-[(methylsulfonyl)oxy]pentyl}oxy)benzoate(Compound 25)

To a solution of the compound 24 (3.58 g) and triethylamine (2.76 mL) inethyl acetate (50 mL), methanesulfonyl chloride (0.78 mL) was slowlyadded under ice cooling. The reaction mixture was stirred under icecooling for 2 hours. The reaction mixture was diluted with ethyl acetate(100 mL). The diluted solution was washed in turn with water andsaturated brine, dried over anhydrous magnesium sulfate and thenconcentrated. The residue was purified by silica gel chromatography(ethyl acetate:methanol=1:0→10:1) to obtain the title compound (3.70 g)having the following physical properties.

TLC: Rf 0.39 (n-hexane:ethyl acetate=1:1);

NMR (CDCl₃): δ 1.56-1.69 (m, 2H), 1.76-1.93 (m, 4H), 3.01 (s, 3H), 3.89(s, 3H), 4.03 (t, J=6.3 Hz, 2H), 4.27 (t, J=6.3 Hz, 2H), 6.90 (d, J=9.0Hz, 2H), 7.98 (d, J=9.0 Hz, 2H)

Example 26 Methyl 4-{[5-(dipropylamino)pentyl]oxy}benzoate (Compound 26)

A solution of the compound 25 (3.70 g) and dipropylamine (3.00 mL) inisopropyl alcohol (40 mL) was stirred at 60° C. for 16 hours. Thereaction mixture was cooled to room temperature and saturated sodiumbicarbonate water (40 mL) was added. The aqueous layer was extractedtwice with ethyl acetate (50 mL). The combined organic layer was washedwith saturated brine, dried over anhydrous magnesium sulfate and thenconcentrated. The residue was purified by silica gel chromatography(ethyl acetate:methanol=10:90→0:100) to obtain the title compound (1.94g) having the following physical properties.

TLC: Rf 0.30 (ethyl acetate:methanol=10:90);

NMR (CDCl₃): δ 0.87 (t, J=7.5 Hz, 6H), 1.33-1.59 (m, 8H), 1.73-1.91 (m,2H), 2.29-2.39 (m, 4H), 2.39-2.48 (m, 2H), 3.88 (s, 3H), 4.01 (t, J=6.6Hz, 2H), 6.89 (d, J=9.0 Hz, 2H), 7.97 (d, J=9.0 Hz, 2H)

Example 274-{[5-(dipropylamino)pentyl]oxy}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 27)

The same procedure as a series of reactions of Example 5→Example6→Example 10 was carried out, except that the compound 26 was used inplace of the compound 4 in the process of Example 5, to obtain the titlecompound having the following physical properties.

Description: amorphous;

TLC: Rf 0.66 (dichloromethane:methanol: 28% ammonia water=16:4:1);

NMR (CDCl₃): δ 0.86 (t, J=7.5 Hz, 6H), 1.31-1.57 (m, 8H), 1.69-1.87 (m,2H), 2.28-2.49 (m, 6H), 3.96 (t, J=6.6 Hz, 2H), 4.65 (s, 4H), 6.89 (d,J=8.7 Hz, 2H), 6.93-7.12 (m, 4H), 7.66 (d, J=8.7 Hz, 2H)

Example 28 (5E)-6-[4-(methoxycarbonyl)phenyl]-5-hexanoic acid (Compound28)

A solution of 5-(triphenylphosphonium)pentanoic acid bromide (26.8 g) intoluene (200 mL), t-butoxy potassium (13.5 g) was added. The reactionmixture was stirred at 80° C. for one hour. The reaction mixture wascooled to 0° C. and 4-methyl formylbenzoate (3.41 g) was added to thereaction mixture. The reaction mixture was stirred at 0° C. for 2 hoursand then stirred at room temperature for 30 minutes. To the reactionmixture, water (150 mL) was added. The aqueous layer was acidified byadding 5N hydrochloric acid (15 mL) and the aqueous layer was extractedtwice with ethyl acetate (100 mL). The combined organic layer was washedwith saturated brine, dried over anhydrous magnesium sulfate and thenconcentrated. The residue was purified by silica gel chromatography(n-hexane:ethyl acetate=3:1→2:1) to obtain the title compound (3.10 g)having the following physical properties.

TLC: Rf 0.50 (n-hexane:ethyl acetate=1:9);

NMR (CDCl₃): δ 1.71-1.94 (m, 2H), 2.23-2.50 (m, 4H), 3.84-3.96 (m, 3H),5.66-6.39 (m, 1H), 6.40-6.56 (m, 1H), 7.28-7.44 (m, 2H), 7.90-8.06 (m,2H)

Example 29 Methyl 4-[(1E)-6-hydroxy-1-hexene-1-yl]benzoate (Compound 29)

To a solution of the compound 28 (3.10 g) in tetrahydrofuran (40 mL), aborane-tetrahydrofuran complex (0.98 N/THF, 13 mL) was slowly addedunder ice cooling. The reaction mixture was stirred at 0° C. for 20minutes. To the reaction mixture, water (10 mL) was slowly added andfurthermore potassium carbonate (3 g) was added. This solution wasdiluted with water (100 mL) and the aqueous layer was extracted twicewith ethyl acetate (100 mL). The combined organic layer was washed withsaturated brine, dried over anhydrous magnesium sulfate and thenconcentrated. The residue was purified by silica gel chromatography(n-hexane:ethyl acetate=7:3→3:7) to obtain the title compound (2.50 g)having the following physical properties.

TLC: Rf 0.45 (n-hexane:ethyl acetate=1:1);

NMR (CDCl₃): δ 1.47-1.74 (m, 4H), 2.21-2.72 (m, 2H), 3.55-3.76 (m, 2H),3.82-3.97 (m, 3H), 5.68-6.54 (m, 2H), 7.28-7.44 (m, 2H), 7.88-8.06 (m,2H)

Example 30 Methyl 4-{6-[(methylsulfonyl)oxy]hexyl}benzoate (Compound 30)

In a solution of the compound 29 (2.50 g) in methanol (30 mL), 10%palladium carbon (250 mg) was added under an argon atmosphere. Thereaction mixture was stirred under a hydrogen atmosphere at roomtemperature for 3 hours. The reaction mixture was filtered with Celite(trade name) and the filtrate was concentrated. The residue was purifiedby silica gel chromatography (n-hexane:ethyl acetate=7:3→3:7) to obtainmethyl 4-(6-hydroxyhexyl)benzoate (2.22 g). The same procedure as aseries of reactions of Example 25 was carried out, except that methyl4-(6-hydroxyhexyl)benzoate was used in place of the compound 24, toobtain the title compound having the following physical properties.

TLC: Rf 0.56 (n-hexane:ethyl acetate=1:1);

NMR (CDCl₃): δ1.29-1.52 (m, 4H), 1.58-1.83 (m, 4H), 2.59-2.73 (m, 2H),2.99 (s, 3H), 3.90 (s, 3H), 4.21 (t, J=6.6 Hz, 2H), 7.24 (d, J=8.4 Hz,2H), 7.95 (d, J=8.4 Hz, 2H)

Example 314-[6-(dipropylamino)hexyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 31)

The same procedure as a series of reactions of Example 26 was carriedout, except that the compound 30 was used in place of the compound 25,to obtain methyl 4-[6-(dipropylamino)hexyl]benzoate. The same procedureas a series of reactions of Example 5→Example 6→Example 10 was carriedout, except that methyl 4-[6-(dipropylamino)hexyl]benzoate was used inplace of the compound 4 in the process of Example 5, to obtain the titlecompound having the following physical properties.

Description: oily product;

TLC: Rf 0.37 (dichloromethane:methanol: 28% ammonia water=90:10:1);

NMR (CDCl₃): δ 0.87 (t, J=7.5 Hz, 6H), 1.14-1.71 (m, 12H), 2.30-2.49 (m,6H), 2.60 (t, J=7.5 Hz, 2H), 4.68 (m, 4H), 6.90-7.09 (m, 4H), 7.19 (d,J=8.1 Hz, 2H), 7.51 (d, J=8.1 Hz, 2H)

Example 32 Methyl 4-[(5-bromopentyl)thio]benzoate (Compound 32)

To a solution of methyl 4-mercaptobenzoate (3.39 g) and1,5-dibromopentane (9.31 g) in dimethyl formamide (100 mL), potassiumcarbonate (3.07 g) was added. The reaction mixture was stirred at roomtemperature for 3 hours. To the reaction mixture, water (300 mL) wasadded and the aqueous layer was extracted twice with an n-hexane-ethylacetate solution (n-hexane:ethyl acetate=1:1) (150 mL). The combinedorganic layer was washed with saturated brine, dried over anhydrousmagnesium sulfate and then concentrated. The residue was purified bysilica gel chromatography (n-hexane:ethyl acetate=1:0→8:2) to obtain thetitle compound (4.69 g) having the following physical properties.

TLC: Rf 0.68 (n-hexane:ethyl acetate=4:1);

NMR (CDCl₃): δ 1.54-1.80 (m, 4H), 1.80-1.98 (m, 2H), 3.00 (t, J=6.9 Hz,2H), 3.41 (t, J=6.6 Hz, 2H), 3.90 (s, 3H), 7.28 (d, J=8.7 Hz, 2H), 7.92(d, J=8.7 Hz, 2H)

Example 334-{[5-(dipropylamino)pentyl]sulfanyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 33)

The same procedure as a series of reactions of Example 26 was carriedout, except that the compound 32 was used in place of the compound 25,to obtain methyl 4-{[5-(dipropylamino)pentyl]thio}benzoate. The sameprocedure as a series of reactions of Example 5→Example 6→Example 10 wascarried out, except that methyl4-{[5-(dipropylamino)pentyl]thio}benzoate was used in place of thecompound 4 in the process of Example 5, to obtain the title compoundhaving the following physical properties.

Description: amorphous;

TLC: Rf 0.24 (dichloromethane:methanol: 28% ammonia water=80:20:1);

NMR (DMSO-d₆): δ 0.81 (t, J=7.5 Hz, 6H), 1.25-1.46 (m, 8H), 1.49-1.67(m, 2H), 2.19-2.45 (m, 6H), 2.97 (t, J=7.2 Hz, 2H), 4.56 (s, 4H),6.79-7.13 (m, 4H), 7.27 (d, J=8.4 Hz, 2H), 7.44 (d, J=8.4 Hz, 2H),10.97-13.23 (m, 2H)

Example 34 Methyl 4-{[(4-hydroxybutyl)amino]carbonyl}benzoate (Compound34)

The same procedure as a series of reactions of Example 17 was carriedout, except that 4-amino-1-butanol was used in place of the compound 16and 4-(methoxycarbonyl)benzoic acid was used in place of1H-imidazole-2-carboxylic acid, to obtain the title compound having thefollowing physical properties.

TLC: Rf 0.31 (ethyl acetate)

Example 35N¹-[4-(dipropylamino)butyl]-N⁴,N⁴-bis(1H-imidazol-2-ylmethyl)terephthalamide(Compound 35)

The same procedure as a series of reactions of Example 25→Example 26 wascarried out, except that the compound 34 was used in place of thecompound 24 in the process of Example 25, to obtain methyl4-({[4-(dipropylamino)butyl]amino}carbonyl)benzoate. The same procedureas a series of reactions of Example 5→Example 6→Example 10 was carriedout, except that methyl4-({[4-(dipropylamino)butyl]amino}carbonyl)benzoate was used in place ofthe compound 4 in the process of Example 5, to obtain the title compoundhaving the following physical properties.

Description: amorphous;

TLC: Rf 0.58 (dichloromethane:methanol: 28% ammonia water=16:4:1);

NMR (CDCl₃): δ 0.86 (t, J=7.5 Hz, 6H), 1.34-1.54 (m, 4H), 1.54-1.74 (m,4H), 2.33-2.48 (m, 4H), 2.51 (t, J=6.6 Hz, 2H), 3.31-3.56 (m, 2H), 4.55(s, 2H), 4.66 (s, 2H), 6.85-7.03 (m, 2H), 7.08 (s, 2H), 7.18-7.37 (m,1H), 7.43-7.79 (m, 4H)

Example 35(1) 4-({[4-(dipropylamino)butyl]amino}methyl)-N- (Compound35-1)

The same operation as a series of reactions of Example 35 was carriedout, except that 1-(1H-imidazol-2-yl)-N-(pyridin-3-ylmethyl)methaneaminewas used in place of the compound 2 in the process of Example 6, toobtain the title compound having the following physical properties.

Description: oily product;

TLC: Rf 0.83 (chloroform:methanol: 28% ammonia water=40:10:2);

NMR (CDCl₃): δ 0.85 (t, J=7.2 Hz, 6H), 1.22-1.70 (m, 8H), 2.24-2.48 (m,6H), 2.54-2.66 (m, 2H), 3.49 (s, 2H), 3.79 (s, 2H), 4.63 (s, 2H),6.82-7.11 (m, 2H), 7.19-7.44 (m, 5H), 7.45-7.64 (m, 1H), 8.46 (s, 1H),8.55 (d, J=1.5 Hz, 1H)

Example 36 1H-imidazole-2-sulfonyl chloride hydrochloride (Compound 36)

Under ice cooling, a chlorine gas was bubbled into a solution of2-mercaptoimidazole (3.0 g) in 1N hydrochloric acid (30 mL) for onehour. The reaction mixture was stirred under ice cooling for 2 hours.The reaction mixture was filtered and the solid collected afterfiltration was washed with ice water and then dried to obtain the titlecompound (1.76 g) having the following physical properties.

NMR (DMSO-d₆): δ 14.82 (m, 2H), 7.58 (s, 2H)

Example 37N-({2-[4-(dipropylamino)butyl]-1,2,3,4-tetrahydro-6-isoquinolinyl}methyl)-N-[(1-methyl-1H-imidazol-2-yl)methyl]-1H-imidazole-2-sulfonamide(Compound 37)

In a solution of the compound 23-a (100 mg) and pyridine (59 mL) indichloromethane (3 mL), the compound 36 (74 mg) was added. The reactionmixture was stirred at room temperature for 30 minutes. To the reactionmixture, an aqueous 1N sodium hydroxide solution (15 mL) was added. Theaqueous layer was extracted twice with dichloromethane (30 mL). Thecombined organic layer was washed with saturated brine, dried overanhydrous magnesium sulfate and then concentrated. The residue waspurified by silica gel chromatography (ethylacetate:methanol:triethylamine=10:1:0→10:1:0.5) to obtain the titlecompound (119 mg) having the following physical properties.

Description: semisolid;

TLC: Rf 0.71 (chloroform:methanol: 28% ammonia water=80:20:4);

NMR (DMSO-d₆): δ 0.82 (t, J=7.5 Hz, 6H), 1.27-1.44 (m, 6H), 1.45-1.57(m, 2H), 2.22-2.32 (m, 4H), 2.32-2.45 (m, 4H), 2.53-2.62 (m, 2H),2.62-2.70 (m, 2H), 3.41-3.49 (m, 5H), 4.26 (s, 2H), 4.41 (s, 2H), 6.59(s, 1H), 6.79 (s, 1H), 6.80-6.86 (m, 1H), 6.87-6.94 (m, 1H), 7.00 (s,1H), 7.25 (s, 2H)

Example 37(1)N-[4-({[4-(dipropylamino)butyl]amino}methyl)benzyl]-N-(1H-imidazol-2-ylmethyl)-1H-imidazole-2-sulfonamide(Compound 37-1)

The same procedure as a series of reactions of Example 20→Example21→Example 22→Example 23 was carried out, except that4-(aminomethyl)benzoic acid was used in place of methyl1,2,3,4-tetrahydro-6-isoquinolinecarboxylate hydrochloride. The sameprocedure as a series of reactions of Example 35 was carried out, exceptthat the obtainedN′-(4-{[(cyclopenta-1,3-dien-1-ylmethyl)amino]methyl}benzyl)-N,N-dipropylpropane-1,3-diaminewas used in place of the compound 23-a, to obtain the title compoundhaving the following physical properties.

Description: amorphous;

TLC: Rf 0.26 (ethyl acetate:methanol: 28% ammonia water=90:10:2);

NMR (CDCl₃): δ d 0.88 (t, J=7.2 Hz, 6H), 1.38-1.68 (m, 8H), 2.30-2.60(m, 6H), 2.74 (m, 2H), 3.70 (m, 2H), 3.77 (s, 2H), 4.56 (m, 2H),6.98-7.30 (m, 8H)

Example 384-({[4-(dipropylamino)butyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzenesulfonamide(Compound 38)

The same procedure as a series of reactions of Example 37 was carriedout, except that the compound 2 was used in place of the compound 23-aand 4-formylbenzenesulfonyl chloride was used in place of the compound36, to obtain a4-formyl-N,N-bis[(1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-imidazol-2-yl)methyl]benzamide(compound 38-a). The same procedure as a series of reactions of Example1→Example 10 was carried out, except that the compound 38-a was used inplace of1-({[2-(trimethylsilyl)ethyloxy]methyl}-1H-imidazole-2-carboaldehyde andN,N-dipropyl-1,4-butanediamine was used in place of{[4-(methyloxy)phenyl]methyl}amine in the process of Example 1, toobtain the title compound having the following physical properties.

Description: amorphous;

TLC: Rf 0.66 (chloroform:methanol: 28% ammonia water=80:20:4);

NMR (DMSO-d₆): δ 0.81 (t, J=7.5 Hz, 6H), 1.24-1.48 (m, 8H), 2.18-2.37(m, 6H), 2.42 (t, J=6.3 Hz, 2H), 3.70 (s, 2H), 4.49 (s, 4H), 6.68-6.91(m, 2H), 6.91-7.16 (m, 2H), 7.40 (d, J=8.1 Hz, 2H), 7.54 (d, J=8.1 Hz,2H), 12.19-12.64 (m, 2H)

Example 39N-[4-({[4-(dimethylamino)butyl]amino}carbonyl)benzyl]-N-(1H-imidazol-2-ylmethyl)-1H-imidazole-2-carboxamide(Compound 39)

The same procedure as a series of reactions of Example 16→Example 17 wascarried out, except that methyl 4-formylbenzoate was used in place of4-(diethoxymethyl)benzaldehyde, to obtain methyl4-({(1H-imidazol-2-ylcarbonyl)[(1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-imidazol-2-yl)methyl]amino}methyl)benzoate(compound 39-a). The same procedure as a series of reactions of Example5→Example 6→Example 10 was carried out, except that the compound 39-awas used in place of the compound 4 in the process of Example 5 andN,N-dimethyl-1,4-butaneamine was used in place of the compound 2 in theprocess of Example 6, to obtain the title compound having the followingphysical properties.

Description: amorphous;

TLC: Rf 0.24 (chloroform:methanol: 28% ammonia water=80:20:4);

NMR (DMSO-d₆): δ 1.32-1.64 (m, 4H), 2.09 (s, 6H), 2.13-2.27 (m, 2H),3.14-3.29 (m, 2H), 3.65 (s, 1H), 3.71 (s, 1H), 4.52 (s, 0.5H), 4.62 (s,0.5H), 5.31 (s, 0.5H), 5.60 (s, 0.5H), 6.71-6.89 (m, 1H), 6.94-7.11 (m,1H), 7.24-7.35 (m, 1H), 7.41 (d, J=8.1 Hz, 1H), 7.77 (d, J=8.1 Hz, 2H),7.92-8.08 (m, 2H), 8.35-8.50 (m, 1H), 8.62-8.78 (m, 1H), 10.06 (s, 1H)

Example 39(1)N-[4-({[4-(dipropylamino)butyl]amino}carbonyl)benzyl]-N-(1H-imidazol-2-ylmethyl)-1H-imidazole-2-carboxamide(Compound 39-1)

The same procedure as a series of reactions of Example 39 was carriedout, except that N,N-dipropyl-1,4-butaneamine was used in place ofN,N-dimethyl-1,4-butaneamine, to obtain the title compound having thefollowing physical properties.

Description: amorphous;

TLC: Rf 0.32 (chloroform:methanol: 28% ammonia water=80:20:4);

NMR (DMSO-d₆): δ 0.80 (t, J=7.5 Hz, 6H), 1.20-1.44 (m, 6H), 1.44-1.65(m, 2H), 2.14-2.42 (m, 6H), 3.14-3.28 (m, 2H), 4.51 (s, 1H), 4.62 (s,1H), 5.30 (s, 1H), 5.60 (s, 1H), 6.82 (s, 1H), 6.96-7.19 (m, 2H),7.22-7.44 (m, 3H), 7.77 (d, J=8.4 Hz, 2H), 8.27-8.55 (m, 1H),11.77-12.18 (m, 1H), 12.90-13.27 (m, 1H)

Example 404-formyl-N,N-bis[(1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-imidazol-2-yl)methyl]benzamide(Compound 40)

The same procedure as a series of reactions of Example 17 was carriedout, except that the compound 2 was used in place of the compound 16 and4-formylbenzoic acid was used in place of 1H-imidazole-2-carboxylicacid, to obtain the title compound having the following physicalproperties.

TLC: Rf 0.47 (chloroform:methanol=10:1);

NMR (CDCl₃): δ 10.01 (s, 1H), 7.85 (m, 2H), 7.77 (m, 2H), 7.04 (m, 4H),5.51 (s, 2H), 5.15 (s, 2H), 4.93 (s, 2H), 4.67 (s, 2H), 3.59 (t, J=8.1Hz, 2H), 3.30 (t, J=8.1 Hz, 2H), 0.94 (t, J=8.1 Hz, 2H), 0.70 (t, J=8.1Hz, 2H), 0.00 (s, 9H), −0.09 (s, 9H)

Example 41 4-formyl-N,N-bis(1H-imidazol-2-ylmethyl)benzamide (Example41)

To the compound 40 (1.7 g), a 50% trifluoroacetic acid-dichloromethanesolution (10 mL) was added. The reaction mixture was stirred at roomtemperature for 3 hours. The reaction mixture was concentrated and anaqueous 5N sodium hydroxide solution (15 mL) was added to the residue.The aqueous layer was extracted three times with 50 mL ofdichloromethane. The combined organic layer was washed with saturatedbrine and then dried over anhydrous magnesium sulfate. The solvent wasconcentrated to obtain the title compound (compound B) (922 mg) havingthe following physical properties.

TLC: Rf 0.60 (chloroform:methanol: 28% ammonia water=80:10:1);

NMR (CDCl₃): δ 10.03 (s, 1H), 7.92 (d, J=8.1 Hz, 2H), 7.74 (d, J=8.1 Hz,2H), 7.12-6.94 (m, 4H), 4.73 (s, 2H), 4.58 (s, 2H)

Example 42 Cis-N,N-dipropylcyclohexane-1,4-diamine (Compound 42)

The same procedure as a series of reactions of Example 8 was carriedout, except that tert-butyl 4-aminocyclohexylcarbamate was used in placeof the compound 7 in the process of Example 8, and a 10% hydrogenchloride-methanol solution (10 mL) was added to the obtained compound.The reaction mixture was stirred at room temperature for 2 hours. Thesolvent was concentrated and the residue was dissolved in methanol (30mL). To this solution, a carbonate resin (trade name: MP-Carbonate,manufactured by Argonaut Technologies Inc., product number: 800267, 3 g)was added, followed by stirring at room temperature for 2 hours. Thereaction mixture was filtered and then the filtrate was concentrated.The title compound (467 mg) having the following physical properties wasobtained without purifying the residue.

TLC: Rf 0.26 (dichloromethane: methanol: 28% ammonia water=80:20:3);

NMR (CDCl₃): δ 3.35-3.29 (m, 1H), 2.85-2.73 (m, 1H), 2.72-2.64 (m, 4H),1.95-1.56 (m, 12H), 0.92 (t, J=7.2 Hz, 6H)

Example 434-({[cis-4-(dipropylamino)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 43)

To a solution of the compound 41 (116 mg) and the compound 42 (203 mg)in a 1% acetic acid-dichloromethane solution (5 mL), an anhydrous sodiumsulfate (220 mg) was added. The mixture was stirred at room temperaturefor 30 minutes. To the reaction mixture, sodium triacetoxyborohydride(225 mg) was added. The mixture was stirred at room temperature for 6hours. To the reaction mixture, an aqueous saturated sodium hydrogencarbonate solution (10 mL) was added. The aqueous layer was extractedtwice with a mixture of dichloromethane: methanol=3:1 (50 mL). Thecombined organic layer was washed with saturated brine and then driedover anhydrous magnesium sulfate. The solvent was concentrated and thenpurified by silica gel chromatography to obtain the title compound (149mg) having the following physical properties.

Description: amorphous;

TLC: Rf 0.64 (dichloromethane: methanol: 28% ammonia water=80:20:3);

NMR (CDCl₃): δ 0.86 (t, J=7.2 Hz, 6H), 1.30-1.69 (m, 10H), 1.70-1.86 (m,2H), 2.34-2.57 (m, 5H), 2.71-2.83 (m, 1H), 3.76 (s, 2H), 4.53-4.81 (m,4H), 6.91-7.14 (m, 4H), 7.38 (d, J=8.1 Hz, 2H), 7.55 (d, J=8.1 Hz, 2H)

Example 43(1) to Example 43(11)

The same procedure as a series of reactions of Example 42→Example 43,except that a corresponding aldehyde or ketone was used in place oftert-butyl 4-aminocyclohexylcarbamate and a corresponding carbonylcompound was used in place of 1-propanal in the process of conductingthe same procedure as a series of reactions of Example 8 of Example 42,to obtain the title compound having the following physical properties

Example 43(1)N,N-bis(1H-imidazol-2-ylmethyl)-4-[(2-propyl-2,7-diazaspiro[3.5]non-7-yl)methyl]benzamide(Compound 43-1)

Description: amorphous;

TLC: Rf 0.23 (dichloromethane:methanol: 28% ammonia water=80:20:1);

NMR (CDCl₃): δ 0.87 (t, J=7.5 Hz, 3H), 1.23-1.44 (m, 2H), 1.72 (t,J=5.40 Hz, 4H), 2.19-2.45 (m, 6H), 2.95 (s, 4H), 3.44 (s, 2H), 4.52-4.77(m, 4H), 6.89-7.17 (m, 4H), 7.35 (d, J=8.1 Hz, 2H), 7.59 (d, J=8.1 Hz,2H)

Example 43(2)4-{[4-(dipropylamino)-1-piperidinyl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 43-2)

Description: amorphous;

TLC: Rf 0.49 (dichloromethane:methanol: 28% ammonia water=80:20:1);

NMR (CDCl₃): δ 0.85 (t, J=7.2 Hz, 6H), 1.29-1.76 (m, 8H), 1.84-2.05 (m,2H), 2.33-2.54 (m, 5H), 2.80-2.97 (m, 2H), 3.49 (s, 2H), 4.52-4.79 (m,4H), 6.91-7.15 (m, 4H), 7.36 (d, J=7.8 Hz, 2H), 7.61 (d, J=7.8 Hz, 2H)

Example 43(3)4-({[trans-4-(dipropylamino)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 43-3)

Description: amorphous;

TLC: Rf 0.57 (dichloromethane:methanol: 28% ammonia water=80:20:3);

NMR (CDCl₃): δ 0.85 (t, J=7.2 Hz, 6H), 0.98-1.33 (m, 4H), 1.33-1.51 (m,4H), 1.71-1.86 (m, 2H), 1.91-2.07 (m, 2H), 2.28-2.56 (m, 6H), 3.81 (s,2H), 4.53-4.77 (m, 4H), 6.91-7.13 (m, 4H), 7.34 (d, J=8.1 Hz, 2H), 7.54(d, J=8.1 Hz, 2H)

Example 43(4)4-[({trans-4-[cyclohexyl(propyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 43-4)

Description: amorphous;

TLC: Rf 0.60 (dichloromethane: methanol: 28% ammonia water=80:20:3);

NMR (CDCl₃): δ 0.81 (t, J=7.2 Hz, 3H), 0.96-1.46 (m, 11H), 1.51-1.65 (m,1H), 1.65-1.82 (m, 6H), 1.88-2.04 (m, 2H), 2.28-2.68 (m, 5H), 3.81 (s,2H), 4.51-4.80 (m, 4H), 6.89-7.16 (m, 4H), 7.34 (d, J=8.1 Hz, 2H), 7.55(d, J=8.1 Hz, 2H)

Example 43(5)4-[(7-cyclohexyl-2,7-diazaspiro[3.5]non-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 43-5)

Description: amorphous;

TLC: Rf 0.37 (dichloromethane:methanol: 28% ammonia water=80:20:3);

NMR (CDCl₃): δ 0.98-1.33 (m, 5H), 1.52-1.96 (m, 9H), 2.20-2.62 (m, 5H),2.99 (s, 4H), 3.63 (s, 2H), 4.52-4.81 (m, 4H), 6.92-7.14 (m, 4H), 7.29(d, J=8.1 Hz, 2H), 7.48 (d, J=8.1 Hz, 2H)

Example 43(6)4-({[3-(dipropylamino)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 43-6)

Description: amorphous;

TLC: Rf 0.32 (chloroform:methanol: 28% ammonia water=80:10:1);

NMR (DMSO-D₆): δ d 0.78 (t, J=7.5 Hz, 6H), 1.28 (m, 7H), 1.61 (m, 4H),1.94 (m, 1H), 2.28 (m, 4H), 2.86 (m, 2H), 3.65 (m, 2H), 4.58 (m, 4H),6.88 (m, 2H), 7.08 (m, 2H), 7.33 (d, J=8.1 Hz, 2H), 7.43 (d, J=8.10 Hz,2H), 12.14 (m, 1H), 12.36 (m, 1H)

Example 43(7)4-({[3-(dipropylamino)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 43-7)

Description: amorphous;

TLC: Rf 0.26 (chloroform:methanol: 28% ammonia water=80:10:1);

NMR (DMSO-D₆): δ d 0.80 (m, 6H), 1.32 (m, 6H), 1.66 (m, 3H), 1.94 (m,2H), 2.32 (m, 6H), 2.89 (m, 1H), 3.66 (m, 1H), 3.75 (m, 1H), 4.56 (m,4H), 6.97 (m, 4H), 7.33 (m, 2H), 7.42 (m, 2H), 12.21 (m, 2H)

Example 43(8)4-{[{3-[(dipropylamino)methyl]benzyl}(methyl)amino]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 43-8)

Description: amorphous;

TLC: Rf 0.32 (dichloromethane:methanol: 28% ammonia water=90:10:1);

NMR (CDCl₃): δ 0.85 (t, J=7.5 Hz, 6H), 1.41-1.53 (m, 4H), 2.16 (s, 3H),2.33-2.38 (m, 4H), 3.51 (s, 4H), 3.55 (s, 2H), 4.64 (s, 2H), 4.68 (s,2H), 6.96-7.04 (m, 4H), 7.22-7.30 (m, 4H), 7.40 (d, J=7.8 Hz, 2H), 7.59(d, J=7.8 Hz, 2H)

Example 43(9)N,N-bis(1H-imidazol-2-ylmethyl)-4-({[4-(1-pyrrolidinyl)cyclohexyl]amino}methyl)benzamide(Compound 43-9)

Description: amorphous;

TLC: Rf 0.25 (dichloromethane: methanol: 28% ammonia water=90:10:1);

NMR (CDCl₃): δ d 1.10-2.10 (m, 12H), 2.20 (m, 1H), 2.50 (m, 1H),2.65-2.88 (m, 4H), 3.82 (s, 2H), 4.55-4.80 (m, 4H), 6.98-7.12 (m, 4H),7.32-7.42 (m, 2H), 7.52-7.64 (m, 2H)

Example 43(10)4-({[2-(dipropylamino)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 43-10)

Description: amorphous;

TLC: Rf 0.70 (chloroform:methanol: 28% ammonia water=40:10:2);

NMR (CDCl₃): δ 0.80 (t, J=7.2 Hz, 3H), 0.87 (t, J=7.2 Hz, 3H), 1.00-1.56(m, 8H), 1.55-1.87 (m, 4H), 1.98-2.56 (m, 6H), 3.65 (d, J=13.5 Hz, 1H),3.88 (d, J=13.5 Hz, 1H), 4.48-4.80 (m, 4H), 6.85-7.12 (m, 4H), 7.33 (d,J=8.1 Hz, 2H), 7.57 (d, J=8.1 Hz, 2H)

Example 43(11)4-({[5-(dipropylamino)octahydro-2-pentalenyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 43-11)

Description: amorphous;

TLC: Rf 0.35 (chloroform:methanol: 28% ammonia water=40:10:2);

NMR (CDCl₃): δ 0.87 (t, J=7.2 Hz, 6H), 1.07-1.63 (m, 8H), 1.96-2.41 (m,6H), 2.44-2.63 (m, 4H), 2.87-3.19 (m, 2H), 3.79 (s, 2H), 4.49-4.79 (m,4H), 6.86-7.15 (m, 4H), 7.36 (d, J=8.1 Hz, 2H), 7.58 (d, J=8.1 Hz, 2H)

Example 44 tert-butyl4-(2-{bis[(1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-imidazol-2-yl)methyl]amino}-2-oxoethyl)-1-piperidinecarboxylate (Compound 44)

Under an argon atmosphere at room temperature,[1-(tert-butoxycarbonyl)-4-piperidinyl]acetic acid (500 mg) and thecompound 2 (899 mg) were dissolved in anhydrous dimethyl formamide (12mL), and 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide (590 mg),1-hydroxybenztriazole (417 mg) and 4-dimethylaminopyridine (377 mg) wereadded, followed by stirring at room temperature for 16 hours. After theaddition of city water and stirring, the obtained solution was extractedthree times with ethyl acetate. The organic layer was collected and thenwashed with saturated brine. After drying over anhydrous sodium sulfate,the solvent was distilled off. The residue was purified by silica gelcolumn chromatography to obtain the title compound (216 mg) having thefollowing physical properties.

TLC: Rf 0.85 (chloroform:methanol: 28% ammonia water=90:10:1);

NMR (CDCl₃): δ 7.00-6.91 (m, 4H), 5.36 (s, 2H), 5.33 (s, 2H), 4.77 (s,2H), 4.68 (s, 2H), 4.08 (m, 2H), 3.50 (m, 2H), 2.75 (m, 2H), 2.46 (d,J=6.6 Hz, 2H), 2.12 (m, 1H), 1.76 (m, 2H), 1.65 (s, 9H), 1.14 (m, 2H),0.90 (m, 2H), 0.00 (m, 18H)

Example 45 N,N-bis(1H-imidazol-2-ylmethyl)-2-(4-piperidinyl)acetamidetrihydrochloride (Compound 45)

To the compound (216 mg) prepared in Example 44, 4N hydrogenchloride/dioxane (8 mL) was added, followed by stirring at roomtemperature for 3 hours and further stirring at 70° C. for 3 hours. Thesolvent was distilled off to obtain the title compound without purifyingthe residue.

TLC: Rf 0.11 (chloroform:methanol: 28% ammonia water=40:10:2)

Example 462-{1-[4-(dipropylamino)butyl]-4-piperidinyl}-N,N-bis(1H-imidazol-2-ylmethyl)acetamide(Compound 46)

The compound 45 and 4-(dipropylamino)butanal (55.6 mg) were added and a10% acetic acid-anhydrous dimethyl formamide solution (3 mL) was stirredunder an argon atmosphere for 5 minutes. To the reaction solution,sodium triacetoxyborohydride (103 mg) was added, followed by stirring atroom temperature for 15 hours. To the reaction solution, an aqueous 2Nsodium hydroxide solution (10 mL) was added and the aqueous layer wasextracted three times with ethyl acetate. The organic layer wascollected, washed with saturated brine, dried over anhydrous sodiumsulfate, and then the solvent was distilled off. The residue waspurified by silica gel column chromatography to obtain the titlecompound (86.4 mg) having the following physical properties.

Description: oily product;

TLC: Rf 0.78 (chloroform:methanol: 28% ammonia water=40:10:2);

NMR (CDCl₃): δ 0.86 (t, J=7.2 Hz, 6H), 1.10-1.29 (m, 2H), 1.34-1.52 (m,8H), 1.53-1.77 (m, 3H), 1.77-1.95 (m, 2H), 2.19-2.51 (m, 10H), 2.70-2.94(m, 2H), 4.66 (s, 2H), 4.75 (s, 2H), 6.90-7.06 (m, 4H)

Example 46(1) to Example 46(2)

The same procedure as a series of reactions of Example 44→Example45→Example 46 was carried out, except that a corresponding carboxylicacid was used in place of [1-(tert-butoxycarbonyl)-4-piperidinyl]aceticacid and was optionally replaced by a salt, to obtain the followingcompound of the present invention

Example 46(1)1-[4-(dipropylamino)butyl]-N,N-bis(1H-imidazol-2-ylmethyl)-4-piperidinecarboxamide tetrahydrochloride (Compound 46-1)

Description: amorphous;

TLC: Rf 0.68 (chloroform:methanol: 28% ammonia water=40:10:2);

NMR (METHANOL-D₄): δ 1.02 (t, J=7.5 Hz, 6H), 1.54-1.90 (m, 7H),1.94-2.21 (m, 5H), 2.88-3.25 (m, 12H), 3.50-3.74 (m, 1H), 4.81-5.01 (m,2H), 5.42 (s, 2H), 7.50 (s, 2H), 7.61 (s, 2H)

Example 46(2)3-{1-[4-(dipropylamino)butyl]-4-piperidinyl}-N,N-bis(1H-imidazol-2-ylmethyl)propaneamide(Compound 46-2)

Description: oily product;

TLC: Rf 0.78 (chloroform:methanol: 28% ammonia water=40:10:2);

NMR (CDCl₃): δ 0.86 (t, J=7.5 Hz, 6H), 1.09-1.28 (m, 2H), 1.32-1.62 (m,11H), 1.73-1.84 (m, 2H), 2.17-2.48 (m, 12H), 2.80-2.89 (m, 2H), 4.65 (s,2H), 4.74 (s, 2H), 6.92-7.03 (m, 4H)

Example 47 Ethyl4-({bis[(1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-imidazol-2-yl)methyl]amino}carbonylamino)benzoate(Compound 47)

A solution of butyl 4-isocyanatebenzoate (250.4 mg) and the compound 2(500 mg) in dichloromethane (3 mL) was stirred at room temperature forone hour. The reaction solution was concentrated under reduced pressureand the residue was purified by silica gel column chromatography toobtain the title compound (731.9 mg) having the following physicalproperties.

TLC: Rf 0.24 (n-hexane: methanol=3:1);

NMR (CDCl₃): δ 7.98 (d, J=9.0 Hz, 2H), 7.58 (d, J=9.0 Hz, 2H), 7.04 (s,1H), 7.00 (s, 1H), 5.43 (s, 4H), 4.74 (s, 4H), 4.30 (t, J=6.6 Hz, 2H),3.50 (m, 4H), 1.75 (m, 2H), 1.49 (m, 2H), 0.98 (t, J=7.5 Hz, 3H), 0.88(m, 4H), −0.55 (s, 18H)

Example 484-({[bis(1H-imidazol-2-ylmethyl)amino]carbonyl}amino)-N-[4-(dipropylamino)butyl]benzamide(Compound 48)

To a solution of the compound 47 (195 mg) in methanol (2 mL), 2 mL ofconcentrated hydrochloric acid was added. The reaction solution wasstirred at 60° C. for 6 hours. After cooling to room temperature, thereaction solution was concentrated under reduced pressure. The sameprocedure as a series of reactions of Example 17 was carried out, usingthe obtained compound and N,N-dipropyl-1,4-butanediamine, to obtain thetitle compound (76.6 mg) having the following physical properties.

Description: amorphous;

TLC: Rf 0.67 (chloroform:methanol: 28% ammonia water=80:20:4);

NMR (DMSO-D₆): δ d 0.81 (t, J=7.5 Hz, 6H), 1.22-1.60 (m, 8H), 2.13-2.43(m, 6H), 3.22 (q, J=6.6 Hz, 2H), 4.59 (s, 4H), 6.73-6.98 (m, 2H),6.98-7.24 (m, 2H), 7.50 (d, J=9.0 Hz, 2H), 7.74 (d, J=9.0 Hz, 2H), 8.25(t, J=5.7 Hz, 1H), 9.57 (s, 1H), 11.99-12.39 (m, 2H)

Example 494-[(2-cyclohexyl-2,7-diazaspiro[3.5]nona-7-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 49)

The same procedure as a series of reactions of Example 43 was carriedout, using the compound 41 and 2-cyclohexyl-2,7-diazaspiro[3.5]nonane,to obtain the title compound having the following physical properties.

MS: 502 (M+H)⁺; HPLC retention time: 2.74 minutes.

Example 49(1) to Example 49(20)

The same operation as a series of reactions of Example 49 was carriedout, except that a corresponding amine compound was used in place of2-cyclohexyl-2,7-diazaspiro[3.5]nonane, to obtain the title compoundhaving the following physical properties.

Example 49(1)4-[(2-cyclohexyl-2,8-diazaspiro[4.5]dec-8-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 49-1)

MS: 516 (M+H)⁺; HPLC retention time: 2.40 minutes.

Example 49(2)4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 49-2)

MS: 516 (M+H)⁺; HPLC retention time: 2.41 minutes

Example 49(3)4-[(9-cyclohexyl-2,9-diazaspiro[5.5]undec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 49-3)

MS: 530 (M+H)⁺; HPLC retention time: 2.46 minutes

Example 49(4)4-[(7-cyclohexyl-2,7-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 49-4)

MS: 516 (M+H)⁺; HPLC retention time: 2.43 minutes

Example 49(5)4-[(8-cyclohexyl-2,8-diazaspiro[5.5]undec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 49-5)

MS: 530 (M+H)⁺; HPLC retention time: 2.46 minutes

Example 49(6)4-[(1′-cyclohexylspiro[indole-3,4′-piperidine]-1(2H)-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 49-6)

MS: 564 (M+H)⁺; HPLC retention time: 2.80 minutes

Example 49(7)4-[(1′-cyclohexyl-5-methylspiro[indole-3,4′-piperidine]-1(2H)-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 49-7)

MS: 578 (M+H)⁺; HPLC retention time: 2.88 minutes

Example 49(8)4-[(1′-cyclohexyl-5-fluorospiro[indole-3,4′-piperidine]-1(2H)-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 49-8)

MS: 582 (M+H)⁺; HPLC retention time: 2.84 minutes

Example 49(9) 4-[(5-chloro1′-cyclohexylspiro[indole-3,4′-piperidine]-1(2H)-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 49-9)

MS: 598 (M+H)⁺; HPLC retention time: 2.93 minutes

Example 49(10)4-[(1-cyclohexyl-1,2-dihydro-1′H-spiro[indole-3,4′-piperidine]-1′-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 49-10)

MS: 564 (M+H)⁺; HPLC retention time: 2.95 minutes

Example 49(11)4-{[3-(dipropylamino)-2,3-dihydro-1′H-spiro[indene-1,4′-piperidine]-1′-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 49-11)

MS: 580 (M+H)⁺; HPLC retention time: 2.59 minutes

Example 49(12)4-[(1-cyclohexyl-5-methyl-1,2-dihydro-1′H-spiro[indole-3,4′-piperidine]-1′-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 49-12)

MS: 576 (M+H)⁺; HPLC retention time: 2.76 minutes

Example 49(13)4-[(4-cyclohexyl-1-oxa-4,9-diazaspiro[5.5]undec-9-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 49-13)

MS: 532 (M+H)⁺; HPLC retention time: 2.39 minutes

Example 49(14)4-[(9-cyclohexyl-1-oxa-4,9-diazaspiro[5.5]undec-4-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 49-14)

MS: 532 (M+H)⁺; HPLC retention time: 2.47 minutes

Example 49(15)4-[(4-cyclohexyl-1-oxa-4,8-diazaspiro[5.5]undec-8-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 49-15)

MS: 532 (M+H)⁺; HPLC retention time: 2.41 minutes

Example 49(16)4-[(8-cyclohexyl-1-oxa-4,8-diazaspiro[5.5]undec-4-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 49-16)

MS: 532 (M+H)⁺; HPLC retention time: 2.41 minutes

Example 49(17)4-[(2-cyclohexyl-2,3-dihydro-1H,1′H-spiro[isoquinoline-4,4′-piperidine]-1′-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 49-17)

MS: 578 (M+H)⁺; HPLC retention time: 2.60 minutes

Example 49(18)4-[(1′-cyclohexyl-1H-spiro[isoquinoline-4,4′-piperidine]-2(3H)-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 49-18)

MS: 578 (M+H)⁺; HPLC retention time: 2.54 minutes

Example 49(19) ethyl8-(4-{[bis(1H-imidazol-2-ylmethyl)amino]carbonyl}benzyl)-2-cyclohexyl-2,8-diazaspiro[4.5]decane-3-carboxylate(Compound 49-19)

MS: 588 (M+H)⁺; HPLC retention time: 2.57 minutes

Example 49(20)4-{[4-(dipropylamino)-3,4-dihydro-1′H-spiro[chromene-2,4′-piperidine]-1′-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 49-20)

MS: 596 (M+H)⁺; HPLC retention time: 2.61 minutes

Example 50(1) to Example 50(147)

The same procedure as a series of reactions of Example 43 was carriedout, except that a corresponding aldehyde was used in place of thecompound 41 and a corresponding amine was used in place of the compound42, to obtain the title compound having the following physicalproperties.

Example 50(1)4-[({trans-4-[benzyl(propyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide

Description: amorphous;

TLC: Rf 0.48 (ethyl acetate: methanol: 28% ammonia water=90:10:2);

NMR (CDCl₃): δ d 0.81 (t, J=7.2 Hz, 3H), 1.02-1.42 (m, 6H), 1.78-1.90(m, 2H), 1.95-2.06 (m, 2H), 2.40 (t, J=7.2 Hz, 2H), 2.40-2.60 (m, 2H),3.59 (s, 2H), 3.81 (s, 2H), 4.50-4.75 (m, 4H), 6.96-7.10 (m, 4H),7.15-7.40 (m, 7H), 7.50-7.60 (m, 2H)

Example 50(2)4-[({trans-4-[cyclohexyl(ethyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-2)

Description: amorphous;

TLC: Rf 0.21 (ethyl acetate: methanol: 28% ammonia water=90:10:2)

NMR (CDCl₃): δ d 0.98-2.05 (m, 21H), 2.40 (m, 1H), 2.50-2.75 (m, 4H),3.81 (s, 2H), 4.58-4.75 (m, 4H), 6.98-7.10 (m, 4H), 7.35 (d, J=8.1 Hz,2H), 7.59 (d, J=8.1 Hz, 2H)

Example 50(3)4-[(7-cyclohexyl-2,7-diazaspiro[4.4]nona-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-3)

Description: amorphous;

TLC: Rf 0.65 (chloroform:methanol: 28% ammonia water=40:10:2);

NMR (CDCl₃): δ 1.00-1.45 (m, 6H), 1.53-2.10 (m, 8H), 2.19-2.41 (m, 2H),2.47-3.00 (m, 7H), 3.53-3.68 (m, 2H), 4.48-4.88 (m, 4H), 6.95-7.09 (m,4H), 7.33 (d, J=8.1 Hz, 2H), 7.53 (d, J=8.1 Hz, 2H)

Example 50(4)4-[(2-cyclohexyl-2,7-diazaspiro[4.5]dec-7-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-4)

Description: amorphous;

TLC: Rf 0.72 (chloroform:methanol: 28% ammonia water=40:10:2);

NMR (CDCl₃): δ 0.94-1.30 (m, 4H), 1.30-2.57 (m, 19H), 2.56-2.91 (m, 2H),3.24-3.51 (m, 2H), 4.40-4.92 (m, 4H), 7.01 (s, 4H), 7.33 (d, J=8.1 Hz,2H), 7.50 (d, J=8.1 Hz, 2H)

Example 50(5)4-({[4-(3,4-dihydroisoquinoline-2(1H)-yl)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Low Polar Compound) (Compound 50-5)

Description: amorphous;

TLC: Rf 0.55 (ethyl acetate: methanol: 28% ammonia water=90:10:2);

NMR (CDCl₃): δ d 1.44-1.92 (m, 8H), 2.48 (m, 1H), 2.75-2.95 (m, 5H),3.78 (s, 2H), 3.79 (s, 2H), 4.55-4.72 (m, 4H), 6.98-7.18 (m, 8H), 7.40(d, J=7.8 Hz, 2H), 7.63 (d, J=7.8 Hz, 2H)

Example 50(6)4-({[4-(3,4-dihydro-2(1H)-isoquinolinyl)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(High Polar Compound) (Compound 50-6)

Description: amorphous;

TLC: Rf 0.41 (ethyl acetate: methanol: 28% ammonia water=90:10:2);

NMR (CDCl₃): δ d 1.15-2.60 (m, 10H), 2.80-2.98 (m, 4H), 3.79 (s, 2H),3.86 (s, 2H), 4.55-4.75 (m, 4H), 6.98-7.15 (m, 8H), 7.39 (d, J=8.4 Hz,2H), 7.63 (d, J=8.4 Hz, 2H)

Example 50(7)N,N-bis(1H-imidazol-2-ylmethyl)-4-[({4-[(4aS,8aR)-octahydroisoquinoline-2(1H)-yl]cyclohexyl}amino)methyl]benzamide(Low Polar Compound) (Compound 50-7)

Description: amorphous;

TLC: Rf 0.50 (ethyl acetate:methanol: 28% ammonia water=90:10:2);

NMR (CDCl₃): δ d 0.80-2.82 (m, 24H), 2.94 (m, 1H), 3.12 (m, 1H), 3.75(s, 2H), 4.52-4.72 (m, 4H), 6.98-7.18 (m, 4H), 7.39 (d, J=8.4 Hz, 2H),7.63 (d, J=8.4 Hz, 2H)

Example 50(8)4-[({4-[(4aS,8aR)-octahydro-2(1H)-isoquinolinyl]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(High Polar Compound) (Compound 50-8)

Description: amorphous;

TLC: Rf 0.37 (ethyl acetate: methanol: 28% ammonia water=90:10:2);

NMR (CDCl₃): δ d 0.80-2.50 (m, 24H), 2.90 (m, 1H), 2.98 (m, 1H), 3.81(s, 2H), 4.52-4.75 (m, 4H), 6.98-7.18 (m, 4H), 7.36 (d, J=8.4 Hz, 2H),7.63 (d, J=8.4 Hz, 2H)

Example 50(9)4-[({trans-4-[benzyl(cyclohexyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-9)

Description: amorphous;

TLC: Rf 0.54 (ethyl acetate: methanol: 28% ammonia water=90:10:2);

NMR (CDCl₃): δ d 0.95-2.65 (m, 21H), 3.72 (s, 2H), 3.80 (s, 2H),4.52-4.75 (m, 4H), 6.98-7.15 (m, 4H), 7.18-7.40 (m, 7H), 7.59 (d, J=8.1Hz, 2H)

Example 50(10)N,N-bis(1H-imidazol-2-ylmethyl)-4-{[(cis-4-morpholine-4-ylcyclohexyl)amino]methyl}benzamide(Compound 50-10)

Description: amorphous;

TLC: Rf 0.62 (chloroform:methanol: 28% ammonia water=80:20:4);

NMR (DMSO-D₆): δ d 1.26-1.47 (m, 4H), 1.53-1.75 (m, 4H), 2.00-2.15 (m,1H), 2.32-2.45 (m, 4H), 2.54-2.64 (m, 1H), 3.48-3.61 (m, 4H), 3.67 (s,2H), 4.47-4.68 (m, 4H), 6.76-7.00 (m, 2H), 7.00-7.15 (m, 2H), 7.33 (d,J=8.4 Hz, 2H), 7.42 (d, J=8.4 Hz, 2H), 11.95-12.24 (m, 1H), 12.25-12.56(m, 1H)

Example 50(11)N,N-bis(1H-imidazol-2-ylmethyl)-4-{[(trans-4-morpholine-4-ylcyclohexyl)amino]methyl}benzamide(Compound 50-11)

Description: amorphous;

TLC: Rf 0.50 (chloroform:methanol: 28% ammonia water=80:20:4);

NMR (DMSO-D₆): δ 0.86-1.26 (m, 4H), 1.66-1.82 (m, 2H), 1.84-1.99 (m,2H), 2.04-2.18 (m, 1H), 2.19-2.33 (m, 1H), 2.34-2.45 (m, 4H), 3.44-3.58(m, 4H), 3.70 (s, 2H), 4.42-4.69 (m, 4H), 6.76-6.98 (m, 2H), 6.98-7.17(m, 2H), 7.31 (d, J=8.4 Hz, 2H), 7.41 (d, J=8.4 Hz, 2H), 11.99-12.25 (m,1H), 12.26-12.50 (m, 1H)

Example 50(12) N,N-bis(1H-imidazol-2-ylmethyl)-4-{[(4-piperidine1-ylcyclohexyl)amino]methyl}benzamide (Low Polar Compound) (Compound50-12)

Description: amorphous;

TLC: Rf 0.21 (ethyl acetate: methanol: 28% ammonia water=80:20:4);

NMR (CDCl₃): δ d 1.10-2.10 (m, 14H), 2.88-3.10 (m, 6H), 3.73 (s, 2H),4.55-4.78 (m, 4H), 6.98-7.10 (m, 4H), 7.35 (d, J=8.1 Hz, 2H), 7.55 (d,J=8.1 Hz, 2H)

Example 50(13)N,N-bis(1H-imidazol-2-ylmethyl)-4-({[4-(1-piperidinyl)cyclohexyl]amino}methyl)benzamide(High Polar Compound) (Compound 50-13)

Description: amorphous;

TLC: Rf 0.15 (ethyl acetate: methanol: 28% ammonia water=80:20:4);

NMR (CDCl₃): δ d 1.10-1.30 (m, 2H), 1.38-1.65 (m, 4H), 1.78-1.95 (m,4H), 2.00-2.20 (m, 4H), 2.43 (m, 1H), 2.70-3.00 (m, 5H), 3.82 (s, 2H),4.55-4.78 (m, 4H), 6.98-7.10 (m, 4H), 7.33 (d, J=8.1 Hz, 2H), 7.53 (d,J=8.1 Hz, 2H)

Example 50(14)N,N-bis(1H-imidazol-2-ylmethyl)-4-({[4-(4-methylpiperazine-1-yl)cyclohexyl]amino}methyl)benzamide(Low Polar Compound) (Compound 50-14)

Description: amorphous;

TLC: Rf 0.22 (ethyl acetate: methanol: 28% ammonia water=80:20:4);

NMR (CDCl₃): δ d 1.45-1.90 (m, 8H), 2.28-2.90 (m, 10H), 2.37 (s, 3H),3.79 (s, 2H), 4.55-4.78 (m, 4H), 6.98-7.12 (m, 4H), 7.39 (d, J=7.8 Hz,2H), 7.53 (d, J=7.8 Hz, 2H)

Example 50(15)N,N-bis(1H-imidazol-2-ylmethyl)-4-({[4-(4-methyl-1-piperazinyl)cyclohexyl]amino}methyl)benzamide.(High Polar Compound) (Compound 50-15)

Description: amorphous;

TLC: Rf 0.19 (ethyl acetate: methanol: 28% ammonia water=80:20:4);

NMR (CDCl₃): δ d 1.20-1.48 (m, 4H), 1.99 (m, 2H), 2.15 (m, 2H), 2.34 (s,3H), 2.38 (m, 1H), 2.45-2.80 (m, 9H), 3.88 (s, 2H), 4.50-4.78 (m, 4H),7.03 (s, 4H), 7.40 (s, 4H)

Example 50(16) 4-({[4-(4,4-difluoropiperidine1-yl)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Low Polar Compound) (Compound 50-16)

Description: amorphous;

TLC: Rf 0.69 (ethyl acetate: methanol: 28% ammonia water=80:20:4);

NMR (CDCl₃): δ d 1.45-2.10 (m, 12H), 2.40 (m, 1H), 2.60-2.74 (m, 4H),2.92 (m, 1H), 3.84 (s, 2H), 4.55-4.78 (m, 4H), 7.05 (s, 4H), 7.38-7.50(m, 4H)

Example 50(17)4-({[4-(4,4-difluoro-1-piperidinyl)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(High Polar Compound) (Compound 50-17)

Description: amorphous;

TLC: Rf 0.55 (ethyl acetate: methanol: 28% ammonia water=80:20:4);

NMR (CDCl₃): δ d 1.23-1.42 (m, 4H), 1.82-2.20 (m, 8H), 2.42 (m, 1H),2.60 (m, 1H), 2.60-2.70 (m, 4H), 3.87 (s, 2H), 4.52-4.76 (m, 4H), 7.04(s, 4H), 7.35-7.48 (m, 4H)

Example 50(18)4-{[(4-azepan-1-ylcyclohexyl)amino]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Low Polar Compound) (Compound 50-18)

Description: amorphous;

TLC: Rf 0.52 (ethyl acetate:methanol: 28% ammonia water=80:20:4);

NMR (CDCl₃): δ d 1.40-1.95 (m, 16H), 2.75-3.10 (m, 6H), 3.73 (s, 2H),4.55-4.78 (m, 4H), 7.00-7.10 (m, 4H), 7.35 (d, J=8.4 Hz, 2H), 7.55 (d,J=8.4 Hz, 2H)

Example 50(19)4-({[4-(1-azepanyl)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(High Polar Compound) (Compound 50-19)

Description: amorphous;

TLC: Rf 0.29 (ethyl acetate: methanol: 28% ammonia water=80:20:4);

NMR (CDCl₃): δ d 1.10-1.25 (m, 2H), 1.32-1.52 (m, 2H), 1.60-2.20 (m,12H), 2.41 (m, 1H), 2.70-3.22 (m, 5H), 3.81 (s, 2H), 4.55-4.78 (m, 4H),6.98-7.10 (m, 4H), 7.33 (d, J=8.4 Hz, 2H), 7.56 (d, J=8.4 Hz, 2H)

Example 50(20)4-({[4-(dibenzylamino)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Low Polar Compound) (Compound 50-20)

Description: amorphous;

TLC: Rf 0.52 (ethyl acetate: methanol: 28% ammonia water=90:10:2);

NMR (CDCl₃): δ d 1.30-2.00 (m, 8H), 2.52 (m, 1H), 2.90 (m, 1H),3.55-3.70 (m, 4H), 3.81 (s, 2H), 4.55-4.78 (m, 4H), 7.01 (s, 4H),7.15-7.42 (m, 14H)

Example 50(21)4-({[4-(dibenzylamino)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(High Polar Compound) (Compound 50-21)

Description: amorphous;

TLC: Rf 0.40 (ethyl acetate: methanol: 28% ammonia water=90:10:2);

NMR (CDCl₃): δ d 1.00-1.20 (m, 2H), 1.30-1.50 (m, 2H), 1.82-1.98 (m,2H), 1.98-2.10 (m, 2H), 2.40-2.60 (m, 2H), 3.60 (s, 4H), 3.81 (s, 2H),4.50-4.76 (m, 4H), 6.90-7.08 (m, 4H), 7.18-7.40 (m, 12H), 7.47 (d, J=8.1Hz, 2H)

Example 50(22)4-({[trans-4-(cyclohexylamino)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-22)

Description: amorphous;

TLC: Rf 0.41 (dichloromethane:methanol: 28% ammonia water=90:10:2);

NMR (CDCl₃): δ d 1.02-2.12 (m, 18H), 2.44 (m, 1H), 2.65-2.82 (m, 2H),3.81 (s, 2H), 4.56-4.80 (m, 4H), 6.95-7.10 (m, 4H), 7.33 (d, J=8.1 Hz,2H), 7.53 (d, J=8.1 Hz, 2H)

Example 50(23)3-({[4-(dipropylamino)butyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-23)

Description: oily product;

TLC: Rf 0.35 (dichloromethane:methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 0.85 (t, J=7.5 Hz, 6H), 1.39-1.49 (m, 8H), 2.33-2.43 (m,6H), 2.61-2.66 (m, 2H), 3.79 (s, 2H), 4.51 (br-s, 2H), 4.73 (br-s, 2H),7.05 (br, 4H), 7.36-7.38 (m, 2H), 7.54 (m, 1H), 7.62 (s, 1H)

Example 50(24)4-[(9-cyclohexyl-3,9-diazaspiro[5.5]undec-3-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-24)

Description: amorphous;

TLC: Rf 0.37 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 0.97-1.34 (m, 5H), 1.37-1.69 (m, 9H), 1.71-1.98 (m, 4H),2.24-2.42 (m, 5H), 2.45-2.64 (m, 4H), 3.48 (s, 2H), 4.53-4.85 (m, 4H),6.86-7.13 (m, 4H), 7.33 (d, J=8.1 Hz, 2H), 7.48 (d, J=8.1 Hz, 2H)

Example 50(25)4-{[8-(cyclohexylmethyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-25)

Description: amorphous;

TLC: Rf 0.73 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 0.78-0.92 (m, 2H), 1.10-1.30 (m, 3H), 1.40-1.79 (m, 12H),2.07 (d, J=7.2 Hz, 2H), 2.22-2.36 (m, 6H), 2.54 (t, J=6.9 Hz, 2H), 3.58(s, 2H), 4.59-4.68 (m, 4H), 6.95-7.10 (m, 4H), 7.38 (d, J=8.4 Hz, 2H),7.64 (d, J=8.4 Hz, 2H)

Example 50(26)4-[(8-benzyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-26)

Description: amorphous;

TLC: Rf 0.73 (ethyl acetate:methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 1.53-1.64 (m, 6H), 2.27-2.41 (m, 6H), 2.55 (t, J=6.9 Hz,2H), 3.46 (s, 2H), 3.58 (s, 2H), 4.60-4.68 (m, 4H), 6.96-7.08 (m, 4H),7.20-7.32 (m, 5H), 7.37 (d, J=8.4 Hz, 2H), 7.64 (d, J=8.4 Hz, 2H)

Example 50(27)4-{[8-(cyclopropylmethyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-27)

Description: amorphous;

TLC: Rf 0.42 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 0.06-0.14 (m, 2H), 0.46-0.54 (m, 2H), 0.87 (m, 1H),1.56-1.67 (m, 6H), 2.23 (d, J=6.6 Hz, 2H), 2.34 (s, 2H), 2.34-2.54 (m,4H), 2.56 (t, J=6.9 Hz, 2H), 3.58 (s, 2H), 4.58-4.65 (m, 4H), 6.94-7.10(m, 4H), 7.38 (d, J=8.4 Hz, 2H), 7.66 (d, J=8.4 Hz, 2H)

Example 50(28)4-{[8-(cyclopentylmethyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-28)

Description: amorphous;

TLC: Rf 0.59 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 1.09-1.22 (m, 2H), 1.44-1.65 (m, 9H), 1.68-1.80 (m, 2H),1.98-2.10 (m, 2H), 2.23 (d, J=7.2 Hz, 2H), 2.28-2.40 (m, 6H), 2.55 (t,J=6.9 Hz, 2H), 3.58 (s, 2H), 4.58-4.65 (m, 4H), 6.94-7.10 (m, 4H), 7.38(d, J=8.4 Hz, 2H), 7.65 (d, J=8.4 Hz, 2H)

Example 50(29)N,N-bis(1H-imidazol-2-ylmethyl)-4-{[8-(2-thienylmethyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}benzamide(Compound 50-29)

Description: amorphous;

TLC: Rf 0.60 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 1.53-1.65 (m, 6H), 2.32-2.47 (m, 6H), 2.55 (t, J=6.9 Hz,2H), 3.58 (s, 2H), 3.67 (s, 2H), 4.58-4.65 (m, 4H), 6.88 (d, J=3.0 Hz,1H), 6.93 (dd, J=4.5, 3.0 Hz, 1H), 6.98-7.10 (m, 4H), 7.20 (d, J=4.5 Hz,1H), 7.38 (d, J=8.4 Hz, 2H), 7.68 (d, J=8.4 Hz, 2H)

Example 50(30)N,N-bis(1H-imidazol-2-ylmethyl)-4-{[8-(3-thienylmethyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}benzamide(Compound 50-30)

Description: amorphous;

TLC: Rf 0.60 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 1.53-1.65 (m, 6H), 2.28-2.42 (m, 6H), 2.55 (t, J=6.9 Hz,2H), 3.49 (s, 2H), 3.58 (s, 2H), 4.58-4.65 (m, 4H), 6.96-7.14 (m, 6H),7.25 (m, 1H), 7.38 (d, J=8.4 Hz, 2H), 7.68 (d, J=8.4 Hz, 2H)

Example 50(31)4-({[4-(3,4-dihydroquinoline-1(2H)-yl)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Low Polar Compound) (Compound 50-31)

Description: amorphous;

TLC: Rf 0.46 (ethyl acetate: methanol: 28% ammonia water=90:10:2);

NMR (CDCl₃): δ d 1.50-1.75 (m, 4H), 1.82-2.10 (m, 6H), 2.71 (t, J=6.3Hz, 2H), 3.00 (m, 1H), 3.24 (t, J=5.4 Hz, 2H), 3.60 (m, 1H), 3.83 (s,2H), 4.58-4.80 (m, 4H), 6.53 (dd, J=7.8, 7.8 Hz, 1H), 6.62 (d, J=7.8 Hz,1H), 6.93 (d, J=7.5 Hz, 1H), 6.99-7.12 (m, 5H), 7.45 (d, J=8.1 Hz, 2H),7.54 (d, J=8.1 Hz, 2H)

Example 50(32)4-({[4-(3,4-dihydro-1(2H)-quinolinyl)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(High Polar Compound) (Compound 50-32)

Description: amorphous;

TLC: Rf 0.30 (ethyl acetate: methanol: 28% ammonia water=90:10:2);

NMR (CDCl₃): δ d 1.22-2.20 (m, 10H), 2.55 (m, 1H), 2.71 (t, J=6.0 Hz,2H), 3.15 (t, J=5.7 Hz, 2H), 3.63 (m, 1H), 3.87 (s, 2H), 4.55-4.78 (m,4H), 6.54 (dd, J=7.5, 7.5 Hz, 1H), 6.62 (d, J=7.5 Hz, 1H), 6.93 (d,J=7.5 Hz, 1H), 7.00-7.12 (m, 5H), 7.36-7.45 (m, 2H), 7.50-7.62 (m, 2H)

Example 50(33)4-({8-[4-(diethylamino)benzyl]-2,8-diazaspiro[4.5]dec-2-yl}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-33)

Description: amorphous;

TLC: Rf 0.44 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 1.14 (t, J=7.2 Hz, 6H), 1.51-1.64 (m, 6H), 2.25-2.42 (m,6H), 2.53 (t, J=6.9 Hz, 2H), 3.28-3.38 (m, 6H), 3.56 (s, 2H), 4.60-4.67(m, 4H), 6.61 (d, J=8.7 Hz, 2H), 6.94-7.08 (m, 4H), 7.12 (d, J=8.7 Hz,2H), 7.36 (d, J=8.4 Hz, 2H), 7.59 (d, J=8.4 Hz, 2H)

Example 50(34)4-{[8-(2-fluorobenzyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-34)

Description: amorphous;

TLC: Rf 0.44 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 1.56-1.64 (m, 6H), 2.32-2.44 (m, 6H), 2.54 (t, J=6.9 Hz,2H), 3.54 (s, 2H), 3.58 (s, 2H), 4.60-4.68 (m, 4H), 6.97-7.13 (m, 6H),7.25 (m, 1H), 7.38-7.42 (m, 3H), 7.61 (d, J=8.4 Hz, 2H)

Example 50(35)4-{[8-(3-fluorobenzyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-35)

Description: amorphous;

TLC: Rf 0.44 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 1.50-1.66 (m, 6H), 2.24-2.41 (m, 6H), 2.56 (t, J=6.9 Hz,2H), 3.43 (s, 2H), 3.59 (s, 2H), 4.60-4.68 (m, 4H), 6.90 (m, 1H),6.95-7.09 (m, 6H), 7.23 (m, 1H), 7.36 (d, J=8.4 Hz, 2H), 7.60 (d, J=8.4Hz, 2H)

Example 50(36)4-{[8-(4-fluorobenzyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-36)

Description: amorphous;

TLC: Rf 0.44 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 1.52-1.62 (m, 6H), 2.24-2.41 (m, 6H), 2.55 (t, J=6.9 Hz,2H), 3.41 (s, 2H), 3.58 (s, 2H), 4.60-4.68 (m, 4H), 6.93-7.09 (m, 6H),7.20-7.28 (m, 2H), 7.35 (d, J=8.4 Hz, 2H), 7.60 (d, J=8.4 Hz, 2H)

Example 50(37)4-[({trans-4-[acetyl(cyclohexyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-37)

Description: amorphous;

TLC: Rf 0.50 (ethyl acetate: methanol: 28% ammonia water=80:20:2);

NMR (CDCl₃): δ d 1.05-2.62 (m, 19H), 2.06 (s, 3H), 3.00 (brs, 1H), 3.39(m, 1H), 3.82 (s, 2H), 4.50-4.80 (m, 4H), 6.95-7.12 (m, 4H), 7.30-7.40(m, 2H), 7.45-7.60 (m, 2H)

Example 50(38)N-(1H-benzoimidazol-2-ylmethyl)-4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N-(1H-imidazol-2-ylmethyl)benzamide(Compound 50-38)

Description: amorphous;

TLC: Rf 0.34 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.12-1.26 (m, 5H), 1.53-1.65 (m, 7H), 1.74-1.89 (m, 4H),2.23-2.33 (m, 3H), 2.44-2.57 (m, 6H), 3.55 (s, 2H), 4.67-4.81 (m, 2H),4.83-4.91 (m, 2H), 6.99-7.08 (m, 2H), 7.23-7.37 (m, 4H), 7.49-7.64 (m,4H)

Example 50(39)N-(1H-benzoimidazol-2-ylmethyl)-4-[({trans-4-[cyclohexyl(propyl)amino]cyclohexyl}amino)methyl]-N-(1H-imidazol-2-ylmethyl)benzamide(Compound 50-39)

Description: amorphous;

TLC: Rf 0.38 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 0.76-0.88 (m, 3H), 1.09-1.15 (m, 2H), 1.17-1.27 (m, 5H),1.29-1.41 (m, 4H), 1.56-1.63 (m, 1H), 1.67-1.80 (m, 6H), 1.90-2.03 (m,2H), 2.34-2.47 (m, 3H), 2.50-2.63 (m, 2H), 3.79 (s, 2H), 4.62-4.69 (m,1H), 4.72-4.79 (m, 1H), 4.84 (s, 2H), 6.97-7.11 (m, 2H), 7.22-7.35 (m,4H), 7.49-7.65 (m, 4H)

Example 50(40)N,N-bis(1H-imidazol-2-ylmethyl)-4-[({trans-4-[(3-methoxypropyl)(propyl)amino]cyclohexyl}amino)methyl]benzamide(Compound 50-40)

Description: oily product;

TLC: Rf 0.49 (ethyl acetate: methanol: 28% ammonia water=40:10:1);

NMR (CDCl₃): δ 0.84 (t, J=7.5 Hz, 3H), 1.08-2.02 (m, 12H), 2.33-2.50 (m,6H), 3.31 (s, 3H), 3.39 (t, J=6.0 Hz, 2H), 3.82 (s, 2H), 4.56 (br-s,2H), 4.60 (br-s, 2H), 7.01 (br-s, 2H), 7.08 (br-s, 2H), 7.37 (d, J=7.5Hz, 2H), 7.29 (d, J=7.5 Hz, 2H)

Example 50(41)4-[({trans-4-[(1-ethylpropyl)(propyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-41)

Description: oily product;

TLC: Rf 0.69 (ethyl acetate:methanol: 28% ammonia water=40:10:1);

NMR (CDCl₃): δ 0.82 (t, J=7.5 Hz, 3H), 0.86 (t, J=7.5 Hz, 6H), 1.10-2.02(m, 14H), 2.17-2.49 (m, 5H), 3.82 (s, 2H), 4.59 (br-s, 2H), 4.63 (br-s,2H), 7.02 (br-s, 2H), 7.08 (br-s, 2H), 7.37 (d, J=8.1 Hz, 2H), 7.66 (d,J=8.1 Hz, 2H)

Example 50(42)4-{[[4-(dipropylamino)butyl](methyl)amino]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-42)

Description: oily product;

TLC: Rf 0.58 (ethyl acetate: methanol: 28% ammonia water=40:10:1);

NMR (CDCl₃): δ 0.86 (t, J=7.5 Hz, 6H), 1.41-1.50 (m, 8H), 2.16 (s, 3H),2.33-2.44 (m, 8H), 3.47 (s, 2H), 4.61 (br-s, 2H), 4.65 (br-s, 2H), 7.02(br-s, 2H), 7.07 (br-s, 2H), 7.37 (d, J=8.1 Hz, 2H), 7.64 (d, J=8.1 Hz,2H)

Example 50(43)4-[(8-cyclopentyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-43)

Description: amorphous;

TLC: Rf 0.39 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.24-1.74 (m, 12H), 1.75-1.94 (m, 2H), 2.19-2.49 (m, 7H),2.54 (t, J=6.95 Hz, 2H), 3.57 (s, 2H), 4.54-4.81 (m, 4H), 6.88-7.13 (m,4H), 7.35 (d, J=8.1 Hz, 2H), 7.54 (d, J=8.1 Hz, 2H)

Example 50(44)N,N-bis(1H-imidazol-2-ylmethyl)-4-{[8-(tetrahydro-2H-pyran-4-yl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}benzamide(Compound 50-44)

Description: amorphous;

TLC: Rf 0.33 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.44-1.67 (m, 8H), 1.67-1.81 (m, 2H), 2.24-2.63 (m, 9H),3.25-3.43 (m, 2H), 3.57 (s, 2H), 3.92-4.08 (m, 2H), 4.53-4.77 (m, 4H),6.91-7.13 (m, 4H), 7.36 (d, J=8.1 Hz, 2H), 7.55 (d, J=8.1 Hz, 2H)

Example 50(45)N,N-bis(1H-imidazol-2-ylmethyl)-4-{[8-(pyridine-2-ylmethyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}benzamide(Compound 50-45)

Description: amorphous;

TLC: Rf 0.46 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 1.54-1.65 (m, 6H), 2.32-2.48 (m, 6H), 2.55 (t, J=6.9 Hz,2H), 3.58 (s, 2H), 3.61 (s, 2H), 4.60-4.72 (m, 4H), 6.94-7.09 (m, 4H),7.14 (dd, J=4.8, 7.5 Hz, 1H), 7.35 (d, J=8.4 Hz, 2H), 7.40 (d, J=7.5 Hz,1H), 7.53 (d, J=8.4 Hz, 2H), 7.63 (dt, J=1.8, 7.5 Hz, 1H), 8.53 (d,J=4.8 Hz, 1H)

Example 50(46)N,N-bis(1H-imidazol-2-ylmethyl)-4-{[8-(pyridine-3-ylmethyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}benzamide(Compound 50-46)

Description: amorphous;

TLC: Rf 0.46 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 1.50-1.64 (m, 6H), 2.24-2.41 (m, 6H), 2.56 (t, J=6.9 Hz,2H), 3.46 (s, 2H), 3.58 (s, 2H), 4.61-4.72 (m, 4H), 6.98-7.10 (m, 4H),7.23 (dd, J=4.8, 7.5 Hz, 1H), 7.36 (d, J=8.4 Hz, 2H), 7.54 (d, J=8.4 Hz,2H), 7.65 (dt, J=7.5, 1.8 Hz, 1H), 8.49 (dd, J=1.8, 4.8 Hz, 1H), 8.51(d, J=1.8 Hz, 1H)

Example 50(47)N,N-bis(1H-imidazol-2-ylmethyl)-4-{[8-(pyridine-4-ylmethyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}benzamide(Compound 50-47)

Description: amorphous;

TLC: Rf 0.46 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 1.55-1.68 (m, 6H), 2.24-2.42 (m, 6H), 2.56 (t, J=6.9 Hz,2H), 3.44 (s, 2H), 3.58 (s, 2H), 4.60-4.72 (m, 4H), 6.97-7.08 (m, 4H),7.23 (d, J=6.0 Hz, 2H), 7.34 (d, J=8.4 Hz, 2H), 7.52 (d, J=8.4 Hz, 2H),8.50 (d, J=6.0 Hz, 2H)

Example 50(48)N,N-bis(1H-imidazol-2-ylmethyl)-4-{[8-(2-naphthylmethyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}benzamide(Compound 50-48)

Description: amorphous;

TLC: Rf 0.52 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 1.56-1.65 (m, 6H), 2.32-2.48 (m, 6H), 2.58 (t, J=6.9 Hz,2H), 3.57 (s, 2H), 3.62 (s, 2H), 4.60-4.70 (m, 4H), 6.97-7.08 (m, 4H),7.35 (d, J=8.4 Hz, 2H), 7.40-7.50 (m, 3H), 7.54 (d, J=8.4 Hz, 2H), 7.71(s, 1H), 7.74-7.82 (m, 3H)

Example 50(49)4-[(8-cycloheptyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-49)

Description: amorphous;

TLC: Rf 0.32 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.27-1.86 (m, 18H), 2.23-2.63 (m, 9H), 3.57 (s, 2H),4.51-4.75 (m, 4H), 6.90-7.11 (m, 4H), 7.36 (d, J=8.1 Hz, 2H), 7.59 (d,J=8.1 Hz, 2H)

Example 50(50)4-[({trans-4-[cyclohexyl(methyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-50)

Description: amorphous;

TLC: Rf 0.43 (ethyl acetate: methanol: 28% ammonia water=80:20:2);

NMR (CDCl₃): δ d 1.00-2.06 (m, 18H), 2.30 (s, 3H), 2.42 (m, 1H),2.55-2.75 (m, 2H), 3.81 (s, 2H), 4.52-4.75 (m, 4H), 6.95-7.10 (m, 4H),7.35 (d, J=8.4 Hz, 2H), 7.61 (d, J=8.4 Hz, 2H)

Example 50(51)4-[({trans-4-[benzyl(methyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-51)

Description: amorphous;

TLC: Rf 0.24 (ethyl acetate: methanol: 28% ammonia water=90:10:2);

NMR (CDCl₃): δ d 1.15-1.50 (m, 4H), 1.90-2.18 (m, 4H), 2.21 (s, 3H),2.45-2.62 (m, 2H), 3.60 (s, 2H), 3.85 (s, 2H), 4.50-4.78 (m, 4H),6.98-7.12 (m, 5H), 7.20-7.40 (m, 4H), 7.38 (d, J=8.4 Hz, 2H), 7.55 (d,J=8.4 Hz, 2H)

Example 50(52)4-[({trans-4-[cyclohexyl(methylsulfonyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-52)

Description: amorphous;

TLC: Rf 0.22 (ethyl acetate: methanol: 28% ammonia water=90:10:2);

NMR (CDCl₃): δ d 1.00-2.12 (m, 18H), 2.49 (m, 1H), 2.86 (s, 3H),3.10-3.42 (m, 2H), 3.83 (s, 2H), 4.58-4.75 (m, 4H), 6.98-7.10 (m, 4H),7.37 (d, J=8.1 Hz, 2H), 7.65 (d, J=8.1 Hz, 2H)

Example 50(53)N,N-bis(1H-imidazol-2-ylmethyl)-4-{[7-(2-phenylethyl)-2,7-diazaspiro[4.5]dec-2-yl]methyl}benzamide(Compound 50-53)

Description: amorphous;

TLC: Rf 0.51 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.28-1.81 (m, 6H), 2.24 (d, J=9.3 Hz, 2H), 2.28-2.68 (m,8H), 2.70-2.85 (m, 2H), 3.59 (s, 2H), 4.53-4.75 (m, 4H), 6.90-7.11 (m,4H), 7.13-7.31 (m, 5H), 7.38 (d, J=8.1 Hz, 2H), 7.61 (d, J=8.1 Hz, 2H)

Example 50(54)N,N-bis(1H-imidazol-2-ylmethyl)-4-{[7-(2-piperidine-1-ylethyl)-2,7-diazaspiro[4.5]dec-2-yl]methyl}benzamide(Compound 50-54)

Description: amorphous;

TLC: Rf 0.24 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.15-1.77 (m, 12H), 2.06-2.76 (m, 16H), 3.58 (q, J=13.2Hz, 2H), 4.47-4.82 (m, 4H), 6.87-7.14 (m, 4H), 7.36 (d, J=8.1 Hz, 2H),7.58 (d, J=8.1 Hz, 2H)

Example 50(55)4-{[8-(2,3-dihydro-1H-inden-2-yl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-55)

Description: amorphous;

TLC: Rf 0.37 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.58-1.71 (m, 6H), 2.35-2.39 (m, 3H), 2.42-2.51 (m, 3H),2.53-2.59 (m, 2H), 2.84-2.98 (m, 2H), 3.01-3.17 (m, 3H), 3.59 (s, 2H),4.61-4.74 (m, 4H), 6.94-7.07 (m, 4H), 7.09-7.19 (m, 4H), 7.30-7.41 (m,2H), 7.51-7.62 (m, 2H)

Example 50(56)4-[(8-cyclobutyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-56)

Description: amorphous;

TLC: Rf 0.45 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.56-1.71 (m, 8H), 1.85-1.93 (m, 2H), 1.95-2.05 (m, 3H),2.19-2.35 (m, 5H), 2.52-2.59 (m, 2H), 2.62-2.69 (m, 1H), 3.58 (s, 2H),4.59-4.72 (m, 4H), 6.95-7.10 (m, 4H), 7.31-7.40 (m, 2H), 7.56-7.64 (m,2H)

Example 50(57)N,N-bis(1H-imidazol-2-ylmethyl)-4-{[8-(1-propylpiperidin-4-yl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}benzamide(Compound 50-57)

Description: amorphous;

TLC: Rf 0.21 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 0.83-0.96 (m, 3H), 1.50-1.65 (m, 10H), 1.71-1.82 (m, 2H),1.85-1.96 (m, 2H), 2.22-2.37 (m, 5H), 2.42-2.58 (m, 6H), 2.93-3.06 (m,2H), 3.57 (s, 2H), 4.58-4.72 (m, 4H), 6.95-7.10 (m, 4H), 7.30-7.42 (m,2H), 7.55-7.68 (m, 2H)

Example 50(58)4-{[8-(2-adamantyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-58)

Description: amorphous;

TLC: Rf 0.50 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.32-1.43 (m, 2H), 1.55-1.70 (m, 10H), 1.74-1.87 (m, 4H),1.98-2.11 (m, 6H), 2.27-2.43 (m, 5H), 2.51-2.65 (m, 2H), 3.60 (s, 2H),4.59-4.73 (m, 4H), 6.94-7.08 (m, 4H), 7.30-7.41 (m, 2H), 7.55-7.66 (m,2H)

Example 50(59)N,N-bis(1H-imidazol-2-ylmethyl)-4-[(8-isobutyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]benzamide(Compound 50-59)

Description: amorphous;

TLC: Rf 0.48 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 0.80-0.94 (m, 6H), 1.51-1.65 (m, 6H), 1.68-1.83 (m, 1H),1.97-2.11 (m, 2H), 2.22-2.37 (m, 6H), 2.47-2.61 (m, 2H), 3.58 (s, 2H),4.57-4.72 (m, 4H), 6.95-7.10 (m, 4H), 7.30-7.45 (m, 2H), 7.51-7.66 (m,2H)

Example 50(60)4-(2,8-diazaspiro[4.5]dec-8-ylmethyl)-N,N-bis(1H-imidazol-2-ylmethyl)-2-methylbenzamide(Compound 50-60)

Description: amorphous;

TLC: Rf 0.38 (chloroform:methanol: 28% ammonia water=80:20:4);

NMR (DMSO-D₆): δ 1.36-1.51 (m, 6H), 2.11 (s, 3H), 2.21-2.34 (m, 4H),2.51-2.56 (m, 2H), 2.77 (t, J=7.2 Hz, 2H), 3.16 (s, 2H), 4.40 (s, 2H),4.68 (s, 2H), 6.79-7.02 (m, 4H), 7.02-7.08 (m, 1H), 7.08-7.19 (m, 2H),11.47-13.11 (m, 2H)

Example 50(61)4-[(2-cyclohexyl-2,8-diazaspiro[4.5]dec-8-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)-2-methylbenzamide(Compound 50-61)

Description: amorphous;

TLC: Rf 0.30 (chloroform:methanol: 28% ammonia water=80:10:1);

NMR (DMSO-D₆): δ 1.04-1.28 (m, 6H), 1.36-1.56 (m, 8H), 1.58-1.71 (m,2H), 1.71-1.85 (m, 2H), 1.88-1.97 (m, 1H), 2.11 (s, 3H), 2.17-2.31 (m,4H), 2.31-2.39 (m, 2H), 3.34 (s, 2H), 4.39 (s, 2H), 4.67 (s, 2H),6.64-7.00 (m, 4H), 7.00-7.07 (m, 1H), 7.07-7.18 (m, 2H), 12.05-12.42 (m,2H)

Example 50(62)4-{[(3-azepan-1-yl-2,2-dimethylpropyl)amino]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-62)

Description: amorphous;

TLC: Rf 0.52 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 0.86 (s, 6H), 1.48-1.62 (m, 8H), 2.36 (s, 2H), 2.44 (s,2H), 2.67-2.70 (m, 4H), 3.79 (s, 2H), 4.61 (br-s, 2H), 4.66 (br-s, 2H),7.00 (br-s, 2H), 7.07 (br-s, 2H), 7.38 (d, J=8.1 Hz, 2H), 7.62 (d, J=8.1Hz, 2H)

Example 50(63)N,N-bis(1H-imidazol-2-ylmethyl)-4-[({trans-4-[methyl(propyl)amino]cyclohexyl}amino)methyl]benzamide(Compound 50-63)

Description: amorphous;

TLC: Rf 0.44 (dichloromethane:methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 0.87 (t, J=7.2 Hz, 3H), 1.07-1.33 (m, 4H), 1.41-1.53 (m,2H), 1.82-1.86 (m, 2H), 1.99-2.02 (m, 2H), 2.25 (s, 3H), 2.36-2.41 (m,4H), 3.81 (s, 2H), 4.59 (br-s, 2H), 4.65 (br-s, 2H), 6.99 (br-s, 2H),7.06 (br-s, 2H), 7.35 (d, J=8.4 Hz, 2H), 7.61 (d, J=8.4 Hz, 2H)

Example 50(64)N,N-bis(1H-imidazol-2-ylmethyl)-4-[({trans-4-[propyl(tetrahydro-2H-pyran-4-yl)amino]cyclohexyl}amino)methyl]benzamide(Compound 50-64)

Description: amorphous;

TLC: Rf 0.46 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 0.81 (t, J=7.2 Hz, 3H), 1.06-1.18 (m, 2H), 1.25-1.42 (m,4H), 1.58-1.73 (m, 6H), 1.95-1.99 (m, 2H), 2.35-2.44 (m, 3H), 2.58 (m,1H), 2.73 (m, 1H), 3.35 (dt, J=11.0, 3.0 Hz, 2H), 3.81 (s, 2H),3.96-3.99 (m, 2H), 4.59 (br-s, 2H), 4.64 (br-s, 2H), 6.99 (br-s, 2H),7.06 (br-s, 2H), 7.35 (d, J=8.1 Hz, 2H), 7.62 (d, J=8.1 Hz, 2H)

Example 50(65)4-({[trans-4-(benzylamino)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-65)

Description: amorphous;

TLC: Rf 0.54 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 1.13-1.20 (m, 4H), 1.97-2.02 (m, 4H), 2.40-2.60 (m, 2H),3.81 (s, 2H), 3.82 (s, 2H), 4.57 (br-s, 2H), 4.62 (br-s, 2H), 7.00(br-s, 2H), 7.06 (br-s, 2H), 7.24-7.32 (m, 5H), 7.36 (d, J=8.1 Hz, 2H),7.62 (d, J=8.1 Hz, 2H)

Example 50(66)N,N-bis(1H-imidazol-2-ylmethyl)-4-({[trans-4-(propylamino)cyclohexyl]amino}methyl)benzamide(Compound 50-66)

Description: oily product;

TLC: Rf 0.27 (dichloromethane:methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 0.91 (t, J=7.2 Hz, 3H), 1.04-1.23 (m, 4H), 1.45-1.96 (m,6H), 2.40-2.50 (m, 2H), 2.58 (t, J=7.2 Hz, 2H), 3.82 (s, 2H), 4.55(br-s, 2H), 4.59 (br-s, 2H), 7.01 (br-s, 2H), 7.08 (br-s, 2H), 7.37 (d,J=8.1 Hz, 2H), 7.72 (d, J=8.1 Hz, 2H)

Example 50(67)4-{[{trans-4-[cyclohexyl(propyl)amino]cyclohexyl}(methyl)amino]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-67)

Description: oily product;

TLC: Rf 0.65 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 0.81 (t, J=7.2 Hz, 3H), 1.07-2.51 (m, 25H), 2.26 (s, 3H),3.55 (s, 2H), 4.63 (br-s, 2H), 4.65 (br-s, 2H), 6.98 (br-s, 2H), 7.06(br-s, 2H), 7.36 (d, J=8.1 Hz, 2H), 7.61 (d, J=8.1 Hz, 2H)

Example 50(68)4-[(benzyl{trans-4-[cyclohexyl(propyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-68)

Description: oily product;

TLC: Rf 0.83 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 0.81 (t, J=7.2 Hz, 3H), 1.22-2.60 (m, 25H), 3.60 (s, 2H),3.61 (s, 2H), 4.59 (br-s, 4H), 7.01 (br-s, 2H), 7.09 (br-s, 2H),7.18-7.35 (m, 5H), 7.42 (d, J=8.1 Hz, 2H), 7.67 (d, J=8.1 Hz, 2H)

Example 50(69)4-({[trans-4-(dipropylamino)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)-2-methylbenzamide(Compound 50-69)

Description: amorphous;

TLC: Rf 0.60 (chloroform:methanol: 28% ammonia water=80:20:4);

NMR (DMSO-D₆): δ 0.79 (t, J=7.50 Hz, 6H), 0.90-1.06 (m, 2H), 1.07-1.23(m, 2H), 1.23-1.39 (m, 4H), 1.56-1.72 (m, 2H), 1.83-1.98 (m, 2H), 2.11(s, 3H), 2.17-2.42 (m, 6H), 3.63 (s, 2H), 4.39 (s, 2H), 4.67 (s, 2H),6.72-7.05 (m, 4H), 7.05-7.20 (m, 3H), 11.97-12.55 (m, 2H)

Example 50(70)4-[(2-tert-butyl-2,8-diazaspiro[4.5]dec-8-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-70)

Description: amorphous;

TLC: Rf 0.52 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 1.07 (s, 9H), 1.52-1.66 (m, 6H), 2.24-2.44 (m, 4H), 2.54(s, 2H), 2.71 (t, J=6.6 Hz, 2H), 3.48 (s, 2H), 4.58-4.66 (m, 4H),6.98-7.10 (m, 4H), 7.37 (d, J=8.4 Hz, 2H), 7.65 (d, J=8.4 Hz, 2H)

Example 50(71)4-({[4-(tert-butylamino)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-71)

Description: amorphous;

TLC: Rf 0.26 (ethyl acetate: methanol: 28% ammonia water=90:10:2);

NMR (CDCl₃): δ d 1.12 (s, 9H), 1.48-1.75 (m, 8H), 2.62-2.78 (m, 2H),3.76 (s, 2H), 4.50-4.75 (m, 4H), 6.95-7.12 (m, 4H), 7.36 (d, J=8.4 Hz,2H), 7.60 (d, J=8.4 Hz, 2H)

Example 50(72)4-({[4-(tert-butylamino)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-72)

Description: amorphous;

TLC: Rf 0.19 (ethyl acetate: methanol: 28% ammonia water=90:10:2);

NMR (CDCl₃): δ d 1.12 (s, 9H), 1.10-1.30 (m, 4H), 1.75-2.00 (m, 4H),2.42 (m, 1H), 2.54 (m, 1H), 3.81 (s, 2H), 4.50-4.72 (m, 4H), 6.90-7.12(m, 4H), 7.35 (d, J=8.4 Hz, 2H), 7.63 (d, J=8.4 Hz, 2H)

Example 50(73)4-[({trans-4-[(cyclohexylmethyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-73)

Description: amorphous;

TLC: Rf 0.67 (ethyl acetate: methanol: 28% ammonia water=80:20:2);

NMR (CDCl₃): δ d 0.80-2.04 (m, 19H), 2.38-2.54 (m, 4H), 3.81 (s, 2H),4.50-4.68 (m, 4H), 6.92-7.12 (m, 4H), 7.35 (d, J=8.4 Hz, 2H), 7.64 (d,J=8.4 Hz, 2H)

Example 50(74)4-{[(4-anilinocyclohexyl)amino]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Low Polar Compound) (Compound 50-74)

Description: amorphous;

TLC: Rf 0.47 (ethyl acetate: methanol: 28% ammonia water=90:10:2);

NMR (CDCl₃): δ d 1.50-1.85 (m, 8H), 2.70 (m, 1H), 3.49 (m, 1H), 3.82 (s,2H), 4.50-4.68 (m, 4H), 6.59 (d, J=7.5 Hz, 2H), 6.66 (dd, J=7.5, 7.5 Hz,1H), 6.98-7.10 (m, 4H), 7.15 (dd, J=8.7, 7.5 Hz, 2H), 7.39 (d, J=8.4 Hz,2H), 7.68 (d, J=8.4 Hz, 2H)

Example 50(75)4-{[(4-anilinocyclohexyl)amino]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(High Polar Compound) (Compound 50-75)

Description: amorphous;

TLC: Rf 0.45 (ethyl acetate: methanol: 28% ammonia water=90:10:2);

NMR (CDCl₃): δ d 1.04-1.38 (m, 4H), 1.98-2.08 (m, 2H), 2.12-2.22 (m,2H), 2.52 (m, 1H), 3.24 (m, 1H), 3.85 (s, 2H), 4.46-4.70 (m, 4H), 6.58(d, J=7.5 Hz, 2H), 6.66 (dd, J=7.5, 7.5 Hz, 1H), 6.98-7.10 (m, 4H), 7.15(dd, J=8.4, 7.5 Hz, 2H), 7.39 (d, J=8.1 Hz, 2H), 7.70 (d, J=8.1 Hz, 2H)

Example 50(76)4-{[8-(2-cyclohexylethyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-76)

Description: amorphous;

TLC: Rf 0.28 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 0.83-0.97 (m, 2H), 1.13-1.27 (m, 4H), 1.36-1.45 (m, 2H),1.58-1.72 (m, 10H), 2.32-2.48 (m, 9H), 2.52-2.61 (m, 2H), 3.59 (s, 2H),4.58-4.70 (m, 4H), 6.98-7.12 (m, 4H), 7.32-7.43 (m, 2H), 7.62-7.74 (m,2H)

Example 50(77)4-{[8-(3-cyclohexylpropyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-77)

Description: amorphous;

TLC: Rf 0.28 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 0.78-0.92 (m, 2H), 1.10-1.26 (m, 6H), 1.53-1.57 (m, 2H),1.59-1.71 (m, 11H), 2.31-2.41 (m, 6H), 2.44-2.52 (m, 2H), 2.55-2.60 (m,2H), 3.59 (s, 2H), 4.56-4.68 (m, 4H), 6.98-7.12 (m, 4H), 7.33-7.42 (m,2H), 7.63-7.73 (m, 2H)

Example 50(78)N,N-bis(1H-imidazol-2-ylmethyl)-4-({8-[(3-methyl-2-thienyl)methyl]-2,8-diazaspiro[4.5]dec-2-yl}methyl)benzamide(Compound 50-78)

Description: amorphous;

TLC: Rf 0.40 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.52-1.65 (m, 6H), 2.15-2.21 (m, 3H), 2.30-2.45 (m, 6H),2.49-2.59 (m, 2H), 3.52-3.63 (m, 4H), 4.57-4.69 (m, 4H), 6.73-6.80 (m,1H), 6.97-7.12 (m, 5H), 7.32-7.43 (m, 2H), 7.60-7.69 (m, 2H)

Example 50(79)4-{[8-(1-benzothien-2-ylmethyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-79)

Description: amorphous;

TLC: Rf 0.40 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.54-1.67 (m, 6H), 2.34-2.49 (m, 6H), 2.51-2.60 (m, 2H),3.59 (s, 2H), 3.74 (s, 2H), 4.57-4.69 (m, 4H), 6.97-7.12 (m, 5H),7.22-7.34 (m, 2H), 7.35-7.41 (m, 2H), 7.61-7.69 (m, 3H), 7.72-7.80 (m,1H)

Example 50(80)4-{[8-(4-hydroxycyclohexyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-80)

Description: amorphous;

TLC: Rf 0.23 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.22-1.38 (m, 2H), 1.51-2.05 (m, 12H), 2.26-2.58 (m, 9H),3.53-4.02 (m, 3H), 4.56-4.66 (m, 4H), 6.95-7.10 (m, 4H), 7.33-7.44 (m,2H), 7.64-7.74 (m, 2H)

Example 50(81)4-{[8-(1-ethylpropyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-81)

Description: amorphous;

TLC: Rf 0.40 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 0.82-0.94 (m, 6H), 1.21-1.37 (m, 3H), 1.49-1.64 (m, 8H),2.17-2.28 (m, 1H), 2.36 (s, 2H), 2.45-2.53 (m, 3H), 2.55-2.61 (m, 2H),3.54-3.65 (m, 2H), 4.58-4.73 (m, 4H), 6.95-7.09 (m, 4H), 7.32-7.41 (m,2H), 7.58-7.68 (m, 2H)

Example 50(82)4-[(8-ethyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-82)

Description: amorphous;

TLC: Rf 0.16 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.02-1.12 (m, 3H), 1.54-1.66 (m, 6H), 2.27-2.42 (m, 8H),2.46-2.60 (m, 2H), 3.58 (s, 2H), 4.57-4.70 (m, 4H), 6.95-7.10 (m, 4H),7.32-7.41 (m, 2H), 7.54-7.65 (m, 2H)

Example 50(83)4-{[8-(1-azabicyclo[2.2.2]octa-3-yl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-83)

Description: amorphous;

TLC: Rf 0.25 (methanol: 28% ammonia water=9:1);

NMR (CDCl₃): δ 1.23-2.11 (m, 11H), 2.12-2.41 (m, 6H), 2.55 (t, J=6.9 Hz,2H), 2.63-3.15 (m, 7H), 3.58 (s, 2H), 4.54-4.72 (m, 4H), 6.92-7.15 (m,4H), 7.37 (d, J=8.1 Hz, 2H), 7.64 (d, J=8.1 Hz, 2H)

Example 50(84)4-{[2-(cyclopentylmethyl)-2,8-diazaspiro[4.5]dec-8-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-84)

Description: amorphous;

TLC: Rf 0.47 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.06-1.30 (m, 2H), 1.41-1.67 (m, 10H), 1.66-1.85 (m, 2H),1.88-2.08 (m, 1H), 2.25-2.44 (m, 8H), 2.55 (t, J=6.6 Hz, 2H), 3.47 (s,2H), 4.52-4.73 (m, 4H), 6.89-7.17 (m, 4H), 7.38 (d, J=8.1 Hz, 2H), 7.66(d, J=8.1 Hz, 2H)

Example 50(85)N,N-bis(1H-imidazol-2-ylmethyl)-4-{[2-(2-thienylmethyl)-2,8-diazaspiro[4.5]dec-8-yl]methyl}benzamide(Compound 50-85)

Description: amorphous;

TLC: Rf 0.58 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.49-1.70 (m, 6H), 2.20-2.38 (m, 4H), 2.41 (s, 2H), 2.62(t, J=6.9 Hz, 2H), 3.46 (s, 2H), 3.78 (s, 2H), 4.51-4.73 (m, 4H), 6.89(dd, J=3.6, 1.2 Hz, 1H), 6.93 (dd, J=4.8, 3.6 Hz, 1H), 6.96-7.13 (m,4H), 7.20 (dd, J=4.8, 1.2 Hz, 1H), 7.37 (d, J=8.1 Hz, 2H), 7.65 (d,J=8.1 Hz, 2H)

Example 50(86)4-{[2-(4-fluorobenzyl)-2,8-diazaspiro[4.5]dec-8-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-86)

Description: amorphous;

TLC: Rf 0.53 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.47-1.68 (m, 6H), 2.23-2.42 (m, 6H), 2.54 (t, J=6.9 Hz,2H), 3.46 (s, 2H), 3.53 (s, 2H), 4.52-4.72 (m, 4H), 6.86-7.15 (m, 6H),7.19-7.32 (m, 2H), 7.37 (d, J=8.1 Hz, 2H), 7.65 (d, J=8.1 Hz, 2H)

Example 50(87)4-({2-[4-(diethylamino)benzyl]-2,8-diazaspiro[4.5]dec-8-yl}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-87)

Description: amorphous;

TLC: Rf 0.44 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.15 (t, J=7.2 Hz, 6H), 1.45-1.68 (m, 6H), 2.24-2.43 (m,6H), 2.55 (t, J=6.6 Hz, 2H), 3.33 (q, J=7.2 Hz, 4H), 3.45 (s, 2H), 3.47(s, 2H), 4.51-4.73 (m, 4H), 6.62 (d, J=9.0 Hz, 2H), 6.92-7.09 (m, 4H),7.13 (d, J=9.0 Hz, 2H), 7.36 (d, J=8.1 Hz, 2H), 7.62 (d, J=8.1 Hz, 2H)

Example 50(88)4-{[4-(cyclopentylmethyl)-1-oxa-4,9-diazaspiro[5.5]undec-9-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-88)

Description: amorphous;

TLC: Rf 0.71 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.08-1.30 (m, 2H), 1.40-1.79 (m, 8H), 1.84-2.25 (m, 7H),2.27-2.56 (m, 6H), 3.52 (s, 2H), 3.62-3.75 (m, 2H), 4.50-4.75 (m, 4H),6.89-7.18 (m, 4H), 7.39 (d, J=8.1 Hz, 2H), 7.66 (d, J=8.1 Hz, 2H)

Example 50(89)N,N-bis(1H-imidazol-2-ylmethyl)-4-{[4-(2-thienylmethyl)-1-oxa-4,9-diazaspiro[5.5]undec-9-yl]methyl}benzamide(Compound 50-89)

Description: amorphous;

TLC: Rf 0.73 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.43-1.65 (m, 2H), 1.82-2.03 (m, 2H), 2.24 (s, 2H),2.28-2.57 (m, 6H), 3.49 (s, 2H), 3.63 (s, 2H), 3.67-3.80 (m, 2H),4.50-4.74 (m, 4H), 6.88 (dd, J=3.6, 1.2 Hz, 1H), 6.92 (dd, J=5.1, 3.6Hz, 1H), 6.95-7.12 (m, 4H), 7.21 (dd, J=5.1, 1.2 Hz, 1H), 7.37 (d, J=8.1Hz, 2H), 7.63 (d, J=8.1 Hz, 2H)

Example 50(90)4-{[{trans-4-[cyclohexyl(propyl)amino]cyclohexyl}(ethyl)amino]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-90)

Description: amorphous;

TLC: Rf 0.69 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 0.81 (t, J=7.2 Hz, 3H), 0.93 (t, J=7.2 Hz, 3H), 1.18-1.81(m, 20H), 2.39-2.53 (m, 7H), 3.59 (s, 2H), 4.64 (br-s, 4H), 6.99 (br-s,2H), 7.07 (br-s, 2H), 7.40 (d, J=8.1 Hz, 2H), 7.62 (d, J=8.1 Hz, 2H)

Example 50(91)4-{[{trans-4-[cyclohexyl(propyl)amino]cyclohexyl}(propyl)amino]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-91)

Description: oily product;

TLC: Rf 0.73 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 0.78-0.85 (m, 6H), 1.06-1.92 (m, 22H), 2.36-2.59 (m, 7H),3.60 (s, 2H), 4.62 (br-s, 4H), 7.01 (br-s, 2H), 7.09 (br-s, 2H), 7.41(d, J=8.1 Hz, 2H), 7.68 (d, J=8.1 Hz, 2H)

Example 50(92)4-[(2-cyclohexyl-2,8-diazaspiro[4.5]dec-8-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-92)

Description: amorphous;

TLC: Rf 0.15 (methanol: 28% ammonia water=99:1);

NMR (CDCl₃): δ 1.08-1.37 (m, 5H), 1.46-2.05 (m, 12H), 2.21-2.45 (m, 4H),2.56 (s, 2H), 2.74 (t, J=6.6 Hz, 2H), 3.47 (s, 2H), 4.50-4.75 (m, 4H),6.92-7.11 (m, 4H), 7.36 (d, J=8.1 Hz, 2H), 7.61 (d, J=8.1 Hz, 2H)

Example 50(93)4-[(8-cyclohexyl-2,8-diazaspiro[5.5]undec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-93)

Description: amorphous;

TLC: Rf 0.63 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 0.97-1.31 (m, 7H), 1.34-1.65 (m, 7H), 1.65-1.86 (m, 4H),2.01-2.63 (m, 9H), 3.32-3.51 (m, 2H), 4.52-4.85 (m, 4H), 6.88-7.12 (m,4H), 7.34 (d, J=8.1 Hz, 2H), 7.44 (d, J=8.1 Hz, 2H)

Example 50(94)4-[(4-cyclohexyl-1-oxa-4,9-diazaspiro[5.5]undec-9-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-94)

Description: amorphous;

TLC: Rf 0.42 (methanol: 28% ammonia water=99:1);

NMR (CDCl₃): δ 1.00-2.62 (m, 23H), 3.52 (s, 2H), 3.62-3.75 (m, 2H),4.49-4.75 (m, 4H), 6.94-7.14 (m, 4H), 7.39 (d, J=7.8 Hz, 2H), 7.63 (d,J=7.8 Hz, 2H)

Example 50(95)4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-95)

Description: amorphous;

TLC: Rf 0.33 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 1.00-2.56 (m, 25H), 3.56 (s, 2H), 4.64 (s, 2H), 4.68 (s,2H), 6.96 (s, 2H), 7.03 (s, 2H), 7.34 (d, J=8.1 Hz, 2H), 7.53 (d, J=8.1Hz, 2H)

Example 50(96)4-[(7-cyclohexyl-2,7-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-96)

Description: amorphous;

TLC: Rf 0.46 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 1.00-2.65 (m, 25H), 3.56 (d, J=13.8 Hz, 1H), 3.59 (d,J=13.8 Hz, 1H), 4.62-4.66 (m, 4H), 6.99 (s, 2H), 7.06 (s, 2H), 7.38 (d,J=8.1 Hz, 2H), 7.62 (d, J=8.1 Hz, 2H)

Example 50(97)4-[(9-cyclohexyl-2,9-diazaspiro[5.5]undec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-97)

Description: amorphous;

TLC: Rf 0.44 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 1.00-2.55 (m, 27H), 3.43 (s, 2H), 4.60-4.65 (m, 4H), 7.00(s, 2H), 7.07 (s, 2H), 7.37 (d, J=8.1 Hz, 2H), 7.40 (d, J=8.1 Hz, 2H)

Example 50(98)4-[(2-cyclohexyl-2,7-diazaspiro[3.5]nona-7-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-98)

Description: amorphous;

TLC: Rf 0.42 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 0.89-1.31 (m, 5H), 1.51-1.65 (m, 1H), 1.65-1.82 (m, 8H),1.95-2.11 (m, 1H), 2.16-2.41 (m, 4H), 3.06 (s, 4H), 3.43 (s, 2H),4.54-4.81 (m, 4H), 6.89-7.11 (m, 4H), 7.32 (d, J=8.1 Hz, 2H), 7.48 (d,J=8.1 Hz, 2H)

Example 50(99)4-[(9-cyclohexyl-1-oxa-4,9-diazaspiro[5.5]undec-4-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-99)

Description: amorphous;

TLC: Rf 0.51 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 0.96-1.36 (m, 5H), 1.44-1.69 (m, 3H), 1.70-2.09 (m, 6H),2.18 (s, 2H), 2.24-2.46 (m, 3H), 2.48-2.70 (m, 4H), 3.34-3.47 (m, 2H),3.62-3.78 (m, 2H), 4.53-4.83 (m, 4H), 6.90-7.12 (m, 4H), 7.33 (d, J=8.1Hz, 2H), 7.47 (d, J=8.1 Hz, 2H)

Example 50(100)4-[(4-cyclohexyl-1-oxa-4,8-diazaspiro[5.5]undec-8-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-100)

Description: amorphous;

TLC: Rf 0.72 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 0.98-1.33 (m, 5H), 1.37-1.90 (m, 9H), 2.04-2.69 (m, 9H),3.48 (d, J=13.8 Hz, 1H), 3.54 (d, J=13.8 Hz, 1H), 3.59-3.78 (m, 2H),4.53-4.84 (m, 4H), 6.89-7.13 (m, 4H), 7.34 (d, J=8.1 Hz, 2H), 7.48 (d,J=8.1 Hz, 2H)

Example 50(101)4-[(1′-cyclohexyl-1H-spiro[isoquinoline-4,4′-piperidine]-2(3H)-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-101)

Description: amorphous;

TLC: Rf 0.62 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 0.89-1.35 (m, 5H), 1.48-1.93 (m, 7H), 1.95-2.37 (m, 5H),2.62 (s, 2H), 2.67-2.88 (m, 2H), 3.60 (s, 2H), 3.66 (s, 2H), 4.48-4.87(m, 4H), 6.95 (d, J=7.87 Hz, 1H), 6.98-7.13 (m, 5H), 7.13-7.24 (m, 1H),7.42 (d, J=8.1 Hz, 2H), 7.47 (d, J=8.4 Hz, 1H), 7.53 (d, J=8.1 Hz, 2H)

Example 50(102)4-[({3-[(dipropylamino)methyl]phenyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-102)

Description: amorphous;

TLC: Rf 0.65 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 0.83 (t, J=7.2 Hz, 6H), 1.39-1.51 (m, 4H), 2.33-2.38 (m,4H), 3.46 (s, 2H), 4.35 (s, 2H), 4.60 (br-s, 2H), 4.67 (br-s, 2H), 6.47(m, 1H), 6.66-6.68 (m, 2H), 7.01 (br-s, 2H), 7.05 (br-s, 2H), 7.08 (m,1H), 7.42 (d, J=8.4 Hz, 2H), 7.64 (d, J=8.4 Hz, 2H)

Example 50(103)4-{[{trans-4-[cyclohexyl(propyl)amino]cyclohexyl}(2-hydroxyethyl)amino]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-103)

Description: amorphous;

TLC: Rf 0.47 (dichloromethane:methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 0.81 (t, J=7.5 Hz, 3H), 1.18-2.00 (m, 20H), 2.38-2.58 (m,5H), 2.65 (t, J=5.3 Hz, 2H), 3.41 (t, J=5.4 Hz, 2H), 3.63 (s, 2H), 4.57(br-s, 2H), 4.69 (br-s, 2H), 7.02 (br-s, 2H), 7.05 (br-s, 2H), 7.34 (d,J=8.1 Hz, 2H), 7.61 (d, J=8.1 Hz, 2H)

Example 50(104)4-[({trans-4-[(2-hydroxyethyl)(propyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-104)

Description: oily product;

TLC: Rf 0.41 (dichloromethane:methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 0.86 (t, J=7.5 Hz, 3H), 1.06-2.04 (m, 10H), 2.35-2.52 (m,4H), 2.59 (t, J=5.4 Hz, 2H), 3.48 (t, J=5.4 Hz, 2H), 3.82 (s, 2H), 4.58(br-s, 2H), 4.61 (br-s, 2H), 7.02 (br-s, 2H), 7.09 (br-s, 2H), 7.37 (d,J=8.1 Hz, 2H), 7.71 (d, J=8.1 Hz, 2H)

Example 50(105)4-[({trans-4-[(1-ethylpropyl)(2-hydroxyethyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-105)

Description: amorphous;

TLC: Rf 0.50 (dichloromethane:methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 0.89 (t, J=7.2 Hz, 6H), 1.11-1.42 (m, 8H), 1.71-1.75 (m,2H), 1.98-2.01 (m, 2H), 2.26 (m, 1H), 2.41 (m, 1H), 2.54 (m, 1H), 2.68(t, J=5.1 Hz, 2H), 3.45 (t, J=5.1 Hz, 2H), 3.81 (s, 2H), 4.60 (br-s,2H), 4.66 (br-s, 2H), 6.99 (br-s, 2H), 7.04 (br-s, 2H), 7.34 (d, J=7.8Hz, 2H), 7.52 (d, J=7.8 Hz, 2H)

Example 50(106)4-({[trans-4-(dipropylamino)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)-2-methoxybenzamide(Compound 50-106)

Description: amorphous;

TLC: Rf 0.67 (chloroform:methanol: 28% ammonia water=80:20:4);

NMR (DMSO-D₆): δ 0.79 (t, J=7.2 Hz, 6H), 0.90-1.07 (m, 2H), 1.07-1.23(m, 2H), 1.24-1.40 (m, 4H), 1.57-1.71 (m, 2H), 1.85-1.98 (m, 2H),2.17-2.43 (m, 6H), 3.64 (s, 3H), 3.67 (s, 2H), 4.43 (s, 2H), 4.54-4.95(m, 2H), 6.61-7.27 (m, 7H), 11.95-12.74 (m, 2H).

Example 50(107)4-[({trans-4-[2,3-dihydro-1H-inden-2-yl(propyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-107)

Description: amorphous;

TLC: Rf 0.64 (ethyl acetate:methanol: 28% ammonia water=80:20:3);

NMR (CDCl₃): δ d 0.85 (t, J=7.2 Hz, 3H), 1.15-1.60 (m, 6H), 1.82-1.95(m, 2H), 2.02-2.12 (m, 2H), 2.40-2.58 (m, 3H), 2.70 (m, 1H), 2.90-3.02(m, 4H), 3.77 (m, 1H), 3.84 (s, 2H), 4.52-4.75 (m, 4H), 6.98-7.10 (m,4H), 7.10-7.20 (m, 4H), 7.38 (d, J=8.4 Hz, 2H), 7.60 (d, J=8.4 Hz, 2H)

Example 50(108)N,N-bis(1H-imidazol-2-ylmethyl)-4-[({trans-4-[(2-naphthylmethyl)(propyl)amino]cyclohexyl}amino)methyl]benzamide(Compound 50-108)

Description: amorphous;

TLC: Rf 0.68 (ethyl acetate: methanol: 28% ammonia water=80:20:3);

NMR (CDCl₃): δ d 0.81 (t, J=7.2 Hz, 3H), 1.10-1.50 (m, 6H), 1.82-1.98(m, 2H), 2.00-2.12 (m, 2H), 2.42-2.50 (m, 2H), 2.50-2.70 (m, 2H), 3.75(s, 2H), 3.84 (s, 2H), 4.45-4.70 (m, 4H), 6.98-7.10 (m, 4H), 7.35-7.85(m, 11H)

Example 50(109)4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)-2-methoxybenzamide(Compound 50-109)

Description: amorphous;

TLC: Rf 0.63 (chloroform:methanol: 28% ammonia water=80:20:4);

NMR (DMSO-D₆): δ 0.91-1.30 (m, 5H), 1.36-1.60 (m, 7H), 1.61-1.80 (m,4H), 2.09-2.23 (m, 1H), 2.26 (s, 2H), 2.33-2.47 (m, 6H), 3.49 (s, 2H),3.63 (s, 3H), 4.31-4.54 (m, 2H), 4.58-4.87 (m, 2H), 6.59-7.25 (m, 7H),12.04-12.76 (m, 2H)

Example 50(110)4-[({4-[(dipropylamino)methyl]benzyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-110)

Description: amorphous;

TLC: Rf 0.59 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 0.85 (t, J=7.5 Hz, 6H), 1.41-1.54 (m, 4H), 2.34-2.39 (m,4H), 3.54 (s, 2H), 3.77 (s, 2H), 3.82 (s, 2H), 4.61 (br-s, 2H), 4.66(br-s, 2H), 6.97 (br-s, 2H), 7.04 (br-s, 2H), 7.23-7.30 (m, 4H), 7.38(d, J=7.8 Hz, 2H), 7.58-7.61 (m, 2H)

Example 50(111)4-[({3-[(dipropylamino)methyl]benzyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-111)

Description: amorphous;

TLC: Rf 0.59 (ethyl acetate: methanol: 28% ammonia water=50:10:1);

NMR (CDCl₃): δ 0.85 (t, J=7.5 Hz, 6H), 1.42-1.54 (m, 4H), 2.35-2.40 (m,4H), 3.55 (s, 2H), 3.78 (s, 2H), 3.82 (s, 2H), 4.58 (br-s, 2H), 4.62(br-s, 2H), 7.01 (br-s, 2H), 7.08 (br-s, 2H), 7.17-7.29 (m, 4H), 7.41(d, J=8.1 Hz, 2H), 7.71 (d, J=8.1 Hz, 2H)

Example 50(112)3-[{4-[(4-{[bis(1H-imidazol-2-ylmethyl)amino]carbonyl}benzyl)amino]cyclohexyl}(propyl)amino]propanoicacid (High Polar Compound) (Compound 50-112)

Description: amorphous;

TLC: Rf 0.24 (dichloromethane:methanol: 28% ammonia water=80:20:2);

NMR (DMSO-D₆): δ d 0.81 (t, J=7.5 Hz, 3H), 0.95-1.12 (m, 2H), 1.15-1.32(m, 2H), 1.32-1.48 (m, 2H), 1.60-1.72 (m, 2H), 1.85-1.98 (m, 2H), 2.16(t, J=7.2 Hz, 2H), 2.25 (m, 1H), 2.43 (t, J=7.2 Hz, 2H), 2.56 (m, 1H),2.69 (t, J=6.6 Hz, 2H), 3.70 (s, 2H), 4.45-4.68 (m, 4H), 6.90-7.08 (m,4H), 7.32 (d, J=8.4 Hz, 2H), 7.42 (d, J=8.4 Hz, 2H)

Example 50(113)N,N-bis(1H-imidazol-2-ylmethyl)-4-{[8-(3-methyl-2-butene-1-yl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}benzamide(Compound 50-113)

Description: amorphous;

TLC: Rf 0.44 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.49-1.66 (m, 9H), 1.72 (s, 3H), 2.21-2.46 (m, 6H), 2.55(t, J=6.9 Hz, 2H), 2.92 (d, J=6.9 Hz, 2H), 3.57 (s, 2H), 4.52-4.73 (m,4H), 5.15-5.33 (m, 1H), 6.86-7.14 (m, 4H), 7.36 (d, J=8.1 Hz, 2H), 7.61(d, J=8.1 Hz, 2H)

Example 50(114)4-{[8-(2-ethylbutyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-114)

Description: amorphous;

TLC: Rf 0.58 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 0.83 (t, J=7.2 Hz, 6H), 1.15-1.46 (m, 5H), 1.48-1.68 (m,6H), 2.08 (d, J=6.6 Hz, 2H), 2.16-2.39 (m, 6H), 2.54 (t, J=6.6 Hz, 2H),3.57 (s, 2H), 4.50-4.77 (m, 4H), 6.82-7.16 (m, 4H), 7.36 (d, J=8.1 Hz,2H), 7.59 (d, J=8.1 Hz, 2H)

Example 50(115)N,N-bis(1H-imidazol-2-ylmethyl)-4-({8-[(5-methyl-2-thienyl)methyl]-2,8-diazaspiro[4.5]dec-2-yl}methyl)benzamide(Compound 50-115)

Description: amorphous;

TLC: Rf 0.41 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.52-1.64 (m, 6H), 2.31-2.45 (m, 9H), 2.49-2.59 (m, 2H),3.52-3.63 (m, 4H), 4.58-4.71 (m, 4H), 6.52-6.59 (m, 1H), 6.62-6.68 (m,1H), 6.95-7.09 (m, 4H), 7.31-7.40 (m, 2H), 7.56-7.65 (m, 2H)

Example 50(116)4-({8-[(5-chloro-2-thienyl)methyl]-2,8-diazaspiro[4.5]dec-2-yl}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-116)

Description: amorphous;

TLC: Rf 0.40 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.52-1.65 (m, 6H), 2.29-2.43 (m, 6H), 2.47-2.60 (m, 2H),3.51-3.61 (m, 4H), 4.58-4.71 (m, 4H), 6.60-6.66 (m, 1H), 6.67-6.74 (m,1H), 6.95-7.09 (m, 4H), 7.31-7.41 (m, 2H), 7.55-7.65 (m, 2H)

Example 50(117)N,N-bis(1H-imidazol-2-ylmethyl)-4-{[8-(1,3-thiazol-2-ylmethyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}benzamide(Compound 50-117)

Description: amorphous;

TLC: Rf 0.41 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.54-1.67 (m, 6H), 2.34 (s, 2H), 2.43-2.58 (m, 6H), 3.58(s, 2H), 3.79 (s, 2H), 4.58-4.70 (m, 4H), 6.94-7.08 (m, 4H), 7.22-7.28(m, 1H), 7.30-7.40 (m, 2H), 7.56-7.65 (m, 2H), 7.65-7.71 (m, 1H)

Example 50(118)N,N-bis(1H-imidazol-2-ylmethyl)-4-{[8-(1,3-oxazol-2-ylmethyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}benzamide(Compound 50-118)

Description: amorphous;

TLC: Rf 0.41 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.54-1.67 (m, 6H), 2.32 (s, 2H), 2.37-2.49 (m, 4H),2.49-2.57 (m, 2H), 3.57 (s, 2H), 3.65 (s, 2H), 4.57-4.69 (m, 4H),6.95-7.09 (m, 5H), 7.31-7.39 (m, 2H), 7.57-7.66 (m, 3H)

Example 50(119)3-chloro-4-({[trans-4-(dipropylamino)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-119)

Description: oily product;

TLC: Rf 0.49 (chloroform:methanol: 28% ammonia water=40:10:2);

NMR (METHANOL-D₄): δ 0.77-0.93 (m, 6H), 1.04-1.54 (m, 8H), 1.68-1.92 (m,2H), 1.96-2.14 (m, 2H), 2.26-2.59 (m, 6H), 3.83-3.93 (m, 2H), 4.31-4.87(m, 4H), 7.01 (s, 4H), 7.33-7.51 (m, 2H), 7.55 (s, 1H)

Example 50(120)3-chloro-4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-120)

Description: amorphous;

TLC: Rf 0.49 (chloroform:methanol: 28% ammonia water=40:10:2);

NMR (METHANOL-D₄): δ 1.01-1.35 (m, 6H), 1.46-1.70 (m, 6H), 1.72-1.83 (m,2H), 1.85-1.99 (m, 2H), 2.15-2.36 (m, 1H), 2.40-2.76 (m, 8H), 3.60-3.93(m, 2H), 4.33-4.85 (m, 4H), 6.80-7.22 (m, 4H), 7.34-7.46 (m, 1H),7.47-7.65 (m, 2H)

Example 50(121)4-[(8-cyclooctyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-121)

Description: amorphous;

TLC: Rf 0.22 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.40-1.75 (m, 18H), 2.31-2.60 (m, 11H), 3.59 (s, 2H),4.55-4.69 (m, 4H), 6.94-7.10 (m, 4H), 7.31-7.41 (m, 2H), 7.59-7.68 (m,2H)

Example 50(122)N,N-bis(1H-imidazol-2-ylmethyl)-4-[(8-methyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]benzamide(Compound 50-122)

Description: amorphous;

TLC: Rf 0.12 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.53-1.66 (m, 6H), 2.19-2.34 (m, 9H), 2.48-2.61 (m, 2H),3.60 (s, 2H), 4.59-4.71 (m, 4H), 6.95-7.10 (m, 4H), 7.30-7.42 (m, 2H),7.55-7.66 (m, 2H)

Example 50(123)4-[(8-acetyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-123)

Description: amorphous;

TLC: Rf 0.41 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.50-1.75 (m, 6H), 2.06 (s, 3H), 2.31-2.46 (m, 2H),2.53-2.68 (m, 2H), 3.25-3.64 (m, 6H), 4.60-4.74 (m, 4H), 6.92-7.07 (m,4H), 7.30-7.42 (m, 2H), 7.55-7.67 (m, 2H)

Example 50(124)[2-(4-{[bis(1H-imidazol-2-ylmethyl)amino]carbonyl}benzyl)-2,8-diazaspiro[4.5]dec-8-yl]aceticacid (Compound 50-124)

Description: amorphous;

TLC: Rf 0.53 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.79-1.93 (m, 6H), 2.63-2.71 (m, 2H), 2.79-2.88 (m, 2H),3.15-3.35 (m, 4H), 3.56 (s, 2H), 3.81 (s, 2H), 4.59-4.85 (m, 4H),7.00-7.09 (m, 4H), 7.39-7.51 (m, 4H)

Example 50(125)3-{[trans-4-(dipropylamino)cyclohexyl]amino}-N,N-bis(1H-imidazol-2-ylmethyl)propaneamide(Compound 50-125)

Description: amorphous;

TLC: Rf 0.57 (dichloromethane:methanol:ammonia water=40:10:1);

NMR (CDCl₃): δ 0.85 (t, J=7.2 Hz, 6H), 1.00-1.48 (m, 8H), 1.74-1.84 (m,2H), 1.90-2.00 (m, 2H), 2.30-2.50 (m, 6H), 2.72 (t, J=6.0 Hz, 2H), 2.92(t, J=6.0 Hz, 2H), 4.64 (s, 2H), 4.69 (s, 2H), 6.96 (s, 4H)

Example 50(126)3-(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)-N,N-bis(1H-imidazol-2-ylmethyl)propaneamide(Compound 50-126)

Description: amorphous;

TLC: Rf 0.61 (dichloromethane:methanol:ammonia water=40:10:1);

NMR (CDCl₃): δ 1.00-1.34 (m, 6H), 1.59-1.73 (m, 6H), 1.78-1.85 (m, 2H),1.88-1.97 (m, 2H), 2.48 (m, 1H), 2.56 (s, 2H), 2.58-2.70 (m, 4H), 2.77(t, J=6.9 Hz, 2H), 2.81 (t, J=6.6 Hz, 2H), 2.91 (t, J=6.9 Hz, 2H), 4.67(s, 2H), 4.71 (s, 2H), 6.99 (s, 4H)

Example 50(127)4-(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)-N,N-bis(1H-imidazol-2-ylmethyl)butaneamide(Compound 50-127)

Description: amorphous;

TLC: Rf 0.57 (dichloromethane:methanol:ammonia water=40:10:1);

NMR (CDCl₃): δ 1.03-1.44 (m, 8H), 1.63-1.73 (m, 2H), 1.79-2.10 (m, 10H),2.64 (t, J=6.9 Hz, 2H), 2.70 (s, 2H), 2.78-2.94 (m, 5H), 3.02 (t, J=6.9Hz, 2H), 4.72 (s, 2H), 4.76 (s, 2H), 6.99 (s, 2H), 7.00 (s, 2H)

Example 50(128)5-(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)-N,N-bis(1H-imidazol-2-ylmethyl)pentaneamide(Compound 50-128)

Description: amorphous;

TLC: Rf 0.60 (dichloromethane:methanol:ammonia water=40:10:1);

NMR (CDCl₃): δ 1.06-1.50 (m, 8H), 1.58-1.76 (m, 4H), 1.84-1.96 (m, 4H),2.00-2.20 (m, 4H), 2.47 (t, J=6.6 Hz, 2H), 2.80-3.18 (m, 11H), 4.80 (s,2H), 4.82 (s, 2H), 7.00 (s, 2H), 7.02 (s, 2H)

Example 50(129)4-[({4-[(4,4-difluorocyclohexyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Low Polar Compound) (Compound 50-129)

Description: amorphous;

TLC: Rf 0.38 (ethyl acetate: methanol: 28% ammonia water=80:20:3);

NMR (CDCl₃): δ d 1.40-2.20 (m, 16H), 2.65-2.80 (m, 3H), 3.79 (s, 2H),4.52-4.72 (m, 4H), 6.95-7.12 (m, 4H), 7.37 (d, J=8.1 Hz, 2H), 7.62 (d,J=8.1 Hz, 2H)

Example 50(130)4-[({4-[(4,4-difluorocyclohexyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(High Polar Compound) (Compound 50-130)

Description: amorphous;

TLC: Rf 0.28 (ethyl acetate: methanol: 28% ammonia water=80:20:3);

NMR (CDCl₃): δ d 1.00-2.20 (m, 16H), 2.40-3.00 (m, 3H), 3.84 (s, 2H),4.50-4.70 (m, 4H), 6.95-7.12 (m, 4H), 7.38 (d, J=8.1 Hz, 2H), 7.67 (d,J=8.1 Hz, 2H)

Example 50(131)(2E)-3-{4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]phenyl}-N,N-bis(1H-imidazol-2-ylmethyl)acrylamide(Compound 50-131)

Description: amorphous;

TLC: Rf 0.60 (ethyl acetate:methanol:ammonia water=50:10:1);

NMR (CDCl₃): δ 1.05-2.33 (m, 16H), 2.44-2.57 (m, 9H), 3.56 (s, 2H), 4.80(br-s, 2H), 4.90 (br-s, 2H), 6.99 (br-s, 2H), 7.01 (br-s, 2H), 7.08 (d,J=15.3 Hz, 1H), 7.29 (d, J=6.9 Hz, 2H), 7.45 (d, J=6.9 Hz, 2H), 7.61 (d,J=15.3 Hz, 1H)

Example 50(132)(2E)-3-[4-({[trans-4-(dipropylamino)cyclohexyl]amino}methyl)phenyl]-N,N-bis(1H-imidazol-2-ylmethyl)acrylamide(Compound 50-132)

Description: amorphous;

TLC: Rf 0.59 (ethyl acetate:methanol:ammonia water=50:10:1);

NMR (CDCl₃): δ 0.85 (t, J=7.5 Hz, 6H), 1.06-1.50 (m, 8H), 1.80-1.83 (m,2H), 1.98-2.02 (m, 2H), 2.37-2.51 (m, 6H), 3.79 (s, 2H), 4.79 (br-s,2H), 4.89 (br-s, 2H), 7.00 (br-s, 4H), 7.07 (d, J=15.3 Hz, 1H), 7.27 (d,J=8.4 Hz, 2H), 7.45 (d, J=8.4 Hz, 2H), 7.59 (d, J=15.3 Hz, 1H)

Example 50(133)3-{4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]phenyl}-N,N-bis(1H-imidazol-2-ylmethyl)propaneamide(Compound 50-133)

Description: amorphous;

TLC: Rf 0.55 (ethyl acetate:methanol:ammonia water=50:10:1);

NMR (CDCl₃): δ 1.05-1.27 (m, 6H), 1.57-1.63 (m, 8H), 1.70-1.80 (m, 2H),1.85-1.90 (m, 2H), 2.28 (m, 1H), 2.45-2.57 (m, 6H), 2.76-2.88 (m, 4H),3.49 (s, 2H), 4.48 (br-s, 2H), 4.56 (br-s, 2H), 6.97 (br-s, 2H), 6.98(br-s, 2H), 7.01 (d, J=8.1 Hz, 2H), 7.12 (d, J=8.1 Hz, 2H)

Example 50(134)3-[4-({[trans-4-(dipropylamino)cyclohexyl]amino}methyl)phenyl]-N,N-bis(1H-imidazol-2-ylmethyl)propaneamide(Compound 50-134)

Description: amorphous;

TLC: Rf 0.56 (ethyl acetate:methanol:ammonia water=50:10:1);

NMR (CDCl₃): δ 0.86 (t, J=7.5 Hz, 6H), 1.10-2.03 (m, 12H), 2.39-2.51 (m,6H), 2.76-2.85 (m, 4H), 3.74 (s, 2H), 4.46 (br-s, 2H), 4.52 (br-s, 2H),6.97 (br-s, 2H), 6.98 (br-s, 2H), 7.02 (d, J=8.1 Hz, 2H), 7.12 (d, J=8.1Hz, 2H)

Example 50(135)2-{4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]phenyl}-N,N-bis(1H-imidazol-2-ylmethyl)acetamide(Compound 50-135)

Description: amorphous;

TLC: Rf 0.59 (ethyl acetate:methanol:ammonia water=50:10:1);

NMR (CDCl₃): δ 1.10-1.30 (m, 6H), 1.59-2.02 (m, 14H), 2.44-2.63 (m, 5H),3.54 (s, 2H), 3.79 (s, 2H), 4.64 (br-s, 2H), 4.73 (br-s, 2H), 6.98(br-s, 2H), 6.99 (br-s, 2H), 7.13 (d, J=8.1 Hz, 2H), 7.23 (d, J=8.1 Hz,2H)

Example 50(136)2-[4-({[trans-4-(dipropylamino)cyclohexyl]amino}methyl)phenyl]-N,N-bis(1H-imidazol-2-ylmethyl)acetamide(Compound 50-136)

Description: amorphous;

TLC: Rf 0.57 (ethyl acetate:methanol:ammonia water=50:10:1);

NMR (CDCl₃): δ 0.86 (t, J=7.5 Hz, 6H), 1.09-1.49 (m, 8H), 1.81-1.85 (m,2H), 2.00-2.04 (m, 2H), 2.40-2.52 (m, 6H), 3.76 (s, 2H), 3.78 (s, 2H),4.61 (br-s, 2H), 4.72 (br-s, 2H), 6.97 (br-s, 2H), 7.00 (br-s, 2H), 7.11(d, J=8.1 Hz, 2H), 7.21 (d, J=8.1 Hz, 2H)

Example 50(137)4-{[8-(cyclobutylmethyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-137)

Description: amorphous;

TLC: Rf 0.34 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.57-1.90 (m, 10H), 1.96-2.11 (m, 3H), 2.31-2.46 (m, 7H),2.50-2.62 (m, 3H), 3.58 (s, 2H), 4.57-4.71 (m, 4H), 6.93-7.08 (m, 4H),7.29-7.39 (m, 2H), 7.54-7.64 (m, 2H)

Example 50(138)4-[({4-[(4,4-difluorocyclohexyl)(propyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Low Polar Compound) (Compound 50-138)

Description: amorphous;

TLC: Rf 0.73 (ethyl acetate: methanol: 28% ammonia water=80:20:3);

NMR (CDCl₃): δ 0.82 (t, J, 7.5 Hz, 3H), 1.30-1.84 (m, 16H), 2.00-2.20(m, 2H), 2.43 (t, J, 7.5 Hz, 2H), 2.54 (m, 1H), 2.72 (m, 1H), 2.76 (m,1H), 3.77 (s, 2H), 4.60-4.80 (m, 4H), 6.95-7.12 (m, 4H), 7.37 (d, J=8.4Hz, 2H), 7.51 (d, J=8.4 Hz, 2H)

Example 50(139)4-[({4-[(4,4-difluorocyclohexyl)(propyl)amino]cyclohexyl}amino)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(High Polar Compound) (Compound 50-139)

Description: amorphous;

TLC: Rf 0.60 (ethyl acetate: methanol: 28% ammonia water=80:20:3);

NMR (CDCl₃): δ d 0.82 (t, J=7.5 Hz, 3H), 1.02-1.90 (m, 14H), 1.92-2.20(m, 4H), 2.38 (m, 1H), 2.40 (t, J, 7.5 Hz, 2H), 2.52 (m, 1H), 2.64 (m,1H), 3.81 (s, 2H), 4.60-4.80 (m, 4H), 6.98-7.12 (m, 4H), 7.34 (d, J=8.1Hz, 2H), 7.52 (d, J=8.1 Hz, 2H)

Example 50(140)4-({[4-(1-azocanyl)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Low Polar Compound) (Compound 50-140)

Description: amorphous;

TLC: Rf 0.53 (ethyl acetate: methanol: 28% ammonia water=90:10:2);

NMR (CDCl₃): δ d 1.40-1.82 (m, 18H), 2.41 (m, 1H), 2.55-2.70 (m, 4H),2.73 (m, 1H), 3.78 (s, 2H), 4.60-4.80 (m, 4H), 6.98-7.12 (m, 4H), 7.37(d, J=8.1 Hz, 2H), 7.53 (d, J, 8.1 Hz, 2H)

Example 50(141)4-({[4-(1-azocanyl)cyclohexyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(High Polar Compound) (Compound 50-141)

Description: amorphous;

TLC: Rf 0.37 (ethyl acetate: methanol: 28% ammonia water=90:10:2);

NMR (CDCl₃): δ d 1.04-1.38 (m, 4H), 1.45-1.68 (m, 10H), 1.75-1.88 (m,2H), 1.92-2.08 (m, 2H), 2.32-2.48 (m, 2H), 2.50-2.68 (m, 4H), 3.81 (s,2H), 4.58-4.78 (m, 4H), 6.92-7.12 (m, 4H), 7.33 (d, J=8.1 Hz, 2H), 7.53(d, J=8.1 Hz, 2H)

Example 50(142)4-({[(2E)-4-(diethylamino)-2-butene-1-yl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-142)

Description: oily product;

TLC: Rf 0.26 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.02 (t, J=6.9 Hz, 3H), 1.10 (t, J=7.2 Hz, 3H), 2.48 (q,J=6.9 Hz, 2H), 2.64 (q, J=7.2 Hz, 2H), 3.03 (d, J=4.8 Hz, 2H), 3.22 (d,J=4.8 Hz, 2H), 3.55 (s, 2H), 4.64 (m, 4H), 5.64 (m, 2H), 7.01 (m, 4H),7.37 (d, J=8.1 Hz, 2H), 7.59 (d, J=8.1 Hz, 2H)

Example 50(143)N,N-bis(1H-imidazol-2-ylmethyl)-4-{[8-(2,2,6,6-tetramethyl-4-piperidinyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}benzamide(Compound 50-143)

Description: amorphous;

TLC: Rf 0.25 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.05 (t, J=12.3 Hz, 2H), 1.12 (s, 6H), 1.18 (s, 6H),1.51-1.66 (m, 6H), 1.74 (dd, J=12.3, 3.0 Hz, 2H), 2.33 (s, 2H),2.37-2.63 (m, 6H), 2.68-2.87 (m, 1H), 3.58 (s, 2H), 4.51-4.76 (m, 4H),6.86-7.16 (m, 4H), 7.38 (d, J=8.1 Hz, 2H), 7.64 (d, J=8.1 Hz, 2H)

Example 50(144)4-[(7-cycloheptyl-2,7-diazaspiro[3.5]nona-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-144)

Description: amorphous;

TLC: Rf 0.34 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.24-1.86 (m, 16H), 2.30-2.45 (m, 4H), 2.44-2.59 (m, 1H),2.99 (s, 4H), 3.63 (s, 2H), 4.49-4.73 (m, 4H), 6.88-7.15 (m, 4H), 7.32(d, J=8.1 Hz, 2H), 7.62 (d, J=8.1 Hz, 2H)

Example 50(145)4-[(5-cycloheptyl-2,5-diazabicyclo[2.2.1]hepta-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-145)

Description: amorphous;

TLC: Rf 0.38 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.19-1.88 (m, 14H), 2.42-2.62 (m, 3H), 2.82 (d, J=9.6 Hz,1H), 2.96 (dd, J=9.6, 2.7 Hz, 1H), 3.21 (s, 1H), 3.56 (s, 1H), 3.66 (d,J=13.9 Hz, 1H), 3.76 (d, J=13.90 Hz, 1H), 4.51-4.77 (m, 4H), 6.89-7.16(m, 4H), 7.41 (d, J=8.1 Hz, 2H), 7.64 (d, J=8.1 Hz, 2H)

Example 50(146)4-({[(1-cycloheptyl-4-piperidinyl)methyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzamide(Compound 50-146)

Description: amorphous;

TLC: Rf 0.30 (methanol: 28% ammonia water=98:2);

NMR (CDCl₃): δ 1.08-1.90 (m, 17H), 2.12-2.31 (m, 2H), 2.47 (d, J=6.9 Hz,2H), 2.50-2.61 (m, 1H), 2.69-2.87 (m, 2H), 3.77 (s, 2H), 4.46-4.76 (m,4H), 6.86-7.14 (m, 4H), 7.30-7.43 (m, 2H), 7.54-7.70 (m, 2H)

Example 50(147)3-[{4-[(4-{[bis(1H-imidazol-2-ylmethyl)amino]carbonyl}benzyl)amino]cyclohexyl}(propyl)amino]propanoicacid (Low Polar Compound) (Compound 50-147)

Description: amorphous;

TLC: Rf 0.47 (dichloromethane: methanol: 28% ammonia water=80:20:2);

NMR (DMSO-D₆): δ d 0.84 (t, J=7.5 Hz, 3H), 1.22-1.82 (m, 10H), 2.21 (t,J=6.6 Hz, 2H), 2.53 (m, 2H), 2.60-2.75 (m, 2H), 2.78 (t, J, 6.6 Hz, 2H),3.66 (s, 2H), 4.50-4.68 (m, 4H), 6.90-7.08 (m, 4H), 7.35 (d, J=8.1 Hz,2H), 7.43 (d, J=8.1 Hz, 2H)

Example 51(1) to Example 51(2)

The same procedure as a series of reactions of Example 16→Example17→Example 18→Example 19 was carried out, except that1-(1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-imidazol-2-yl)methaneaminewas used or a corresponding amine compound was used in place of it inthe process of Example 16, 1H-imidazole-2-carboxylic acid was used or acorresponding carboxylic acid was used in place of it in the process ofExample 17, and N,N-dipropyl-1,4-butanediamine was used or acorresponding amine was used in place of it in the process of Example19, to obtain the title compound having the following physicalproperties

Example 51(1)N-(1H-benzoimidazol-2-ylmethyl)-N-{4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]benzyl}-1H-imidazole-2-carboxamide(Compound 51-1)

Description: amorphous;

TLC: Rf 0.32 (methanol: 28% ammonia water=98:2);

NMR (DMSO-d₆): δ 1.06-1.19 (m, 5H), 1.40-1.56 (m, 7H), 1.61-1.74 (m,4H), 2.12-2.20 (m, 1H), 2.22-2.28 (m, 2H), 2.32-2.47 (m, 6H), 3.48 (s,2H), 4.61-4.74 (m, 2H), 5.57-5.69 (m, 2H), 7.11-7.26 (m, 8H), 7.43-7.55(m, 2H)

Example 51(2)N-(1H-benzoimidazol-2-ylmethyl)-N-{4-[({trans-4-[cyclohexyl(propyl)amino]cyclohexyl}amino)methyl]benzyl}-1H-imidazole-2-carboxamide(Compound 51-2)

Description: amorphous;

TLC: Rf 0.28 (methanol: 28% ammonia water=98:2);

NMR (DMSO-d₆): δ 0.73-0.83 (m, 3H), 0.97-1.10 (m, 4H), 1.13-1.29 (m,7H), 1.53-1.69 (m, 7H), 1.85-1.98 (m, 2H), 2.21-2.31 (m, 1H), 2.35-2.45(m, 4H), 3.67 (s, 2H), 4.62-4.73 (m, 2H), 5.56-5.69 (m, 2H), 7.09-7.17(m, 3H), 7.20-7.31 (m, 5H), 7.41-7.57 (m, 2H)

Example 52(1) to Example 52(4)

The same procedure as a series of reactions of Example 44→Example45→Example 46 was carried out, except that a corresponding carboxylicacid was used in place of [1-(tert-butoxycarbonyl)-4-piperidinyl]aceticacid, to obtain the title compound having the following physicalproperties.

Example 52(1)3-{1-[3-(dipropylamino)propyl]piperidin-4-yl}-N,N-bis(1H-imidazol-2-ylmethyl)propaneamide(Compound 52-1)

Description: amorphous;

TLC: Rf 0.84 (chloroform:methanol: 28% ammonia water=40:10:2);

NMR (METHANOL-D₄): δ 0.89 (t, J=7.2 Hz, 6H), 1.09-1.36 (m, 3H),1.36-1.55 (m, 6H), 1.57-1.71 (m, 4H), 1.83-1.98 (m, 2H), 2.25-2.34 (m,2H), 2.35-2.51 (m, 8H), 2.84-2.97 (m, 2H), 4.66 (s, 2H), 4.70 (s, 2H),6.96 (s, 2H), 7.02 (s, 2H)

Example 52(2)1-[6-(dipropylamino)hexyl]-N,N-bis(1H-imidazol-2-ylmethyl)piperidine-4-carboxamide(Compound 52-2)

Description: oily product;

TLC: Rf 0.73 (chloroform:methanol: 28% ammonia water=40:10:2);

NMR (METHANOL-D₄): δ 0.88 (t, J=7.5 Hz, 6H), 1.16-1.36 (m, 6H),1.39-1.59 (m, 8H), 1.60-1.84 (m, 2H), 1.86-2.06 (m, 2H), 2.22-2.33 (m,2H), 2.36-2.55 (m, 6H), 2.57-2.77 (m, 1H), 2.82-3.00 (m, 2H), 4.66 (s,2H), 4.75 (s, 2H), 6.97 (s, 2H), 7.03 (s, 2H)

Example 52(3)4-{[trans-4-(dipropylamino)cyclohexyl]amino}-N,N-bis(1H-imidazol-2-ylmethyl)butaneamide(Compound 52-3)

Description: amorphous;

TLC: Rf 0.39 (dichloromethane: methanol: 28% ammonia water=40:10:1);

NMR (CDCl₃): δ 0.88 (t, J=7.2 Hz, 6H), 1.32-1.68 (m, 8H), 1.90-2.04 (m,2H), 2.13-2.24 (m, 2H), 2.47-2.79 (m, 9H), 2.86-3.03 (m, 3H), 4.69 (s,2H), 4.72 (s, 2H), 6.94 (s, 2H), 6.97 (s, 2H)

Example 52(4)5-{[trans-4-(dipropylamino)cyclohexyl]amino}-N,N-bis(1H-imidazol-2-ylmethyl)pentaneamide(Compound 52-4)

Description: amorphous;

TLC: Rf 0.39 (dichloromethane: methanol: 28% ammonia water=40:10:1);

NMR (CDCl₃): δ 0.88 (t, J=7.2 Hz, 6H), 1.29-1.81 (m, 10H), 1.90-2.00 (m,2H), 2.12-2.22 (m, 2H), 2.41-2.53 (m, 6H), 2.60-2.72 (m, 3H), 2.78-2.93(m, 3H), 4.71 (s, 4H), 6.96 (s, 2H), 6.98 (s, 2H)

Example 531-{4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]phenyl}-N,N-bis(1H-imidazol-2-ylmethyl)methaneamine(Compound 53)

The same procedure as a series of reactions of Example 16→Example18→Example 19 was carried out, except that1-(1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-imidazol-2-yl)methaneaminewas used or a corresponding amine compound was used in place of it inthe process of Example 16, to obtain the title compound having thefollowing physical properties.

Description: amorphous;

TLC: Rf 0.22 (chloroform:methanol: 28% ammonia water=80:10:1);

NMR (DMSO-D₆): δ 0.92-1.28 (m, 5H), 1.35-1.60 (m, 7H), 1.61-1.77 (m,4H), 2.10-2.23 (m, 1H), 2.25 (s, 2H), 2.32-2.48 (m, 6H), 3.44-3.51 (m,4H), 3.55 (s, 4H), 6.72-7.16 (m, 4H), 7.21 (d, J=7.80 Hz, 2H), 7.32 (d,J=7.80 Hz, 2H), 11.61-12.37 (m, 2H).

Example 54(1) to Example 54(2)

The reaction was carried out in the order of Example 3→Example 5→Example6→Example 10, using a corresponding amine in place of 4-formylbenzoicacid and t-butoxycarbonyl-1,4-butylenediamine in Example 3 and using acorresponding amine in place of diimidazoleamine in Example 6, to obtainthe title compound.

Example 54(1)1-[4-(2,8-diazaspiro[4.5]dec-2-ylcarbonyl)phenyl]-N,N-bis(1H-imidazol-2-ylmethyl)methaneamine(Compound 54-1)

Description: amorphous;

TLC: Rf 0.60 (chloroform:methanol: 28% ammonia water=80:20:4);

NMR (DMSO-D₆): δ 1.21-1.36 (m, 2H), 1.37-1.54 (m, 2H), 1.61-1.81 (m,2H), 2.55-2.76 (m, 3H), 3.10-3.27 (m, 3H), 3.38-3.51 (m, 2H), 3.51-3.56(m, 2H), 3.56-3.66 (m, 4H), 6.71-6.97 (m, 2H), 6.99-7.24 (m, 2H),7.34-7.54 (m, 4H), 11.85-12.20 (m, 2H)

Example 54(2)1-{4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)carbonyl]phenyl}-N,N-bis(1H-imidazol-2-ylmethyl)methaneamine(Compound 54-2)

Description: amorphous;

TLC: Rf 0.64 (chloroform:methanol: 28% ammonia water=80:20:4);

NMR (DMSO-D₆): δ 1.15 (m, 5H), 1.40 (m, 2H), 1.56 (m, 3H), 1.71 (m, 6H),2.27 (m, 2H), 2.43 (m, 2H), 3.23 (m, 3H), 3.45 (m, 2H), 3.54 (m, 2H),3.58 (m, 4H), 6.97 (m, 4H), 7.43 (m, 4H), 12.00 (m, 2H)

Biological Examples

Efficacy of the compound of the present invention, for example the factthat the compound of the present invention has CXCR4 antagonisticactivity, has been demonstrated by the following experiment.

A measuring method of the present invention was modified to improveaccuracy and/or sensitivity of the measurement for evaluating thecompound of the present invention. The detailed experimental methods areshown bellow.

As mentioned above, a more direct procedure is a screening a compoundthat prevents for HIV from binding to CXCR4, which is a receptor on CD4+cell, on an assay system using HIV viruses. However, using HIV virusesfor a large-scale screening is not practical due to its difficulthandling. On the other hand, both of T cell-directed (X4) HIV-1 andSDF-1 bind to CXCR4 and therefore CXCR4 binding sites at both ofHIV-side and SDF-1-side as well as SDF-1- and HIV-binding sites at theCXCR4 side may presumably have any common characteristics. Thus, to finda compound inhibiting absorption of HIV viruses to a cell that is adifferent mechanism from those of pre-existing anti-AIDS drugs (reversetranscriptase inhibitors and protease inhibitors), an assay system usingan endogenous ligand for CXCR4, SDF-1 instead of HIV may be available.

Specifically, as a system of screening a compound that inhibits thebinding between SDF-1 and CXCR4, for example a system of measuring thebinding between iodine-labeled SDF-1 and a human T cell strain in whichCXCR4 is known to be expressed is operable. The identical idea ispossible since macrophage (R5) HIV and RANTES, MIP-1α, and MIP-1β allbind to CCR5.

[Test Methods]

Test Example 1 Study for Inhibition of Binding Human SDF-1 to CEM Cells

To human T cell strain CEM cells in a binding buffer (containing HEPESand BSA), the test compound and ¹²⁵I-SDF-1 (NEN) were added and themixture was incubated at 4° C. for 60 minutes. The reacted CEM cellswere rapidly filtrated with a GF/B membrane filter plate (Packard) toadsorb. The plate was washed with PBS three times and then dried.Microscint+20 (Packard) was added thereto. An amount of theradioactivity bound to the CEM cells was measured using Top Count(Packard) and inhibition (%) of the test compound was calculatedaccording to the following equation:Inhibition={(Et−Ea)/(Et−Ec)}×100

wherein

Et: amount of radioactivity when the test compound is not added,

Ec: amount of radioactivity when non-radioactive SDF-1 (Pepro Tech) isadded in an amount of 1000 times as much as ¹²⁵I-SDF-1 as a testcompound, and

Ea: amount of radioactivity when the test compound is added.

All compounds of the present invention shown in the Example exhibitedinhibition of 50% or more in a concentration of 10 μM. For example, IC₅₀value for compound 10 was 27 nM.

Test Example 2 Measuring of Influence of a Compound of the PresentInvention on Blood Pressure and Heart Rate

A rat was anesthetized with urethane (1.2 g/kg subcutaneousadministration). After neck midline dissection, a catheter for measuringblood pressure was inserted into a right common carotid artery. Then,after dissecting inguinal region, a catheter for chemical injection wasinserted into a femoral vein and fixed. A catheter for measurement ofblood pressure was connected to a pressure transducer and then thepressure waveform was recorded on a thermal writing pen recorder throughan amplifier for strain compression (AP-641G (manufactured by NIHONKOHDEN CORPORATION)). In this case, regarding a heart rate, a valuethrough an cardiotachometer (AT-601G (manufactured by NIHON KOHDENCORPORATION)) using the pressure waveform obtained from the amplifierfor strain compression as a trigger was recorded on a thermal writingpen recorder. The test compound was dissolved in a 10% solubilizingagent/physiological saline solution (volume ratio of polyoxyethylenehydroxystearate:propylene glycol:physiological saline=7:3:190) so as toadjust the concentration to 0.1, 0.3, 1, 3 or 10 mg/mL to preparesolutions. Each solution was intravenous administered at 1 mL/Kg throughthe caudal vein over about 10 seconds. Accumulative administration ofstepwise increasing of a dosage was carried out to an individual.

In case of administration of the compound 19, when the concentration was3 mg/kg or less, the effect of decreasing blood pressure was notrecognized, and a rate of increase in the heart rate was 2% when 10mg/kg of the test compound is administered, and there was no influenceon the heart rate in case of a low dosage.

Formulation Examples Formulation Example 1

4-({[4-(dipropylamino)butyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzoamide(200 g), calcium carboxymethyl cellulose (disintegrant, 20.0 g),magnesium stearate (lubricants, 10.0 g) and microcrystalline cellulose(870 g) were mixed by a conventional method and then compressed toobtain 10,000 tablets each containing 20 mg of an active ingredient.

Formulation Example 2

4-({[4-(dipropylamino)butyl]amino}methyl)-N,N-bis(1H-imidazol-2-ylmethyl)benzoamide(100 g), mannitol (2 kg) and distilled water (50 L) were mixed by aconventional method and filtered with a dust filter, and then eachampoule was filled with 5 mL of the obtained mixture and subjected toheat sterilization in an autoclave to obtain 10,000 ampoules eachcontaining 10 mg of an active ingredient.

INDUSTRIAL APPLICABILITY

The compound of the present invention has CXCR4 antagonistic activityand is therefore useful as a preventive and/or therapeutic agent forCXCR4-mediated diseases. Accordingly, the compound of the presentinvention can be available as a drug. For example, the compound of thepresent invention is useful as a preventive and/or therapeutic agent forinflammatory and immune diseases (for example, rheumatoid arthritis,arthritis, retinopathy, pulmonary fibrosis, transplanted organrejection, etc.), allergic diseases, infections (for example, humanimmunodeficiency virus infection, acquired immunodeficiency syndrome,etc.), psychoneurotic diseases, cerebral diseases, cardiovasculardisease, metabolic diseases, and cancerous diseases (for example,cancer, cancer metastasis, etc.), or an agent for regeneration therapy.

1. A compound represented by formula (I-0):

wherein A¹ and A² each independently represents an imidazole which maybe substituted with T; B¹ and B² each independently represents a C1-4alkylene group which may be substituted with T; E represents a phenylenewhich may be substituted with T; L represents a C1-4 alkylene groupwhich may be substituted with T; J⁰ represents 2,8 diazaspiro[4,5]decanewhich may be substituted with T;

represents a single bond; D represents a nitrogen atom, G represents aCO or CH₂, T represents (1) C1-8 alkyl group, C2-8 alkenyl group or C2-8alkynyl group, (2) methylidene, ethylidene, propylidene, butylidene,pentylidene, hexylidene, heptylidene, octylidene group, or isomerthereof, (3) C3-15 monocyclic or polycyclic carbocyclic ring or 3- to15-membered monocyclic or polycyclic heterocyclic ring having, as ahetero atom, 1 to 4 nitrogen atom(s), 1 to 2 oxygen atom(s) and/or 1 to2 sulfur atom(s), (4) cyclopropylmethyl, cyclopentylmethyl,cyclohexylmethyl, phenylmethyl, naphthylmethyl, pyridinylmethyl,cyclopropylethyl, cyclopentylethyl, cyclohexylethyl, phenylethyl,naphthylethyl, pyridinylethyl, cyclopropylpropyl, cyclopentylpropyl,cyclohexylpropyl, phenylmethyl, phenylpropyl, naphthylpropyl,pyridinylpropyl, (5) hydroxyl group, (6) methoxy, ethoxy, propoxy,isopropoxy, butoxy, isobutoxy, tert-butoxy, pentoxy, hexyloxy,heptyloxy, octyloxy, propenyloxy, butenyloxy, pentenyloxy, hexenyloxy,propynyloxy, butynyloxy, pentynyloxy, hexynyloxy, (7) cyclopropyloxy,cyclopentyloxy, cyclohexyloxy, phenoxy, naphthyloxy or pyridinyloxy, (8)cyclopentylmethoxy, cyclohexylmethoxy or phenylmethoxy, (9) mercaptogroup, (10) methylthio, ethylthio, propylthio, isopropylthio, butylthio,isobutylthio, tert-butylthio, pentylthio, hexylthio, heptylthio,octylthio, propenylthio, butenylthio, pentenylthio, hexenylthio,propynylthio, butynylthio, pentynylthio or hexynylthio, (11)cyclopropylthio, cyclopentylthio, cyclohexylthio, phenylthio,naphthylthio or pyridinylthio, (12) cyclopentylmethylthio,cyclohexylmethylthio or phenylmethylthio, (13) methylsulfinyl,ethylsulfinyl, propylsulfinyl, isopropylsulfinyl, butylsulfinyl,isobutylsulfinyl, tert-butylsulfinyl, pentylsulfinyl, hexylsulfinyl,heptylsulfinyl, octylsulfinyl, propenylsulfinyl, butenylsulfinyl,pentenylsulfinyl, hexenylsulfinyl, propynylsulfinyl, butynylsulfinyl,pentynylsulfinyl or hexynylsulfinyl, (14) cyclopropylsulfinyl,cyclopentylsulfinyl, cyclohexylsulfinyl, phenylsulfinyl,naphthylsulfinyl or pyridinylsulfinyl, (15) cyclopentylmethylsulfinyl,cyclohexylmethylsulfinyl or phenylmethylsulfinyl, (16) methylsulfonyl,ethylsulfonyl, propylsulfonyl, isopropylsulfonyl, butylsulfonyl,isobutylsulfonyl, tert-butylsulfonyl, pentylsulfonyl, hexylsulfonyl,heptylsulfonyl, octylsulfonyl, propenylsulfonyl, butenylsulfonyl,pentenylsulfonyl, hexenylsulfonyl, propynylsulfonyl, butynylsulfonyl,pentynylsulfonyl or hexynylsulfonyl, (17) cyclopropylsulfonyl,cyclopentylsulfonyl, cyclohexylsulfonyl, phenylsulfonyl,naphthylsulfonyl or pyridinylsulfonyl, (18) cyclopentylmethylsulfonyl,cyclohexylmethylsulfonyl or phenylmethylsulfonyl, (19) methanoyloxy,ethanoyloxy, propanoyloxy, isopropanoyloxy, butanoyloxy, isobutanoyloxy,tert-butanoyloxy, pentanoyloxy, hexanoyloxy, heptanoyloxy, octanoyloxy,propenoyloxy, butenoyloxy, pentenoyloxy, hexenoyloxy, propynoyloxy,butynoyloxy, pentynoyloxy or hexynoyloxy, (20) cyclopropylcarbonyloxy,cyclopentylcarbonyloxy, cyclohexylcarbonyloxy, phenylcarbonyloxy,naphthylcarbonyloxy or pyridinylcarbonyloxy, (21)cyclopentylmethanoyloxy, cyclohexylmethanoyloxy or phenylmethanoyloxy,(22) methanoyl, ethanoyl, propanoyl, isopropanoyl, butanoyl,isobutanoyl, tert-butanoyl, pentanoyl, hexanoyl, heptanoyl, octanoyl,propenoyl, butenoyl, pentenoyl, hexenoyl, propynoyl, butynoyl, pentynoylor hexynoyl, (23) cyclopropylcarbonyl, cyclopentylcarbonyl,cyclohexylcarbonyl, phenylcarbonyl, naphthylcarbonyl orpyridinylcarbonyl, (24) cyclopentylmethanoyl, cyclohexylmethanoyl orphenylmethanoyl, (25) oxo group, (26) thioxo group, (27) sulfino group,(28) sulfo group, (29) amino group, (30) methylamino, ethylamino,propylamino, isopropylamino, butylamino, isobutylamino, tert-butylamino,pentylamino, hexylamino, heptylamino, octylamino, dimethylamino,diethylamino, dipropylamino, dibutylamino, dipentylamino, dihexylamino,diheptylamino, dioctylamino, N-methyl-N-ethylamino, cyclopropylamino,cyclopentylamino, cyclohexylamino, phenylamino, diphenylamino,dibenzylamino, N-phenyl-N-methylamino, N-phenyl-N-ethylamino,N-benzyl-N-methylamino, N-benzyl-N-ethylamino orN-cyclohexyl-N-propylamino, (31) sulfamoyl group, (32)N-methylsulfamoyl, N-ethylsulfamoyl, N-propylsulfamoyl,N-isopropylsulfamoyl, N-butylsulfamoyl, N-isobutylsulfamoyl,N-(tert-butyl)sulfamoyl, N-pentylsulfamoyl, N-hexylsulfamoyl,N-heptylsulfamoyl, N-octylsulfamoyl, N,N-dimethylsulfamoyl,N,N-diethylsulfamoyl, N,N-dipropylsulfamoyl, N,N-dibutylsulfamoyl,N,N-dipentylsulfamoyl, N,N-dihexylsulfamoyl, N-methyl-N-ethylsulfamoyl,N-cyclopropylsulfamoyl, N-cyclopentylsulfamoyl, N-cyclohexylsulfamoyl,N-phenylsulfamoyl, N,N-diphenylsulfamoyl, N,N-dibenzylsulfamoyl,N-phenyl-N-methylsulfamoyl, N-phenyl-N-ethylsulfamoyl,N-benzyl-N-methylsulfamoyl, N-benzyl-N-ethylsulfamoyl orN-cyclohexyl-N-propylamino, (33) carboxy group, (34) —COO-aliphatichydrocarbon group having 1 to 8 carbon atom(s), (35)cyclopropyloxycarbonyl, cyclopentyloxycarbonyl, cyclohexyloxycarbonyl,phenoxycarbonyl, naphthyloxycarbonyl or pyridinyloxycarbonyl, (36)cyclopentylmethoxycarbonyl, cyclohexylmethoxycarbonyl orphenylmethoxycarbonyl, (37) carbamoyl group, (38) N-methylcarbamoyl,N-ethylcarbamoyl, N-propylcarbamoyl, N-isopropylcarbamoyl,N-butylcarbamoyl, N-isobutylcarbamoyl, N-(tert-butyl)carbamoyl,N-pentylcarbamoyl, N-hexylcarbamoyl, N-heptylcarbamoyl,N-octylcarbamoyl, N-cyclopropylcarbamoyl, N-cyclopentylcarbamoyl,N-cyclohexylcarbamoyl, N-phenylcarbamoyl, N,N-dimethylcarbamoyl,N,N-diethylcarbamoyl, N,N-dipropylcarbamoyl, N,N-dibutylcarbamoyl,N,N-dipentylcarbamoyl, N,N-dihexylcarbamoyl, N-methyl-N-ethylcarbamoyl,N,N-diphenylcarbamoyl, N,N-dibenzylcarbamoyl,N-phenyl-N-methylcarbamoyl, N-phenyl-N-ethylcarbamoyl,N-benzyl-N-methylcarbamoyl or N-benzyl-N-ethylcarbamoyl, (39)methanoylamino, ethanoylamino, propanoylamino, isopropanoylamino,butanoylamino, isobutanoylamino, tert-butanoylamino, pentanoylamino,hexanoylamino, heptanoylamino, octanoylamino, propenoylamino,butenoylamino, pentenoylamino, hexenoylamino, propynoylamino,butynoylamino, pentynoylamino or hexynoylamino, (40)cyclopropylcarbonylamino, cyclopentylcarbonylamino,cyclohexylcarbonylamino, phenylcarbonylamino, naphthylcarbonylamino orpyridinylcarbonylamino, (41) cyclopentylmethanoylamino,cyclohexylmethanoylamino or phenylmethanoylamino, (42)methylsulfonylamino, ethylsulfonylamino, propylsulfonylamino,isopropylsulfonylamino, butylsulfonylamino, isobutylsulfonylamino,tert-butylsulfonylamino, pentylsulfonylamino, hexylsulfonylamino,heptylsulfonylamino, octylsulfonylamino, propenylsulfonylamino,butenylsulfonylamino, pentenylsulfonylamino, hexenylsulfonylamino,propynylsulfonylamino, butynylsulfonylamino, pentynylsulfonylamino orhexynylsulfonyl, (43) cyclopropylsulfonylamino,cyclopentylsulfonylamino, cyclohexylsulfonylamino, phenylsulfonylamino,naphthylsulfonylamino or pyridinylsulfonyl, (44)cyclopentylmethylsulfonylamino, cyclohexylmethylsulfonylamino orphenylmethylsulfonyl, (45) cyano group, (46) hydrazino group, (47) nitrogroup, (48) nitroso group, (49) imino group, (50) methylimino,ethylimino, propylimino, isopropylimino, butylimino, isobutylimino,(tert-butyl)imino, pentylimino, hexylimino, heptylimino, octylimino,cyclopropylimino, cyclopentylimino, cyclohexylimino, phenylimino,benzylimino, hydroxyimino, ethoxyimino, propoxyimino, isopropoxyimino,butoxyimino, cyclopentoxyimino, cyclohexyloxyimino, phenoxyimino orbenzyloxyimino, (51) halogen atom, (52) methyl group substituted with 1to 3 halogen atom(s), and (53) methoxy group substituted with 1 to 3halogen atom(s), which may be substituted with one or more groupsselected from above (1)-(53); or a salt thereof, or an N-oxide thereof.2. The compound according to claim 1, which is represented by formula(I-4):

wherein ring A^(1B) and ring A^(2B) each independently representsimidazole which may be substituted with T; ring E^(A) represents aphenylene which may be substituted with T; and other symbols and T havethe same meanings as described in claim
 1. 3. The compound according toclaim 2, which is represented by formula (I-4-1b):

wherein L^(A1) represents C1-4 alkylene group which may be substitutedwith T; R¹ represents (1) aliphatic hydrocarbon group having 1 to 8carbon atom(s) which may be substituted with T, (2) C3-15 monocyclic orpolycyclic carbocyclic ring or 3- to 15-membered monocyclic orpolycyclic hetrocyclic ring having, as a hetero atom, 1 to 4 nitrogenatom(s), 1 to 2 oxygen atom(s) and/or 1 to 2 sulfur atom(s), which maybe substituted with T, (3) T; and other symbols and T have the samemeanings as described in claim
 2. 4. The compound according to claim 2,which is represented by formula (I-4-2):

wherein L^(A1) represents C1-4 alkylene group which may be substitutedwith T; R¹ represents (1) aliphatic hydrocarbon group having 1 to 8carbon atom(s) which may be substituted with T, (2) C3-15 monocyclic orpolycyclic carbocyclic ring or 3- to 15-membered monocyclic orpolycyclic hetrocyclic ring having, as a hetero atom, 1 to 4 nitrogenatom(s), 1 to 2 oxygen atom(s) and/or 1 to 2 sulfur atom(s), which maybe substituted with T, (3) T; and other symbols and T have the samemeanings as described in claim
 2. 5. The compound according to claim 1,which is represented by formula (I-8):

wherein ring A^(1A) and ring A^(2A) each independently representsimidazole which may be substituted with T; ring E^(A) represents aphenylene which may be substituted with T; L^(A) represents a C1-4alkylene group which may be substituted with T; and J⁰ T has the samemeaning as described in claim
 1. 6. The compound according to claim 1,which is4-[(2-cyclohexyl-2,8-diazaspiro[4.5]dec-8-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;N,N-bis(1H-imidazol-2-ylmethyl)-4-{[8-(2-thienylmethyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}benzamide;4-[(8-cycloheptyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;N,N-bis(1H-imidazol-2-ylmethyl)-4-({8-[(3-methyl-2-thienyl)methyl]-2,8-diazaspiro[4.5]dec-2-yl}methyl)benzamide;4-{[8-(1-ethylpropyl)-2,8-diazaspiro[4.5]dec-2-yl]methyl}-N,N-bis(1H-imidazol-2-ylmethyl)benzamide;N,N-bis(1H-imidazol-2-ylmethyl)-4-[(8-isobutyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]benzamide;or1-{4-[(8-cyclohexyl-2,8-diazaspiro[4.5]dec-2-yl)methyl]phenyl}-N,N-bis(1H-imidazol-2-ylmethyl)methaneamine.7. A compound represented by formula (I-8):

wherein ring A^(1A), ring A^(2A) each independently represents animidazole which may be substituted with C1-8 alkyl; E^(A) represents aphenylene which may be substituted with a halogen atom or a methylgroup: L^(A) represents —CH₂— or —CH₂—CH₂—; J⁰ represents

which may be substituted with T; R¹ represents a C1-8 alkyl group, aC3-8 monocyclic saturated carbocyclic ring or a C1-8 alkyl group whichis substituted with thiophene; and T represents (1) aliphatichydrocarbon group having 1 to 8 carbon atom(s), (2) C1-8 alkylidenegroup, (3) C3-15 monocyclic or polycyclic carbocyclic ring or 3- to15-membered monocyclic or polycyclic heterocyclic ring having, as ahetero atom, 1 to 4 nitrogen atom(s), 1 to 2 oxygen atom(s) and/or 1 to2 sulfur atom(s), (4) cyclopropylmethyl, cyclopentylmethyl,cyclohexylmethyl, phenylmethyl, naphthylmethyl, pyridinylmethyl,cyclopropylethyl, cyclopentylethyl, cyclohexylethyl, phenylethyl,naphthylethyl, pyridinylethyl, cyclopropylpropyl, cyclopentylpropyl,cyclohexylpropyl, phenylmethyl, phenylpropyl, naphthylpropyl,pyridinylpropyl, (5) hydroxyl group, (6) methoxy, ethoxy, propoxy,isopropoxy, butoxy, isobutoxy, tert-butoxy, pentoxy, hexyloxy,heptyloxy, octyloxy, propenyloxy, butenyloxy, pentenyloxy, hexenyloxy,propynyloxy, butynyloxy, pentynyloxy, hexynyloxy, (7) cyclopropyloxy,cyclopentyloxy, cyclohexyloxy, phenoxy, naphthyloxy or pyridinyloxy, (8)cyclopentylmethoxy, cyclohexylmethoxy or phenylmethoxy, (9) mercaptogroup, (10) methylthio, ethylthio, propylthio, isopropylthio, butylthio,isobutylthio, tert-butylthio, pentylthio, hexylthio, heptylthio,octylthio, propenylthio, butenylthio, pentenylthio, hexenylthio,propynylthio, butynylthio, pentynylthio or hexynylthio, (11)cyclopropylthio, cyclopentylthio, cyclohexylthio, phenylthio,naphthylthio or pyridinylthio, (12) cyclopentylmethylthio,cyclohexylmethylthio or phenylmethylthio, (13) methylsulfinyl,ethylsulfinyl, propylsulfinyl, isopropylsulfinyl, butylsulfinyl,isobutylsulfinyl, tert-butylsulfinyl, pentylsulfinyl, hexylsulfinyl,heptylsulfinyl, octylsulfinyl, propenylsulfinyl, butenylsulfinyl,pentenylsulfinyl, hexenylsulfinyl, propynylsulfinyl, butynylsulfinyl,pentynylsulfinyl or hexynylsulfinyl, (14) cyclopropylsulfinyl,cyclopentylsulfinyl, cyclohexylsulfinyl, phenylsulfinyl,naphthylsulfinyl or pyridinylsulfinyl, (15) cyclopentylmethylsulfinyl,cyclohexylmethylsulfinyl or phenylmethylsulfinyl, (16) methylsulfonyl,ethylsulfonyl, propylsulfonyl, isopropylsulfonyl, butylsulfonyl,isobutylsulfonyl, tert-butylsulfonyl, pentylsulfonyl, hexylsulfonyl,heptylsulfonyl, octylsulfonyl, propenylsulfonyl, butenylsulfonyl,pentenylsulfonyl, hexenylsulfonyl, propynylsulfonyl, butynylsulfonyl,pentynylsulfonyl or hexynylsulfonyl, (17) cyclopropylsulfonyl,cyclopentylsulfonyl, cyclohexylsulfonyl, phenylsulfonyl,naphthylsulfonyl or pyridinylsulfonyl, (18) cyclopentylmethylsulfonyl,cyclohexylmethylsulfonyl or phenylmethylsulfonyl, (19) methanoyloxy,ethanoyloxy, propanoyloxy, isopropanoyloxy, butanoyloxy, isobutanoyloxy,tert-butanoyloxy, pentanoyloxy, hexanoyloxy, heptanoyloxy, octanoyloxy,propenoyloxy, butenoyloxy, pentenoyloxy, hexenoyloxy, propynoyloxy,butynoyloxy, pentynoyloxy or hexynoyloxy, (20) cyclopropylcarbonyloxy,cyclopentylcarbonyloxy, cyclohexylcarbonyloxy, phenylcarbonyloxy,naphthylcarbonyloxy or pyridinylcarbonyloxy, (21)cyclopentylmethanoyloxy, cyclohexylmethanoyloxy or phenylmethanoyloxy,(22) methanoyl, ethanoyl, propanoyl, isopropanoyl, butanoyl,isobutanoyl, tert-butanoyl, pentanoyl, hexanoyl, heptanoyl, octanoyl,propenoyl, butenoyl, pentenoyl, hexenoyl, propynoyl, butynoyl, pentynoylor hexynoyl, (23) cyclopropylcarbonyl, cyclopentylcarbonyl,cyclohexylcarbonyl, phenylcarbonyl, naphthylcarbonyl orpyridinylcarbonyl, (24) cyclopentylmethanoyl, cyclohexylmethanoyl orphenylmethanoyl, (25) oxo group, (26) thioxo group, (27) sulfino group,(28) sulfo group, (29) amino group, (30) methylamino, ethylamino,propylamino, isopropylamino, butylamino, isobutylamino, tert-butylamino,pentylamino, hexylamino, heptylamino, octylamino, dimethylamino,diethylamino, dipropylamino, dibutylamino, dipentylamino, dihexylamino,diheptylamino, dioctylamino, N-methyl-N-ethylamino, cyclopropylamino,cyclopentylamino, cyclohexylamino, phenylamino, diphenylamino,dibenzylamino, N-phenyl-N-methylamino, N-phenyl-N-ethylamino,N-benzyl-N-methylamino, N-benzyl-N-ethylamino orN-cyclohexyl-N-propylamino, (31) sulfamoyl group, (32)N-methylsulfamoyl, N-ethylsulfamoyl, N-propylsulfamoyl,N-isopropylsulfamoyl, N-butylsulfamoyl, N-isobutylsulfamoyl,N-(tert-butyl)sulfamoyl, N-pentylsulfamoyl, N-hexylsulfamoyl,N-heptylsulfamoyl, N-octylsulfamoyl, N,N-dimethylsulfamoyl,N,N-diethylsulfamoyl, N,N-dipropylsulfamoyl, N,N-dibutylsulfamoyl,N,N-dipentylsulfamoyl, N,N-dihexylsulfamoyl, N-methyl-N-ethylsulfamoyl,N-cyclopropylsulfamoyl, N-cyclopentylsulfamoyl, N-cyclohexylsulfamoyl,N-phenylsulfamoyl, N,N-diphenylsulfamoyl, N,N-dibenzylsulfamoyl,N-phenyl-N-methylsulfamoyl, N-phenyl-N-ethylsulfamoyl,N-benzyl-N-methylsulfamoyl, N-benzyl-N-ethylsulfamoyl orN-cyclohexyl-N-propylamino, (33) carboxy group, (34) —COO-aliphatichydrocarbon group having 1 to 8 carbon atom(s), (35)cyclopropyloxycarbonyl, cyclopentyloxycarbonyl, cyclohexyloxycarbonyl,phenoxycarbonyl, naphthyloxycarbonyl or pyridinyloxycarbonyl, (36)cyclopentylmethoxycarbonyl, cyclohexylmethoxycarbonyl orphenylmethoxycarbonyl, (37) carbamoyl group, (38) N-methylcarbamoyl,N-ethylcarbamoyl, N-propylcarbamoyl, N-isopropylcarbamoyl,N-butylcarbamoyl, N-isobutylcarbamoyl, N-(tert-butyl)carbamoyl,N-pentylcarbamoyl, N-hexylcarbamoyl, N-heptylcarbamoyl,N-octylcarbamoyl, N-cyclopropylcarbamoyl, N-cyclopentylcarbamoyl,N-cyclohexylcarbamoyl, N-phenylcarbamoyl, N,N-dimethylcarbamoyl,N,N-diethylcarbamoyl, N,N-dipropylcarbamoyl, N,N-dibutylcarbamoyl, N,Ndipentylcarbamoyl, N,N-dihexylcarbamoyl, N-methyl-N-ethylcarbamoyl,N,N-diphenylcarbamoyl, N,N-dibenzylcarbamoyl,N-phenyl-N-methylcarbamoyl, N-phenyl-N-ethylcarbamoyl,N-benzyl-N-methylcarbamoyl or N-benzyl-N-ethylcarbamoyl, (39)methanoylamino, ethanoylamino, propanoylamino, isopropanoylamino,butanoylamino, isobutanoylamino, tert-butanoylamino, pentanoylamino,hexanoylamino, heptanoylamino, octanoylamino, propenoylamino,butenoylamino, pentenoylamino, hexenoylamino, propynoylamino,butynoylamino, pentynoylamino or hexynoylamino, (40)cyclopropylcarbonylamino, cyclopentylcarbonylamino,cyclohexylcarbonylamino, phenylcarbonylamino, naphthylcarbonylamino orpyridinylcarbonylamino, (41) cyclopentylmethanoylamino,cyclohexylmethanoylamino or phenylmethanoylamino, (42)methylsulfonylamino, ethylsulfonylamino, propylsulfonylamino,isopropylsulfonylamino, butylsulfonylamino, isobutylsulfonylamino,tert-butylsulfonylamino, pentylsulfonylamino, hexylsulfonylamino,heptylsulfonylamino, octylsulfonylamino, propenylsulfonylamino,butenylsulfonylamino, pentenylsulfonylamino, hexenylsulfonylamino,propynylsulfonylamino, butynylsulfonylamino, pentynylsulfonylamino orhexynylsulfonyl, (43) cyclopropylsulfonylamino,cyclopentylsulfonylamino, cyclohexylsulfonylamino, phenylsulfonylamino,naphthylsulfonylamino or pyridinylsulfonyl, (44)cyclopentylmethylsulfonylamino, cyclohexylmethylsulfonylamino orphenylmethylsulfonyl, (45) cyano group, (46) hydrazino group, (47) nitrogroup, (48) nitroso group, (49) imino group, (50) methylimino,ethylimino, propylimino, isopropylimino, butylimino, isobutylimino,(tert-butyl)imino, pentylimino, hexylimino, heptylimino, octylimino,cyclopropylimino, cyclopentylimino, cyclohexylimino, phenylimino,benzylimino, hydroxyimino, ethoxyimino, propoxyimino, isopropoxyimino,butoxyimino, cyclopentoxyimino, cyclohexyloxyimino, phenoxyimino orbenzyloxyimino, (51) halogen atom, (52) methyl group substituted with 1to 3 halogen atom(s), and (53) methoxy group substituted with 1 to 3halogen atom(s), which may be substituted with one or more groupsselected from above (1)-(53); or a salt thereof, or an N-oxide thereof.8. A pharmaceutical composition comprising a compound represented byformula (I-0) described in claim 1 or formula (I-8) described in claim7, or a salt thereof.
 9. A pharmaceutical composition comprising acompound represented by formula (I-0) described in claim 1 or formula(I-8) described in claim 7, a salt thereof, and one or more agentsselected from a reverse transcriptase inhibitor and a protease inhibitorwherein said reverse transcriptase inhibitor is at least one compoundselected from the group consisting of zidovudine, didanosine,zalcitabine, stavudine, lamivudine, abacavir, didanosine, adefovir,dipivoxil, emtricitabine, tenofovir, nevirapine, delavirdine, efavirenz,and capravirine, and wherein said protease inhibitor is at least onecompound selected from the group consisting of indinavir, ritonavir,nelfinavir, saquinavir, amprenavir, lopinavir, atazanavir,fosamprenavir, and tipranavir.
 10. A method of treating CXCR4-mediateddiseases selected from the group consisting of human immunodeficiencyvirus infection, acquired immunodeficiency syndrome, small cell lungcancer, breast cancer, melanoma, chronic leukemias, multiple myeloma,malignant myeloma, pancreatic cancer, prostate cancer, acute and chronicleukemia, rheumatoid arthritis, collagen-induced arthritis, diabeticretinopathy, and pulmonary fibrosis, which comprises administering aneffective dosage of a compound represented by formula (I-0) described inclaim 1 or formula (I-8) described in claim 7, a salt thereof, or anN-oxide thereof to the mammal.
 11. A method of treating humanimmunodeficiency virus infection, which comprises administering aneffective dosage of a compound represented by formula (I-0) described inclaim 1 or formula (I-8) described in claim 7, or a salt thereof, or anN-oxide thereof to a mammal.